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CN-122004371-A - Fish feed composite additive based on L-dopamine and preparation method and application thereof

CN122004371ACN 122004371 ACN122004371 ACN 122004371ACN-122004371-A

Abstract

The invention provides a fish feed composite additive based on L-dopamine, a preparation method and application thereof, and relates to the technical field of aquaculture, wherein the fish feed composite additive comprises 0.01-0.05 part of L-dopamine, 45-70 parts of broad bean extract, 10-25 parts of amino acid compound, 2-5 parts of bile acid, 10-20 parts of L-carnitine, 5-10 parts of Chinese herbal medicine composition and 3-8 parts of composite emulsifier. Multiple synergistic effects are generated with components such as broad bean extract, amino acid complex, bile acid, chinese herbal medicine composition, compound emulsifier and the like through the neuroendocrine regulation and control effect of L-dopamine, so that multichannel accurate regulation and control of tilapia muscle structure is realized.

Inventors

  • HUANG JIAMING
  • YANG XINGLI
  • LUO WEITAO
  • CHEN WEIJUN
  • LI HAOXUAN
  • HAN QUAN
  • Ye Canxun
  • JIANG YONGJIE
  • CHEN LU
  • LIN ZHIYING

Assignees

  • 恒兴智慧农业发展(广州)有限公司

Dates

Publication Date
20260512
Application Date
20260318

Claims (10)

  1. 1. The fish feed composite additive based on the L-dopamine is characterized by comprising, by mass, 0.01-0.05 part of L-dopamine, 45-70 parts of broad bean extract, 10-25 parts of amino acid compound, 2-5 parts of bile acid, 10-20 parts of L-carnitine, 5-10 parts of Chinese herbal medicine composition and 3-8 parts of composite emulsifier.
  2. 2. The fish feed composite additive based on L-dopamine according to claim 1, wherein the composite emulsifier comprises a modified phospholipid and a bile acid salt in a mass ratio of (2-4): 1.
  3. 3. The L-dopamine-based fish feed composite additive according to claim 2, wherein the modified phospholipid is pulsed electric field treated soybean lecithin; and/or, the bile acid salt comprises glycocholate and taurocholate with the mass ratio of 2-3:1.
  4. 4. The fish feed composite additive based on L-dopamine according to claim 3, wherein the preparation method of the modified phospholipid comprises the following steps: Dissolving soybean lecithin in water to obtain soybean lecithin solution, preheating the soybean lecithin solution, and then placing the soybean lecithin solution in a pulse electric field for treatment to obtain modified phospholipid; wherein the mass percentage concentration of the soybean lecithin solution is 2-5%; the preheating temperature is 70-80 ℃; And/or the conditions of the pulsed electric field comprise pulse intensity of 10-30 kV/cm, pulse treatment time of 100-500 mu s, and pulse frequency of 300-350 Hz; And/or, the glycocholate is glycocholate sodium salt; And/or, the taurocholate is sodium taurocholate.
  5. 5. The L-dopamine-based fish feed composite additive according to claim 1, wherein the amino acid composite comprises lysine, methionine and tryptophan in a mass ratio of (4-6): 2-4): 1-3; And/or the Chinese herbal medicine composition comprises astragalus membranaceus, eucommia ulmoides, sea buckthorn and honeysuckle in the mass ratio of (2-4): 1-3.
  6. 6. A method for preparing the L-dopamine-based fish feed composite additive according to any one of claims 1 to 5, comprising the steps of: s1, crushing and uniformly mixing broad bean extract, amino acid compound, L-carnitine and Chinese herbal medicine composition to obtain mixed powder; S2, dissolving L-dopamine in water to obtain an L-dopamine solution, and then adding bile acid and a composite emulsifier to mix to obtain a pre-emulsion; and S3, placing the mixed powder into a mixer for mixing, spraying the pre-emulsion onto the flowing powder, continuously mixing, and drying to obtain the composite additive.
  7. 7. The method for preparing a fish feed composite additive based on L-dopamine according to claim 6, wherein in the step S1, the average particle size of the mixed powder is less than or equal to 50 μm; And/or, in step S2, dissolving L-dopamine in water at 35-40 ℃; and/or the mass percentage concentration of the L-dopamine solution is 0.3-0.5%; And/or in the step S2, the mixing is performed by adopting a high-speed shearing emulsifying machine, and shearing and stirring for 3-5min at the rotating speed of 4000-5000 rpm.
  8. 8. The method for preparing the compound additive for fish feed based on L-dopamine according to claim 6, wherein in the step S3, the rotating speed of the mixer is 15-25 rpm, the spraying time of the pre-emulsion is 10-15min, then the spray gun is closed, the mixing is continued for 15-20min, and the total mixing time is not less than 25min; and/or in the step S3, the drying is carried out for 12-24 hours under the vacuum degree of 40-45 ℃ and minus 0.08 to minus 0.05 MPa.
  9. 9. Use of the L-dopamine-based fish feed composite additive according to any one of claims 1 to 5 or the L-dopamine-based fish feed composite additive prepared by the preparation method of the L-dopamine-based fish feed composite additive according to any one of claims 6 to 8, characterized in that the L-dopamine-based fish feed composite additive is used in the preparation of fish feed.
  10. 10. The use according to claim 9, characterized in that the L-dopamine-based fish feed composite additive is added in an amount of 1-5% of the total mass of the fish feed.

