CN-122004362-A - Tryptophan-added bullfrog feed composition and application thereof

CN122004362ACN 122004362 ACN122004362 ACN 122004362ACN-122004362-A

Abstract

The application provides a bullfrog feed composition added with tryptophan and application thereof. The bullfrog feed composition comprises 40-60% of protein source, 20-30% of carbon source, 6-15% of fat source and the balance of additive, wherein in the additive, mineral supplement accounts for 1-7% of total weight of the bullfrog feed composition, vitamin supplement accounts for 0.1-5% of total weight of the bullfrog feed composition, amino acid supplement accounts for 1-15% of total weight of the bullfrog feed composition, and tryptophan level of the bullfrog feed composition is 0.20-0.60%. The bullfrog feed composition is used for bullfrog cultivation, can accelerate growth and metamorphosis development of bullfrog tadpoles under the premise of guaranteeing basic nutrition supply and health maintenance, shortens cultivation period, and improves seedling emergence uniformity and yield, thereby improving overall production efficiency and economic benefit, and has definite practical value and wide popularization prospect.

Inventors

ZHANG CHUNXIAO
WANG LING
LU KANGLE
LI XUESHAN
SONG KAI
MA RUIJUAN
GAO JUAN

Assignees

集美大学

Dates

Publication Date: 20260512
Application Date: 20260309

Claims (10)

