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CN-122004357-A - Preparation method of nano protein selenium for animal feed additive

CN122004357ACN 122004357 ACN122004357 ACN 122004357ACN-122004357-A

Abstract

The invention relates to the technical field of feed additives, and discloses a preparation method of nano-protein selenium for animal feed additives, which comprises the following steps of preparing a protein solution with a mass volume concentration of 1-5%, sequentially adding sodium selenite and soluble salt into the protein solution, stirring and mixing, placing the mixed solution in a 10-50kHz alternating magnetic field generator, controlling the temperature of the solution to be increased to 55-70 ℃ at a rate of 0.5-1.5 ℃ per minute, keeping the temperature for 20-60 minutes, obtaining a solid product through centrifugal separation, washing and freeze-drying to obtain nano-protein selenium composite particles.

Inventors

  • XU CHAOHUI
  • XU YUNING

Assignees

  • 重庆嘉禾瑞宁生物科技有限公司

Dates

Publication Date
20260512
Application Date
20260209

Claims (10)

  1. 1. A method for preparing nano-protein selenium for animal feed additives, which is characterized by comprising the following steps: firstly, dissolving natural protein in phosphate buffer solution to prepare a protein solution with the mass volume concentration of 1-5%; Sequentially adding sodium selenite and soluble salt into the protein solution, and stirring and mixing; Step three, placing the mixed solution in an alternating magnetic field generator with the frequency of 10-50kHz, controlling the temperature of the solution to be raised to 55-70 ℃ at the speed of 0.5-1.5 ℃ per minute, and carrying out heat preservation reaction for 20-60 minutes at the temperature; In the reaction process, the natural protein is subjected to conformational transition and phase separation under the double induction of alternating magnetic field and heat treatment to form nanoscale protein aggregates, selenite ions in the solution are synchronously reduced to zero-valent selenium nanocrystals in situ, and the selenium nanocrystals are subjected to multistage assembly with the protein aggregates and zinc ions or/and copper ions in the solution; And step four, after the reaction is finished, cooling to below 5 ℃, obtaining a solid product through centrifugal separation, and obtaining the nano-protein selenium composite particles through washing and freeze drying.
  2. 2. The method of claim 1, wherein in the first step, the natural protein is one of soy protein isolate and whey protein.
  3. 3. The method for preparing nano-protein selenium for animal feed additives according to claim 1, wherein in the second step, the sodium selenite is added in an amount such that the concentration of selenium element in the protein solution is 0.5-5 mmol/L.
  4. 4. The method for preparing nano-protein selenium for animal feed additives according to claim 1, wherein in the second step, the soluble zinc salts are zinc sulfate heptahydrate and copper sulfate pentahydrate; wherein, the mol ratio of zinc to selenium is 10:1, and the mol ratio of copper to selenium is 2:1.
  5. 5. The method for preparing nano-protein selenium for animal feed additives according to claim 1, wherein in the third step, pectin accounting for 5-15% of the mass of the natural protein is additionally added into the protein solution, and the pectin, the natural protein, sodium selenite and soluble zinc salt participate in the assembling process of the third step together to form a stable polysaccharide-protein-mineral composite network structure.
  6. 6. The method for preparing nano-protein selenium for animal feed additives according to claim 1, wherein in the third step, after the reaction is completed, stirring and temperature are maintained, acrylic acid and N-isopropyl acrylamide are added, and an initiator potassium persulfate is added, the reaction is continued for 40-60 minutes, and a hydrogel polymer layer with dual response of temperature and pH is formed on the surface of the nano-composite mineral particles in situ; the hydrogel polymer layer swells at an environment below 35 ℃ and a pH above 6.5 and shrinks at an environment above 40 ℃ or/and a pH below 5.0.
  7. 7. The method for preparing nano-protein selenium for animal feed additives according to claim 6, wherein the mass ratio of the acrylic acid to the N-isopropyl acrylamide is 1:2-4; The total addition amount of the acrylic acid and the N-isopropyl acrylamide is 10-30% of the mass of the natural protein; the addition amount of the potassium persulfate is 1.0 percent of the total mass of the acrylic acid and the N-isopropyl acrylamide.
  8. 8. The method for preparing nano-protein selenium for animal feed additives according to claim 1, wherein in the fourth step, before freeze-drying, the method further comprises the steps of re-dispersing the washed precipitate in sodium alginate solution, stirring uniformly, then dripping into calcium chloride solution to form sodium alginate-calcium chloride gel microspheres embedded with the nano-composite mineral particles, washing, and freeze-drying.
  9. 9. The method for preparing nano-protein selenium for animal feed additives according to claim 8, wherein the mass concentration of the sodium alginate solution is 1-2%, and the mass concentration of the calcium chloride solution is 2-4%.
  10. 10. A nano-protein selenium for animal feed additives, characterized in that the nano-protein selenium is prepared by the preparation method of the nano-protein selenium for animal feed additives according to any one of claims 1-9, in particular to nano-protein selenium composite particles.

