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CN-122004246-A - DSE chlamydospore wettable powder and preparation method and application thereof

CN122004246ACN 122004246 ACN122004246 ACN 122004246ACN-122004246-A

Abstract

The invention provides DSE chlamydospore wettable powder, a preparation method and application thereof, belonging to the technical field of microbial preparations, and comprising chlamydospores from at least one dark-colored endophytic fungus, a carrier and auxiliary materials, wherein the content of chlamydospores is more than 2.35 multiplied by 10 8 CFU/g. The invention improves the product property of wettable powder by reasonably optimizing the dosage of DSE chlamydospores and auxiliary materials, the chlamydospores content in the finished product can reach 2.35 multiplied by 10 8 CFU/g, the wetting time is 24.25s, the suspension rate is 73.8%, each index accords with the national standard, the product can be stored for more than 12 months at normal temperature, and the shelf life is prolonged by more than 6 months compared with the conventional conidium bacterial agent. The powder can obviously improve the expression of PAL, PPO, POD, CAT and SOD five defensive enzymes in tomato and rice plants, thereby activating the defensive system of the plants, effectively preventing and treating tomato bacterial wilt and southern rice black-streaked dwarf, improving the growth vigor of tomato infected with bacterial wilt and rice plants infected with black-streaked dwarf, and being suitable for popularization and application as novel biopesticide products.

Inventors

  • ZHANG YAN
  • XIE LING
  • ZENG FENGHUA
  • LONG YANYAN
  • XIE FEI
  • ZHAO HUILI

Assignees

  • 广西壮族自治区农业科学院

Dates

Publication Date
20260512
Application Date
20260410

Claims (10)

  1. 1. The DSE chlamydospore wettable powder is characterized by comprising chlamydospores and carriers from at least one dark-colored isolated endophytic fungus, and further comprising a dispersing agent and a wetting agent accounting for 1-10% of the total mass fraction and an ultraviolet protective agent accounting for 0.5-2.0% of the mass fraction, wherein the content of chlamydospores in the DSE chlamydospore wettable powder is more than 2.35 multiplied by 10 8 CFU/g.
  2. 2. The DSE chlamydospore wettable powder according to claim 1, characterized by said dark-colored isolated endophytic fungi comprising phialides, said phialides comprising phialides guangxi (Cladophialophora guangxiense) HX2 strain with the preservation number CGMCC No.41498.
  3. 3. The DSE chlamydospore wettable powder according to claim 1, wherein the carrier is white carbon black; the mass ratio of the dispersing agent to the wetting agent is 5:1-1:5; the dispersing agent is PEG8000, the wetting agent is Tween 60, and the mass ratio of the dispersing agent to the wetting agent is 1:2-1:4 or 1:3; The total mass fraction of the dispersing agent and the wetting agent in the DSE chlamydospore wettable powder is 5-8%; The ultraviolet protective agent is ascorbic acid, and the mass fraction of the ascorbic acid in the DSE chlamydospore wettable powder is 0.5-1.5% or 1.0%.
  4. 4. A method for preparing the DSE chlamydospore wettable powder according to any one of claims 1 to 3, comprising the steps of: (1) Preparing chlamydospore pulp, namely culturing a dark-colored isolated endophytic fungus strain in a liquid basic culture medium, and collecting chlamydospores in fermentation liquor to obtain chlamydospore pulp; The preparation method of the chlamydospore pulp comprises inoculating a dark-colored endophytic fungus strain on a PDA culture medium plate, performing activation culture at 28 ℃, transferring to a basic culture medium, and culturing in a constant-temperature shaking table at 28 ℃ for 14d; (2) Preparing mother powder, namely adding a carrier subjected to high-temperature sterilization into the chlamydospore pulp to ensure that the mass ratio of chlamydospore prize to the carrier is 1:3, putting the chlamydospore pulp added with the carrier into a baking oven with the temperature of 40+/-1 ℃ for baking for 6 hours, and grinding into powder to obtain the mother powder; (3) The preparation of finished powder, namely adding a dispersing agent and a wetting agent according to a proportion, adding an ultraviolet protective agent according to a proportion, and uniformly mixing to obtain the finished DSE chlamydospore wettable powder.
  5. 5. Use of DSE chlamydospore wettable powder according to any one of claims 1 to 3, for promoting expression of plant defense enzymes, including PAL, PPO, POD, CAT and SOD.
  6. 6. The application of the DSE chlamydospore wettable powder in preventing and treating southern rice black streaked dwarf disease as claimed in any one of claims 1 to 3, wherein the application method of the DSE chlamydospore wettable powder is that the DSE chlamydospore wettable powder is diluted to the concentration of chlamydospores of 1X 10 5 ~1×10 6 CFU/mL.
  7. 7. The application of the DSE chlamydospore wettable powder in preventing and treating tomato bacterial wilt according to any one of claims 1-3, wherein the application method of the DSE chlamydospore wettable powder is that the DSE chlamydospore wettable powder is diluted to the concentration of chlamydospores of 1X 10 5 ~1×10 8 CFU/mL or 1X 10 5 ~1×10 7 CFU/mL.
  8. 8. Use of DSE chlamydospore wettable powder according to any one of claims 1 to 3, for promoting the growth of affected plants, including at least one of rice infected with southern rice black streaked dwarf virus and tomatoes infected with ralstonia solanacearum.
  9. 9. The use according to claim 8, wherein the plant is tomato and the DSE chlamydospore wettable powder is applied by diluting the DSE chlamydospore wettable powder to a chlamydospore concentration of 1 x 10 5 ~1×10 8 CFU/mL or 1 x 10 5 ~1×10 7 CFU/mL.
  10. 10. The use according to claim 9, characterized in that the plant is rice and the DSE chlamydospore wettable powder is applied by diluting the DSE chlamydospore wettable powder to a concentration of 1 x 10 5 ~1×10 6 CFU/mL chlamydospore.