1. The bullfrog feed composition is characterized by comprising the following components in percentage by weight: 40-60% of protein source, 20-30% of carbon source, 6-15% of fat source and the balance of additive, wherein in the additive, mineral supplement accounts for 1-7% of total weight of the bullfrog feed composition, vitamin supplement accounts for 0.1-5% of total weight of the bullfrog feed composition, amino acid supplement accounts for 1-15% of total weight of the bullfrog feed composition, and tryptophan level of the bullfrog feed composition is 0.20-0.60%.
2. The bullfrog feed composition according to claim 1, wherein the protein source is selected from any one of animal protein material, vegetable protein material, and combinations thereof; Preferably, the protein source components in the bullfrog feed composition comprise fish meal, corn gluten meal, gelatin and fish dissolving pulp, wherein the fish meal is any one of Engraulis japonicus Temminck et Schlegel powder, chai Yufen, ba Sha Yufen, sardine powder, tuna powder and a combination thereof, the content of the fish meal in the bullfrog feed composition is preferably 2-15%, more preferably 7%, the content of the corn gluten meal in the bullfrog feed composition is preferably 5-40%, more preferably 35%, the content of the gelatin in the bullfrog feed composition is preferably 0-10%, more preferably 5%, and the content of the fish dissolving pulp in the bullfrog feed composition is preferably 2-5%, more preferably 3%.
3. The bullfrog feed composition according to claim 1, wherein the carbon water source is selected from any one of corn flour, wheat bran, brown rice flour, microcrystalline cellulose, and combinations thereof; Preferably, the carbon water source component in the bullfrog feed composition comprises high-gluten flour and microcrystalline cellulose, wherein the content of the high-gluten flour in the bullfrog feed composition is preferably 20-25%, more preferably 22%, and the content of the microcrystalline cellulose in the bullfrog feed composition is preferably 0-5%, more preferably 2.43%.
4. The bullfrog feed composition according to claim 1, wherein the fat source is selected from any one of vegetable oil, animal fat, lecithin, and combinations thereof; Preferably, the fat source components in the bullfrog feed composition comprise soybean oil and lecithin, wherein the content of the soybean oil in the bullfrog feed composition is preferably 1-10%, more preferably 4.3%, and the content of the lecithin in the bullfrog feed composition is preferably 1-10%, more preferably 4.3%.
5. The bullfrog feed composition according to claim 1, wherein the mineral supplement contains an inorganic salt providing mineral elements, optionally together with a carrier, preferably the inorganic salt is selected from any one of calcium salt, phosphorus salt, potassium salt, sodium salt, magnesium salt, iron salt, zinc salt, copper salt, manganese salt, cobalt salt, iodine salt, selenium salt and combinations thereof; preferably, the mineral supplement composition in the bullfrog feed composition comprises a multi-mineral premix, monocalcium phosphate and calcium carbonate; wherein the multi-mineral premix comprises potassium chloride, potassium iodide, cobalt sulfate, copper sulfate, ferrous sulfate monohydrate, zinc sulfate monohydrate, manganese sulfate monohydrate, magnesium sulfate heptahydrate, sodium selenite and zeolite powder; the content of the multi-mineral premix in the bullfrog feed composition is preferably 0.1-1%, more preferably 0.5%, the content of the monocalcium phosphate in the bullfrog feed composition is preferably 1-5%, more preferably 4%, the content of the calcium carbonate in the bullfrog feed composition is preferably 1-5%, more preferably 1.8%, and the multi-mineral premix in the bullfrog feed composition preferably comprises, by weight, 100-300 parts of potassium chloride, 20-100 parts of potassium iodide, 50-150 parts of cobalt sulfate, 10-40 parts of copper sulfate, 300-500 parts of ferrous sulfate monohydrate, 100-300 parts of zinc sulfate monohydrate, 50-100 parts of manganese sulfate monohydrate, 500-1000 parts of magnesium sulfate heptahydrate, 20-80 parts of sodium selenite and 2000-4000 parts of zeolite powder.
6. The bullfrog feed composition according to claim 1, characterized in that the vitamin supplement contains vitamins selected from any one of vitamin a, vitamin D, vitamin E, vitamin K, vitamins C, B vitamins and combinations thereof, optionally together with antioxidants and/or carriers, the carrier in the vitamin supplement preferably being flour, the antioxidants in the vitamin supplement preferably being ethoxyquinoline; Preferably, the vitamin supplement composition in the bullfrog feed composition comprises a multi-vitamin premix, choline chloride and vitamin C; wherein the multi-vitamin premix comprises vitamin B1, riboflavin, pyridoxine hydrochloride, vitamin B12, vitamin K3, inositol, calcium pantothenate, nicotinic acid, folic acid, biotin, vitamin A, vitamin D3, vitamin E, ethoxyquinoline, and flour; the vitamin A is selected from any one of retinol, retinol propionate, retinol acetate, retinol palmitate and combinations thereof, preferably retinol acetate, the content of the multidimensional premix in the bullfrog feed composition is preferably 0.1-1%, more preferably 0.28%, the content of the choline chloride in the bullfrog feed composition is preferably 0.1-1%, more preferably 0.5%, the content of the vitamin C in the bullfrog feed composition is preferably 0.1-1%, more preferably 0.1%, preferably the multidimensional premix in the bullfrog feed composition comprises 5-15 parts by weight of vitamin B1, 5-15 parts by weight of riboflavin, 5-15 parts by weight of pyridoxine hydrochloride, 0.1-0.5 parts by weight of vitamin B12, 5-15 parts by weight of vitamin K3, 50-150 parts by weight of inositol, 10-30 parts by weight of calcium pantothenate, 30-80 parts by weight of nicotinic acid, 1-5 parts by weight of vitamin A, 8000 parts by weight of vitamin B, 10-200 parts by weight of vitamin E, 200-200 parts by weight of vitamin E, and 120 parts by weight of vitamin E.
7. The bullfrog feed composition according to claim 1, wherein the amino acid supplement contains amino acids selected from any one of tryptophan, alanine, lysine, arginine, histidine, isoleucine, leucine, methionine, phenylalanine, threonine, valine, cystine, tyrosine and combinations thereof, optionally together with antioxidants and/or carriers; Preferably, the amino acid supplement composition comprises amino acid premix, alanine and L-tryptophan, wherein the amino acid premix comprises valine, isoleucine, threonine, lysine, histidine, arginine and tyrosine, the content of the amino acid premix in the bullfrog feed composition is preferably 1-15%, more preferably 9.39%, the content of the alanine in the bullfrog feed composition is preferably 0-1%, more preferably 0-0.3%, the content of the L-tryptophan in the bullfrog feed composition is preferably 0-1%, more preferably 0.1-0.4%, and the amino acid premix in the bullfrog feed composition comprises valine, isoleucine, threonine, lysine hydrochloride, histidine hydrochloride, arginine hydrochloride and tyrosine in amounts of 0.5-1 parts to 1 parts, 0.5-3 parts, and 0.5-3 parts.
8. Bullfrog feed composition according to claim 1, characterized in that the tryptophan level of the bullfrog feed composition is 0.29-0.50%, more preferably 0.36-0.43%; optionally, the bullfrog feed composition has a protein level of 38-50%; Optionally, the bullfrog feed composition has a fat level of 7-12%.
9. A bullfrog feed characterized in that the bullfrog feed composition according to claim 1 is used as a dry matter, and the bullfrog feed is preferably obtained by the following preparation method: a) The bullfrog feed composition of claim 1 wherein the raw materials are weighed and all but the fat source raw materials are mixed; b) Adding water into the mixture obtained in the step a) to carry out tempering; c) Puffing and granulating the quenched and tempered material; d) Drying the puffed granules; e) And applying a fat source raw material to the surface of the particles to obtain the bullfrog feed.
10. Use of the bullfrog feed of claim 9 in bullfrog farming, wherein the bullfrog feed is for feeding bullfrog tadpoles; preferably, the daily feeding amount of the bullfrog feed is 5-8%, preferably 6.5% of the weight of the tadpoles.

