CN-122004301-A - Composite bioactive peptide composition for improving joint injury and preparation method thereof

CN122004301ACN 122004301 ACN122004301 ACN 122004301ACN-122004301-A

Abstract

The invention relates to the technical field of health-care food, in particular to a composite bioactive peptide composition for improving joint injury and a preparation method thereof, wherein the raw materials of the composition comprise: the yak bone oligopeptide, the yak bone marrow, the whole milk, the bovine brain peptide, the hyaluronic acid, the tea polyphenol and the sweetener.

Inventors

WANG ZHISEN
CHEN JUN
ZHANG HUICONG
ZHAO BINGLING
LI SAI
ZHANG XINYANG

Assignees

石家庄藏诺药业股份有限公司

Dates

Publication Date: 20260512
Application Date: 20260403

Claims (10)

1. A composite bioactive peptide composition for improving joint injury is characterized by comprising the raw materials of yak bone oligopeptide, yak bone marrow, whole milk, bovine brain peptide, hyaluronic acid, tea polyphenol and sweetener.
2. The composite bioactive peptide composition of claim 1, wherein the starting material of the composition further comprises phosphoserine, a nervonic acid, a modified chitosan, food grade silica, and modified corn starch.
3. The composite bioactive peptide composition of claim 1, wherein the composition comprises 3-10 parts of yak bone oligopeptide, 10-20 parts of yak bone marrow, 50-70 parts of whole milk, 0.5-1.5 parts of bovine brain peptide, 0.5-1 part of hyaluronic acid, 0.1-0.5 part of tea polyphenol and 10-20 parts of sweetener.
4. The composite bioactive peptide composition of claim 2, wherein the composition comprises 0.3-1 part of phosphatidylserine, 0.2-0.5 part of nervonic acid, 3-8 parts of modified chitosan, 0.05-0.1 part of food grade silicon dioxide and 20-40 parts of modified corn starch.
5. The composite bioactive peptide composition of claim 2, wherein said modified chitosan is grafted with succinyl and hydroxypropyl groups.
6. The composite bioactive peptide composition of claim 2, wherein the modified corn starch incorporates phosphate cross-linking groups and octenyl succinic groups.
7. The composite bioactive peptide composition of claim 2, wherein the food grade silica is bioactive silicon, the bioactive silicon being silicate and/or organosilicon.
8. The composite bioactive peptide composition of any of claims 1-7, wherein said composition is useful for ameliorating bone joint damage and is applicable to the acute phase, recovery phase and maintenance phase of bone joint damage.
9. The composite bioactive peptide composition of any of claims 1-7, wherein the composition is in a dosage form for oral administration.
10. A method of preparing a composite bioactive peptide composition for improving joint injury, wherein the method of preparing the composition is used to prepare the composition of any one of claims 1-9, the method of preparing the composition comprising: The preparation method comprises the steps of S1, preprocessing, namely dissolving active peptide, emulsifying fat-soluble components, emulsifying modified chitosan and dispersing silicon dioxide, wherein the active peptide dissolving is to add 50% of pure water in formula amount after the yak bone oligopeptide and the bovine brain peptide are mixed, and stir the solution to obtain peptide aqueous phase mother liquor, the fat-soluble components are to mix the phosphatidylserine, the nervonic acid and the yak bone marrow, add the modified corn starch, dissolve the modified corn starch with polyethylene glycol, and then perform high-speed shearing emulsification to obtain fat-soluble mother liquor, the modified chitosan is to add 10% of pure water in formula amount, stir the modified chitosan in 50 ℃ water bath until the modified chitosan is completely dissolved, and the silicon dioxide dispersing is to add 5% of pure water in formula amount to obtain silicon dioxide dispersing liquid in an ultrasonic way; S2, adding the chitosan aqueous solution and the fat-soluble mother solution into the peptide aqueous phase mother solution, uniformly stirring, adding the silicon dioxide dispersion liquid and the hyaluronic acid, and uniformly stirring to obtain a mixed emulsion; s3, a high-pressure homogenizing step, namely transferring the mixed emulsion into a high-pressure homogenizer for homogenizing twice; S4, an antioxidation step, namely adding the tea polyphenol and the sweetener, stirring and dissolving, adjusting the pH value to 6.0-6.5, and adding pure water to a preset volume; S5, filtering and clarifying, namely filtering and removing impurities and large particles through a microporous filter membrane; s6, sterilizing at low temperature, sterilizing by a pasteurization method, and preserving heat for 30min at 60 ℃; and S7, in the aseptic filling stage, after low-temperature sterilization is finished, cooling to room temperature, and carrying out aseptic filling to obtain the compound bioactive peptide composition solution.

