Search

CN-122004360-A - Natural plant feed raw material capable of replacing aureomycin and preparation method thereof

CN122004360ACN 122004360 ACN122004360 ACN 122004360ACN-122004360-A

Abstract

The invention belongs to the technical field of animal feed, and in particular relates to a natural plant feed raw material capable of replacing aureomycin and a preparation method thereof, wherein the natural plant feed raw material comprises, by weight, 15-25 parts of astragalus mongholicus, 10-20 parts of hawthorn, 10-18 parts of sea buckthorn, 8-15 parts of schisandra chinensis, 8-15 parts of purslane, 6-12 parts of honeysuckle, 10-18 parts of codonopsis pilosula and 6-12 parts of macleaya cordata. The natural plant feed raw material is a pure traditional Chinese medicine/natural plant source, is free of antibiotics and chemical additives, is used for replacing aureomycin to be used as a feed additive or premix for fattening monogastric/ruminant animals such as pigs, cattle, sheep, horses and the like, is prepared by adopting an integrated innovative process of directional enzymolysis pretreatment, ultrasonic-microwave synergistic extraction, green entrainer modified supercritical CO2 extraction, liposome-nano composite embedding, realizes directional enrichment, efficient extraction and intestinal targeted release of active ingredients, and improves the extraction rate of the active ingredients compared with the traditional process.

Inventors

  • ZHANG KAI
  • YANG CHUNLEI
  • MENG LIXIANG
  • Xin Lihai
  • JIA JINYU

Assignees

  • 山西朗新生物科技有限公司

Dates

Publication Date
20260512
Application Date
20260330

Claims (10)

  1. 1. The preparation method of the natural plant feed raw material capable of replacing aureomycin is characterized by comprising the following steps of: (1) Adding a compound enzyme preparation with the mass percentage of 0.3-0.8% into the raw materials, adjusting the feed-liquid ratio to be 1:5-7 and the pH value to be 5.5-6.5, carrying out enzymolysis at the constant temperature of 45-55 ℃, inactivating in water bath after the enzymolysis is finished, and then adopting vacuum freeze drying for standby; (2) Mixing the raw materials after enzymolysis according to parts by weight, adding an ethanol aqueous solution, extracting and filtering by adopting an ultrasonic-microwave synergistic technology, and collecting primary extract and filter residues for later use; (3) Placing the filter residue obtained in the step (2) into a supercritical CO 2 extraction kettle, adding a green entrainer, extracting and collecting an extract, and purifying the extract by adopting a molecular distillation technology to obtain a purified extract; (4) Mixing the primary extract of the step (2) with the purified extract of the step (3), and concentrating by adopting a reduced pressure concentration technology to obtain a composite extract; (5) Mixing the composite extract with a composite embedding agent according to the mass ratio of 1:2-4, adding a proper amount of deionized water for homogenization to obtain liposome-nano composite embedding liquid; (6) Drying the liposome-nano composite embedding liquid and crushing to obtain the natural plant feed raw material.
  2. 2. The method for preparing the natural plant feed raw material capable of replacing aureomycin according to claim 1, wherein the compound enzyme preparation is a compound system of cellulase, pectase and xylanase, and the mass ratio is 2:1:1.
  3. 3. The method for preparing the natural plant feed raw material capable of replacing aureomycin according to claim 1, wherein in the step (2), the ultrasonic power of the ultrasonic-microwave synergistic technology is 150-250W, the microwave power is 300-500W, the temperature is 60-70 ℃, the extraction is 40-60min, and the ultrasonic-microwave synergistic extraction technology adopts an intermittent operation mode.
  4. 4. The method for preparing the natural plant feed raw material capable of replacing aureomycin according to claim 1, wherein the addition amount of the green entrainer is 3% -5% of the total mass of the raw material.
  5. 5. The method for preparing the natural plant feed raw material capable of replacing aureomycin according to claim 4, wherein the green entrainer is a compound system of thyme essential oil and ethanol, and the mass ratio is 1:3.
  6. 6. The natural plant feed material capable of replacing aureomycin as claimed in claim 5, wherein the extraction pressure in the step (3) is 30-40MPa, the extraction temperature is 40-50℃, CO 2 , the flow rate is 20-30L/h, and the extraction time is 1.5-2.5h.
  7. 7. The method for preparing the natural plant feed raw material capable of replacing aureomycin, which is disclosed in claim 1, is characterized in that the enzymolysis parameters in the step (1) are that the addition amount of the compound enzyme preparation is 0.5% of the mass of the raw material, the feed liquid ratio is 1:6, the pH value is 6.0, the enzymolysis temperature is 50 ℃, the enzymolysis time is 75min, the inactivation time after enzymolysis is 10min, and the vacuum freeze drying is carried out until the water content is 7%.
  8. 8. The method for preparing natural plant feed raw materials capable of replacing aureomycin according to claim 6, wherein the addition amount of the green entrainer in the step (3) is 4% of the total mass of the mixed plant raw materials, the extraction pressure is 35MPa, the extraction temperature is 45℃, CO 2 flow rate 25L/h, and the extraction time is 2h.
  9. 9. The method for preparing the natural plant feed raw material capable of replacing aureomycin according to claim 1, wherein the composite embedding agent is a compound system of beta-cyclodextrin, arabic gum and soybean lecithin, and the mass ratio is 1:1:0.5.
  10. 10. The natural plant feed raw material capable of replacing aureomycin is prepared by the preparation method according to any one of claims 1-9, and is characterized by comprising, by weight, 15-25 parts of astragalus mongholicus, 10-20 parts of hawthorn, 10-18 parts of sea buckthorn, 8-15 parts of schisandra chinensis, 8-15 parts of purslane, 6-12 parts of honeysuckle, 10-18 parts of radix codonopsis and 6-12 parts of macleaya cordata.