Description

Tryptophan-added bullfrog feed composition and application thereof Technical Field The invention belongs to the field of animal nutrition and feed science, in particular to a bullfrog feed composition and application thereof, and particularly relates to a bullfrog feed composition with a fine-adjustment tryptophan level and a method for promoting growth and metamorphosis development of bullfrog tadpoles. Background Bullfrog is an important economic breeding frog, and the breeding period of the bullfrog directly influences the economic benefit. In the bullfrog cultivation period, the synchronicity and uniformity of the tadpole stage growth speed and metamorphosis development are key to determining the whole cultivation efficiency. Tadpole metamorphosis is delayed, and cycle elongation can increase culture cost and risk, and can cause uneven specifications of adult frogs, thereby influencing market income. At present, the practice of fine control of tryptophan levels in feeds and application of the fine control to frog farming is fresh in the industry. Existing conventional feeds are usually supplemented with tryptophan indirectly by increasing dietary protein levels. For example, document CN105851679a adds silkworm chrysalis meal to the feed of the rana spinosa tadpole, and the raw material contains higher levels of methionine, lysine, tryptophan, unsaturated fatty acids, antibacterial peptides and various trace elements, and can partially replace corn gluten meal and fish meal to promote the growth of the rana spinosa tadpole and improve the immune function of the rana spinosa tadpole. The use of a reasonably proportioned amino acid composition to replace part of dietary protein is becoming an important direction for feed nutrition formulation. Mansano et al measured the demand (2.71% on a dry weight basis) for digestible lysine by bullfrog adults (post-metamorphosis stage) and estimated the demand levels (arginine 2.16%, histidine 0.94%, isoleucine 1.34%, leucine 2.39%, methionine 0.79%, phenylalanine 1.31%, threonine 1.34%, tryptophan 0.23%, valine 1.58%, cystine 0.36%, tyrosine 1.07%) for other digestible amino acids based on the ideal essential amino acid ratio, and used to guide the preparation of daily ration containing the appropriate amino acid levels to improve the growth efficiency and protein utilization of bullfrog. Notably, there is a significant species difference in tryptophan metabolism. Unlike most species such as mammals, plasma of aquatic animals such as frogs contains almost no albumin, which results in tryptophan being more easily dissociated in the body and thus more susceptible to excessive tryptophan (int. J. Trytophan. Res. 2022, 15, 11786469221122511). This also means that too high tryptophan content in the feed may trigger toxic reactions. Therefore, the accurate regulation and control of tryptophan level in the frog feed is particularly important for the design of feed nutrition formula. However, there is still a lack of optimization and regulation strategies for tryptophan levels in bullfrog tadpole feeds. In order to solve the above problems, there is a need to develop a novel bullfrog feed composition containing tryptophan. Disclosure of Invention The application aims to provide a bullfrog feed composition which has a finely regulated tryptophan level, can promote healthy and rapid growth of bullfrog tadpoles and remarkably accelerate metamorphosis development of the bullfrog tadpoles. In order to achieve the above purpose, the present application provides the following technical solutions: a bullfrog feed composition comprises the following components: 40-60% of protein source, 20-30% of carbon source, 6-15% of fat source and the balance of additive, wherein in the additive, mineral supplement accounts for 1-7% of total weight of the bullfrog feed composition, vitamin supplement accounts for 0.1-5% of total weight of the bullfrog feed composition, amino acid supplement accounts for 1-15% of total weight of the bullfrog feed composition, and tryptophan level of the bullfrog feed composition is 0.20-0.60%. The application also provides the following technical scheme: A bullfrog feed comprises the bullfrog feed composition as dry matter. The application also provides the following technical scheme: the application of the bullfrog feed in bullfrog cultivation. The technical scheme provided by the application has the following beneficial effects: (1) Based on unique nutritional requirements and physiological characteristics of bullfrog tadpoles, the application systematically mixes and synergistically designs proteins, fat, carbohydrates, minerals, vitamins and amino acids, and provides a high-performance feed composition which has extremely high safety and nutrition utilization efficiency for the bullfrog tadpoles, can fully meet the metabolic requirements of the bullfrog tadpoles, and promote the rapid and healthy growth of the bullfrog tadpoles. (2) The application utilizes the