CN-122004361-A - Bullfrog feed composition added with vitamin A and application thereof

CN122004361ACN 122004361 ACN122004361 ACN 122004361ACN-122004361-A

Abstract

The application provides a bullfrog feed composition added with vitamin A and application thereof. The bullfrog feed composition comprises 40-60% of protein source, 30-40% of carbon water source, 6-12% of fat source and the balance of additive, wherein in the additive, mineral supplement accounts for 1-7% of total weight of the bullfrog feed composition, vitamin supplement accounts for 0.1-5% of total weight of the bullfrog feed composition, and the vitamin A level of the bullfrog feed composition is 2000-16000 IU/kg. The bullfrog feed composition is used for bullfrog cultivation, can accelerate growth and metamorphosis development of bullfrog tadpoles under the premise of guaranteeing basic nutrition supply and health maintenance, shortens cultivation period, and improves seedling emergence uniformity and yield, thereby improving overall production efficiency and economic benefit, and has definite practical value and wide popularization prospect.

Inventors

ZHANG CHUNXIAO
WANG LING
LU KANGLE
LI XUESHAN
SONG KAI
MA RUIJUAN
GAO JUAN

Assignees

集美大学

Dates

Publication Date: 20260512
Application Date: 20260309

Claims (10)

1. The bullfrog feed composition is characterized by comprising the following components in percentage by weight: 40-60% of protein source, 30-40% of carbon source, 6-12% of fat source and the balance of additive, wherein in the additive, mineral supplement accounts for 1-7% of total weight of the bullfrog feed composition, vitamin supplement accounts for 0.1-5% of total weight of the bullfrog feed composition, and the vitamin A level of the bullfrog feed composition is 2000-16000 IU/kg.
2. The bullfrog feed composition according to claim 1, wherein the protein source is selected from any one of animal protein material, vegetable protein material, and combinations thereof; Preferably, the protein source in the bullfrog feed composition comprises fish meal, corn protein powder and soybean protein concentrate, wherein the fish meal is preferably any one of Engraulis japonicus Temminck et Schlegel powder, chai Yufen, ba Sha Yufen, sardine powder, tuna powder and a combination thereof, the content of the fish meal in the bullfrog feed composition is preferably 10-50%, more preferably 30%, the content of the corn protein powder in the bullfrog feed composition is preferably 1-20%, more preferably 10%, and the content of the soybean protein concentrate in the bullfrog feed composition is preferably 1-20%, more preferably 10%.
3. The bullfrog feed composition according to claim 1, wherein the carbon water source is selected from any one of corn flour, wheat bran, brown rice flour and combinations thereof, preferably high gluten flour.
4. The bullfrog feed composition according to claim 1, wherein the fat source is selected from any one of vegetable oil, animal fat, lecithin, and combinations thereof; Preferably, the fat source components in the bullfrog feed composition comprise soybean oil and lecithin, wherein the content of the soybean oil in the bullfrog feed composition is preferably 1-10%, more preferably 4.3%, and the content of the lecithin in the bullfrog feed composition is preferably 1-10%, more preferably 4.3%.
5. The bullfrog feed composition according to claim 1, wherein the mineral supplement contains an inorganic salt providing mineral elements, optionally together with a carrier, preferably the inorganic salt is selected from any one of calcium salt, phosphorus salt, potassium salt, sodium salt, magnesium salt, iron salt, zinc salt, copper salt, manganese salt, cobalt salt, iodine salt, selenium salt and combinations thereof; Preferably, the bullfrog feed composition comprises a multi-mineral premix, calcium dihydrogen phosphate and calcium carbonate, wherein the content of the multi-mineral premix in the bullfrog feed composition is preferably 0.1-1%, more preferably 0.5%, the content of the calcium dihydrogen phosphate in the bullfrog feed