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CN-122004350-A - Biomass-based acidified microbial enzymatic hydrolysis feed and preparation method thereof

CN122004350ACN 122004350 ACN122004350 ACN 122004350ACN-122004350-A

Abstract

The invention discloses an acidified microbial enzymatic hydrolysis feed based on biomass base and a preparation method thereof, and belongs to the technical field of biological processing. The preparation method comprises the steps of compounding raw materials, carrying out enzymolysis and fermentation, emulsifying grease and mixing and embedding. The preparation method comprises the steps of adopting the compounding of various raw materials, providing various nutritional ingredients and organic acids, converting the nutritional ingredients and the organic acids into micromolecular nutrients which are easier to absorb through enzymolysis, and generating a large amount of organic acids through fermentation enrichment, so that metabolites are enriched, and the nutrition diversification is realized. In the mixing embedding process, the buffer is adopted to pretreat the fermentation slurry, so that the thermal decomposition and volatilization loss of organic acid can be greatly reduced, all effective acid components are reserved, the wall material used for embedding is a mixture of sodium alginate and phosphorylated crosslinked starch, the phosphorylated crosslinked starch can fill the pores of a sodium alginate framework, the compactness of the wall material is improved, the embedding rate is improved, and the targeting property of a feed product is further enhanced by introducing phosphate groups, and the digestion and absorption rate is improved.

Inventors

  • CHENG MENGWANLI
  • CHENG MAOJI
  • XUE QIN
  • GAO HAIFENG
  • JIANG XUESONG
  • ZHU TAOTAO
  • MENG LONG
  • FU XIUDI

Assignees

  • 合肥汉泰生物科技有限公司
  • 安徽汉泰生物工程股份有限公司

Dates

Publication Date
20260512
Application Date
20260202

Claims (10)

  1. 1. The preparation method of the biomass-based acidified microbial enzymatic hydrolysis feed is characterized by comprising the following process steps of: S1, crushing dried citric acid residues and dry-base raw materials, mixing sugar-containing wet-base raw materials, adding water, and pulping to prepare raw material slurry; s2, adding compound enzyme into the raw material slurry, and performing enzymolysis reaction to obtain enzymolysis slurry; S3, adding a composite microbial inoculum into the enzymolysis slurry for fermentation, and filtering to obtain fermentation slurry; s4, adding vegetable oil into 20-30% of fermentation slurry, mixing, adding an emulsifying agent, and homogenizing and emulsifying to obtain emulsified grease; s5, adding a buffering agent into the balance fermentation slurry, adjusting the pH value to be 4.5-5.5, then adding emulsified grease, beneficial bacteria suspension and composite wall material solution, uniformly mixing, and then filtering, concentrating and spray drying to obtain the microbial fermentation slurry.
  2. 2. The preparation method of the biomass-based acidified microbial enzymatic hydrolysis feed, which is characterized in that the mass ratio of the citric acid sludge to the dry-based raw material to the mixed sugar-containing wet-based raw material is (50-70): (15-30): (5-20); The dry-based raw material comprises one or more of soybean meal, rice bran, rapeseed meal and corn starch residues, and the sugar-containing wet-based raw material comprises one or more of corn sugar residues, rice residues, pericarps and distillers' grains.
  3. 3. The preparation method of the biomass-based acidified microbial enzymatic hydrolysis feed, which is disclosed in claim 1, is characterized in that the complex enzyme comprises two or more of protease, amylase, saccharifying enzyme, pectase, cellulase and glucosidase, and the addition amount of the complex enzyme is 100-300U/g.
  4. 4. The preparation method of the biomass-based acidified microbial enzymatic hydrolysis feed, which is disclosed in claim 1, is characterized in that the composite microbial agent comprises aspergillus niger and other microbial agents with the mass ratio of 1 (0.4-0.6), wherein the other microbial agents comprise one or more of lactobacillus, saccharomycetes and bacillus licheniformis, and the addition amount of the composite microbial agent is 0.3-0.5%.
  5. 5. The method for preparing a biomass-based acidified microbial enzymatic feed of claim 1, wherein the buffer comprises one or a combination of two of sodium citrate and sodium bicarbonate.
  6. 6. The preparation method of the biomass-based acidified microbial enzymatic hydrolysis feed, which is characterized in that the mass ratio of the fermentation slurry to the vegetable oil to the composite wall material is (15-20): 5-10): 40-50.
  7. 7. The preparation method of the biomass-based acidified microbial enzymatic hydrolysis feed, which is disclosed in claim 1, is characterized in that the beneficial bacteria suspension comprises 50-60% of beneficial bacteria by mass, the beneficial bacteria comprise one or a combination of two of bacillus subtilis and bacillus coagulans, and the addition amount of the beneficial bacteria is 0.1×10 4 ～0.3×10 4 cfu/g.
  8. 8. The preparation method of biomass-based acidified microbial enzymatic hydrolysis feed, according to claim 1, wherein the composite wall material comprises sodium alginate and phosphorylated cross-linked starch in a mass ratio of (30-50): (50-70); The raw materials of the phosphorylated cross-linked starch comprise high-amylose starch, sodium trimetaphosphate and a phosphorylating agent with the mass ratio of (0.05-0.07) to (0.02-0.04), wherein the phosphorylating agent comprises one or a combination of more of sodium tripolyphosphate, disodium hydrogen phosphate, sodium pyrophosphate and sodium dihydrogen phosphate.
  9. 9. The method for preparing biomass-based acidified microbial enzymatic hydrolysis feed of claim 8, wherein the phosphorylated cross-linked starch is prepared according to the following method: Adding high amylose starch into water, mixing to obtain starch slurry with the mass fraction of 30-35%, adding sodium trimetaphosphate and a phosphorylating reagent, adjusting the pH value to 10-11, increasing the temperature to 40-45 ℃, stirring and reacting for 2-3 h, and finally neutralizing, washing, centrifuging, heat treating and sieving to obtain the modified starch.
  10. 10. The biomass-based acidified microbial enzymatic hydrolysis feed, which is characterized by being prepared by adopting the preparation method of the biomass-based acidified microbial enzymatic hydrolysis feed as claimed in any one of claims 1-9.

