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CN-122004251-A - Traditional Chinese medicine active extract for preventing and treating asparagus stem blight and preparation method thereof

CN122004251ACN 122004251 ACN122004251 ACN 122004251ACN-122004251-A

Abstract

The invention discloses a traditional Chinese medicine active extract for preventing and treating asparagus stem blight and a preparation method thereof, belonging to the technical field of crop planting, and comprising the following raw materials, by weight, 20-30 parts of sweet wormwood, 15-20 parts of ‌ radix scutellariae, 15-25 parts of ‌ rhizoma smilacis glabrae, 8-12 parts of ‌ radix sophorae flavescentis, 9-17 parts of stephania tetrandra, 2-5 parts of perilla, 10-15 parts of dyers woad leaf, 8-10 parts of cyrtomium rhizome and 3-5 parts of indigo naturalis. The preparation method comprises the steps of crushing the raw materials, uniformly mixing, adding ethanol solution, performing ultrasonic extraction, performing aerobic fermentation on the obtained extract, and finally evaporating and concentrating the obtained fermentation broth and performing spray drying to obtain the traditional Chinese medicine active extract. The invention effectively extracts active ingredients by selecting various Chinese herbal medicine raw materials, optimizing the proportion and combining the processes of crushing, mixing, ethanol ultrasonic extraction, aerobic fermentation, spray drying and the like. Provides a high-efficiency and natural solution for preventing and treating the stem blight of asparagus.

Inventors

  • YANG YINGQING
  • LIANG YUYONG
  • LAN BO
  • Duan Lingtao
  • YIN CHANGFA
  • WANG XI
  • LIU ZHEHUI
  • ZHANG XU
  • WANG ZHENGYANG
  • SHU CANWEI

Assignees

  • 江西省农业科学院植物保护研究所

Dates

Publication Date
20260512
Application Date
20260203

Claims (10)

  1. 1. A traditional Chinese medicine active extract for preventing and treating asparagus stem blight is characterized by comprising the following raw materials in parts by weight: 20-30 parts of sweet wormwood herb, 15-20 parts of ‌ baical skullcap root, 15-25 parts of ‌ glabrous greenbrier rhizome, 8-12 parts of ‌ kuh-seng, 9-17 parts of tetrandra root, 2-5 parts of purple perilla, 10-15 parts of dyers woad leaf, 8-10 parts of cyrtomium rhizome and 3-5 parts of natural indigo.
  2. 2. The traditional Chinese medicine active extract for preventing and treating asparagus stem blight according to claim 1, which is characterized by comprising the following raw materials in parts by weight: 23-28 parts of sweet wormwood herb, 16-19 parts of ‌ baical skullcap root, 17-23 parts of ‌ glabrous greenbrier rhizome, 9-11 parts of ‌ lightyellow sophora root, 12-15 parts of stephania tetrandra, 3-4 parts of purple perilla, 12-14 parts of dyers woad leaf, 8-9 parts of cyrtomium rhizome and 3-4 parts of natural indigo.
  3. 3. The traditional Chinese medicine active extract for preventing and treating asparagus stem blight according to claim 1, which is characterized by comprising the following raw materials in parts by weight: 25 parts of sweet wormwood, 18 parts of ‌ baical skullcap root, 20 parts of ‌ glabrous greenbrier rhizome, 10 parts of ‌ kuh-seng, 13 parts of stephania tetrandra root, 3 parts of purple perilla, 13 parts of dyers woad leaf, 8 parts of cyrtomium rhizome and 3 parts of natural indigo.
  4. 4. The method for preparing the active extract of the traditional Chinese medicine for preventing and treating the stem blight of asparagus as claimed in claim 1, which is characterized by comprising the following steps: pulverizing the raw materials, mixing uniformly, adding ethanol solution, performing ultrasonic extraction, performing aerobic fermentation on the obtained extract, evaporating and concentrating the obtained fermentation broth, and spray-drying to obtain the traditional Chinese medicine active extract.
  5. 5. The method for preparing a traditional Chinese medicine active extract for preventing and treating asparagus stem blight according to claim 4, wherein the mass concentration of the ethanol solution is 60%, and the addition amount is (8-10) of feed liquid ratio.
  6. 6. The method for preparing the traditional Chinese medicine active extract for preventing and treating asparagus stem blight according to claim 4, wherein the ultrasonic extraction specifically comprises the following steps: filtering after extracting under ultrasonic condition, adding ethanol solution into the residue, repeating the extraction process, and mixing filtrates after extraction to obtain extractive solution.
  7. 7. The method for preparing a Chinese medicinal active extract for preventing and treating asparagus stem blight according to claim 6, wherein the power of the ultrasound is 200-500W; the extraction temperature is 45-55deg.C, and the extraction time is 0.5-1.5h.
  8. 8. The method for preparing a Chinese medicinal active extract for preventing and treating stem blight of asparagus as claimed in claim 4, wherein streptococcus lactis is inoculated in the aerobic fermentation process.
  9. 9. The method for preparing a Chinese medicinal active extract for preventing and treating stem blight of asparagus as claimed in claim 4, wherein the aerobic fermentation is carried out at a temperature of 35-40 ℃ for 45-50h.
  10. 10. The method for preparing a traditional Chinese medicine active extract for preventing and treating asparagus stem blight according to claim 4, wherein the method further comprises the following steps before spray drying: And mixing vitamin E and beta-cyclodextrin into the concentrated solution obtained after evaporation and concentration.

