CN-122004373-A - Semi-artificial feed for porous cross-ditch elephant and artificial feeding method thereof

Abstract

The invention belongs to the technical field of insect feeding, and particularly relates to a semi-artificial feed for a porous transverse ditch image and an artificial feeding method thereof. The semi-artificial feed for the porous cross ditch elephant provided by the invention is prepared from ash bark powder of ash tree, ash tree wood side material powder, soybean powder, sucrose, agar, cholesterol, welch salt, potassium sorbate, methyl p-hydroxybenzoate, vitamin B complex and distilled water according to a specific weight portion ratio. The artificial feeding method comprises the whole procedures of adult collection, spawning induction, egg hatching, single-head larva feeding, pupa management and adult mixed culture. The feed disclosed by the invention is comprehensive and balanced in nutrition, can fully meet the whole life history requirements of the porous rhabdoelephant, remarkably improves the survival rate of larvae and the emergence rate of adults, realizes large-scale standardized feeding, and provides a stable insect source for pest control research.

Inventors

• JI YINGCHAO
• ZHENG XUE
• CHEN WEILI
• SONG CHENYU
• Gao Quanbin
• WANG YUHAN
• LIU YANXUE

Assignees

• 山东农业大学

Dates

Publication Date

20260512

Application Date

20260311

Claims (9)

1. 1. The semi-artificial feed for the porous transverse ditch elephant is characterized by comprising, by weight, 10-15 parts of ash bark powder, 10-15 parts of ash tree xylem edge powder, 9-11 parts of soybean powder, 3-5 parts of sucrose, 5-7 parts of agar, 0.05-0.1 part of cholesterol, 0.2-0.4 part of Welch salt, 0.2-0.4 part of methyl sorbate, 0.2-0.4 part of methyl p-hydroxybenzoate, 0.5-1.5 parts of vitamin B complex and 100-200 parts of distilled water.
2. 2. The semi-artificial feed according to claim 1, wherein the fineness of the ash phloem powder and ash xylem side material powder is 70-90 mesh, and the fineness of potato powder is 90-110 mesh.
3. 3. The method for preparing the semi-artificial feed according to claim 1, comprising the following steps: s1, uniformly mixing ash tree phloem powder, ash tree xylem side material powder and soybean powder, and cooling to room temperature after steam sterilization to obtain a sterilized material; s2, adding water into sucrose, cholesterol, welch salt, potassium sorbate, methyl p-hydroxybenzoate and vitamin B complex, stirring and dissolving to obtain a mixed material, adding agar into boiling water until the agar is melted, and forming an agar solution; s3, adding the sterilized material and the mixed material into the agar solution, uniformly mixing, naturally cooling to room temperature, and solidifying to obtain the semi-artificial feed.
4. 4. The method according to claim 3, wherein the sterilization condition is 110 ℃ to 130 ℃ for 20 to 30 minutes.
5. 5. An artificial breeding method of the hemimorphic elephant, which is characterized by comprising the step of breeding the hemimorphic elephant larva on the semi-artificial feed according to any one of claims 1 to 2, wherein the method is used for shortening the larva development period and improving the larva survival rate and pupation rate.
6. 6. The artificial feeding method according to claim 5, wherein the step of obtaining the multi-well transverse ditch larvae is as follows: (1) Collecting the adult multi-hole cross-ditch, placing the adult multi-hole cross-ditch in a feeding box, wherein the feeding density is 1-2 heads/cm < 2 >, placing fresh fraxinus chinensis branches in the feeding box, replacing 1-2 times a day, and collecting egg grains in the feeding box every 20-30 hours; (2) Eggs are placed in a constant temperature incubator with the temperature of 26-28 ℃ and the relative humidity of 85-95% for hatching, and the hatched larvae are collected.
7. 7. The artificial feeding method according to claim 6, wherein the adult feeding condition is 20 ℃ to 30 ℃ and the relative humidity is 70% to 80%.
8. 8. The artificial feeding method according to claim 6, wherein the feeding condition of the larvae is that the hatched larvae are fed into a feeding box with the semi-artificial feed, and the larvae are fed under the constant temperature condition of 26-28 ℃, relative humidity of 60-70% and light-dark period of 12h/12h, and fresh feed is replaced for 1-2 times every 9-11 days until pupation.
9. 9. The artificial feeding method according to claim 8, wherein after the pupae emerge as adults, the pupae are mixedly fed into a feeding box according to a ratio of 1:1, fresh fraxinus chinensis branches are placed in the feeding box, the fraxinus chinensis branches are replaced 1-2 times a day, eggs in the feeding box are collected every 20-30 hours, and the feeding is repeated until the pupae emerge.

