CN-122004248-A - Insecticidal composition containing entomopathogenic virus and methyl jasmonate or jasmonic acid

Abstract

The invention discloses an insecticidal composition containing entomopathogenic viruses and methyl jasmonate or jasmonic acid, and relates to the technical field of biological insecticidal, wherein the active ingredients of the insecticidal composition are entomopathogenic viruses and methyl jasmonate or jasmonic acid, and the entomopathogenic viruses are nuclear polyhedrosis viruses of baculovirus. The weight ratio of the virus to methyl jasmonate or jasmonic acid is 1: (0.5-1000), and the insecticidal composition can be prepared into suspending agents, water dispersible granules, wettable powder and other dosage forms. The invention also provides a method for controlling lepidopteran pests, comprising administering the entomopathogenic virus in combination with methyl jasmonate or jasmonic acid to the target pest or its environment, the combination being simultaneous or sequentially spaced. The invention has synergistic effect on lepidoptera pests such as cotton bollworms, asparagus caterpillars and the like, can obviously reduce the feed intake, accelerate the death of the pests and improve the field control effect, and has wide application prospect.

Inventors

• LIU XIANGYANG
• MIAO PU
• WU DONGLING
• SONG ZHENGXIONG
• ZHANG YUNING
• HE RUI
• Hao Youwu

Assignees

• 河南省烟草公司洛阳市公司

Dates

Publication Date

20260512

Application Date

20251230

Claims (10)

1. 1. An insecticidal composition is characterized by comprising entomopathogenic viruses and methyl jasmonate or jasmonic acid as active ingredients.
2. 2. The insecticidal composition of claim 1, wherein said entomopathogenic virus is a baculovirus.
3. 3. The insecticidal composition of claim 2, wherein said baculovirus is a nuclear polyhedrosis virus.
4. 4. The insecticidal composition according to claim 3, wherein said nuclear polyhedrosis virus is selected from the group consisting of cotton bollworm nuclear polyhedrosis virus, beet armyworm nuclear polyhedrosis virus and cabbage looper nuclear polyhedrosis virus.
5. 5. The insecticidal composition according to claim 1, wherein the mass ratio of the entomopathogenic virus to methyl jasmonate or jasmonic acid is 1: (0.5-1000).
6. 6. The insecticidal composition according to claim 5, wherein the mass ratio is 1:5-500.
7. 7. The insecticidal composition according to claim 1, wherein the composition further comprises an agropharmaceutically acceptable carrier and an auxiliary agent, and the preparation is a suspending agent, an aqueous emulsion, water dispersible granules or wettable powder.
8. 8. A method for preventing and controlling lepidoptera pests is characterized in that methyl jasmonate or jasmonic acid and insect baculovirus are combined to be applied to target pests or living environments thereof, wherein the combined application is that the insect pathogenic virus and the methyl jasmonate or the jasmonic acid are simultaneously applied, or the insect pathogenic virus is firstly applied, and then the methyl jasmonate or the jasmonic acid is applied after 1-7 days apart.
9. 9. The method of claim 8, wherein the lepidopteran pest is cotton bollworm, asparagus caterpillar or spodoptera frugiperda.
10. 10. Use of the pesticidal composition according to any one of claims 1 to 7 for controlling lepidopteran pests.

Description

Insecticidal composition containing entomopathogenic virus and methyl jasmonate or jasmonic acid Technical Field The invention relates to the technical field of biological disinsection, in particular to an insecticidal composition containing entomopathogenic viruses and methyl jasmonate or jasmonic acid. Background Lepidopteran pests, such as cotton bollworm (Helicoverpa armigera), asparagus caterpillar (Spodoptera exigua), spodoptera frugiperda (Spodoptera frugiperda), and the like, are important biodisaster causing factors threatening global agricultural production. The pest larvae directly gnaw plant leaves to damage a photosynthetic system, so that crop biomass accumulation is blocked, and researches show that the fresh weight of a single plant of the damaged plant can be reduced by 37% -62%. In addition, the commodity rate loss of agricultural products caused by the pollution of wormholes and worm manure reaches 15% -40%, and the probability of pathogen infection can be improved by 2.8 times by feeding the wound, so that compound disasters are easy to cause. Therefore, the prevention and control of the plant growth regulator have become key topics for guaranteeing the sustainable development of agriculture. At present, although the prevention and the control of lepidoptera pests show a diversified trend, chemical pesticides are still mainly used, and the prevention and the control of lepidoptera pests account for about 65% of the global market share. The lepidopteran pest resistance increases exponentially under long-term high-intensity selection pressure-for example, the multiple of resistance of plutella xylostella to pyrethroid has exceeded 5000 times. More serious, the sampling inspection of agricultural product residues in 2022 of China shows that the exceeding rate of vegetables reaches 8.7 percent, and the rising rate of the vegetables is 3.2 percent compared with 2015, so that the ecological risk of chemical control is highlighted. Therefore, development of a novel biological agent which is highly targeted and environmentally friendly is an urgent industrial demand. Insect baculoviruses (baculoviruses) are widely recognized as an ideal alternative to chemical pesticides as a class of pathogens that are specifically parasitic to arthropods. Baculoviruses are a class of double-stranded DNA viruses that are specifically parasitic to arthropods, and are named because of their rod-shaped viral particles. The virus has the advantages of strong host specificity, high pathogenicity, safety to non-target organisms, good environmental compatibility, recycling, difficult initiation of drug resistance and the like, and is recommended by the grain and agriculture organizations of the united nations as a biological pesticide with great application potential. The baculovirus can not only realize the continuous compression of insect population by reducing the reproductive capacity and the service life of host insects and vertically spreading in the population, but also realize the continuous prevention and control effect of 'one-time application and multi-generation prevention and control' by spreading the liquid of dead insects to spread the viruses in the environment and forming the circular infection after feeding by the next-generation insect. In addition, baculovirus is proliferated by depending on living insects or cell culture, and the production of the baculovirus does not need chemical synthesis raw materials, so that the problem of three wastes in chemical pesticide production is avoided, and the production requirements of cleanliness and low carbonization are met. Therefore, baculoviruses have become one of the hot spots for the development of biopesticides worldwide. For example, 32 baculovirus pesticide products (EPA, www.epa.gov, 2024) have been registered in the united states, 16 registered in canada (PMRA, www.canada.ca, 2024). In China, baculovirus pesticides are also an important development direction due to the high compliance with the green agricultural demand, the number of registered products is nearly 80 (Chinese pesticide information network, http:// www.icama.org.cn /), and the trend of years is increasing, wherein the registration growth rate in China nearly five years reaches 18.7%, which is remarkably higher than the global average level (9.4%). However, compared with chemical pesticides, the limitations of relatively low insecticidal activity, low insecticidal speed and the like limit the development of baculovirus pesticides, for example, the yield of the virus pesticide preparation in China in 2023 is about 2600 tons, and the yield is only about 2.3 percent of the market share of the pesticide, and the ratio is obviously lower. Jasmonic acid (Jasmonic acid, JA) and its derivative methyl jasmonate (Methyl Jasmonate, meJA) are plant endogenous hormones capable of inducing plants to turn on direct and indirect defenses. When a plant is affected by external adverse environmental fact