CN-122004286-A - Application of atractylenolide III in preparation of sensing quencher for Serratia liquefaciens population

Abstract

The invention discloses application of atractylenolide III in preparation of a serratia liquefaciens quorum sensing quencher. Belongs to the technical field of medicines. The invention aims to prepare the application of the drug for inhibiting the bacterial quorum sensing system of Serratia liquefaciens, which is used for providing the drug for inhibiting the bacterial quorum sensing system, so that the toxicity of pathogenic bacteria is weakened on the premise of not inhibiting the growth of the pathogenic bacteria. The invention provides application of atractylenolide III in a quencher for inhibiting a serratia liquefaciens quorum sensing system. Provides a brand new solution for developing high-efficiency and safe food biological preservative.

Inventors

• CHEN QIAN
• Zhang Zidai
• ZHANG BIYING
• ZHANG MINGZHU
• LU JIACHENG
• LIU JIAN
• Cao Chuanai
• KONG BAOHUA

Assignees

• 东北农业大学

Dates

Publication Date

20260512

Application Date

20260402

Claims (10)

1. 1. Application of atractylenolide III in preparing quencher for inhibiting quorum sensing system of Serratia liquefaciens.
2. 2. Application of atractylenolide III in preparing quencher for inhibiting formation of extracellular polysaccharide of Serratia liquefaciens.
3. 3. Application of atractylenolide III in preparing quencher for inhibiting extracellular protease activity of Serratia liquefaciens.
4. 4. Application of atractylenolide III in preparing quencher for inhibiting activity of extracellular lipase of Serratia liquefaciens.
5. 5. Application of atractylenolide III in preparing quencher for inhibiting the output of liquefied Serratia cell biomembrane.
6. 6. Application of atractylenolide III in preparing medicines for inhibiting colony, motility or biomembrane morphology of Serratia liquefaciens cells.
7. 7. The use according to any one of claims 1 to 6, wherein the quenching agent is in the form of any one of a tablet, a capsule, a granule, a powder, a liquid.
8. 8. The use according to any one of claims 1 to 6, wherein the concentration of atractylenolide III is 64 μg/mL.
9. 9. Application of atractylenolide III in preparing food preservative is provided.
10. 10. The use according to claim 9, wherein the target is serratia liquefaciens quorum sensing LuxR protein.

Description

Application of atractylenolide III in preparation of sensing quencher for Serratia liquefaciens population Technical Field The invention belongs to the technical field of medicines, and particularly relates to application of atractylenolide III in preparation of a serratia liquefaciens quorum sensing quencher. Background Serratia liquefaciens is a common food-borne spoilage bacterium, and often causes spoilage of meat, aquatic products and the like, so that bacteriostasis research in the food field is most concentrated, and as antibiotics are widely used clinically, the drug resistance generated by bacteria is gradually enhanced, so that the sensitivity of the antibiotics to pathogenic bacteria is continuously reduced, and the physiological and biochemical characteristics of the bacteria can be changed, so that the bacteria generate originally unavailable characteristics, such as drug permeability disorder, drug inactivating enzyme generation, biofilm formation and the like, thereby reducing the action of the antibiotics. At present, antibiotic resistance has become a global serious public health problem. In recent years, bacterial Quorum Sensing (QS) systems have become an important target for the study of novel drug-resistant bacteria drugs. QS is a means of intracellular or intercellular signaling in bacteria to control and coordinate overall bacterial population behavior by monitoring the concentration of certain signaling molecules (also known as auto-inducing molecules) such as homoserine lactones (acyl-homoserine lactone, AHL), which together respond to environmental stimuli, greatly enhancing the viability of the overall bacterial population. Pathogenicity of pathogenic bacteria is regulated by QS, the QS system effects its pathogenicity by mediating expression of disease genes. When the pathogen density reaches a certain degree, the pathogen synthesizes and releases a certain signal molecule, can start the expression of related genes, and regulate various biological behaviors of the pathogen, such as bioluminescence, toxin generation, biofilm formation, antibiotic generation and the like. Thus, the search for drugs that inhibit the quorum sensing system is a problem to be solved by researchers in the field. Disclosure of Invention The invention aims to prepare the application of the drug for inhibiting the bacterial quorum sensing system of Serratia liquefaciens, which is used for providing the drug for inhibiting the bacterial quorum sensing system, so that the toxicity of pathogenic bacteria is weakened on the premise of not inhibiting the growth of the pathogenic bacteria. The invention provides application of atractylenolide III in preparing a quencher for inhibiting a serratia liquefaciens quorum sensing system. The invention provides an application of atractylenolide III in preparing a quencher for inhibiting formation of extracellular polysaccharide of Serratia liquefaciens. The invention provides application of atractylenolide III in preparing a quencher for inhibiting activity of extracellular protease of Serratia liquefaciens. The invention provides application of atractylenolide III in preparing a quencher for inhibiting activity of extracellular lipase of Serratia liquefaciens. The invention provides an application of atractylenolide III in preparing a quencher for inhibiting the yield of a liquefied Serratia cell biomembrane. The invention provides an application of atractylenolide III in preparing a medicament for inhibiting the aggregation, motility or biomembrane morphology of Serratia liquefaciens cells. Further defined, the formulation of the quenching agent is any one of a tablet, a capsule, a granule, a powder and a liquid preparation. Further defined, the concentration of atractylenolide III is 64 μg/mL. The invention provides application of atractylenolide III in preparation of food preservative. Further defined, the target is serratia liquefaciens quorum sensing LuxR protein. The invention has the beneficial effects that the brand new application of the known traditional Chinese medicine active ingredient of the atractylenolide III in targeting the LuxR protein and inhibiting bacterial quorum sensing is verified, and the effects of inhibiting the formation of a serratia liquefaciens biological film, virulence factors (protease, lipase and the like) and the like in food can be effectively demonstrated under the condition of sub-antibacterial concentration. These independent inhibitors based on edible and medicinal substances provide various innovative and practical candidate solutions for developing food-grade anti-virulence preparations which are safe, accurate and not easy to induce drug resistance. Drawings FIG. 1 shows the effect of different concentrations of atractylenolide III on Serratia liquefaciens growth curve; FIG. 2 shows the effect of different concentrations of atractylenolide III on Serratia liquefaciens exopolysaccharide (A), exolipase (B), biofilm formation (