Description

DSE chlamydospore wettable powder and preparation method and application thereof Technical Field The invention belongs to the technical field of microbial preparations, and particularly relates to DSE chlamydospore wettable powder, a preparation method and application thereof. Background Tomato and rice are used as economic crops and grain crops which are important in China and even worldwide, and the yield and quality of the tomato and rice are directly related to the safety of agricultural production, the stable supply of grain and the economic benefits of peasants. However, in the intensive and large-scale planting mode, the frequency and spread of soil-borne diseases and virus diseases have become critical problems for restricting the quality and the high yield of two crops. Wherein, tomato bacterial wilt is caused by Ralstonia solanacearum (Ralstonia solanacearum), is a typical soil-borne vascular bundle disease, has the characteristics of high transmission speed, wide host range, high control difficulty and the like, and can cause rapid wilting and death of tomato plants after the disease, the incidence of common fields can reach 20% -30%, serious plots and even dead plants, and the serious economic loss is caused for the tomato industry. The southern rice black-streaked dwarf disease is a viral disease caused by southern rice black-streaked dwarf virus (Southern rice black-STREAKED DWARF virus, SRBSDV), is mainly transmitted by sogatella furcifera (Sogatella furcifera), and the diseased rice is characterized by dwarf plants, dark green leaves, strong stalks, no heading or unsmooth heading, and has been popular in the main production area of southern rice in China for multiple outbreaks in recent years, thus seriously threatening the rice yield and aggravating the pressure of grain production. At present, the biocontrol microorganism aiming at tomato bacterial wilt mainly uses bacillus, for example, bacillus subtilis SB10 bacterial body weight suspension and fermentation liquor separated from Liu Yumin and the like (2024) have preventive and therapeutic effects on bacterial wilt. Mekonnen et al (2022) found that bacillus isolate BDUA1 could significantly reduce the incidence of bacterial wilting of tomatoes under greenhouse conditions. The problems of weak colonization capability and unstable control effect of the biocontrol bacteria generally exist due to complex field soil environment and high differentiation of physiological race of the bacterial wilt bacteria. The prevention and treatment of southern rice black streaked dwarf mainly takes chemical agents such as imidacloprid, profenofos, pymetrozine and the like as main agents, and the research on biological agents is relatively less. Therefore, in order to find high-efficiency, safe and environment-friendly biological control resources, the development of novel biological pesticides has become a research hotspot and urgent need in the field of green prevention and control of agricultural diseases. The endophyte (DARK SEPTATE Endophytes, DSE) with dark color is a kind of endophyte group which is widely planted in the inside or on the surface of plant root system and forms dark color with hypha and microsclerotium, and can form symbiotic relation with various plants. Research shows that DSE can enhance the tolerance of plants to biotic and abiotic stress through the ways of promoting nutrient absorption, secreting growth regulating substances, inducing plant resistance, inhibiting pathogenic bacteria growth and the like, and has great application potential in biological control of crop diseases. In the prior studies, for the use of fungi, mycelium is often inoculated directly into plants or conidia are used for preparing a microbial inoculum. However, mycelia are easy to deactivate in the practical application process, the shelf life is very short (only 3-6 months), commercial requirements are hardly met, the shelf life of the conventional conidiophore fungicide is also limited, and large-scale application is difficult. Disclosure of Invention In order to solve the technical problems, the invention provides DSE chlamydospore wettable powder, and a preparation method and application thereof. The invention provides DSE chlamydospore wettable powder, which comprises chlamydospores and carriers from at least one dark-colored isolated endophytic fungus, a dispersing agent and a wetting agent accounting for 1-10% of the total mass fraction, and an ultraviolet protective agent accounting for 0.5-2.0% of the mass fraction, wherein the content of chlamydospores in the DSE chlamydospore wettable powder is more than 2.35 multiplied by 10 8 CFU/g. Further, the dark-colored endophytic fungus is Cladosporium armigerum, preferably, the dark-colored endophytic fungus is Cladosporium guangdingii (Cladophialophora guangxiense) HX2 strain with the preservation number of CGMCC No.41498. The Mortierella guangxi (Cladophialophora guangxiense) HX2 is DSE separated and sc