CN-122004259-A - Pesticide combination for preventing and treating root-knot nematode and application method thereof

Abstract

The application belongs to the technical field of biological pesticides, and provides a pesticide composition for preventing and treating root knot nematodes and an application method thereof. The application discovers that the water extract of common waste eichhornia crassipes can effectively improve the anti-nematode effect of abamectin, especially low-concentration abamectin, and can greatly reduce the dosage/concentration of abamectin when being matched with abamectin for use, thereby realizing remarkable environmental protection benefit.

Inventors

• HAN YING
• CHEN NUO
• FENG JIANLU

Assignees

• 六夫丁作物保护有限公司

Dates

Publication Date

20260512

Application Date

20260205

Claims (10)

1. 1. A pesticide combination for preventing and controlling root knot nematodes, which is characterized by comprising an avermectin preparation and an eichhornia crassipes water extract.
2. 2. The pesticidal combination according to claim 1, said avermectin formulation being a 2-10% avermectin liquid formulation.
3. 3. The pesticidal combination according to claim 2, said avermectin formulation being a 2% avermectin liquid formulation.
4. 4. A pesticidal combination according to any one of claims 1-3, said water extract of eichhornia crassipes being prepared by the process of: (1) Draining water from Eichhornia crassipes, and pulverizing to obtain Eichhornia crassipes fragments; (2) Adding eichhornia crassipes chips into water with the weight 2-4 times of the wet weight of the eichhornia crassipes chips, and stirring and extracting at 85-95 ℃ for 1-3 hours to obtain an extraction mixture; (3) Filtering the extract mixture, and centrifuging at 800-1200g for 5-15 min to obtain water extract of Eichhornia crassipes.
5. 5. The pesticide combination according to claim 4, wherein the water extract of eichhornia crassipes is prepared by the following steps: (1) Draining water from Eichhornia crassipes, and pulverizing to obtain Eichhornia crassipes fragments; (2) Adding eichhornia crassipes chips into water with the weight 3 times of the wet weight of the eichhornia crassipes chips, and stirring and extracting for 2 hours at 90 ℃ to obtain an extraction mixture; (3) Filtering the extract mixture, and centrifuging 1000g for 10 minutes to obtain water extract of Eichhornia crassipes.
6. 6. A method for controlling the meloidogyne incognita of crops, which is characterized in that the method comprises the steps of root irrigation and application of a 4000-6000 times diluted 2% avermectin liquid preparation and a 10 times diluted water extract of eichhornia crassipes.
7. 7. The method according to claim 6, wherein the water extract of eichhornia crassipes is prepared by the following steps: (1) Draining water from Eichhornia crassipes, and pulverizing to obtain Eichhornia crassipes fragments; (2) Adding eichhornia crassipes chips into water with the weight 2-4 times of the wet weight of the eichhornia crassipes chips, and stirring and extracting at 85-95 ℃ for 1-3 hours to obtain an extraction mixture; (3) Filtering the extract mixture, and centrifuging at 800-1200g for 5-15 min to obtain water extract of Eichhornia crassipes.
8. 8. The method according to claim 6, wherein the water extract of eichhornia crassipes is prepared by the following steps: (1) Draining water from Eichhornia crassipes, and pulverizing to obtain Eichhornia crassipes fragments; (2) Adding eichhornia crassipes chips into water with the weight 3 times of the wet weight of the eichhornia crassipes chips, and stirring and extracting for 2 hours at 90 ℃ to obtain an extraction mixture; (3) Filtering the extract mixture, and centrifuging 1000g for 10 minutes to obtain water extract of Eichhornia crassipes.
9. 9. The method of any one of claims 6-8, comprising administering per root irrigation 150-350mL of a 5000-fold diluted 2% avermectin liquid formulation and 100-200mL of a 10-fold diluted water extract of eichhornia crassipes.
10. 10. The method according to any one of claims 6-9, wherein the crop is pepper.

