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CN-122004241-A - Methylobacillus strains for improving plant production and quality and related methods

CN122004241ACN 122004241 ACN122004241 ACN 122004241ACN-122004241-A

Abstract

The present application relates to a strain of methylobacterium for improving plant production and quality and related methods. Provided herein are methylobacterium strains (Methylobacterium strain) that enhance early plant growth and methods of use thereof. Methods for identifying methylobacterium strains that can be used to increase the content of one or more mineral nutrients and/or vitamins in green leaf plants are also provided. Also provided are related methods of providing green leaf plants having increased levels of one or more mineral nutrients and/or vitamins, as well as green leaf plants having increased levels of one or more mineral nutrients and/or vitamins and green leaf articles harvested from the plants, as a result of treatment with a methylobacterium strain as provided herein.

Inventors

  • Patrick Wogan
  • Jenny Clovis
  • Natalie Brackfield

Assignees

  • 新叶共生有限公司

Dates

Publication Date
20260512
Application Date
20210602
Priority Date
20200602

Claims (20)

  1. 1. A method for enhancing plant growth and/or cannabis plant rooting, the method comprising: (a) Treating cannabis plants, plant parts or seeds with a composition comprising one or more methylobacterium isolates, and (B) Growing the treated plant or growing a plant from the treated plant part or seed to produce a rooted plant, wherein cannabis plant growth and/or rooting is increased compared to an untreated control plant that has not received application of the composition or compared to a control plant grown from an untreated plant part or seed that has not received application of the composition.
  2. 2. The method of claim 1, wherein the composition comprises a methylobacterium isolate selected from the group consisting of LGP2002 (NRRL B-50931), LGP2009 (NRRL B-50938), and LGP2019 (NRRL B-67743).
  3. 3. The method of claim 1 or 2, wherein the plant part is derived from cutting of a cannabis plant.
  4. 4. A method according to claim 3, wherein the cuttings are treated by immersion in a methylobacterium suspension.
  5. 5. The method of claim 4, wherein the methylobacterium is present in the suspension at a concentration of greater than 1 x 10 3 CFU per milliliter.
  6. 6. The method of claim 1 or 2, wherein the rooted plants are transplanted to a field, and wherein the production cycle time of mature cannabis plants is reduced compared to control cannabis plants grown from untreated cuttings.
  7. 7. The method of claim 1 or 2, wherein the enhanced growth results in an improvement in a plant trait of the plant selected from the group consisting of increased biomass production, reduced cycle time, increased leaf growth rate, increased root growth rate, and increased seed yield.
  8. 8. The method of claim 1 or 2, wherein the composition further comprises at least one additional component selected from the group consisting of additional active ingredients, agriculturally acceptable adjuvants, and agriculturally acceptable excipients.
  9. 9. Use of a composition comprising a methylobacterium isolate, the cannabis plant, part or seed being at least partially coated with a composition comprising the methylobacterium isolate, the methylobacterium isolate being selected from the group consisting of LGP2002 (NRRL B-50931), LGP2009 (NRRL B-50938) and LGP2019 (NRRL B-67743), wherein the cannabis plant or cannabis plant grown from a part or seed of a control cannabis plant not treated with the methylobacterium exhibits enhanced plant growth or rooting or reduced cycle time from cutting to mature plants compared to a cannabis plant not treated with the methylobacterium or a cannabis plant grown from a part or seed of a control cannabis plant not treated with the methylobacterium.