Description

Biomass-based acidified microbial enzymatic hydrolysis feed and preparation method thereof Technical Field The invention relates to the technical field of biological processing, in particular to an acidified microbial enzymatic hydrolysis feed based on biomass base and a preparation method thereof. Background With the development of intensive animal husbandry, the search for safe, efficient and sustainable feed additives and functional feed products has a key meaning. The feed is especially suitable for weaned livestock and fowl cubs such as piglets, calves, young birds and the like, and female animals in lactation period, and has the defects of imperfect development of digestive system, insufficient secretion of enzyme system, relatively low immune function and high requirements on nutritional composition and digestive characteristics of feed. At present, many feeds are prepared by adopting food-grade grains, beans and other raw materials through simple processing, and most small-molecule nutrients such as organic acid, sugar, maltodextrin and the like added into the feeds also adopt food-grade products. The products are subjected to refining processes such as decolorization, ion exchange, neutralization, crystallization and the like, and the products have high purity, but natural accompanying nutrition substances such as raw vitamins, mineral elements and the like can be greatly lost in the refining process, and even cereal raw materials which are warm and originally helpful for maintaining the health of the digestive tracts of livestock and poultry are converted into cold after being subjected to high temperature or deep processing, so that weaned piglets with weak spleen and stomach functions are caused to have dyspepsia and abnormal intestinal peristalsis, diarrhea is easily induced, and the problems of growth performance, health and the like are influenced. In order to solve the problem, biological feed rich in small molecular nutrients exists, for example, chinese patent application with patent publication No. CN104522296A discloses a preparation method of instant enzymolysis, fermentation and emulsion feed, and the prepared feed is rich in various small molecular nutrients and is favorable for digestion and absorption, but lacks the combined effect of acidification and nutrition, does not contain citric acid, has very low content of lactic acid, leads to limited integral acidification capacity, has unsatisfactory effect of regulating pH value of intestinal tract and inhibiting pathogenic bacteria, and has poor performance in the aspects of replacing feed antibiotics, effectively preventing and controlling diarrhea and the like. Therefore, a novel feed capable of overcoming the defects is needed to be obtained, the nutrition loss caused by a refining process can be effectively avoided, the active ingredients with various synergistic effects are enriched naturally through a scientific bioconversion process, and the problem of single functional components of the feed is overcome, so that the intestinal health of young livestock can be effectively ensured, the dependence on antibiotics and animal protein sources is reduced, the digestion and absorption are promoted, diarrhea is prevented, and the discharge of excrement is reduced. Disclosure of Invention The invention provides an acidified microbial enzymatic hydrolysis feed based on biomass and a preparation method thereof, which can solve the problems of single nutritional components, incomplete nutritional components, easy induction of diarrhea of young livestock and the like in the feed for animal feeding in the prior art. In a first aspect, the invention provides a preparation method of an acidified microbial enzymatic hydrolysis feed based on biomass base, comprising the following process steps: S1, crushing dried citric acid residues and dry-base raw materials, mixing sugar-containing wet-base raw materials, adding water, and pulping to prepare raw material slurry; s2, adding compound enzyme into the raw material slurry, and performing enzymolysis reaction to obtain enzymolysis slurry; S3, adding a composite microbial inoculum into the enzymolysis slurry for fermentation, and filtering to obtain fermentation slurry; s4, adding vegetable oil into 20-30% of fermentation slurry, mixing, adding an emulsifying agent, and homogenizing and emulsifying to obtain emulsified grease; s5, adding a buffering agent into the balance fermentation slurry, adjusting the pH value to be 4.5-5.5, then adding emulsified grease, beneficial bacteria suspension and composite wall material solution, uniformly mixing, and then filtering, concentrating and spray drying to obtain the microbial fermentation slurry. Preferably, the mass ratio of the citric acid residues to the dry-base raw materials to the mixed sugar-containing wet-base raw materials is (50-70): (15-30): (5-20); The dry-based raw material comprises one or more of soybean meal, rice bran, rapeseed meal and cor