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CN-122004351-A - Enzymolysis-fermentation synergistic biological peptide feed additive and preparation method thereof

CN122004351ACN 122004351 ACN122004351 ACN 122004351ACN-122004351-A

Abstract

The invention discloses an enzymolysis-fermentation synergistic biological peptide feed additive and a preparation method thereof, and relates to the technical field of feed additives, wherein the fermentation wet material comprises 100 parts of rapeseed meal, cellulase, pectase, neutral protease, a composite microbial inoculum, astragalus polysaccharide, xylooligosaccharide, linoleic acid, a traditional Chinese medicine decoction and a proper amount of hydrochloric acid; the composite microbial inoculum is prepared from lactobacillus plantarum, lactobacillus acidophilus and bacillus subtilis. The invention prepares the feed additive by components such as rapeseed meal, cellulase, neutral protease, astragalus polysaccharide and the like, realizes the biological conversion process of enzymolysis-fermentation synergy, solves the problem that a single additive is difficult to simultaneously meet multiple requirements of nutrition, immunity and the like, improves the nutritional value and the utilization efficiency of feed raw materials, and improves the immunity and disease resistance of animals.

Inventors

  • HU ZHIJUN
  • GUO CHUANG

Assignees

  • 江苏富海生物科技有限公司

Dates

Publication Date
20260512
Application Date
20260318

Claims (10)

  1. 1. The enzymolysis-fermentation synergistic biological peptide feed additive is characterized by comprising, by weight, 100 parts of rapeseed meal, 0.06-0.08 part of cellulase, 0.04-0.06 part of pectase, 0.03-0.05 part of neutral protease, 1-3 parts of complex microbial inoculum, 0.5-2 parts of astragalus polysaccharide, 0.1-0.5 part of xylooligosaccharide, 1-3 parts of linoleic acid, 100-110 parts of traditional Chinese medicine decoction and a proper amount of hydrochloric acid; The composite microbial inoculum is prepared from lactobacillus plantarum, lactobacillus acidophilus and bacillus subtilis.
  2. 2. The enzymolysis-fermentation synergistic biological peptide feed additive of claim 1, wherein the preparation method of the fermentation wet material comprises the following steps: Pulverizing rapeseed meal by a pulverizer, sieving with a 40-60 mesh screen, mixing the pulverized and sieved rapeseed meal with cellulase, pectase, neutral protease, astragalus polysaccharide, xylooligosaccharide and linoleic acid, adding the Chinese medicinal decoction, stirring, adding hydrochloric acid, and regulating pH to 6-7 to obtain a mixed material; Placing the mixed materials into a fermentation tank, and preserving heat for 4-6 hours at 45-50 ℃ to obtain enzymolysis materials; Cooling to 30-37 ℃, adding a composite microbial inoculum into the enzymolysis material, stirring, sealing a fermentation tank, and fermenting for 48-72 hours to obtain a fermentation wet material.
  3. 3. The enzymolysis-fermentation synergistic biological peptide feed additive according to claim 1, wherein the ratio of the strains of lactobacillus plantarum, lactobacillus acidophilus and bacillus subtilis is 2:2:3.
  4. 4. The enzymolysis-fermentation synergistic biological peptide feed additive according to claim 1, wherein the nutrient index of the rapeseed meal is that the crude protein content is more than or equal to 36%, the crude fiber content is less than or equal to 12%, the thioglucoside content is less than or equal to 25 mu mol/g, and the moisture content is less than or equal to 12%.
  5. 5. The enzymolysis-fermentation synergistic biological peptide feed additive according to claim 1, wherein the enzyme activity of the cellulase is more than or equal to 1.5X10 5 FPU/g, the pectase is more than or equal to 2X 10 5 U/g, and the neutral protease is more than or equal to 1X 10 5 U/g.
  6. 6. The enzymolysis-fermentation synergistic biological peptide feed additive according to claim 1, wherein the polysaccharide content of astragalus polysaccharide is more than or equal to 70%, and the purity of xylooligosaccharide is more than or equal to 95%.
  7. 7. The enzymolysis-fermentation synergistic biological peptide feed additive of claim 1, wherein the preparation method of the traditional Chinese medicine decoction comprises the following steps: mixing herba Houttuyniae, folium Artemisiae Argyi, glycyrrhrizae radix, semen Vaccariae, herba Potentillae Discoloris, and herba Acalyphae, placing into a container, adding 10 times of distilled water, soaking for 30min, heating the soaked materials to boil, and standing for 30min; collecting the first decoction with gauze, adding 8 times of distilled water into the residue, repeating the above decoction process, and filtering with gauze again; mixing the decoctions, and concentrating under heating to obtain decoction containing crude drug 1 g/ml.
  8. 8. The enzymolysis-fermentation synergistic biological peptide feed additive of claim 7, wherein the mass ratio of herba Houttuyniae, folium Artemisiae Argyi, glycyrrhrizae radix, semen Vaccariae, herba Potentillae Discoloris and herba Acalyphae is 6:4:6:3:3:7.
  9. 9. The preparation method of the enzymolysis-fermentation synergistic biological peptide feed additive is suitable for the enzymolysis-fermentation synergistic biological peptide feed additive according to any one of claims 1 to 8, and is characterized in that the preparation method of the feed additive comprises the following steps: S1, drying a fermentation wet material at 50 ℃ until the moisture content is less than or equal to 12%, crushing the dried material by using a crusher, and sieving the crushed material by using a 40-60 mesh screen to obtain fermentation dry powder; S2, placing the screened fermented dry powder and zeolite powder into a three-dimensional mixer, and mixing for 15-45min to obtain the feed additive.
  10. 10. The method for preparing the enzymolysis-fermentation synergistic biological peptide feed additive according to claim 9, wherein the mass ratio of the fermentation dry powder to the zeolite powder is 1:0.2.

