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CN-122004223-A - Benzenesulfonic acid anti-biological film preparation and preparation method and application thereof

CN122004223ACN 122004223 ACN122004223 ACN 122004223ACN-122004223-A

Abstract

The invention relates to a benzenesulfonic acid anti-biofilm preparation, which consists of, by mass, 10% -75% of benzenesulfonic acid, 10% -40% of propylene glycol, 5% -25% of gel matrix and stabilizer, 0.001% -0.2% of color development indicator and the balance of water, wherein the gel matrix and the stabilizer are colloidal silica, and the color development indicator is pharmaceutically or food-grade acceptable dye, and a preparation method of the benzenesulfonic acid anti-biofilm preparation and application thereof in bacterial biofilm removal. The benzenesulfonic acid has better biological film resisting activity than other traditional alkylsulfonic acid under relatively lower mass concentration, has better biological safety and no bad smell by adopting propanediol as an organic solvent and a permeation promoter in a plurality of biological film models, and has broad-spectrum antibacterial biological film characteristics and the application of the benzenesulfonic acid is covered in a plurality of fields such as medical treatment, sanitation, industry and the like.

Inventors

  • WU GUOYI
  • CHENG HAN
  • SU LIN
  • LIU YU

Assignees

  • 北生百翎(南通)生物科技有限公司

Dates

Publication Date
20260512
Application Date
20260202

Claims (10)

  1. 1. The benzenesulfonic acid anti-biological film preparation is characterized by comprising, by mass, 10% -75% of benzenesulfonic acid and 10% -40% of propylene glycol.
  2. 2. The benzenesulfonic acid biofilm formulation of claim 1, further comprising 5% -25% of a gel matrix and stabilizer, 0.001% -0.2% of a color-developing indicator.
  3. 3. The benzenesulfonic acid antibacterial film preparation according to claim 2, wherein the gel matrix and the stabilizer are selected from one of polyethylenimine crosslinked gel, polydiallyldimethyl ammonium chloride-based strong cationic polyelectrolyte, porous crosslinked polystyrene, ultra-high molecular weight polyethylene porous gel network, polyacrylamide crosslinked gel, anion exchange resin, colloidal silica, and/or, The color-developing indicator is a dye acceptable in pharmacy or food grade, and is selected from one of methylene blue, patent blue V, food pigment, methyl violet, bromocresol purple, bromophenol blue and carmine and/or, The PH of the benzenesulfonic acid anti-biofilm preparation is 2.0-5.0.
  4. 4. The benzenesulfonic acid anti-biofilm preparation according to claim 1, which consists of 10% -75% benzenesulfonic acid, 10% -40% propylene glycol, 5% -25% gel matrix and stabilizer, 0.001% -0.2% color indicator and the balance of water in percentage by mass; The gel matrix and the stabilizer are colloidal silica and are selected from at least one of LUDEX series or AEROSIL series colloidal silica, and the color-developing indicator is a dye acceptable in pharmacy or food grade and is selected from one of bromocresol purple and bromophenol blue.
  5. 5. The benzenesulfonic acid biofilm-resistant preparation of claim 4, comprising 30% -60% of benzenesulfonic acid, 15% -30% of propylene glycol, 10% -20% of colloidal silica, 0.05% -0.2% of color-developing indicator and the balance of water, wherein the benzenesulfonic acid biofilm-resistant preparation has a pH of 2.0-4.0.
  6. 6. The benzenesulfonic acid biofilm-resistant preparation of claim 4, which is composed of 50% -60% of benzenesulfonic acid, 18% -25% of propylene glycol, 10% -15% of colloidal silicon dioxide, 0.05% -0.1% of color development indicator and the balance of water, wherein the colloidal silicon dioxide is LUDEX SM-30 dispersion liquid, the color development indicator is bromocresol purple solution, and the pH of the benzenesulfonic acid biofilm-resistant preparation is 2.0-2.4.
  7. 7. A method for preparing the benzenesulfonic acid antibacterial film preparation according to any one of claims 1 to 6, comprising the steps of: S1, dissolving benzenesulfonic acid in propylene glycol under the stirring condition to form a mixed solution; S2, slowly adding a gel matrix and a stabilizer into the mixed solution, and continuously homogenizing and stirring at a high speed to form uniform gel; S3, adding a color development indicator into the gel, supplementing with purified water, and uniformly stirring to obtain the benzenesulfonic acid anti-biofilm preparation.
  8. 8. A method for removing bacterial biofilm, which is characterized by comprising the step of contacting a benzenesulfonic acid anti-biofilm preparation with a bacterial biofilm by 10 s-10 min, wherein the benzenesulfonic acid anti-biofilm preparation is prepared by the preparation method according to any one of claims 1-6 or by the preparation method according to claim 7.
  9. 9. Use of a benzenesulfonic acid anti-biofilm formulation or a method for removing a bacterial biofilm, wherein the benzenesulfonic acid anti-biofilm formulation is as claimed in any one of claims 1 to 6 or is produced by the production method as claimed in claim 7, and the method for removing a bacterial biofilm is as claimed in claim 8.
  10. 10. The use according to claim 8, wherein the bacterial biofilm comprises a biofilm formed by gram positive bacteria and gram negative bacteria.

