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CN-122004220-A - Seed coating agent and application thereof, rooting promoting method and salt stress relieving method

CN122004220ACN 122004220 ACN122004220 ACN 122004220ACN-122004220-A

Abstract

The invention relates to the technical field of agricultural biology, and discloses a plant seed coating agent and application thereof, a rooting promoting method and a salt stress relieving method. The seed coating agent of the invention has the active ingredient of at least one of L-phenylalanine, derivatives of L-phenylalanine or precursor substances capable of being metabolized and converted into L-phenylalanine in plants. The content of the active ingredient in the seed coating agent is 0.1 to 10 percent by weight percent. The seed coating agent can effectively improve the crop emergence rate, the seedling rate and the seedling quality of the saline land, and provides a new environment-friendly and efficient strategy for solving the problems of difficult emergence and difficult seedling protection in the agricultural production of the saline land.

Inventors

  • TAN YANNING
  • QI YUCHENG
  • HUANG YUBO
  • Li Manti
  • YANG XULEI
  • WU LEKANG
  • LAN LIQIONG
  • LIU FURONG

Assignees

  • 三亚市国家耐盐碱水稻技术创新中心
  • 湖南杂交水稻研究中心

Dates

Publication Date
20260512
Application Date
20251216

Claims (10)

  1. 1. A seed coating agent for relieving saline-alkali stress of plants, which is characterized in that the seed coating agent contains an active ingredient, wherein the active ingredient contains at least one of L-phenylalanine, a physiologically acceptable derivative of the L-phenylalanine and a precursor substance capable of being metabolically converted into the L-phenylalanine in plants; preferably, the derivative is at least one selected from the group consisting of salt derivatives of L-phenylalanine, amide derivatives of L-phenylalanine and ester derivatives of L-phenylalanine; Preferably, the derivative is selected from at least one of L-phenylalanine amide, L-phenylalanine hydrochloride, L-phenylalanine sodium, L-2-phenylalanine, and L-phenylalanine ethyl ester hydrochloride.
  2. 2. The seed coating agent of claim 1, wherein the active ingredient is L-phenylalanine.
  3. 3. The seed coating agent according to claim 1, wherein the content of the active ingredient is 0.25g to 2.5g with respect to 100ml of the seed coating agent; Preferably, the content of the active ingredient is 0.5 g-2 g relative to 100ml of the seed coating agent.
  4. 4. A seed coating agent according to claim 1 or 2, wherein the active ingredients are L-phenylalanine and DL-alpha-tocopheryl acetate; preferably, the weight ratio of the L-phenylalanine to the DL-alpha-tocopheryl acetate is 1 (0.67-2), more preferably 1 (0.75-1.5).
  5. 5. A seed coating agent according to any one of claims 1 to 4, further comprising an agriculturally acceptable carrier; preferably, the carrier comprises at least one of a binder, a film former, a dispersant, and a colorant; Preferably, the binder is xanthan gum; Preferably, the film former is polyvinyl alcohol.
  6. 6. Use of a seed coating as claimed in any one of claims 1 to 5 in the manufacture of a plant growth regulator for alleviating rooting inhibition in plants under salt and alkaline stress.
  7. 7. The use according to claim 6, wherein said plant is selected from at least one of the group consisting of gramineae, solanaceae, umbelliferae, cruciferae and leguminosae.
  8. 8. A method for promoting rooting of plant seeds under saline-alkali stress, which is characterized in that the method uses the seed coating agent as defined in any one of claims 1-5 to carry out coating treatment on the seeds; preferably, the seed is a rice seed.
  9. 9. The method of claim 8, wherein the salt stress in the salt-tolerant stress is a soil environment and/or a water environment with a NaCl concentration between 25mM and 100 mM; And/or the alkali stress in the alkali stress is soil environment and/or water environment with pH value between 8.5 and 9.5.
  10. 10. A method for inhibiting jasmonic acid synthesis in plant roots after sowing to relieve salt and alkali stress, which is characterized in that the method is carried out by applying an effective salt and alkali stress relieving amount of active ingredients, wherein the active ingredients are L-phenylalanine or L-phenylalanine and DL-alpha-tocopheryl acetate.