Description

Pesticide combination for preventing and treating root-knot nematode and application method thereof Technical Field The application belongs to the technical field of biological pesticides, and particularly provides a pesticide composition for preventing and treating root-knot nematodes and an application method thereof. Background Root knot nematodes (Meloidogyne) are highly specialized omnivorous plant pathogenic nematodes that can harm a variety of crops including tobacco, cucumber, peanut, radish, cabbage, and the like. The root system of the damaged plant is weakened, white root nodules appear, and the crop growth and the agricultural product production are seriously affected. Root knot nematodes grow in shallow soil and are difficult to find by the naked eye. Often overwintering occurs in the form of eggs and invades young root tissue of plants in the form of second-instar larvae in spring. The deep ploughing treatment of soil can alleviate the root-knot nematode disease, but the effect is not obvious, and pesticides such as abamectin, fosthiazate and the like are often used for stirring or root irrigation in the production. With the technological development and the change of regulations, the toxicity and the damage to soil of the pesticides are low, but the long-term application still has adverse effects on soil, surrounding water environment, beneficial insects and the like, and the continuous need for reducing the pesticide dosage still exists in the field. Eichhornia crassipes (Pontederia crassipes/Eichhornia crassipes) is also called water hyacinth, is native to south-central america, and has medicinal, environmental protection, feeding, fertilizer use and other values. After the water hyacinth is introduced into China in the beginning of the 20 th century, the water hyacinth bursts in a plurality of water areas of China due to low requirement on the growth environment and lack of competition, so that the problems of serious blockage of water transportation, damage to local animals and plants, water eutrophication and the like are caused. The main mode of the current water hyacinth burst is salvage, and the main utilization mode of the current salvaged water hyacinth only has one preparation of organic fertilizer because of the high-cost special treatment of the utilization modes of feeding, papermaking and the like. The ecological competitiveness of eichhornia crassipes is largely derived from components which contain anti-microalgae and insect, and the utilization of the extract thereof for developing pesticides is an effective way for fully utilizing the same. Disclosure of Invention In one aspect, the application provides a pesticide combination for preventing and controlling root knot nematodes, which comprises an avermectin preparation and an eichhornia crassipes water extract. Further, the avermectin preparation is 2-10% of avermectin liquid preparation. Further, the avermectin preparation is 2% avermectin liquid preparation. Further, the preparation method of the water extract of the eichhornia crassipes comprises the following steps: (1) Draining water from Eichhornia crassipes, and pulverizing to obtain Eichhornia crassipes fragments; (2) Adding eichhornia crassipes chips into water with the weight 2-4 times of the wet weight of the eichhornia crassipes chips, and stirring and extracting at 85-95 ℃ for 1-3 hours to obtain an extraction mixture; (3) Filtering the extract mixture, and centrifuging at 800-1200g for 5-15 min to obtain water extract of Eichhornia crassipes. Further, the preparation method of the water extract of the eichhornia crassipes comprises the following steps: (1) Draining water from Eichhornia crassipes, and pulverizing to obtain Eichhornia crassipes fragments; (2) Adding eichhornia crassipes chips into water with the weight 3 times of the wet weight of the eichhornia crassipes chips, and stirring and extracting for 2 hours at 90 ℃ to obtain an extraction mixture; (3) Filtering the extract mixture, and centrifuging 1000g for 10 minutes to obtain water extract of Eichhornia crassipes. Further, the nematode is a meloidogyne incognita. In another aspect, the application provides a method of controlling meloidogyne incognita in crops, the method comprising root irrigation with a 5000-fold dilution of a 2% avermectin liquid formulation and a 10-fold dilution of an aqueous extract of Eichhornia crassipes. Further, the preparation method of the water extract of the eichhornia crassipes comprises the following steps: (1) Draining water from Eichhornia crassipes, and pulverizing to obtain Eichhornia crassipes fragments; (2) Adding eichhornia crassipes chips into water with the weight 2-4 times of the wet weight of the eichhornia crassipes chips, and stirring and extracting at 85-95 ℃ for 1-3 hours to obtain an extraction mixture; (3) Filtering the extract mixture, and centrifuging at 800-1200g for 5-15 min to obtain water extract of Eichhornia crassipes. Further, the preparat