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CN-122004354-A - A fowl metagenesis preparation prepared by solid fermentation of Chinese medicinal byproduct, and its preparation method

CN122004354ACN 122004354 ACN122004354 ACN 122004354ACN-122004354-A

Abstract

The application relates to the technical field of feed additives, and in particular discloses a poultry metazoan preparation prepared by solid state fermentation of a traditional Chinese medicine byproduct and a preparation method thereof. The preparation method of the poultry metazoan preparation prepared by solid fermentation of the traditional Chinese medicine byproducts comprises the following steps of mixing astragalus by-products, honeysuckle leaf by-products, bean pulp powder and sodium alginate, cooling a hydrothermal modified material, adding compound cellulase, glucose oxidase and compound strains, carrying out three-stage solid fermentation on an inoculated material, mixing the fermented material with modified montmorillonite, and carrying out pulsed electric field treatment inactivation. According to the application, sodium alginate is adopted to form a gel network in hydrothermal pre-modification to protect heat-sensitive active ingredients, and compound cellulase and glucose oxidase are synchronously added to relieve catabolism repression, and modified montmorillonite in-situ adsorption and pulse electric field non-heat inactivation are adopted, so that the stability of functional ingredients in intestinal environments and the storage stability of products are enhanced.

Inventors

  • ZHAO LIN
  • LI KEKE
  • GUO SONGFENG
  • FENG LIFEI

Assignees

  • 河南金百合生物科技股份有限公司

Dates

Publication Date
20260512
Application Date
20260413

Claims (10)

  1. 1. The preparation method of the poultry metazoan preparation prepared by solid state fermentation of the Chinese medicinal byproducts is characterized by comprising the following steps of: Mixing astragalus by-products, honeysuckle leaf by-products, soybean meal and sodium alginate, adding water, and performing hydrothermal pre-modification to obtain a hydrothermal modified material; Cooling the hydrothermal modified material, adding composite cellulase, glucose oxidase and composite strain, and mixing to obtain inoculated material; carrying out three-stage solid state fermentation on the inoculated material to obtain a fermented material; Mixing the fermented material with modified montmorillonite, and performing pulsed electric field treatment for inactivation to obtain an inactivated material; drying the inactivated material at low temperature and pulverizing to obtain the metazoan preparation for poultry.
  2. 2. The method for preparing the poultry metazoan preparation prepared by solid-state fermentation of the Chinese medicinal byproduct according to claim 1, wherein the solid-to-liquid ratio of the hydrothermal pre-modification after adding water is 1:0.7-0.9, the temperature is 120-140 ℃, the pressure is 0.2-0.3 MPa, and the heat preservation time is 30-45 min.
  3. 3. The preparation method of the poultry metazoan preparation prepared by solid-state fermentation of traditional Chinese medicine byproducts according to claim 1 is characterized in that the temperature after cooling is 25-35 ℃, the addition amount of the compound cellulase is 15-25 FPU/g dry basis, the addition amount of the glucose oxidase is 5-10U/g dry basis, the compound strain is prepared by mixing bacillus subtilis, lactobacillus acidophilus and aspergillus niger according to the viable bacteria number ratio of 2-4:1:1-3, the total inoculation amount is 5 multiplied by 10 7 CFU/g dry basis, the stirring speed for mixing is 20-50 r/min, and the stirring time is 5-15 min.
  4. 4. The method for preparing the poultry metazoan preparation prepared by solid state fermentation of the Chinese medicinal byproduct according to claim 1, wherein the three-stage solid state fermentation comprises the following steps of: The inoculation materials are subjected to forced ventilation at the temperature of 30-32 ℃ and the ventilation ratio of 0.3-0.7 vvm, the relative humidity is 85% -90%, and the treatment is carried out for 24 hours; Then anaerobic pulse is carried out every 3-5 hours at the temperature of 35-37 ℃, CO 2 is introduced during the pulse period, the flow is 0.1-0.3 vvm, the duration is 3-8 min, the pile is turned over and the treatment is carried out for 24 hours; And then, at the temperature of 35-42 ℃, completely sealing, continuously introducing CO 2 or N 2 with the flow of 0.05-0.15 vvm, and treating for 24 hours to obtain the fermentation material.
  5. 5. The method for preparing the poultry metazoan preparation prepared by solid-state fermentation of Chinese medicinal byproducts according to claim 1, wherein the field strength of the pulsed electric field treatment inactivation is 15-25 kV/cm, the pulse width is 20-50 mu s, the pulse frequency is 10-30 Hz, the treatment time is 5-15 min, the material thickness is less than or equal to 2cm, and the material temperature during the treatment is less than or equal to 45 ℃.
  6. 6. The method for preparing the poultry metazoan preparation prepared by solid-state fermentation of the Chinese medicinal byproduct according to claim 1, wherein the low-temperature drying temperature is 50-60 ℃, the vacuum degree is-0.08 to-0.09 MPa, the water content is less than or equal to 10%, and the crushed particle size is 60-100 meshes.
  7. 7. The method for preparing the poultry metazoan preparation prepared by solid-state fermentation of Chinese medicinal byproducts according to claim 1, wherein the astragalus root byproducts are obtained by extracting astragalus root with alcohol and then drying, the water content is less than or equal to 15%, and the honeysuckle leaf byproducts are obtained by extracting honeysuckle leaves with water and then drying, and the water content is less than or equal to 15%.
  8. 8. The method for preparing the poultry metazoan preparation prepared by solid state fermentation of Chinese medicinal byproducts according to claim 1, wherein the viscosity of the sodium alginate is 200-400 mPa.s, the modified montmorillonite is sodium-based activated montmorillonite, the cation exchange capacity is more than or equal to 90mmol/100g, and the particle size is less than or equal to 200 meshes.
  9. 9. The preparation method of the poultry metazoan preparation prepared by solid state fermentation of traditional Chinese medicine byproducts according to claim 1 is characterized in that the weight parts of astragalus by-products, honeysuckle leaf by-products, bean pulp powder and sodium alginate when being mixed are 40-60 parts of astragalus by-products, 20-30 parts of honeysuckle leaf by-products, 10-15 parts of bean pulp powder and 0.5-1.5 parts of sodium alginate, and the addition amount of the modified montmorillonite is 5-8 parts by weight.
  10. 10. A preparation of a post-natal preparation for poultry prepared by solid state fermentation of Chinese herbal byproducts, which is characterized by being prepared by the preparation method of any one of claims 1 to 9.