Description

Traditional Chinese medicine active extract for preventing and treating asparagus stem blight and preparation method thereof Technical Field The invention belongs to the technical field of crop planting, and particularly relates to a traditional Chinese medicine active extract for preventing and treating asparagus stem blight and a preparation method thereof. Background The stem blight of asparagus is a serious disease caused by fungal pathogens (such as Phomopsis asparagi) and seriously affects the yield and quality of asparagus. The method for preventing and controlling the stem blight of the asparagus in the prior art mainly comprises various means such as agricultural prevention and control, chemical prevention and control, biological prevention and control and the like. In the aspect of agricultural control, measures such as rotation, removing disease residues, reasonably close planting, improving field drainage and the like are generally adopted to reduce the spread and accumulation of pathogenic bacteria. However, although these methods can reduce the occurrence rate of diseases to some extent, their effects are limited by environmental conditions and the level of planting management, and it is difficult to thoroughly control the spread of diseases. Chemical control is the most widely used method at present, and the growth and reproduction of pathogenic bacteria are inhibited mainly by spraying bactericides (such as carbendazim, mancozeb and the like). Although the chemical control has quick and obvious effect, the long-term use can lead pathogenic bacteria to generate drug resistance, and the control effect is reduced. Meanwhile, excessive use of chemical agents may also adversely affect the environment and human health, such as soil pollution, water source pollution, and damage to non-target organisms, contrary to the concept of sustainable agricultural development. Biological control has received increasing attention in recent years as an environmentally friendly alternative. The method utilizes antagonistic microorganisms (such as Trichoderma, bacillus, etc.) or plant extracts to inhibit the growth of pathogenic bacteria. Biological control has the advantages of high safety, difficult generation of drug resistance and the like, but the control effect is greatly influenced by environmental factors, the action mechanism is complex, and the problems of unstable effect, high cost and the like in practical application are often caused. In addition, breeding disease-resistant varieties is also an important control strategy. The asparagus variety with strong disease resistance is cultivated by the traditional breeding or molecular breeding technology, so that the occurrence of diseases can be fundamentally reduced. However, the breeding period of disease-resistant varieties is long, the cost is high, and the disease resistance is lost due to the variation of pathogenic bacteria, so that the wide application of the disease-resistant varieties is limited. Therefore, how to provide a method for effectively preventing and controlling the stem blight of asparagus, which can not only effectively control diseases, but also meet the requirements of environmental protection and sustainable development, is a technical problem to be solved by the technicians in the field. Disclosure of Invention In order to solve the technical problems, the invention provides a traditional Chinese medicine active extract for preventing and treating asparagus stem blight and a preparation method thereof. In order to achieve the above purpose, the present invention provides the following technical solutions: a traditional Chinese medicine active extract for preventing and treating asparagus stem blight comprises the following raw materials in parts by weight: 20-30 parts of sweet wormwood herb, 15-20 parts of ‌ baical skullcap root, 15-25 parts of ‌ glabrous greenbrier rhizome, 8-12 parts of ‌ kuh-seng, 9-17 parts of tetrandra root, 2-5 parts of purple perilla, 10-15 parts of dyers woad leaf, 8-10 parts of cyrtomium rhizome and 3-5 parts of natural indigo. Preferably, the material comprises the following raw materials in parts by weight: 23-28 parts of sweet wormwood herb, 16-19 parts of ‌ baical skullcap root, 17-23 parts of ‌ glabrous greenbrier rhizome, 9-11 parts of ‌ lightyellow sophora root, 12-15 parts of stephania tetrandra, 3-4 parts of purple perilla, 12-14 parts of dyers woad leaf, 8-9 parts of cyrtomium rhizome and 3-4 parts of natural indigo. Preferably, the material comprises the following raw materials in parts by weight: 25 parts of sweet wormwood, 18 parts of ‌ baical skullcap root, 20 parts of ‌ glabrous greenbrier rhizome, 10 parts of ‌ kuh-seng, 13 parts of stephania tetrandra root, 3 parts of purple perilla, 13 parts of dyers woad leaf, 8 parts of cyrtomium rhizome and 3 parts of natural indigo. The traditional Chinese medicine active extract for preventing and treating the stem blight of asparagus has the benef