composition is preferably 1-5%, more preferably 3.3%, the content of the calcium carbonate in the bullfrog feed composition is preferably 1-5%, more preferably 2.3%, and the multi-mineral premix comprises potassium chloride, potassium iodide, cobalt sulfate, copper sulfate, ferrous sulfate monohydrate, zinc sulfate monohydrate, manganese sulfate monohydrate, magnesium sulfate heptahydrate, sodium selenite and zeolite powder, and the multi-mineral premix comprises preferably 100-300 parts by weight of potassium chloride, 20-100 parts by weight of potassium iodide, 50-150 parts by weight of cobalt sulfate, 10-40 parts by weight of copper sulfate, 300-500 parts by weight of ferrous sulfate monohydrate, 100-300 parts by weight of zinc sulfate monohydrate, 50-100 parts by weight of magnesium sulfate monohydrate, 4000 parts by weight of magnesium sulfate heptahydrate and 2000-80 parts by weight of water of sodium sulfate heptahydrate.
6. The bullfrog feed composition according to claim 1, characterized in that the vitamin supplement contains vitamins selected from any one of vitamin a, vitamin D, vitamin E, vitamin K, vitamins C, B vitamins and combinations thereof, optionally together with antioxidants and/or carriers, the carrier in the vitamin supplement preferably being flour, the antioxidants preferably being ethoxyquinoline; Preferably, the vitamin supplement composition in the bullfrog feed composition comprises a multi-vitamin premix, choline chloride, vitamin C and vitamin A; wherein the content of the multidimensional premix in the bullfrog feed composition is preferably 0.1-1%, more preferably 0.2%; the content of choline chloride in the bullfrog feed composition is preferably 0.1-1%, more preferably 0.5%, the content of vitamin C in the bullfrog feed composition is preferably 0.1-1%, more preferably 0.1%, the content of vitamin A in the bullfrog feed composition is preferably 0.0001-0.01%, more preferably 0.0006-0.0049%, wherein the multi-vitamin premix composition comprises vitamin B1, riboflavin, pyridoxine hydrochloride, vitamin B12, vitamin K3, inositol, calcium pantothenate, niacin, folic acid, biotin, vitamin D3, vitamin E, ethoxyquinoline, flour, preferably the multi-vitamin premix composition comprises 5-15 parts by weight of vitamin B1, 5-15 parts by weight of riboflavin, 5-15 parts by weight of pyridoxine hydrochloride, 0.1-0.5 parts by weight of vitamin B12, 5-15 parts by weight of vitamin K3, 50-150 parts by weight of inositol, 10-30 parts by weight of vitamin D3, vitamin E, 200-120 parts by weight of vitamin D, 200-200 parts of folic acid, 5-80 parts by weight of vitamin E, 200 parts of vitamin E, 120 parts by weight of vitamin D, 200 parts of vitamin E; Preferably, the vitamin A is selected from any one of retinol, retinol propionate, retinol acetate, retinol palmitate, and combinations thereof, more preferably, the vitamin A is retinol acetate.
7. The bullfrog feed composition according to claim 1, wherein the bullfrog feed composition has a vitamin a level of 2360-4160 IU/kg; optionally, the bullfrog feed composition has a protein level of 38-50%; Optionally, the bullfrog feed composition has a fat level of 7-12%.
8. A bullfrog feed characterized in that the bullfrog feed composition according to claim 1 is used as a dry matter, and the bullfrog feed is preferably obtained by the following preparation method: a) The bullfrog feed composition of claim 1 wherein the raw materials are weighed and all but the fat source raw materials are mixed; b) Adding water into the mixture obtained in the step a) to carry out tempering; c) Puffing and granulating the quenched and tempered material; d) Drying the puffed granules; e) And applying a fat source raw material to the surface of the particles to obtain the bullfrog feed.
9. Use of the bullfrog feed of claim 1 in bullfrog farming.
10. Use of the bullfrog feed according to claim 9 in bullfrog farming, wherein the bullfrog feed is for feeding bullfrog tadpoles; preferably, the daily feeding amount of the bullfrog feed is 5-8%, preferably 6.5% of the weight of the tadpoles.