Description

Preparation method of nano protein selenium for animal feed additive Technical Field The invention relates to the technical field of feed additives, in particular to a preparation method of nano protein selenium for an animal feed additive. Background In the current feed additive, selenium is taken as an important trace element, and plays an important role in the aspects of growth and development, immune function, reproductive performance and the like of animals. However, the traditional selenium additive forms, such as inorganic selenium such as sodium selenite, have the problem of low bioavailability although having a certain selenium supplementing effect, and in recent years, along with the continuous development of nano technology, nano selenium gradually becomes a research hot spot in the field of selenium additives due to the unique physicochemical properties and excellent biological activity of the nano selenium. But has the defects of uneven particles, easy agglomeration, poor stability and the like. In many nano-selenium preparation methods, nano-protein selenium is widely focused because of the combination of the nutritional value of proteins and the biological activity of nano-selenium. The protein is used as an important nutrient substance in organisms, has good biocompatibility and degradability, can be used as a carrier of nano selenium, and improves the stability and bioavailability of the nano selenium. Therefore, the application provides a preparation method of nano protein selenium for animal feed additives. Disclosure of Invention The invention aims to provide a preparation method of nano-protein selenium for animal feed additives, which aims to solve the problems in the background art. In a first aspect, in order to solve the above problems, the present invention provides a method for preparing nano-protein selenium for animal feed additives, comprising the steps of: firstly, dissolving natural protein in phosphate buffer solution to prepare a protein solution with the mass volume concentration of 1-5%; Sequentially adding sodium selenite and soluble salt into the protein solution, and stirring and mixing; Step three, placing the mixed solution in an alternating magnetic field generator with the frequency of 10-50kHz, controlling the temperature of the solution to be raised to 55-70 ℃ at the speed of 0.5-1.5 ℃ per minute, and carrying out heat preservation reaction for 20-60 minutes at the temperature; In the reaction process, the natural protein is subjected to conformational transition and phase separation under the double induction of alternating magnetic field and heat treatment to form nanoscale protein aggregates, selenite ions in the solution are synchronously reduced to zero-valent selenium nanocrystals in situ, and the selenium nanocrystals are subjected to multistage assembly with the protein aggregates and zinc ions or/and copper ions in the solution; And step four, after the reaction is finished, cooling to below 5 ℃, obtaining a solid product through centrifugal separation, and obtaining the nano-protein selenium composite particles through washing and freeze drying. Specifically, in the first step, the natural protein is one of soy protein isolate and whey protein. Specifically, in the second step, the adding amount of the sodium selenite enables the concentration of selenium element in the protein solution to be 0.5-5 mmol/L. Specifically, in the second step, the soluble zinc salt is zinc sulfate heptahydrate and copper sulfate pentahydrate; wherein, the mol ratio of zinc to selenium is 10:1, and the mol ratio of copper to selenium is 2:1. Specifically, in the third step, pectin accounting for 5-15% of the mass of the natural protein is additionally added into the protein solution, and the pectin, the natural protein, sodium selenite and soluble zinc salt participate in the assembly process of the third step together to form a stable polysaccharide-protein-mineral composite network structure. Specifically, in the third step, after the reaction is completed, stirring and temperature are kept, acrylic acid, N-isopropyl acrylamide and an initiator potassium persulfate are added, the reaction is continued for 40-60 minutes, and a hydrogel polymer layer with dual response of temperature and pH is formed on the surface of the nano composite mineral particles in situ; the hydrogel polymer layer swells at an environment below 35 ℃ and a pH above 6.5 and shrinks at an environment above 40 ℃ or/and a pH below 5.0. Specifically, the mass ratio of the acrylic acid to the N-isopropyl acrylamide is 1:2-4; The total addition amount of the acrylic acid and the N-isopropyl acrylamide is 10-30% of the mass of the natural protein; the addition amount of the potassium persulfate is 1.0 percent of the total mass of the acrylic acid and the N-isopropyl acrylamide. In the fourth step, before freeze drying, the method further comprises the steps of re-dispersing the washed precipitate in sodium a