Description

Fish feed composite additive based on L-dopamine and preparation method and application thereof Technical Field The invention relates to the technical field of aquaculture, in particular to a fish feed composite additive based on L-dopamine and a preparation method and application thereof. Background Tilapia is a worldwide important aquaculture species, and is favored because of its fast growth and strong adaptability. However, the common tilapia meat is soft, has poor taste and limited market value. In order to improve the quality of tilapia meat, cultivation of tilapia with crisp meat becomes a research hot spot. The crisp tilapia is tilapia with more compact muscle tissues, high elasticity and chewiness by a special feeding process, and the market value of the crisp tilapia is 30-50% higher than that of common tilapia. At present, the embrittlement culture of tilapia mainly adopts a method of feeding broad beans. The broad beans contain special components, so that the muscle tissue structure of the fish body can be changed, and the muscle hardness and the chewiness can be improved. As disclosed in patent CN113729128B, a feed additive for promoting embrittlement of tilapia meat is disclosed, which comprises a broad bean aqueous extract and an amino acid compound as main components, and can increase the growth rate of tilapia compared with pure feeding of broad beans. However, the method has the obvious defects that the embrittlement period is long (more than 60 days are usually required), the meat quality is not uniform, and the growth inhibition of tilapia and the burden on the liver are increased due to the long-term feeding of broad beans. Another prior art CN107981103A introduces a functional additive for tilapia, which comprises Chinese herbal medicine components such as astragalus, eucommia bark, sea buckthorn, honeysuckle and the like, improves the disease resistance and survival rate of the tilapia, and improves the meat quality and flavor. However, the direct effect of this additive on muscle embrittlement is limited and does not involve neuroendocrine regulatory mechanisms. Research shows that the formation and regulation of fish muscle texture involve complex physiological and biochemical processes including muscle fiber type conversion, collagen deposition, energy metabolism regulation and the like. The traditional embrittlement method mainly depends on unknown components in broad beans, has single action and lacks systematic regulation. In addition, the prior art pays attention to insufficient stress reaction and health maintenance of fish in the embrittlement process, and the death rate in the embrittlement process is often increased. Therefore, a novel technical scheme capable of rapidly, efficiently and safely improving the quality of crisp tilapia meat without affecting or even promoting the growth and health of the tilapia meat is urgently needed in the market. Disclosure of Invention The invention aims to provide an L-dopamine-based fish feed composite additive, a preparation method and application thereof, wherein L-dopamine is introduced into the field of aquatic feed for the first time, and multiple synergistic effects are generated with components such as broad bean extract, amino acid compound, bile acid, chinese herbal medicine composition, compound emulsifier and the like through the neuroendocrine regulation and control effect of the L-dopamine, so that the multichannel accurate regulation and control of tilapia muscle structure is realized. In order to achieve the above purpose, the present invention adopts the following technical scheme: The invention provides an L-dopamine-based fish feed composite additive which comprises, by mass, 0.01-0.05 part of L-dopamine, 45-70 parts of broad bean extract, 10-25 parts of amino acid composite, 2-5 parts of bile acid, 10-20 parts of L-carnitine, 5-10 parts of Chinese herbal medicine composition and 3-8 parts of composite emulsifier. Furthermore, on the basis of the technical scheme, the composite emulsifier comprises modified phospholipid and bile acid salt with the mass ratio of (2-4) to 1. Furthermore, on the basis of the technical scheme, the modified phospholipid is soybean lecithin treated by a pulse electric field; and/or, the bile acid salt comprises glycocholate and taurocholate with the mass ratio of 2-3:1. Furthermore, on the basis of the technical scheme, the preparation method of the modified phospholipid comprises the following steps: Dissolving soybean lecithin in water to obtain soybean lecithin solution, preheating the soybean lecithin solution, and then placing the soybean lecithin solution in a pulse electric field for treatment to obtain modified phospholipid; wherein the mass percentage concentration of the soybean lecithin solution is 2-5%; the preheating temperature is 70-80 ℃; And/or the conditions of the pulsed electric field comprise pulse intensity of 10-30 kV/cm, pulse treatment time of 100-500 mu s, and pulse