Description

Enzymolysis-fermentation synergistic biological peptide feed additive and preparation method thereof Technical Field The invention relates to the technical field of feed additives, in particular to an enzymolysis-fermentation synergistic biological peptide feed additive and a preparation method thereof. Background In order to ensure the safety and public health of animal foods, the use of feed antibiotics is strictly limited worldwide, and the breeding industry is promoted to demand green feed additives with the functions of promoting growth, preventing diarrhea and the like. Protein materials such as soybean meal are expensive and supply fluctuates. The rapeseed meal contains anti-nutritional factors, limits the application of the rapeseed meal and needs to be degraded and converted into a safe high-value feed raw material. The single enzymolysis technology efficiently degrades protein to generate peptide, but the fiber damage is limited and the product has single function; the method has the advantages that probiotics and organic acid are produced by a single microbial fermentation technology, palatability is improved, but efficiency is greatly influenced by pretreatment, conversion is incomplete and functions are insufficient, precursor substances are provided by enzymolysis through cooperation of enzymolysis and fermentation, and the fermentation is further converted and modified to produce active substances, so that the method meets the development requirements of the current green culture and resource efficient utilization industry. Patent CN102948621B discloses a probiotic peptide biological feed additive, a preparation method and application thereof, and the patent realizes the improvement of animal gastrointestinal microenvironment, has double biological effects of probiotics and active peptide, and improves animal feed intake. The active peptide in the probiotic peptide biological feed additive can stimulate the immune function of animals, improve the immune capability of the animals, solve the problems of high content of bitter peptide and poor palatability caused by the processes of acid, alkali, protease enzymolysis and the like, but still has room for optimization in the aspect of enzymolysis-fermentation synergy. Therefore, the application provides an enzymolysis-fermentation synergistic biological peptide feed additive for realizing the enzymolysis-fermentation synergistic biological conversion process and a preparation method thereof. Disclosure of Invention The invention aims to provide an enzymolysis-fermentation synergistic biological peptide feed additive and a preparation method thereof, so as to solve the technical problem that a single additive provided in the background art is difficult to simultaneously meet multiple requirements of nutrition, immunity and the like. The invention provides a technical scheme that the enzymolysis-fermentation synergistic biological peptide feed additive comprises fermentation wet materials, wherein the fermentation wet materials comprise, by weight, 100 parts of rapeseed meal, 0.06-0.08 part of cellulase, 0.04-0.06 part of pectase, 0.03-0.05 part of neutral protease, 1-3 parts of complex microbial inoculum, 0.5-2 parts of astragalus polysaccharide, 0.1-0.5 part of xylooligosaccharide, 1-3 parts of linoleic acid, 100-110 parts of traditional Chinese medicine decoction and a proper amount of hydrochloric acid; The composite microbial inoculum is prepared from lactobacillus plantarum, lactobacillus acidophilus and bacillus subtilis. Preferably, the preparation method of the fermented wet material comprises the following steps: Pulverizing rapeseed meal by a pulverizer, sieving with a 40-60 mesh screen, mixing the pulverized and sieved rapeseed meal with cellulase, pectase, neutral protease, astragalus polysaccharide, xylooligosaccharide and linoleic acid, adding the Chinese medicinal decoction, stirring, adding hydrochloric acid, and regulating pH to 6-7 to obtain a mixed material; Placing the mixed materials into a fermentation tank, and preserving heat for 4-6 hours at 45-50 ℃ to obtain enzymolysis materials; Cooling to 30-37 ℃, adding a composite microbial inoculum into the enzymolysis material, stirring, sealing a fermentation tank, and fermenting for 48-72 hours to obtain a fermentation wet material. Preferably, the ratio of the lactobacillus plantarum, lactobacillus acidophilus and bacillus subtilis is 2:2:3. Preferably, the nutrient index of the rapeseed meal is that the crude protein content is more than or equal to 36%, the crude fiber content is less than or equal to 12%, the thioglucoside content is less than or equal to 25 mu mol/g, and the moisture content is less than or equal to 12%. Preferably, the enzyme activity of the cellulase is more than or equal to 1.5X10 5 FPU/g, the enzyme activity of the pectase is more than or equal to 2X 10 5 U/g, and the enzyme activity of the neutral protease is more than or equal to 1X 10 5 U/g. Preferably