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CN-122004245-A - DSE chlamydospore aqua and preparation method and application thereof

CN122004245ACN 122004245 ACN122004245 ACN 122004245ACN-122004245-A

Abstract

The invention provides a DSE chlamydospore aqua and a preparation method and application thereof, belonging to the technical field of microbial preparations, and comprising chlamydospores from at least one dark-colored endophytic fungus, 5 g/mL-10 g/mL of preservative and 5 g/mL-10 g/mL of protective agent, wherein the content of chlamydospores is more than 2.5X10 8 CFU/mL. The invention improves the product property of the water aqua by reasonably optimizing the dosage of DSE chlamydospores and auxiliary materials, the chlamydospores content in the obtained water aqua finished product can reach 2.54 multiplied by 10 8 CFU/mL, each index accords with the national standard, the water aqua can be stored for more than 12 months at normal temperature, and the shelf life is prolonged by more than 9 months compared with the conventional conidium water aqua. The DSE chlamydospore aqua can effectively prevent and treat tomato bacterial wilt and southern rice black streaked dwarf, improve the growth vigor of tomato plants and rice plants, and can obtain the prevention and treatment effect which is not inferior to or even better than that of positive control trichoderma harzianum. The DSE chlamydospore aqua provided by the invention is simple to prepare, has controllable cost and excellent performance, and is suitable for large-scale popularization and use as a novel biological pesticide product.

Inventors

  • ZENG FENGHUA
  • ZHANG YAN
  • XIE LING
  • LONG YANYAN
  • ZHANG WENLONG
  • ZHAO HUILI

Assignees

  • 广西壮族自治区农业科学院

Dates

Publication Date
20260512
Application Date
20260410

Claims (10)

  1. 1. The DSE chlamydospore aqua is characterized by comprising chlamydospores from at least one dark-colored endophytic fungus, preservative of 0.5-2.5 mg/mL and protective agent of 0.5-1.0 mg/mL, wherein the content of chlamydospores in the DSE chlamydospore aqua is more than 2.5X10 8 CFU/mL.
  2. 2. The DSE chlamydospore aqua according to claim 1, characterized in that said dark-colored endophytic fungi comprise phinium, said phinium comprising a strain HX2 of phinium guangxiense (Cladophialophora guangxiense), with a preservation number of CGMCC No. 41498.
  3. 3. The DSE chlamydospore aqua according to claim 1, wherein the preservative is potassium sorbate, and the adding amount of the potassium sorbate in the DSE chlamydospore aqua is 0.5 mg/mL-1.0 mg/mL or 0.5 mg/mL-0.75 mg/mL or 0.5 mg/mL-0.6 mg/mL; The protective agent is dextrin, and the addition amount of the dextrin in the DSE chlamydospore water aqua is 0.5 mg/mL-1 mg/mL or 0.75 mg/mL-1.0 g/mL or 0.9 mg/mL-1.0 mg/mL.
  4. 4. A method for preparing a DSE chlamydospore aqua according to any one of claims 1 to 3, comprising the steps of: (1) Preparing chlamydospore pulp, namely culturing a dark-colored isolated endophytic fungus strain in a liquid basic culture medium, and collecting chlamydospores in fermentation liquor to obtain chlamydospore pulp; The method for preparing chlamydospore pulp comprises inoculating on PDA culture medium plate, performing activation culture at 28deg.C, transferring to basic culture medium, culturing in shaking table at 28deg.C for 14d, filtering the obtained chlamydospore fermentation broth with three layers of sterile gauze to obtain chlamydospore pulp; (2) And (3) preparing a finished product water aqua, namely adding a preservative and a protective agent into the chlamydospore pulp according to a proportion to obtain the finished product DSE chlamydospore water aqua.
  5. 5. Use of a DSE chlamydospore aqua according to any one of claims 1 to 3, for promoting the expression of plant defense enzymes, including PAL, PPO, POD, CAT and SOD.
  6. 6. The application of the DSE chlamydospore aqua in preventing and treating southern rice black streaked dwarf disease, which is characterized by comprising the step of diluting the DSE chlamydospore aqua to the concentration of chlamydospores of 1X 10 5 ~1×10 8 CFU/mL or 1X 10 5 ~1×10 6 CFU/mL.
  7. 7. The application of the DSE chlamydospore aqua in preventing and treating tomato bacterial wilt is characterized in that the DSE chlamydospore aqua is used by diluting the DSE chlamydospore aqua to the concentration of chlamydospores of 1X 10 5 ~1×10 7 CFU/mL or 1X 10 6 ~1×10 7 CFU/mL.
  8. 8. Use of DSE chlamydospores aqua according to any of claims 1 to 6, for promoting plant growth, said plants comprising at least one of rice and tomato.
  9. 9. The use according to claim 8, wherein the plant is tomato and the DSE chlamydospore aqua is applied by diluting the DSE chlamydospore aqua to a concentration of 1 x 10 5 ~1×10 7 CFU/mL or 1 x 10 6 ~1×10 7 CFU/mL chlamydospores.
  10. 10. The use according to claim 8, wherein the plant is rice and the DSE chlamydospore aqua is used by diluting the DSE chlamydospore aqua to a concentration of 1 x 10 5 ~1×10 8 CFU/mL or 1 x 10 5 ~1×10 6 CFU/mL chlamydospores.