Description

Natural plant feed raw material capable of replacing aureomycin and preparation method thereof Technical Field The invention belongs to the technical field of animal feeds, and particularly relates to a natural plant feed raw material capable of replacing aureomycin and a preparation method thereof. Background The aureomycin is used as tetracycline antibiotics and is used as a feed additive for fattening livestock and poultry for a long time, and can reduce intestinal diseases and improve the growth efficiency through bacteriostasis, but the aureomycin is easy to produce drug-resistant strains after long-term use, so that the antibiotic residues in animals are caused, the health of human bodies is influenced through food chains, and meanwhile, the aureomycin also can cause environmental pollution of soil, water bodies and the like, and does not conform to the industry development trend of the current feed forbidden and green cultivation and also does not conform to the national food safety strategic requirements. The conventional tiepin product in the market and related patents have obvious defects that most of the tiepin product is single plant extract, has single function and is difficult to comprehensively replace the antibacterial and growth-promoting dual effects of aureomycin, the preparation process is limited to the traditional ethanol reflux extraction, the problems of inaccurate extraction of active ingredients, poor embedding stability, easy degradation of active ingredients and the like exist, and the plant active ingredients are easy to be degraded by gastric acid and digestive enzymes in gastrointestinal tracts of animals, and have low bioavailability and unstable tiepin effect. The directional enzymolysis technology can specifically decompose cellulose, pectin and other components according to the plant cell wall structure to realize the directional release of active components, is more efficient and more accurate compared with the simple physical crushing, and therefore, the improvement designs a natural plant feed raw material capable of replacing aureomycin and a preparation method thereof. Disclosure of Invention Aiming at the defects in the prior art, the invention provides a natural plant feed raw material capable of replacing aureomycin and a preparation method thereof, which are used for solving the problems in the prior art. In order to solve the technical problems, the invention adopts the following technical scheme: The invention provides a natural plant feed raw material capable of replacing aureomycin, which comprises, by weight, 15-25 parts of astragalus mongholicus, 10-20 parts of hawthorn, 10-18 parts of sea buckthorn, 8-15 parts of schisandra chinensis, 8-15 parts of purslane, 6-12 parts of honeysuckle, 10-18 parts of codonopsis pilosula and 6-12 parts of macleaya cordata. The natural plant feed raw material is pure traditional Chinese medicine/natural plant source, has no antibiotics and no chemical additive, is used for replacing aureomycin as a feed additive or premix for fattening monogastric/ruminant animals such as pigs, cattle, sheep, horses and the like, does not replace basic feed, is prepared by adopting an integrated innovative process of directional enzymolysis pretreatment-ultrasonic-microwave synergistic extraction-green entrainer modified supercritical CO 2 extraction-liposome-nano composite embedding, and realizes directional enrichment, efficient extraction and intestinal targeted release of active ingredients, and the extraction rate of the active ingredients is improved compared with that of the traditional process. The invention also provides a preparation method of the natural plant feed raw material capable of replacing aureomycin, which comprises the following steps: (1) Adding a compound enzyme preparation with the mass percentage of 0.3-0.8% into the raw materials, adjusting the feed-liquid ratio to be 1:5-7 and the pH value to be 5.5-6.5, carrying out enzymolysis at the constant temperature of 45-55 ℃, inactivating in water bath after the enzymolysis is finished, and then adopting vacuum freeze drying for standby; (2) Mixing the raw materials after enzymolysis according to parts by weight, adding an ethanol aqueous solution, extracting and filtering by adopting an ultrasonic-microwave synergistic technology, and collecting primary extract and filter residues for later use; (3) Placing the filter residue obtained in the step (2) into a supercritical CO 2 extraction kettle, adding a green entrainer, extracting and collecting an extract, and purifying the extract by adopting a molecular distillation technology to obtain a purified extract; (4) Mixing the primary extract of the step (2) with the purified extract of the step (3), and concentrating by adopting a reduced pressure concentration technology to obtain a composite extract; (5) Mixing the composite extract with a composite embedding agent according to the mass ratio of 1:2-4, adding a proper amou