CN-122004456-A - Morchella fermentation-based pollen pini-lotus leaf composition, and preparation method and application thereof

Abstract

The invention belongs to the technical field of food processing and biological fermentation, and particularly relates to a pine pollen-lotus leaf composition based on Morchella fermentation, a preparation method and application thereof. According to the invention, pine pollen and lotus leaves are fermented by Morchella, and the obtained composition has remarkable effects of losing weight and reducing blood fat.

Inventors

• QIAO QIANG
• ZENG MAOMAO
• YIN LEI
• CHEN JIE
• WANG TONG
• HE ZHIYONG
• XU HONGNAN
• Ai Baila.reheman

Assignees

• 烟台新时代健康产业有限公司

Dates

Publication Date

20260512

Application Date

20260211

Claims (8)

1. 1. The pine pollen-lotus leaf composition based on Morchella fermentation is characterized by being prepared by fermenting a compound substrate of pine pollen and lotus leaf, wherein the weight ratio of the pine pollen to the lotus leaf is 3-6:1.
2. 2. A method of preparing a morchella fermentation-based pollen Pini-lotus leaf composition as claimed in claim 1, comprising the steps of: S1, mixing wall-broken pine pollen with lotus leaf powder to obtain a compound raw material; s2, adding deionized water into the compound raw materials, adding glucose, sterilizing, and cooling; s3, inoculating Morchella spore suspension into the cooled system, wherein the inoculum size is 5% -15%, and fermenting to obtain fermentation liquor; S4, inactivating the fermentation liquor, centrifuging to obtain supernatant, concentrating the supernatant, and drying to obtain the Morchella fermentation-based pollen Pini-lotus leaf composition.
3. 3. The preparation method of the morchella fermentation-based pollen pini-lotus leaf composition according to claim 2, wherein in the step S2, the feed liquid ratio of the compound raw material to deionized water is 1:10-1:25, and the final concentration of glucose is 15 g/L-25 g/L.
4. 4. A method of preparing a morchella fermentation-based pollen Pini-lotus leaf composition as claimed in claim 2, wherein in step S2, the sterilization treatment is pasteurization at 80-90 ℃ for 20-40 minutes.
5. 5. A method for preparing a morchella fermentation-based pollen Pini-lotus leaf composition as claimed in claim 2, wherein in step S2, the sterilization treatment is high pressure sterilization at 105-121 ℃ for 5-20 minutes.
6. 6. The preparation method of the Morchella fermentation-based pollen Pini-lotus leaf composition as claimed in claim 2, wherein in the step S3, the fermentation condition is that the temperature is 26+ -2deg.C, the stirring speed is 120-200 rpm, the time is 3-7 days, and the Morchella spore suspension concentration is 1×10 6 ～5×10 6 spores/mL.
7. 7. The use of the morchella fermentation-based pollen Pini-lotus leaf composition as claimed in claim 1 in preparing functional food or health food with weight reducing or blood lipid lowering effects.
8. 8. The method according to claim 7, wherein the functional food or health food is in the form of a solid beverage, a tableted candy or a capsule.

Description

Morchella fermentation-based pollen pini-lotus leaf composition, and preparation method and application thereof Technical Field The invention relates to a pine pollen-lotus leaf composition based on Morchella fermentation, a preparation method and application thereof, and belongs to the technical field of food processing and biological fermentation. Background Obesity has become a global public health problem. The normal energy balance caused by high fat diet causes abnormal accumulation of white fat and is often accompanied by a series of metabolic problems such as blood lipid disorder, liver steatosis and low-grade inflammation. In the field of natural product development, pollen pini and lotus leaf are two interesting medicinal and edible raw materials. Pollen Pini is rich in sterol, polysaccharide and flavone, and has effects of reducing serum Total Cholesterol (TC) and Triglyceride (TG) as shown by researches. The lotus leaf is a dry leaf of lotus belonging to the Nymphaeaceae family, contains nuciferine, flavone, polysaccharide and the like, and researches show that the active ingredients of the lotus leaf have the functions of reducing blood fat, resisting oxidization and inhibiting fatty acid synthase, and can promote fatty acid beta-oxidization and reduce liver fat deposition. However, in the traditional application mode, pollen Pini and lotus leaf are often eaten by direct brewing or crushing, and the simple physical treatment mode is difficult to effectively release and transform the active ingredients in the pollen Pini and the lotus leaf, so that the further application and efficacy exertion of the pollen Pini and the lotus leaf are limited. Microbial fermentation technology is considered as a potential means for improving flavor and efficacy of the edible and pharmaceutical raw materials. The method can enhance the bioactivity of the raw materials by destroying plant cell walls, converting glycoside components, and increasing total phenol and flavone content. However, at present, how to apply the microbial fermentation technology to the composition of pollen Pini and folium Nelumbinis in a targeted manner has not been reported in the mature technical scheme. Disclosure of Invention Aiming at the technical problems in the prior art, the invention provides a pine pollen-lotus leaf composition based on Morchella fermentation, and a preparation method and application thereof. The technical scheme for solving the technical problems is as follows: The invention aims to provide a Morchella fermentation-based pollen Pini-lotus leaf composition, which is prepared by fermenting a compound substrate of pollen Pini and lotus leaf with Morchella, wherein the weight ratio of pollen Pini to lotus leaf is 3-6:1. The technical scheme has the beneficial effects that after the pine pollen and the lotus leaf are fermented by Morchella, the cell wall structures of the pine pollen and the lotus leaf can be effectively damaged, the release and conversion of active ingredients such as flavone and the like in the pine pollen and the lotus leaf are promoted, the dissolution rate and bioavailability of the active ingredients are improved, the composition can be better absorbed and utilized after entering a human body, and further the physiological activities such as blood fat reduction and fat metabolism promotion of the composition are enhanced. Further, the composition is dry powder, and the moisture content is less than or equal to 8%. Further, the weight ratio of the pine pollen to the lotus leaf is 5:1. Further, the total phenol content of the composition is more than or equal to 5.5 mg Gallic Acid Equivalent (GAE)/g dry product, and the total flavone content is more than or equal to 4.5 mg Rutin Equivalent (RE)/g dry product. The second aim of the invention is to provide a preparation method of the pine pollen-lotus leaf composition based on Morchella fermentation, which comprises the following steps: S1, mixing wall-broken pine pollen with lotus leaf powder to obtain a compound raw material; s2, adding deionized water into the compound raw materials, adding glucose, sterilizing, and cooling; s3, inoculating Morchella spore suspension into the cooled system, wherein the inoculum size is 5% -15% (v/v), and fermenting to obtain fermentation liquor; S4, inactivating the fermentation liquor, centrifuging to obtain supernatant, concentrating the supernatant, and drying to obtain the Morchella fermentation-based pollen Pini-lotus leaf composition. The preparation method of the invention has the beneficial effects that the efficient fermentation and conversion of the pine pollen and lotus leaf compound substrate are realized, specifically, the wall-broken pine pollen is adopted as the raw material, the utilization and conversion of the Morchella to nutrient components are facilitated, glucose is added as a carbon source to promote the growth and metabolism of the Morchella, the sterilization treatment eliminates the cont