Description

Bullfrog feed composition added with vitamin A and application thereof Technical Field The invention belongs to the field of animal nutrition and feed science, in particular to a bullfrog feed composition and application thereof, and particularly relates to a bullfrog feed composition with a finely regulated vitamin A level and a method for promoting growth and metamorphosis development of bullfrog tadpoles. Background Bullfrog is an important economic breeding frog, and the breeding period of the bullfrog directly influences the economic benefit. In the bullfrog cultivation period, the synchronicity and uniformity of the tadpole stage growth speed and metamorphosis development are key to determining the whole cultivation efficiency. Tadpole metamorphosis is delayed, and cycle elongation can increase culture cost and risk, and can cause uneven specifications of adult frogs, thereby influencing market income. At present, comprehensive strategies such as optimizing the protein level of feed, adding compound vitamins and functional additives and the like are generally adopted in the industry to promote the growth and development of frogs. For example, patent document CN118716463a discloses a composition for promoting the healthy growth of bullfrog, which contains components such as compound Chinese herbal medicines, compound vitamins, compound trace elements and the like, and can provide antibacterial and anti-inflammatory effects, immunity enhancement, digestion promotion and other effects, thereby reducing the prevalence of bullfrog adults and promoting weight gain. Patent document CN119498453A provides a feed for tadpoles of thorns and chest in metamorphosis stage, which utilizes compound vitamins to improve the tolerance of tadpoles in metamorphosis stage to high protein feed, thereby achieving the effects of reducing the death rate of tadpoles, enhancing protein absorption and utilization and promoting metamorphosis. Vitamin a, which is an essential fat-soluble vitamin and a key signal molecule for hormonal activity, is a key substrate for retinol metabolism, and can influence tadpole metamorphosis by regulating thyroid hormone synthesis. However, frog-like vitamin a supplementation has a high degree of species specificity and dose sensitivity. Tazawa et al (development. Growth differ. 2017, 59, 688-700) performed high concentration vitamin a (1-20 IU/mL) soaking treatment of the cut rana japonica tadpole at a much higher level than physiological level, resulting in ectopic tail tissue to form hindlimbs, indicating that too high doses of vitamin a may completely subvert the normal development process. Arkin et al (Zoo Biology, 2024, 43, 169-177) tested the effect of vitamin A (0.033-0.066. Mu.g/mL retinol acetate) added to fish fillet feed on growth and development of tadpoles of the bullfrog, and found that tadpole mortality was higher and that individuals who were successfully metamorphosed were smaller. In the field of farming practice, although vitamin a is commonly added as a premix ingredient, the optimal dosage range for promoting growth and driving allergies has been lacking in accurate experimental data support. Insufficient vitamin a addition to the feed may lead to stunted growth and metamorphosis, while excessive addition may lead to wastage, potential toxicity and even malformation. Conventional frog feeds lack vitamin a supplementation strategies formulated for bullfrog. In order to solve the above problems, there is a need to develop a novel bullfrog feed composition containing vitamin a. Disclosure of Invention The application aims to provide a bullfrog feed composition which has a finely regulated vitamin A level, can promote healthy and rapid growth of bullfrog tadpoles and remarkably accelerate metamorphosis development of the bullfrog tadpoles. In order to achieve the above purpose, the present application provides the following technical solutions: a bullfrog feed composition comprises the following components: 40-60% of protein source, 30-40% of carbon source, 6-12% of fat source and the balance of additive, wherein in the additive, mineral supplement accounts for 1-7% of total weight of the bullfrog feed composition, vitamin supplement accounts for 0.1-5% of total weight of the bullfrog feed composition, and the vitamin A level of the bullfrog feed composition is 2000-16000 IU/kg. The application also provides the following technical scheme: A bullfrog feed comprises the bullfrog feed composition as dry matter. The application also provides the following technical scheme: the application of the bullfrog feed in bullfrog cultivation. The technical scheme provided by the application has the following beneficial effects: (1) Based on unique nutritional requirements and physiological characteristics of the bullfrog tadpole, the application systematically mixes and synergistically designs proteins, fat, carbohydrates, minerals and vitamins, and provides a high-performance feed compositio