Description

DSE chlamydospore aqua and preparation method and application thereof Technical Field The invention belongs to the technical field of microbial preparations, and particularly relates to a DSE chlamydospore aqua and a preparation method and application thereof. Background Tomato and rice are used as economic crops and grain crops which are important in China and even worldwide, and the yield and quality of the tomato and rice are directly related to the safety of agricultural production, the stable supply of grain and the economic benefits of peasants. However, in the intensive and large-scale planting mode, the frequency and spread of soil-borne diseases and virus diseases have become critical problems for restricting the quality and the high yield of two crops. Wherein, tomato bacterial wilt is caused by Ralstonia solanacearum (Ralstonia solanacearum), is a typical soil-borne vascular bundle disease, has the characteristics of high transmission speed, wide host range, high control difficulty and the like, and can cause rapid wilting and death of tomato plants after the disease, the incidence of common fields can reach 20% -30%, serious plots and even dead plants, and the serious economic loss is caused for the tomato industry. The southern rice black-streaked dwarf disease is a viral disease caused by southern rice black-streaked dwarf virus (Southern rice black-STREAKED DWARF virus, SRBSDV), is mainly transmitted by sogatella furcifera (Sogatella furcifera), and the diseased rice is characterized by dwarf plants, dark green leaves, strong stalks, no heading or unsmooth heading, and has been popular in the main production area of southern rice in China for multiple outbreaks in recent years, thus seriously threatening the rice yield and aggravating the pressure of grain production. At present, the biocontrol microorganism aiming at tomato bacterial wilt mainly uses bacillus, for example, bacillus subtilis SB10 bacterial body weight suspension and fermentation liquor separated from Liu Yumin and the like (2024) have preventive and therapeutic effects on bacterial wilt. Mekonnen et al (2022) found that bacillus isolate BDUA1 could significantly reduce the incidence of bacterial wilting of tomatoes under greenhouse conditions. The problems of weak colonization capability and unstable control effect of the biocontrol bacteria generally exist due to complex field soil environment and high differentiation of physiological race of the bacterial wilt bacteria. The prevention and treatment of southern rice black streaked dwarf mainly takes chemical agents such as imidacloprid, profenofos, pymetrozine and the like as main agents, and the research on biological agents is relatively less. Therefore, in order to find high-efficiency, safe and environment-friendly biological control resources, the development of novel biological pesticides has become a research hotspot and urgent need in the field of green prevention and control of agricultural diseases. The endophyte (DARK SEPTATE Endophytes, DSE) with dark color is a kind of endophyte group which is widely planted in the inside or on the surface of plant root system and forms dark color with hypha and microsclerotium, and can form symbiotic relation with various plants. Research shows that DSE can enhance the tolerance of plants to biotic and abiotic stress through the ways of promoting nutrient absorption, secreting growth regulating substances, inducing plant resistance, inhibiting pathogenic bacteria growth and the like, and has great application potential in biological control of crop diseases. The DSE belongs to a group with low abundance and high function, has deep color, can penetrate and colonize in plant tissues by hyphae and microsclerotium, has the advantages of strong colonization force, lasting activity and broad resistance spectrum, and has wide development and utilization prospects. In the prior studies, for the use of fungi, mycelium is often inoculated directly into plants or conidia are used for preparing a microbial inoculum. However, mycelia are easy to deactivate in the practical application process, the shelf life is very short (only 3-6 months), commercial requirements are hardly met, the shelf life of the conventional conidiophore fungicide is also limited, and large-scale application is difficult. The chlamydospores of fungi become the first choice ingredient for the development of the microbial inoculum because of the advantages of strong stress resistance and stability, and when the inventor tries to prepare the aqueous agent by using the spores, the inventor finds that the spores have the problems that the germination rate is low or the effective viable bacteria number is low, so that the effects are difficult to play, and great challenges are brought to the product development of DSE. Disclosure of Invention In order to solve the technical problems, the invention provides a DSE chlamydospore aqua and a preparation method and application th