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CN-122004227-A - PH response type hexaconazole controlled release nano-particle and preparation method and application thereof

CN122004227ACN 122004227 ACN122004227 ACN 122004227ACN-122004227-A

Abstract

The invention discloses pH response hexaconazole controlled release nano-particles, a preparation method and application thereof, and belongs to the technical field of agricultural pesticide preparations and nano-materials. The invention solves the problems of poor water solubility, fast photolysis, low foliar adhesion rate, insufficient utilization rate and the like of the prior hexaconazole. According to the invention, molybdenum-based multi-acid nano-cage (MO) is used as a carrier, and hexaconazole (Hex) serving as a bactericide is loaded by an impregnation method, so that Hex@MO nano-particles are prepared. The prepared Hex@MO nano-particles have small particle size and good dispersibility, can remarkably improve the water solubility and the heat stability of Hex, have excellent leaf surface spreadability, are favorable for reducing the drift loss of pesticide application, have unique pH response release characteristics, can intelligently release pesticide in an acidic microenvironment generated by pathogen infection, and realize targeted delivery, thereby improving the sterilization efficiency, enhancing the sterilization activity, reducing the influence on non-target organisms and reducing the environmental risk.

Inventors

  • GAO SHUANG
  • LI FENGRUI
  • LI MEIQING
  • LIU YANYAN
  • REN HAO

Assignees

  • 东北农业大学

Dates

Publication Date
20260512
Application Date
20260126

Claims (10)

  1. 1. The pH response type hexaconazole controlled release nano-particle is characterized by comprising a polyacid nano-cage carrier and hexaconazole loaded by an impregnation method.
  2. 2. A method for preparing the pH-responsive hexaconazole controlled-release nanoparticle according to claim 1, comprising: (1) Adding ammonium acetate and diamine sulfate into the ammonium molybdate solution, uniformly stirring, slowly dropwise adding an acetic acid aqueous solution, standing at room temperature, filtering, washing and drying to obtain a polyacid nanocage carrier; (2) Dissolving hexaconazole in methanol, dissolving a polyacid nano cage carrier in water, slowly dripping a methanol solution of hexaconazole into the polyacid nano cage carrier solution, stirring at room temperature, centrifuging, washing and drying to obtain pH response type hexaconazole controlled release nano particles which are named as Hex@MO nano particles.
  3. 3. The method according to claim 2, wherein the amount of the ammonium molybdate, ammonium acetate and diamine sulfate in (1) is 1:36:1.36.
  4. 4. The process according to claim 2, wherein the aqueous acetic acid solution in (1) has a volume concentration of 50%.
  5. 5. The method according to claim 2, wherein the standing time in (1) is 7d.
  6. 6. The method according to claim 2, wherein (1) is washed with 90% ethanol and absolute ethanol in this order.
  7. 7. The process of claim 2, wherein the stirring time at room temperature in (2) is 24 hours.
  8. 8. The process according to claim 2, wherein the washing is performed with methanol in (2).
  9. 9. The method according to claim 2, wherein the drying in (2) is natural drying at room temperature.
  10. 10. The use of the pH-responsive hexaconazole controlled-release nanoparticle according to claim 1, wherein said nanoparticle is used as a controlled-release nano-pesticide for controlling rice sheath blight.

Description

PH response type hexaconazole controlled release nano-particle and preparation method and application thereof Technical Field The invention relates to pH response hexaconazole controlled release nano-particles, a preparation method and application thereof, and belongs to the technical field of agricultural pesticide preparations and nano-materials. Background Rice sheath blight is an important fungal disease caused by rhizoctonia solani (Rhizoctonia solani), and seriously threatens the yield of rice and the grain safety. At present, chemical bactericides such as hexaconazole (Hex) are mainly relied on for prevention and treatment, but the problems of poor water solubility, fast photolysis, low foliar adhesion rate, insufficient utilization rate and the like exist in practical application, so that the problems of frequent pesticide application, environmental pollution, high pesticide residue risk and the like are caused. The nano medicine carrying technology provides a new way for improving the utilization rate of pesticides and realizing intelligent controlled release. The polyoxometallate (polyacid) is an inorganic metal oxygen cluster formed by front transition metal, has the characteristics of adjustable structure, large specific surface area, good biocompatibility, excellent thermal performance and the like, and is an ideal pesticide carrier material. However, the application research of polyacid nanocages in hexaconazole pesticide delivery is less at present, and particularly an intelligent controlled release system aiming at rice sheath blight is not reported in a system. Disclosure of Invention The invention provides a pH response type hexaconazole controlled release nanoparticle and a preparation method and application thereof, and aims to solve the problems of poor water solubility, fast photolysis, low foliar adhesion rate, insufficient utilization rate and the like of the existing hexaconazole. The technical scheme of the invention is as follows: One of the purposes of the invention is to provide a pH response type hexaconazole controlled release nanoparticle, which comprises a polyacid nanocage carrier and hexaconazole loaded by an impregnation method. The second object of the present invention is to provide a method for preparing the pH-responsive hexaconazole controlled-release nanoparticle, which comprises the following steps: (1) Adding ammonium acetate and diamine sulfate into the ammonium molybdate solution, uniformly stirring, slowly dropwise adding an acetic acid aqueous solution, standing at room temperature, filtering, washing and drying to obtain a polyacid nanocage carrier; (2) Dissolving hexaconazole in methanol, dissolving a polyacid nano cage carrier in water, slowly dripping a methanol solution of hexaconazole into the polyacid nano cage carrier solution, stirring at room temperature, centrifuging, washing and drying to obtain pH response type hexaconazole controlled release nano particles which are named as Hex@MO nano particles. Further defined, the ratio of the amounts of the ammonium molybdate, ammonium acetate, and diamine sulfate materials in (1) is 1:36:1.36. Further defined, the aqueous acetic acid solution in (1) has a volume concentration of 50%. Further defined is (1) a rest time of 7d. Further defined, the washing is performed with ethanol having a concentration of 90% and absolute ethanol in this order in (1). Further defined, the stirring time at room temperature in (2) is 24 hours. Further defined, the washing is performed in (2) using methanol. Further defined, the drying in (2) is natural drying at room temperature. The invention further aims to provide an application of the pH responsive hexaconazole controlled release nano-particles, and the pH responsive hexaconazole controlled release nano-particles are particularly used as controlled release nano-pesticides for preventing and treating rice sheath blight. The beneficial effects are that: According to the invention, molybdenum-based multi-acid nano-cage (MO) is used as a carrier, and hexaconazole (Hex) serving as a bactericide is loaded by an in-situ encapsulation technology, so that hex@MO nano-particles are prepared. The prepared Hex@MO nano-particles have small particle size and good dispersibility, can remarkably improve the water solubility and the heat stability of Hex, have excellent leaf surface spreadability, are favorable for reducing the drift loss of pesticide application, have unique pH response release characteristics, can intelligently release pesticide in an acidic microenvironment generated by pathogen infection, and realize targeted delivery, thereby improving the sterilization efficiency, enhancing the sterilization activity, reducing the influence on non-target organisms and reducing the environmental risk. In addition, the carrier MO is safe to rice, can bidirectionally transport in plants and pathogenic bacteria, further improves the bioavailability of pesticides, provides a new idea for