CN-122004221-A - Application of dopamine synthesis inhibitor in pest control of prodenia litura

Abstract

The invention belongs to the technical field of pest control, and for the first time discloses application of tyrosine hydroxylase serving as a target spot in controlling spodoptera litura pests, and a technology for efficiently controlling spodoptera litura is developed by screening a dopamine synthesis inhibitor AMPT aiming at the tyrosine hydroxylase. The technology utilizes the function of inhibiting dopamine synthesis by injecting AMPT into the bodies of the four-instar larvae of the noctuid, so that the melanin deposition of the exoskeleton of the larvae is reduced, the body surface color is lightened, and the exercise capacity is obviously reduced. On one hand, the reduction of melanin deposition weakens the environment adaptability of the pests and increases the exposure risk of the pests in natural environment, and on the other hand, the reduction of the exercise capacity directly influences the migration behavior of the pests and limits the population diffusion and hazard range of the pests. The dual action mechanism reduces the environmental adaptability and the viability of pests together, and provides a new strategy for green prevention and control.

Inventors

• WEN JING
• ZHANG ZHUOYING
• LIU BINYAO
• LI JIAXIN
• YAO TINGTING
• JIN TINGTING
• YU XIAOQIANG

Assignees

• 华南师范大学

Dates

Publication Date

20260512

Application Date

20260106

Claims (7)

1. 1. The application of a dopamine synthesis inhibitor in preparing a preparation for preventing and controlling prodenia litura is characterized in that the dopamine synthesis inhibitor is alpha-methyl-DL-tyrosine methyl ester hydrochloride.
2. 2. The use according to claim 1, characterized in that the formulation has α -methyl-DL-tyrosine methyl ester hydrochloride as the sole active ingredient.
3. 3. The use according to claim 1, wherein the formulation is for one or both of the following applications: a. Reducing melanin deposition of exoskeleton of pests and reducing immune defenses of the pests; b. Reducing pest movement ability.
4. 4. The use according to claim 1, wherein the formulation is administered to prodenia litura by injection.
5. 5. A method for controlling prodenia litura, characterized in that an effective dose of alpha-methyl-DL-tyrosine methyl ester hydrochloride is applied to the prodenia litura.
6. 6. The method of claim 5, wherein the mode of administration is injection.
7. 7. The method of claim 6, wherein the α -methyl-DL-tyrosine methyl ester hydrochloride is injected after dissolving in PBS buffer.

Description

Application of dopamine synthesis inhibitor in pest control of prodenia litura Technical Field The invention belongs to the technical field of pest control, and particularly discloses application of a dopamine synthesis inhibitor in pest control of prodenia litura. Background The prodenia litura (Spodoptera litura), also called the spodoptera litura, is an important agricultural pest widely distributed in tropical and subtropical areas, has wide larva feeding habits and large feeding capacity, can harm more than 300 crops of more than 100 families including cruciferae, leguminosae and the like, and forms a serious threat to agricultural production and grain safety. At present, the prevention and the treatment still mainly depend on chemical pesticides, however, the long-term massive use not only leads to the enhancement of the drug resistance of pests, but also causes environmental pollution and ecological risks. Therefore, the development of novel prevention and control products with high efficiency, safety and environmental friendliness becomes a key task in the current agricultural pest control field and has important significance for promoting agricultural sustainable development. The body surface color of the prodenia litura larva can form a mimicry with the surrounding environment, so that natural enemy identification is effectively avoided. Melanin is used as a key pigment component in the exoskeleton, not only participates in body tone adjustment, but also enhances the physical resistance of the exoskeleton and helps to resist pathogen infection. Furthermore, melanin deposition helps to mitigate the risk of solar radiation-induced DNA damage and cell death, and plays a role in thermoregulation. These adaptive features increase the difficulty in monitoring and controlling the pest while improving its viability. And under high population density, the activity of the prodenia litura is enhanced, the prodenia litura has long-distance migration capability, and the formed large population is in outbreak and disaster, so that huge agricultural and economic losses are caused for greedy gnawing of crops. Dopamine is an evolutionarily conserved catecholamine neurotransmitter, which is widely existing in insects, and participates in regulating and controlling various physiological and behavioral processes such as development, immunity, feeding, coupling, movement and the like of the insects through specific binding to receptors. Dopamine biosynthesis relies on the catalysis of two key rate limiting enzymes, tyrosine Hydroxylase (TH) and dopa decarboxylase (Ddc). AMPT, as a specific inhibitor of TH, can effectively block dopamine synthesis, thereby leading to reduced melanin deposition and reduced locomotor ability in the exoskeletons of the pests. The reduction of melanin deposition impairs its environmental adaptation, whereas impaired locomotor ability directly influences its migration behaviour. Therefore, the novel inhibitor is developed by targeting the dopamine synthesis pathway, and a novel approach is provided for green and accurate prevention and control of prodenia litura by simultaneously interfering a morphological adaptation and behavior diffusion dual mechanism. Disclosure of Invention The invention aims to overcome the defects of the existing prevention and control technology, and provides a compound AMPT which takes tyrosine hydroxylase as a target and inhibits dopamine synthesis and application thereof in preventing and controlling prodenia litura. According to the invention, by injecting the AMPT in the bodies of the prodenia litura larvae of four ages, the AMPT is proved to cause reduction of exoskeleton melanin deposition of the prodenia litura larvae for the first time, the body surface color becomes light, and the movement capability is obviously reduced. The artificial intervention changes the environment adaptability and the motion migration capability of the prodenia litura larvae, thereby effectively preventing and controlling pests and reducing the risk of explosive disaster. The method has the advantages of convenient operation, accurate and effective target points, green pollution-free property and the like, has wide application prospect, and can bring long-term environmental and economic dual benefits for agricultural production. The object of the first aspect of the invention is to provide the application of tyrosine hydroxylase as a target in pest control, wherein the dopamine synthesis inhibitor targeting the noctuid tyrosine hydroxylase is AMPT. The second aspect of the invention aims at providing an application of the dopamine synthesis inhibitor AMPT in preventing and controlling prodenia litura and/or preparing a prodenia litura prevention and control product. The object of a third aspect of the present invention is to provide a method for controlling pests. By injecting AMPT into the prodenia litura larvae, the deposition of melanin of the larvae exoskeleton is reduced, th