CN-122004243-A - Microbial agent for improving yield and quality of strawberries as well as preparation method and application of microbial agent

CN122004243ACN 122004243 ACN122004243 ACN 122004243ACN-122004243-A

Abstract

The invention discloses a microbial agent for improving yield and quality of strawberries, and a preparation method and application thereof, and belongs to the technical field of microorganisms. The microbial agent comprises, by weight, 40-50 parts of a composite microbial agent, 0.3-0.5 part of xanthan gum, 1.5-2 parts of glycerol, 4-5 parts of a citric acid-sodium citrate buffer solution, 1-1.5 parts of potassium humate, 0.3-0.5 part of monopotassium phosphate and 0.2-0.3 part of chelated calcium and magnesium, and water to 100 parts, wherein the composite microbial agent comprises Arthrobacter sinkianus, listeria filiformis and Bacillus coagulans. The microbial agent can effectively prevent and treat strawberry diseases, including root rot, fusarium wilt, gray mold and anthracnose, comprehensively promote root development of strawberry plants, nutrient absorption and strawberry growth through synergistic effects of hormone secretion, nutrient activation, disease inhibition and the like, remarkably improve strawberry yield and fruit quality, and remarkably reduce strawberry storage decay rate.

Inventors

ZHOU YIXIN
WU HAN
Jiao Yineng
CHEN WANYANG
HUANG JIE
FAN DENG
LIU ZIRONG
ZHANG XIAOYANG
LIU XIAODAN
LIU SHENGWU

Assignees

湖北茂盛生物有限公司

Dates

Publication Date: 20260512
Application Date: 20260130

Claims (8)

1. The microbial agent for improving the yield and quality of the strawberries is characterized by comprising, by weight, 40-50 parts of a composite microbial agent, 0.3-0.5 part of xanthan gum, 1.5-2 parts of glycerol, 4-5 parts of a citric acid-sodium citrate buffer solution, 1-1.5 parts of potassium humate, 0.3-0.5 part of monopotassium phosphate, 0.2-0.3 part of chelated calcium magnesium and water, wherein the water is added to 100 parts.
2. The microbial agent for improving the yield and quality of strawberries according to claim 1, wherein the compound microbial agent comprises Arthrobacter sinkiangensis, listeria lineans and bacillus coagulans.
3. The microbial agent for improving the yield and quality of strawberries according to claim 2, wherein the compound microbial agent comprises a strain of Arthrobacter sinkiangensis with a preservation number of CGMCC 1.8987, a strain of Listeria filiformis with a preservation number of CGMCC 1.8801 and a strain of Bacillus coagulans with a preservation number of CGMCC1.10823.
4. The microbial agent for improving the yield and quality of strawberries according to claim 1, wherein the preparation method of the citric acid-sodium citrate buffer solution is as follows: Preparing 0.1mol/L citric acid solution and 0.1mol/L sodium citrate solution respectively by deionized water, uniformly mixing the citric acid solution and the sodium citrate solution according to a volume ratio of 1:4, regulating the pH value to 6.5, sterilizing the prepared mixed solution by high-pressure steam at 121 ℃ for 20min, and cooling for later use.
5. The microbial agent for improving the yield and the quality of strawberries according to claim 1, wherein the preparation method of the compound microbial agent is as follows: (1) Activating the linear listeria in a nutrient gravy agar culture medium, picking single bacterial colonies, inoculating the single bacterial colonies into the nutrient gravy liquid culture medium, culturing at 30 ℃ and 170r/min until OD 600 = 0.6 to obtain seed liquid, inoculating the seed liquid into the nutrient gravy liquid culture medium of a fermentation tank according to the amount of 5%, culturing at 30 ℃ and 170r/min until the viable count reaches 1× 9 cfu/mL, and ending culturing to obtain linear listeria fermentation liquor; (2) Activating Arthrobacter sinkiangensis in an LB solid culture medium, picking single bacterial colony, inoculating the single bacterial colony into the LB liquid culture medium, culturing at 20 ℃ and 210r/min until OD 600 = 0.6 to obtain seed liquid, inoculating the seed liquid into the LB liquid culture medium of a fermentation tank according to the quantity of 5%, culturing at 20 ℃ and 210r/min, and ending culturing after the number of detected viable bacteria reaches 1X 10 9 cfu/mL to obtain Arthrobacter sinkiangensis fermentation liquor; (3) Activating bacillus coagulans in an MRS (minimum shift register) culture medium, picking single bacterial colonies, inoculating the single bacterial colonies into the MRS liquid culture medium, culturing at 30 ℃ and 200r/min until OD 600 = 0.6 to obtain seed liquid, inoculating the seed liquid into the MRS liquid culture medium of a fermentation tank according to the amount of 1% for culturing at 30 ℃ and 200r/min, and ending culturing after the number of detected viable bacteria reaches 1X 10 9 cfu/mL to obtain bacillus coagulans fermentation liquor; (4) Mixing the prepared linear listeria fermentation liquor, the Arthrobacter sinkiang fermentation liquor and the bacillus coagulans fermentation liquor according to the volume ratio of 3:4:3 to obtain the composite microbial agent.
6. A method for preparing the microbial agent for improving the yield and quality of strawberries according to claim 1, which is characterized by comprising the following steps: Firstly preparing a composite microbial agent and a citric acid-sodium citrate buffer solution, putting the prepared water into a stirring container, sequentially adding xanthan gum, glycerol and the citric acid-sodium citrate buffer solution, starting a stirring device, stirring until all components are completely dissolved, continuously adding potassium humate, monopotassium phosphate and chelated calcium and magnesium into the container, keeping a stirring state until all solid components are completely dispersed and dissolved, and finally adding the prepared composite microbial agent, and stirring uniformly to obtain the microbial agent for improving the yield and quality of strawberries.
7. The use of the microbial agent for improving the yield and the quality of strawberries according to claim 1, wherein the microbial agent is used for preventing diseases and promoting the growth of strawberries, and the yield and the quality of strawberries are remarkably improved.
8. The use according to claim 7, characterized in that it is effective for controlling strawberry root rot, strawberry anthracnose, strawberry gray mold and strawberry blight.

