CN-122004240-A - Application of butyl hydroxy toluene in enhancing biocontrol activity of entomopathogenic nematodes

Abstract

The invention discloses application of butyl hydroxy toluene in enhancing biocontrol activity of entomopathogenic nematodes. The activity of the entomopathogenic nematodes can be obviously enhanced by pretreating the entomopathogenic nematodes by using the volatile butyl hydroxytoluene specifically released by the red palm weevil larvae, so that the nematodes are quantitatively enhanced in behavior, the parasitic killing capacity of the entomopathogenic nematodes on hosts (larvae of red palm weevil, chilo suppressalis, tomato leaf miner and the like) is improved, and the control effect on various pests is effectively improved. And the butyl hydroxy toluene has no toxic or side effect on the entomopathogenic nematodes under the effective concentration, and ensures the survival and the stable activity of the entomopathogenic nematodes. The invention provides a safe, efficient and wide-application-range strategy for improving biological control means based on entomopathogenic nematodes, and has good popularization and application prospects.

Inventors

• WU SHENGYAN
• WU RUI
• Ma Anhai
• CHEN YIJING
• DENG HONGHONG

Assignees

• 福建农林大学

Dates

Publication Date

20260512

Application Date

20260129

Claims (10)

1. 1. The application of butyl hydroxy toluene in preparing insect pathogenic nematode attractant.
2. 2. The method of claim 1, wherein the entomopathogenic nematodes are Heterodera minor.
3. 3. The method according to claim 1, wherein the butylhydroxytoluene is used at a concentration of 2 ng/. Mu.L to 2000 ng/. Mu.L.
4. 4. The application of butyl hydroxy toluene in preparing products for enhancing biocontrol activity of entomopathogenic nematodes.
5. 5. The method according to claim 4, wherein the biocontrol activity is the activity of entomopathogenic nematodes and the mortality of pests.
6. 6. The method of claim 4, wherein the entomopathogenic nematodes are Heterodera minor.
7. 7. The method according to claim 4, wherein the butylhydroxytoluene is used at a concentration of 2 ng/. Mu.L to 2000 ng/. Mu.L.
8. 8. The method according to claim 5, wherein the pest is red palm weevil, chilo suppressalis or tomato leaf miner.
9. 9. A biocontrol active product for improving entomopathogenic nematodes is characterized by comprising butyl hydroxytoluene, wherein the entomopathogenic nematodes are strongylus plutella xylostella.
10. 10. A method for improving the biocontrol activity of entomopathogenic nematodes is characterized by placing the entomopathogenic nematodes in 2 ng/mu L-2000 ng/mu L of aqueous solution of butylhydroxytoluene for 24-72 h to improve the activity of the entomopathogenic nematodes and the mortality of pests, wherein the entomopathogenic nematodes are Heterodera parvifolia, and the pests are red palm weevil, chilo suppressalis and tomato leaf miners.

Description

Application of butyl hydroxy toluene in enhancing biocontrol activity of entomopathogenic nematodes Technical Field The invention relates to the fields of biological control and agricultural pest control, in particular to application of butyl hydroxy toluene in enhancing the biological control activity of entomopathogenic nematodes. Background Entomopathogenic nematodes, such as the moth, s.pluvialis (STEINERNEMA CARPOCAPSAE), are recognized as effective biological control agents for a variety of insect pests due to their active search for hosts, efficient infection mechanisms, and safety to humans, animals and the environment. They find wide application in the control of agricultural and forestry pests, and in particular show great potential in the control of soil insects and cryptic herbivorous insects. However, despite the advantages of entomopathogenic nematodes, they still have limitations in locating and infecting cryptic insect hosts living in plant tissues or cryptic environments. These cryptic environments reduce the chemical signals available to nematodes, complicating host detection and thus reducing infection rates. Recent studies have shown that the host source of nematodes Volatile Organic Compounds (VOCs) can profoundly influence their behavior. The specific volatile compounds released by the host larvae can serve as attractants and behavioral promoters to enhance nematode positioning and infection efficiency. Thus, looking for specific chemical signals for these hosts, it is expected to overcome the natural limitations of cryptic pest host detection, thereby improving the practical effectiveness of nematode-based biocontrol. Disclosure of Invention The invention aims to provide an application of butyl hydroxy toluene in enhancing the biocontrol activity of entomopathogenic nematodes. The motor and lethal host capacities of entomopathogenic nematodes are enhanced by the use of the host source volatile butyl hydroxytoluene (butylated hydroxytoluene, BHT). In order to achieve the above purpose, the present invention adopts the following technical scheme: in a first aspect, the invention provides the use of butylated hydroxytoluene in the preparation of an insect-pathogenic nematode attractant. Further, the entomopathogenic nematodes are strongylus exigua. Further, the using concentration of the butyl hydroxy toluene is 2 ng/mu L-2000 ng/mu L. In a second aspect, the invention provides the use of butylhydroxytoluene in the preparation of a product for enhancing the biocontrol activity of entomopathogenic nematodes. Further, the biocontrol activity is the activity ability of entomopathogenic nematodes and the mortality rate to pests, which are red palm weevil, chilo suppressalis and tomato leaf miner. Further, the entomopathogenic nematodes are strongylus exigua. Further, the using concentration of the butyl hydroxy toluene is 2 ng/mu L-2000 ng/mu L. In a third aspect, the invention provides a biocontrol active product comprising butylhydroxytoluene for increasing the activity of an entomopathogenic nematode, which is a strongylus exigua. In a fourth aspect, the invention provides a method for improving the biocontrol activity of entomopathogenic nematodes, which comprises the steps of placing entomopathogenic nematodes in 2 ng/. Mu.L-2000 ng/. Mu.L of aqueous solution of butylhydroxytoluene for 24-72 hours to improve the activity of the entomopathogenic nematodes and the mortality of the entomopathogenic nematodes to pests, wherein the entomopathogenic nematodes are cabbage caterpillar, and the pests are red palm weevil, chilo suppressalis and tomato leaf miner. Compared with the prior art, the invention has the following remarkable advantages: (1) And the pest mortality rate is enhanced, namely, the locomotor activity and pathogenicity of nematodes pretreated by or used with BHT are improved, and the mortality rate of target pests can be obviously improved. (2) No toxic side effects on nematodes BHT has no adverse effect on survival and physiological health of the entomopathogenic nematodes at BHT concentrations up to 2000 ng/μl, and no increase in nematode mortality is observed even over exposure periods up to 16 days. This ensures the safety of the biocontrol agent and avoids potential harm to the environment and non-target organisms. (3) The application is wide, although the BHT is a volatile matter specifically released by the red palm weevil, exogenous application of the BHT to other pests (such as Chilo suppressalis and tomato leaf miner) can also effectively improve the attraction and killing capacity of nematodes. This means that the method can be popularized and applied to various hidden or non-hidden pests which do not naturally produce BHT, and expands the biological control pedigree of the entomopathogenic nematodes. (4) Is easy to integrate into the existing biological control preparation, and in view of the specificity, safety and behavior activation effect of BHT, the invention p