  10. 10. Use of methylobacterium in the treatment of a green leaf plant or plant part, wherein the green leaf plant or plant part has increased levels of one or more mineral nutrients and/or vitamins, wherein the plant or plant part is harvested from a cultivated plant grown from a methylobacterium treated seed or seedling, and wherein the methylobacterium comprises LGP2009 (NRRL B-50938) and provides increased levels of one or more mineral nutrients and/or vitamins.
  11. 11. The green leaf plant or plant part according to claim 10, wherein the cultivated plant is selected from the group consisting of spinach, lettuce, beet, swiss beet, watercress, collard, cabbage mustard, endive, sesame seed, chicory, chinese cabbage and turnip, spinach plant or plant part.
  12. 12. The use according to claim 10 or 11, wherein the plant or plant part has increased levels of one or more mineral nutrients selected from the group consisting of nitrogen, magnesium and iron.
  13. 13. A method for identifying a methylobacterium isolate that increases the content of one or more mineral nutrients and/or vitamins in a green leaf plant, the method comprising: (i) Treating a green leaf plant seed or green leaf plant seedling with at least a first methylobacterium isolate; (ii) Cultivating the seed or seedling to obtain a treated plant having at least two true leaves; (iii) Harvesting the plant, plant shoot, or one or more true leaves from the treated plant; (iv) Analyzing the harvested plants, shoots or plant leaves to determine mineral nutrient and/or vitamin content, and (V) A methylobacterium isolate is selected that increases the content of at least one mineral nutrient or vitamin in the treated plant, plant shoot, or plant leaf compared to a plant, plant shoot, or plant leaf harvested from an untreated control plant, or a plant, plant shoot, or plant leaf harvested from a plant treated with a second methylobacterium isolate.
  14. 14. The method of claim 13, wherein the seed and/or plant is treated with two or more different methylobacterium isolates, respectively, in step (i), and cultivated, harvested and analyzed, respectively, in steps (ii) to (iv), to determine mineral nutrient and/or vitamin content in the plant, shoot or leaf or leaves of the plant, as compared to other methylobacterium isolates and optionally to untreated control plants.
  15. 15. The method of claim 13, wherein the seed and/or plant is treated in step (i) with three or more different methylobacterium strains or a combination of different methylobacterium strains, respectively, as compared to other different methylobacterium treatments and optionally to untreated control plants, and the treated seed or seedling is cultivated, harvested and analyzed in steps (ii) to (iv), respectively, to determine the mineral nutrient and/or vitamin content in the plant, shoot or leaf or leaves of the plant.
  16. 16. The method of any one of claims 13 to 15, wherein one or more of the different methylobacterium strains has enhanced colonization efficiency.
  17. 17. The method of any one of claims 13 to 15, wherein the green leaf plant is cultivated in a hydroponic system or an aeroponic system.
  18. 18. The method according to any one of claims 13 to 15, wherein the methylobacterium strain selected in (v) also imparts an improvement in a trait selected from the group consisting of increased biomass production, reduced cycle time, increased leaf growth rate, reduced time to two true leaves, increased root growth rate and increased seed yield to the green leaf plant due to increased content of at least one mineral nutrient or vitamin.
  19. 19. The method according to any one of claims 13 to 15, wherein the green leaf plant is selected from the group consisting of spinach, lettuce, beet, swiss beet, watercress, kale, cabbage mustard, endive, sesame, chicory, chinese cabbage and turnip.
  20. 20. The method according to any one of claims 13 to 15, wherein the green leaf plant is cultivated for the production of miniature green leaf vegetables and/or herbs.