Description

Composite bioactive peptide composition for improving joint injury and preparation method thereof Technical Field The invention belongs to the technical field of health-care foods, and particularly relates to a composite bioactive peptide composition for improving joint injury and a preparation method thereof. Background With the development of the health industry, bioactive peptides derived from natural products have been attracting attention due to their excellent functional properties. The yak marrow peptide has obvious potential in relieving joint abrasion, repairing cartilage tissues and improving osteoarthritis symptoms due to unique amino acid sequences and rich nutrition, and is a joint health functional raw material with great development prospect. Currently, products for bone joint diseases in the market generally take a single main component for conditioning bone joints, for example, ammonia sugar is utilized to realize bone joint repair, and the conditioning effect is not ideal due to the method of conditioning by the single component. Disclosure of Invention (1) Technical problem to be solved The invention aims to provide a compound bioactive peptide composition for improving joint injury and a preparation method thereof, which aim to solve the problems that products aiming at bone joint diseases in the market generally take a single main component for conditioning bone joints, for example, ammonia sugar is utilized for realizing bone joint repair, and the conditioning effect is not ideal due to the method of conditioning by the single component. (2) Technical proposal In order to achieve the aim, in one aspect, the invention provides a composite bioactive peptide composition for improving joint injury, which comprises the following raw materials of yak bone oligopeptide, yak bone marrow, whole milk, bovine brain peptide, hyaluronic acid, tea polyphenol and sweetener. Further, the composition further comprises phosphatidylserine, nervonic acid, modified chitosan, food grade silica and modified corn starch. Further, the composition comprises 3-10 parts of yak bone oligopeptide, 10-20 parts of yak bone marrow, 50-70 parts of whole milk, 0.5-1.5 parts of bovine brain peptide, 0.5-1 part of hyaluronic acid, 0.1-0.5 part of tea polyphenol and 10-20 parts of sweetener. Further, the composition comprises 0.3-1 part of phosphatidylserine, 0.2-0.5 part of nervonic acid, 3-8 parts of modified chitosan, 0.05-0.1 part of food-grade silicon dioxide and 20-40 parts of modified corn starch. Further, the modified chitosan is grafted with succinyl and hydroxypropyl. Further, the modified corn starch incorporates phosphate cross-linking groups and octenyl succinic groups. Further, the food grade silica is bioactive silicon, which is silicate and/or organosilicon. Further, the composition is used for improving bone joint injury, and can be applied to the acute phase, the recovery phase and the maintenance phase of bone joint injury. Further, the formulation of the composition is oral. The invention also provides a preparation method of the composite bioactive peptide composition for improving joint injury, which is used for preparing the composition according to the claim, and comprises the following steps: The preparation method comprises the steps of S1, preprocessing, namely dissolving active peptide, emulsifying fat-soluble components, emulsifying modified chitosan and dispersing silicon dioxide, wherein the active peptide dissolving is to add 50% of pure water in formula amount after the yak bone oligopeptide and the bovine brain peptide are mixed, and stir the solution to obtain peptide aqueous phase mother liquor, the fat-soluble components are to mix the phosphatidylserine, the nervonic acid and the yak bone marrow, add the modified corn starch, dissolve the modified corn starch with polyethylene glycol, and then perform high-speed shearing emulsification to obtain fat-soluble mother liquor, the modified chitosan is to add 10% of pure water in formula amount, stir the modified chitosan in 50 ℃ water bath until the modified chitosan is completely dissolved, and the silicon dioxide dispersing is to add 5% of pure water in formula amount to obtain silicon dioxide dispersing liquid in an ultrasonic way; S2, adding the chitosan aqueous solution and the fat-soluble mother solution into the peptide aqueous phase mother solution, uniformly stirring, adding the silicon dioxide dispersion liquid and the hyaluronic acid, and uniformly stirring to obtain a mixed emulsion; s3, a high-pressure homogenizing step, namely transferring the mixed emulsion into a high-pressure homogenizer for homogenizing twice; S4, an antioxidation step, namely adding the tea polyphenol and the sweetener, stirring and dissolving, adjusting the pH value to 6.0-6.5, and adding pure water to a preset volume; S5, filtering and clarifying, namely filtering and removing impurities and large particles through a microporous filter membrane; s6, ste