Description

Microbial agent for improving yield and quality of strawberries as well as preparation method and application of microbial agent Technical Field The invention belongs to the technical field of microorganisms, and particularly relates to a microbial agent for improving yield and quality of strawberries, and a preparation method and application thereof. Background Strawberry is originally produced from south america, belongs to rosaceous herbaceous plants, is respectively called pineapple strawberry, raspberry, strawberry and the like, has delicious pulp and good taste, contains abundant nutrient substances and physiological active ingredients, and is known as fruit queen by people. In recent years, the strawberry industry rapidly develops, and the cultivation mode mainly comprises facility cultivation, and adopts cultivation modes such as sunlight greenhouse, multilayer film coverage, winter greenhouse, bow greenhouse and the like. In order to increase the yield of strawberries, many strawberries increase the application amount of chemical fertilizers year by year in the production process, and the soil hardening, the disease increase, the yield and quality reduction of strawberries and the like are caused by successive year rotation, and the method is mainly characterized by reduced fruit size, increased malformed fruits, reduced fruit uniformity, thick and loose meat quality, sour or tasteless taste, shorter shelf life, higher rotten fruit rate and the like, so that the economic benefit of strawberries is influenced, and the healthy development of the strawberry industry is restricted. In addition, the traditional planting relies on chemical pesticides and fertilizers, and pesticide residues are out of standard and harm to human health, which is contrary to the green agricultural development concept. Under the background, the microbial agent becomes an ideal choice for replacing chemical input products and optimizing strawberry planting modes due to the characteristics of environmental protection, high efficiency and sustainability, and has been widely focused in the fields of crop disease prevention and growth promotion. At present, the existing microbial agents for strawberry planting mainly comprise single strains, have single functions, and are difficult to realize the synergistic effect of disease comprehensive control, growth promotion and quality improvement. Although some composite bacterial agents try to combine different bacterial strains, the suitability among the bacterial strains is insufficient, a functional complementary synergistic mechanism is not formed, the defects of limited disease prevention range, unstable growth promotion effect, low nutrient activation efficiency and the like exist, the fruit storability is difficult to effectively improve, and the actual requirements of farmers on high-yield, high-quality and high-stress-resistance strawberry planting cannot be met. Therefore, aiming at the core pain point of strawberry planting, a composite microbial agent which can realize comprehensive disease prevention and control, efficient nutrient activation, growth promotion and quality improvement synergistic effect and is suitable for the growth characteristics of strawberries is developed, and has important practical significance for promoting the green sustainable development of the strawberry industry and improving the planting benefit. Disclosure of Invention The invention aims to provide a microbial agent for improving the yield and quality of strawberries, which can effectively improve the yield and quality of strawberries and improve the storability of strawberries. In order to achieve the technical purpose, the invention adopts the following technical scheme: The microbial agent for improving the yield and quality of the strawberries comprises, by weight, 40-50 parts of a composite microbial agent, 0.3-0.5 part of xanthan gum, 1.5-2 parts of glycerol, 4-5 parts of a citric acid-sodium citrate buffer solution, 1-1.5 parts of potassium humate, 0.3-0.5 part of monopotassium phosphate, 0.2-0.3 part of chelated calcium magnesium and 100 parts of water. Further, the compound microbial agent comprises Arthrobacter sinkiangensis, listeria linearly and Bacillus coagulans. Further, the compound microbial agent comprises Arthrobacter sinkiangensis purchased from China general microbiological culture collection center, wherein the strain preservation number is CGMCC 1.8987, the original preservation date is 3 months and 3 days in 2009, listeria filiformis is purchased from China general microbiological culture collection center, the strain preservation number is CGMCC 1.8801, the original preservation date is 21 months and 10 months in 2008, bacillus coagulans is purchased from China general microbiological culture collection center, the strain preservation number is CGMCC1.10823, and the original preservation date is 3 months and 3 days in 2010. Further, the preparation method of the citri