Description

A fowl metagenesis preparation prepared by solid fermentation of Chinese medicinal byproduct, and its preparation method Technical Field The application relates to the technical field of feed additives, in particular to a poultry metazoan preparation prepared by solid fermentation of a traditional Chinese medicine byproduct and a preparation method thereof. Background Along with the large-scale and intensive development of livestock and poultry breeding industry, the intestinal health problem of poultry is increasingly focused, and metazoan is taken as the general name of inactivated thalli of probiotics and metabolites thereof, has the effects of regulating intestinal flora, enhancing immune function, improving the integrity of intestinal barriers and the like, has the advantages of good storage stability, high temperature resistance of feed pelleting, no ecological risk of releasing viable bacteria and the like compared with a viable bacteria preparation, becomes a research hotspot in the field of feed additives, and is widely applied to the preparation of feed additives due to the characteristics of low energy consumption, less waste water emission, wide culture substrate sources and the like. The existing metazoan preparation for poultry is prepared by mixing auxiliary materials such as bran, bean pulp and the like, then performing steam sterilization, inoculating microorganisms such as bacillus subtilis and the like, performing solid-state fermentation under natural ventilation condition, and then drying and crushing by hot air, but the metabolic requirements of aerobic bacteria and anaerobic bacteria are difficult to consider in the fermentation process, metabolic conflict is easy to occur, the directional enrichment of functional components is influenced, and the functional effect of the product is reduced. Disclosure of Invention The application provides a poultry metazoan preparation prepared by solid fermentation of traditional Chinese medicine byproducts and a preparation method thereof, aiming at solving the problems that the existing poultry metazoan preparation is easy to generate metabolic conflict, affects the directional enrichment of functional components and leads to the reduction of the functional effect of products. The application provides a poultry metazoan preparation prepared by solid fermentation of Chinese medicinal byproducts and a preparation method thereof, which adopts the following technical scheme: in a first aspect, the application provides a preparation method of a poultry metazoan preparation prepared by solid state fermentation of a traditional Chinese medicine byproduct, which adopts the following technical scheme: the preparation method of the poultry metazoan preparation prepared by solid state fermentation of the Chinese medicinal byproducts comprises the following steps of: Mixing astragalus by-products, honeysuckle leaf by-products, soybean meal and sodium alginate, adding water, and performing hydrothermal pre-modification to obtain a hydrothermal modified material; Cooling the hydrothermal modified material, adding composite cellulase, glucose oxidase and composite strain, and mixing to obtain inoculated material; carrying out three-stage solid state fermentation on the inoculated material to obtain a fermented material; Mixing the fermented material with modified montmorillonite, and performing pulsed electric field treatment for inactivation to obtain an inactivated material; drying the inactivated material at low temperature and pulverizing to obtain the metazoan preparation for poultry. By adopting the technical scheme, as the sodium alginate is adopted to form a gel network in hydrothermal pre-modification to protect thermosensitive active ingredients, and compound cellulase and glucose oxidase are synchronously added to relieve catabolic repression, and meanwhile, three-section solid state fermentation containing anaerobic pulses is adopted to realize stable transition of flora metabolism, and modified montmorillonite is adopted to perform in-situ adsorption and non-thermal inactivation of a pulse electric field, the enrichment of short-chain fatty acid and extracellular polysaccharide can be improved, the generation of biogenic amine is inhibited, the stability of functional ingredients in an intestinal environment and the storage stability of products are enhanced, and the problems that the existing post-production preparation for poultry is easy to generate metabolic conflict, the directional enrichment of the functional ingredients is influenced, and the functional effect of the products is reduced are solved. Preferably, the solid-liquid ratio of the added water subjected to hydrothermal pre-modification is 1:0.7-0.9, the temperature is 120-140 ℃, the pressure is 0.2-0.3 MPa, and the heat preservation time is 30-45 min. By adopting the technical scheme, the gel network formed by sodium alginate can wrap heat-sensitive components such as astragalus poly