Description

Benzenesulfonic acid anti-biological film preparation and preparation method and application thereof Technical Field The invention belongs to the technical field of antibacterial materials, and particularly relates to a benzenesulfonic acid antibacterial film preparation and a preparation method and application thereof. Background Bacterial biofilm is a membranous compound formed by adsorbing bacteria on the surface of biological materials or the surface of organism tissues in the growth process to adapt to living environment and wrapping the bacteria by secreting extracellular matrixes such as polysaccharide, protein, nucleic acid and the like. The formation of the biological film can lead to the remarkable enhancement of the resistance of bacteria to antibiotics and a host immune system, is an important cause for causing the problems of chronic infection, medical instrument related infection, industrial pipeline biological pollution and the like, and brings great challenges to the fields of medical health, industrial production and the like. To address the challenges presented by biofilms, the prior art has developed a variety of intervention strategies including physical debridement, enzymatic techniques, antibacterial drug combinations, chemical debridement, and the like. The chemical debridement technology has certain application in clinical and industrial scenes by virtue of the advantages of simple operation, rapid action, wide applicability and the like, and the core principle is that the chemical reagent is used for destroying the extracellular polymer structure of the biological membrane, killing bacteria in the membrane or inhibiting the adhesion of the bacteria, so that the biological membrane is removed and stripped. Prior art solutions exist for chemical debridement using sulphonic acids. For example, DEBX (WO 2021148124A 1) uses ethanesulfonic acid or 1-propanesulfonic acid and is combined with Dimethylsulfoxide (DMSO) as proton acceptor. Although the method is effective, the short-chain sulfonic acid used by the method is extremely strong (pKa is about-1.3 to-0.86), has high potential irritation to normal tissues, is especially not suitable for biological membrane debridement of sensitive parts such as chronic wound surfaces, mucous membrane tissues and the like, and meanwhile, DMSO is used as a powerful transdermal absorption promoter, which can promote the penetration of sulfonic acid compounds into the biological membranes, but can also accelerate the absorption of other potential harmful substances, has disputed biological safety in long-term use, has unpleasant garlic odor and seriously influences the use experience. Therefore, there is an urgent need to develop a bacterial biofilm removal solution that can avoid the above-mentioned drawbacks, and has high removal efficiency, good biosafety, and wide application range. Disclosure of Invention Aiming at the problems of insufficient efficacy, poor solvent applicability, narrow application range and the like of the traditional anti-biofilm preparation, the invention provides a benzenesulfonic acid anti-biofilm preparation containing benzenesulfonic acid and propanediol combination, and a preparation method and application thereof, which are clear of high-efficiency active ingredients and optimal preparation conditions, realize efficient removal of various bacterial biofilms, ensure high-efficiency debridement capability and simultaneously remarkably improve the safety and reliability of the preparation. In order to achieve the above purpose, the invention adopts the following technical scheme: the first aspect of the invention provides a benzenesulfonic acid anti-biological film preparation, which comprises, by mass, 10% -75% of benzenesulfonic acid and 10% -40% of propylene glycol. The benzenesulfonic acid can be used as an active ingredient to realize excellent biological film removal effect in a limited concentration range, and the effect is superior to that of methanesulfonic acid or ethanesulfonic acid with higher concentration, and the irritation to normal tissues is obviously reduced. The propylene glycol is used as an organic solvent and a permeation promoter, not only can effectively dissolve the benzenesulfonic acid, but also can generate synergistic effect with the benzenesulfonic acid by the osmotic pressure effect of the propylene glycol, and finally, the cleaning effect of the propylene glycol and the benzenesulfonic acid on the biological membrane by the combination of the benzenesulfonic acid and the benzenesulfonic acid is superior to that of a preparation taking DMSO as a solvent. Further, the benzenesulfonic acid anti-biological film preparation also comprises 5 to 25 percent of gel matrix, a stabilizer and 0.001 to 0.2 percent of color development indicator. Further, the gel matrix and the stabilizer are selected from Polyethylenimine (PEI) crosslinked gel, polydiallyl dimethyl ammonium chloride (PDADMAC) strong cationi