CN-122004289-A - Allicin-canna edulis ker water extract-bacitracin D composite film for storage and preservation of sugar oranges

CN122004289ACN 122004289 ACN122004289 ACN 122004289ACN-122004289-A

Abstract

The invention relates to the technical field of food packaging materials, and discloses an allicin-canna edulis ker water extract-bacitracin D composite film for storage and preservation of sugar oranges, which is prepared by hydrolyzing fresh garlic, extracting by an ultrasonic auxiliary eutectic solvent method, concentrating to obtain allicin, mixing allicin and bacitracin D with canna edulis ker water extract according to proper proportion, mixing uniformly, ultrasonic defoaming, and drying at constant temperature to obtain the composite membrane. The allicin, the canna edulis ker aqueous extract and the bacitracin D are integrated at the same time, so that the antioxidation activity can be effectively improved, the bacteriostasis rate is further improved, and the prepared allicin-canna edulis ker aqueous extract-bacitracin D composite film has excellent performance, and the preservative effect of the sugar orange can be effectively improved.

Inventors

QIAN SHIQUAN
CHEN WEIWEI
HU YANTING
DIAO ENJIE
ZHANG LIMING
ZHANG YUANG
FAN JINGJING
DAI YINING
ZHENG YOUYOU
SONG WENXING
ZHANG XIN
TANG RUOJUN
XIA QI
CHEN XI

Assignees

淮阴师范学院
江苏黎明食品集团有限公司

Dates

Publication Date: 20260512
Application Date: 20260123

Claims (9)

1. The preparation method of the allicin-canna edulis ker water extract-bacitracin D composite film for the storage and preservation of sugar oranges is characterized by comprising the following steps of: step 1, taking clean fresh garlic, peeling and hydrolyzing, extracting by adopting an ultrasonic-assisted eutectic solvent method, concentrating to obtain allicin, mixing the allicin and triglyceride, adding an emulsifying agent, homogenizing, adding an aqueous solution of trehalose, and mixing to obtain allicin nanoemulsion; step 2, taking Pi Bajiao of taro, mixing with distilled water, pulping, filtering, taking filtrate, centrifuging to obtain a water extract of the canna, mixing the water extract of the canna with bacitracin D, dropwise adding a sodium tripolyphosphate solution, centrifuging, washing, and freeze-drying to obtain bacitracin D-canna water extract nano particles; And 3, adding glycerol into the canna edulis ker water extract, oscillating at constant temperature and carrying out ultrasonic treatment to obtain a canna edulis ker water extract basement membrane solution, adding the allicin nanoemulsion and the bacitracin D-canna edulis ker water extract nanoparticles into the canna edulis ker water extract basement membrane solution, uniformly mixing, removing bubbles by ultrasonic waves, and drying at constant temperature to obtain the allicin-canna edulis ker water extract-bacitracin D composite membrane.
2. The preparation method of the allicin-canna edulis ker water extract-bacitracin D composite film according to claim 1, wherein in the step 1, in the ultrasonic-assisted eutectic solvent method, the extracting agent is choline chloride and xylitol with a molar mass ratio of 1:1, and the extraction condition is that the constant temperature extraction is carried out at 35-45 ℃ under the ultrasonic condition of 200-250W for 30-50 min.
3. The preparation method of the allicin-canna edulis ker water extract-bacitracin D composite film according to claim 1, wherein in the step 1, the mass ratio of the allicin to the triglyceride is 1:3-5; In the step 1, the emulsifier is tween-80 and lecithin with the mass ratio of 2:1.
4. The method for preparing the allicin-canna aqueous extract-bacitracin D composite film according to claim 1, wherein in the step 2, the volume ratio of the canna aqueous extract, bacitracin D and the sodium tripolyphosphate solution is 0.2-1.5:0.2-1.5:0.2-1.5.
5. The preparation method of the allicin-canna aqueous extract-bacitracin D composite film according to claim 1, wherein in the step 3, the volume ratio of the canna aqueous extract base film solution, the allicin nanoemulsion and the bacitracin D-canna aqueous extract nanoparticles is 0.2-1.5:0.2-1.5:0.2-3.0.
6. The preparation method of the allicin-canna edulis ker water extract-bacitracin D composite film according to claim 1, wherein in the step 3, the constant-temperature oscillation condition is that the temperature is 30-40 ℃, the rotating speed is 110-130 rpm and the time is 10-14 h.
7. The method for preparing the allicin-canna aqueous extract-bacitracin D composite film according to claim 1, wherein the glycerol is added in an amount of 2.0-3.0ml in the step 3.
8. The allicin-canna edulis ker aqueous extract-bacitracin D composite membrane prepared by the method according to any one of claims 1 to 7, wherein the allicin-canna edulis ker aqueous extract-bacitracin D composite membrane is prepared by compounding allicin and bacitracin D and then mixing with the canna edulis ker aqueous extract.
9. Use of an allicin-canna edulis ker water extract-bacitracin D composite film prepared by the method according to any one of claims 1-7 in storage and preservation of sugar oranges.

