CN-122004348-A - Application of Pediococcus acidilactici NR-combined complex enzyme preparation in preparation of wheat bran starter and fermentation method thereof

Abstract

The invention discloses an application of a Pediococcus acidilactici NR-combined complex enzyme preparation in preparing a wheat bran starter and a fermentation method thereof, belonging to the field of fermentation engineering and feed processing. The invention provides a method for improving the protein content and the protein digestibility of fermented wheat bran by synergistic fermentation of Pediococcus acidilactici and a compound enzyme preparation, which obviously improves the acid-soluble protein content and the in-vitro protein digestibility of wheat bran by fermentation technology. In the actual animal feeding test, the fermented wheat bran shows excellent feed substitution effect, can partially replace corn and soybean meal, remarkably improves the feed digestibility and reduces the feed cost. The invention provides a new way for the high-value utilization of wheat bran, and has wide application prospect and important economic significance.

Inventors

• YANG HUIQING
• LIANG YUNXIANG
• ZHANG HENG
• Xie Aowen
• MEI YUXIA
• CHEN ZHENMIN

Assignees

• 华中农业大学

Dates

Publication Date

20260512

Application Date

20260326

Claims (10)

1. 1. Use of pediococcus acidilactici (Pediococcus acidilactici) NR in any one of the following: (1) Preparing a wheat bran fermenting agent by combining with the compound enzyme preparation; (2) The protein content in the fermented wheat bran is improved; (3) Preparing a preparation for increasing the protein content in the fermented wheat bran; the preservation number of the pediococcus acidilactici NR is CCTCC NO: M20252979; the complex enzyme preparation comprises cellulase, acid protease, neutral protease, alkaline protease and phytase.
2. 2. A wheat bran leaven is characterized by comprising Pediococcus acidilactici NR and a complex enzyme preparation; the preservation number of the pediococcus acidilactici NR is CCTCC NO: M20252979; the complex enzyme preparation comprises cellulase, acid protease, neutral protease, alkaline protease and phytase.
3. 3. Use of the wheat bran starter according to claim 2 for fermenting wheat bran and/or increasing the protein content of fermented wheat bran.
4. 4. A method for preparing fermented wheat bran and/or increasing the protein content of fermented wheat bran, comprising the step of fermenting wheat bran with the wheat bran starter according to claim 2.
5. 5. The method according to claim 4, comprising the steps of pulverizing wheat bran, adding the NR bacterial liquid of Pediococcus acidilactici and the complex enzyme preparation, and performing anaerobic fermentation at 37 ℃ for 48-96 hours.
6. 6. The method of claim 5, wherein the live bacteria count of the Pediococcus acidilactici NR bacteria solution is 3X 10 9 cfu/mL; The consumption of the Pediococcus acidilactici NR bacterial liquid is 1% of the mass of the wheat bran; In the compound enzyme preparation, the dosage of cellulase is 100U/g dry basis, the dosage of acid protease is 50U/g dry basis, the dosage of neutral protease is 100U/g dry basis, the dosage of alkaline protease is 100U/g dry basis, and the dosage of phytase is 30U/g dry basis.
7. 7. Fermented wheat bran obtainable by the process of any one of claims 4-6.
8. 8. Use of the fermented wheat bran as claimed in claim 7 in the preparation of a feed additive or feed.
9. 9. The use according to claim 8, wherein the fermented wheat bran is capable of replacing part of the corn and/or bean pulp in the feed.
10. 10. A feed additive is characterized in that, the feed additive comprises the fermented wheat bran of claim 7.

Description

Application of Pediococcus acidilactici NR-combined complex enzyme preparation in preparation of wheat bran starter and fermentation method thereof Technical Field The invention relates to the field of fermentation engineering and feed processing, in particular to application of a Pediococcus acidilactici NR-combined complex enzyme preparation in preparing a wheat bran starter and a fermentation method thereof. Background The development of native, economical and efficient protein feed raw materials to partially replace imported soybean meal has become a key problem to be solved urgently in the feed industry. Wheat bran is a byproduct in the wheat processing process, and has potential to be a high-quality protein feed substitute raw material due to the abundant protein content and low price. However, the protein in the wheat bran mainly exists in a combined state, and is tightly combined with structural polysaccharides such as cellulose and hemicellulose to form a complex cross-linked structure, so that the acid-soluble protein content is low, the protein is difficult to release, the digestibility is low during direct feeding, and the animal is difficult to fully absorb and utilize, so that a large amount of nutrient substances are wasted, and the wide application of the wheat bran in feeds is limited. In recent years, the application of the microbial fermentation technology in the field of feed processing provides a new idea for the efficient utilization of wheat bran. How to remarkably improve the digestibility and the absorptivity of the protein, so that the acid-soluble protein content of the fermented wheat bran is remarkably improved, and the digestibility of the protein in vitro is also greatly improved, and the wheat bran becomes an efficient protein feed raw material which is not reported at present. Disclosure of Invention The invention aims to provide an application of Pediococcus acidilactici NR combined with a complex enzyme preparation in preparing a wheat bran starter and a fermentation method thereof, so as to solve the problems of the prior art, and provides a method for improving the protein content and the protein digestibility of fermented wheat bran by synergistic fermentation of Pediococcus acidilactici and a complex enzyme system. In the actual animal feeding test, the fermented wheat bran shows excellent feed substitution effect, can partially replace corn and soybean meal, remarkably improves the feed digestibility and reduces the feed cost. In order to achieve the above object, the present invention provides the following solutions: The invention provides the use of Pediococcus acidilactici (Pediococcus acidilactici) NR in any one of the following: (1) Preparing a wheat bran fermenting agent by combining with the compound enzyme preparation; (2) The protein content in the fermented wheat bran is improved; (3) Preparing a preparation for increasing the protein content in the fermented wheat bran; the preservation number of the pediococcus acidilactici NR is CCTCC NO: M20252979; the complex enzyme preparation comprises cellulase, acid protease, neutral protease, alkaline protease and phytase. The invention also provides a wheat bran leaven which comprises Pediococcus acidilactici NR and a complex enzyme preparation; the preservation number of the pediococcus acidilactici NR is CCTCC NO: M20252979; the complex enzyme preparation comprises cellulase, acid protease, neutral protease, alkaline protease and phytase. The invention also provides application of the wheat bran starter in fermenting wheat bran and/or improving protein content in the fermented wheat bran. The invention also provides a method for preparing the fermented wheat bran and/or increasing the protein content in the fermented wheat bran, which comprises the step of fermenting the wheat bran by using the wheat bran starter. Optionally, the method comprises the steps of crushing wheat bran, adding the NR bacterial liquid of the pediococcus acidilactici and the complex enzyme preparation, and carrying out anaerobic fermentation for 48-96 hours at the temperature of 37 ℃. Optionally, the viable count of the NR bacterial liquid of the pediococcus acidilactici is 3 multiplied by 10 9 cfu/mL; The consumption of the Pediococcus acidilactici NR bacterial liquid is 1% of the mass of the wheat bran; In the compound enzyme preparation, the dosage of cellulase is 100U/g dry basis, the dosage of acid protease is 50U/g dry basis, the dosage of neutral protease is 100U/g dry basis, the dosage of alkaline protease is 100U/g dry basis, and the dosage of phytase is 30U/g dry basis. The invention also provides the fermented wheat bran prepared by the method. The invention also provides application of the fermented wheat bran in preparing feed additives or feeds. Optionally, the fermented wheat bran can replace part of the corn and/or soybean meal in the feed. The invention also provides a feed additive, which comprises the fermented wheat