Description

Semi-artificial feed for porous cross-ditch elephant and artificial feeding method thereof Technical Field The invention belongs to the technical field of insect feeding, and particularly relates to a semi-artificial feed for a porous transverse ditch image and an artificial feeding method thereof. Background The porous transverse ditch image Pimelocerusperforatus, the great name of the transverse ditch image DysceruscribripennisMatsumura et K ō no. The porous transverse ditch is a destructive boring pest, has wide host range and mainly damages various important economic woods and ornamental plants such as olive, white wax, light wax tree, japanese glossy privet, syringa amurensis, osmanthus fragrans and the like. The pest damage mode is very destructive, female adults lay eggs on the trunk base, and after larvae hatch, the trunk base is eaten by drilling to damage, so that phloem of the trunk base of a damaged plant falls off to form a damage of 'thousand sore hundred holes', and finally the tree vigor is weakened or even dead. The life history of the porous transverse ditch image is extremely complex, and obvious generation overlapping phenomenon exists. The overwintering insect mode is various, not only the adults of different years can overwinter in soil layers under the trunk basal part peripheral dead leaf weeds, but also the larvae of different ages hatched in the same year can overwinter in phloem or xylem moth of the trunk basal part and the root neck part. Overwintering adults first come out of the jellyfish in the late 3 months, gather for a period of time on a dry basis, then climb onto branches and leaves of a crown to gnaw branches and barks to supplement nutrition, then climb back to the dry basis to mate and spawn, and the actions of supplementing nutrition on the upper tree and mating and spawning on the lower tree are performed reciprocally. The peak period of the adult out-of-sting is the middle and late 4 months and the end of the out-of-sting is the late 5 months. In the early period of hazard, adults gather at the base of the white wax trunk, rest and climb to the crown to take food to supplement nutrition, and then go down the tree after supplementing nutrition, and lay eggs at the root and neck at the base of the white wax trunk or under the ground. The low-age larvae are damaged at phloem under old and coarse skin, brown worm manure and scraps are discharged at the boreholes or skin seams to be in a rust brown moist shape, and the medium-age larvae drill wood side edge parts, so that the manure scraps are not discharged outwards any more, but fill in the boreholes and wrap the larvae. Mature larvae construct pupae chambers around the larvae with dry wood wool cuttings in the basal half of the moth way. For trees with larger breast diameters, larvae can be damaged at the positions with the height of 1.5-2.0 m, yellow viscous liquid with peculiar smell flows out of the positions of the cavities, and flows down along the trunks. Once the young worms eat the tree trunk for one week, the tree dies. Currently, research on multi-aperture transverse ditch images is mainly focused on hazard characteristic investigation. The biological habit and the ecological characteristic of the pests can be deeply known through the artificial feeding technology, so that important support is provided for the research and development of the comprehensive control technology of the pests, and standardized experimental materials can be provided for related biological assay experiments. Therefore, there is a need to establish a technical method capable of stably feeding each insect state and obtaining a large number of standard test insects with consistent development so as to meet the demands of comprehensive prevention and control research, scientific behavioral research and the like of the porous transverse ditch. In the prior art, the breeding of the porous transverse ditch image is mostly dependent on the rhizome parts or branches of natural host plants, but the breeding mode has a plurality of defects that on one hand, the acquisition of natural host materials is limited by seasons and regions and the annual continuous breeding cannot be realized, and on the other hand, the natural hosts are unstable in nutrition ingredients, so that the test insects are inconsistent in development, the survival rate and the emergence rate are lower, and the large-scale and standardized experiment requirements are difficult to meet. In addition, the partially tried artificial feed formula has the problems of unbalanced nutrition, difficult acquisition of raw materials, complex preparation or overhigh cost and the like, and cannot realize the efficient feeding of the porous transverse ditch elephant in the whole life history. Disclosure of Invention Based on the technical problems, the invention aims to provide a semi-artificial feed for a porous transverse ditch elephant and an artificial feeding method thereof. In order