Description

Allicin-canna edulis ker water extract-bacitracin D composite film for storage and preservation of sugar oranges Technical Field The invention relates to the technical field of food packaging materials, in particular to an allicin-canna edulis ker water extract-bacitracin D composite film for storage and preservation of sugar oranges. Background With the improvement of the requirements of people on food safety and fruit quality, food preservation technology is increasingly paid attention to. Traditional plastic preservative films such as Polyethylene (PE) films have certain preservative effect, but lack of antibacterial activity, so that the growth of microorganisms in the storage process of fruits and vegetables is difficult to effectively inhibit, and the fruits and vegetables are rotten, lost nutrition and reduced in quality are caused. Therefore, development of a safe green composite film with an antibacterial function is particularly necessary. The bacitracin D is a cyclic lipopeptide compound generated by bacillus subtilis and has good broad-spectrum antifungal activity. Allicin is a natural antibacterial component extracted from Bulbus Allii, and has inhibiting effect on various pathogenic bacteria. According to the invention, the bacitracin D and the garlicin are compounded, the canna edulis ker aqueous extract is used as a wall material, the safe and green nanoemulsion (granule) and the composite antibacterial film are prepared, and the antibacterial film is applied to the anticorrosion and fresh-keeping of sugar oranges, and has important significance in improving the fresh-keeping effect of fruits and vegetables and promoting the development of green packaging materials. Disclosure of Invention Aiming at the problems in the prior art, the invention provides an allicin-canna edulis ker water extract-bacitracin D composite film for storage and preservation of sugar oranges. The allicin, the canna edulis ker aqueous extract and the bacitracin D are integrated at the same time, so that the antioxidation activity can be effectively improved, the bacteriostasis rate is further improved, and the prepared allicin-canna edulis ker aqueous extract-bacitracin D composite film has excellent performance, and the preservative effect of the sugar orange can be effectively improved. According to the technical scheme, in the first aspect, the invention provides a preparation method of an allicin-canna edulis ker water extract-bacitracin D composite film for storage and preservation of sugar oranges, which comprises the following steps of: step 1, taking clean fresh garlic, peeling and hydrolyzing, extracting by adopting an ultrasonic-assisted eutectic solvent method, concentrating to obtain allicin, mixing the allicin and triglyceride, adding an emulsifying agent, homogenizing, adding an aqueous solution of trehalose, and mixing to obtain allicin nanoemulsion; step 2, taking Pi Bajiao of taro, mixing with distilled water, pulping, filtering, taking filtrate, centrifuging to obtain a water extract of the canna, mixing the water extract of the canna with bacitracin D, dropwise adding a sodium tripolyphosphate solution, centrifuging, washing, and freeze-drying to obtain bacitracin D-canna water extract nano particles; And 3, adding glycerol into the canna edulis ker water extract, oscillating at constant temperature and carrying out ultrasonic treatment to obtain a canna edulis ker water extract basement membrane solution, adding the allicin nanoemulsion and the bacitracin D-canna edulis ker water extract nanoparticles into the canna edulis ker water extract basement membrane solution, uniformly mixing, removing bubbles by ultrasonic waves, and drying at constant temperature to obtain the allicin-canna edulis ker water extract-bacitracin D composite membrane. Further, in the step 1, in the ultrasonic-assisted eutectic solvent method, the extracting agent is choline chloride and xylitol with a molar mass ratio of 1:1, and the extracting condition is that the constant-temperature extraction is carried out at 35-45 ℃ under the ultrasonic condition of 200-250W for 30-50 min. Further, in the step 1, the mass ratio of the allicin to the triglyceride is 1:3-5; In the step 1, the emulsifier is tween-80 and lecithin with the mass ratio of 2:1. Further, in the step 2, the volume ratio of the canna edulis ker water extract, the bacitracin D and the sodium tripolyphosphate solution is 0.2-1.5:0.2-1.5:0.2-1.5. Further, in the step 3, the volume ratio of the canna edulis ker aqueous extract basement membrane solution, the allicin nanoemulsion and the bacitracin D-canna edulis ker aqueous extract nanoparticles is 0.2-1.5:0.2-1.5:0.2-3.0. Further, in the step 3, the constant temperature oscillation condition is that the temperature is 30-40 ℃, the rotating speed is 110-130 rpm, and the time is 10-14 h. Further, in the step 3, the glycerol is added in an amount of 2.0-3.0ml. In a second aspect, the invention provides an allicin-cann