Description

Seed coating agent and application thereof, rooting promoting method and salt stress relieving method Technical Field The invention relates to the technical field of agricultural biology, in particular to a seed coating agent for relieving saline-alkali stress and application thereof, a method for promoting rooting of rice seeds under salt stress and a method for inhibiting synthesis of jasmonic acid in rice root systems to relieve salt stress. Background Soil salinization is an increasingly serious global agricultural ecological problem, severely restricting crop production. It is counted that about 10 hundred million hectares of land in the world are affected by salinization and the area is still expanding due to improper irrigation, rising sea level, etc. Not only are main grain crops such as rice, wheat and corn deeply affected, but also important economic vegetables such as tomatoes, carrots, cabbage, cabbages and oil crops such as soybeans and the like, also show the problems of difficult germination and emergence, hindered seedling growth and the like in saline-alkali soil, and finally result in serious yield reduction. The direct seeding cultivation technology can remarkably save labor, water resources and production cost, and is regarded as a light and simple cultivation mode with great popularization potential in various crops. However, in saline-alkali soil, the germinated seed sprouts and roots are exposed to saline soil without buffer, so that the seedling rate is greatly reduced. Early research results of the research team show that radicle elongation of various crops can be obviously inhibited under the saline-alkali stress environment. Therefore, the establishment of a strong root system of seeds under the stress of saline and alkaline is promoted, the seedling rate is ensured, and the method has become a core commonality task for promoting the agricultural development of saline and alkaline land. In order to relieve the inhibition of saline-alkali stress on the growth of crops in bud phase, the prior art mainly expands around three strategies: and firstly, physical and chemical soil improvement. The sodium ions in the soil are replaced by adding substances such as gypsum, organic fertilizer and the like. Although the method has a certain effect, the cost is high, the engineering quantity is huge, the effect is difficult to last, and the method is not suitable for large-scale agricultural application. And secondly, genetically improving and cultivating saline-alkali tolerant varieties. This is one of the fundamental approaches to solving the saline-alkali stress. However, the salt-tolerant alkalinity of crops is a complex quantitative trait controlled by multiple genes, the breeding period is long, and the urgent requirement of the rapid growth of the current salinized land cannot be met due to the tradeoff difficulty of agronomic traits. Thirdly, the plant growth regulator is used. At present, regulators such as auxins (naphthalene acetic acid, indolebutyric acid and the like) or compound regulators which are commonly used for promoting root systems can effectively stimulate rooting under normal conditions, but under the specific adverse condition of saline-alkali stress, the root promoting effect is greatly reduced due to physiological disturbance of the whole plant, and the effects are unstable and lack of pertinence. Secondly, partial chemical regulator has the potential risks of long residual time in soil, possibly influencing environmental microorganisms and the like, and does not accord with the development trend of green agriculture. In recent years, the central role of the phytohormone signaling network in stress response has been gradually revealed. Jasmonic acid (Jasmonic Acid, JA) is a key adversity signaling molecule, whose role in plant resistance to biotic stress has been widely recognized, however its regulatory mechanism for root system construction in abiotic stress (e.g. saline-alkali stress) is not clear. The early research results of the research team show that under 50-100 mM NaCl salt stress or Na 2CO3/NaHCO3 alkali stress with pH of 9.0-9.5, the root system of crops such as rice and the like can generate a short and severe JA content burst peak, and the occurrence time of the burst peak is highly consistent with the critical window period of the growth inhibition of the root system. This finding strongly suggests that saline-alkali stress induced JA explosive accumulation is a key physiological event directly leading to stunted rice radicle growth. However, no environment-friendly and efficient technology capable of safely and effectively regulating the process to promote rooting and seedling formation of crops under saline-alkali stress exists at present. Disclosure of Invention The invention aims to provide a plant growth regulating seed coating agent for enhancing salt and alkali resistance of plants. The invention is developed based on the idea that the inventor c