Description

Methylobacillus strains for improving plant production and quality and related methods The present application is a divisional application of application No. 202180043537.4, having application No. 2021, 6/2, and entitled "Methylobacillus strain and related methods for improving plant production and quality". Cross reference to related applications The international patent application claims the benefit of U.S. provisional patent application No. 63/033,364, filed on month 6 and 2 of 2020, and U.S. provisional patent application No. 63/088,837, filed on month 10 and 7 of 2020, the entire disclosures of which are incorporated herein by reference. Statement of sequence Listing The sequence listing contained in the file named "LEAFYGREENS _st25.txt" having a size of 22,944 bytes (measured in MS-Windows) and created at 5.25 of 2021 contains 76 nucleic acid sequences provided herewith by the EFS system of USPTO, and is incorporated herein by reference in its entirety. Background Plants require certain macronutrients and micronutrients for growth and metabolism. These elements are typically present in the soil as salts and can be absorbed by plants as ions. In agriculture, soil may be depleted of one or more of these nutrients, requiring the addition of fertilizers to provide sufficient amounts of nutrients for crop growth. In hydroponic systems, all nutrients must be supplied to the growing plants and are typically the largest cost of hydroponic plant production systems. Plants are an important part of a healthy diet and in particular high levels of vitamins and nutrients for green leaf vegetables are known. Methods to increase yield by improving plant growth and/or increasing macronutrient and micronutrient levels are desired to benefit agricultural practices as well as human and animal nutrition. One-carbon type organic compounds (one-carbon organic compounds) such as methane and methanol are widely found in nature, and bacteria classified as methane-oxidizing bacteria and methylotrophic bacteria are used as carbon sources. The methane-oxidizing bacteria (Methanotrophic bacteria) include species of genus Methylobacillus (Methylobacter), methylomonas (Methylomonas), methylococcus (Methylomicrobium), methylococcus (Methylococcus), methylotrichum (Methylosinus), methanocyaneus (Methylocystis), methylococcus (Methylosphaera), methanothermus (Methylocaldum), and Methylocyte (Methylocella) (Lidstrom, 2006). The methane oxidizing bacteria possess a methane monooxygenase enzyme that incorporates oxygen atoms in O 2 into methane to form methanol. All methane-oxidizing bacteria are obligate one-carbon users (one-carbon utilizers) who cannot use compounds containing carbon-carbon bonds. On the other hand, methylotrophic bacteria can also utilize more complex organic compounds such as organic acids, higher alcohols, sugars, etc. Thus, methylotrophic bacteria are facultative methylotrophic bacteria. the methylotrophic bacteria include Methylobacillus (Methylobacterium), hyphomicrozyme (Hyphomicrobium), methylophilus (Methylophilus), methylobacillus (Methylobacillus), methylophaga (Methylophaga), aminobacillus (Aminobacter), methylobacillus (Methylorhabdus), methylococcus (Methylopila), methylsulfonyl (Methylosulfonomonas), Sulfonyl Marshall (Marinosulfonomonas), paracoccus (Paracoccus), flavobacterium (Xanthobacter), flexibacterium (Ancylobacter) (also known as Proteus (Microcyclus)), thiobacillus, rhodopseudomonas, rhodobacillus (Rhodobacter), acetobacter (Acetobacter), bacillus (Bacillus), pyriculatus (Rhodopseudomonas), rhodopseudomonas (Rhodobacter, and (Rhodobacter) may be used as a source of bacteria, Genus species of mycobacteria (Mycobacterium), arthrobacter (Arthobacter) and Nocardia (Nocardia) (Lidstrom, 2006). Some methylotrophic bacteria of the genus methylobacterium appear pink. They are conventionally known as PPFM bacteria and are pink facultative methylotrophic bacteria. Green (2005, 2006) identified twelve confirmed species among Methylobacillus genus, specifically, methylobacillus aminophilus (M. aminovorans), methylobacillus chloromethane (M. chloromethanicum), methylobacillus dichlormethane (M. dichloromethanicum), methylobacillus torvus (M. extorquens), methylobacillus tenuifidus (M. fujisawaense), methylobacillus mesophilic (M. mesophilicum), methylobacillus organophilus (M. organophilum), methylobacillus radiodurans (M. radiotolerans), methylobacillus roteins (M. rhodesianum), methylobacillus roseus (M. rhodinum), methylobacillus thiorensis (M. thiocyanatum), and Methylobacillus zakii (M. zatmanii). However, methylobacillus nodulation (M. nodulans) is a non-PPFM genus of azotobacter (Sy et al, 2001). Methylobacterium is present in soil, dust, fresh water, sediment and leaf surfaces, as well as in industrial and clinical environments (Green, 2006). Disclosure of Invention Provided herein are compositions comprising one or more methylobacterium strains that enhance early growth, increase reprod