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CN-122004463-A - Ginkgo fruit peptide-containing aqueous extract for improving bone joint health and application thereof

CN122004463ACN 122004463 ACN122004463 ACN 122004463ACN-122004463-A

Abstract

The invention provides a ginkgo fruit peptide-containing aqueous extract for improving bone joint health and application thereof, and the preparation method of the ginkgo fruit peptide-containing aqueous extract is as follows: adding drinking water into cooked ginkgo fruit powder, pulping, adding any two or more enzymes selected from amylase, alkaline protease, neutral protease and cellulase for step-by-step enzymolysis, separating solid from liquid after enzymolysis, concentrating filtrate, and spray drying to obtain ginkgo fruit peptide-containing water extract. The ginkgo fruit peptide-containing aqueous extract prepared by the specific enzymolysis technology has the advantages that the polysaccharide content and the peptide content are obviously increased, and the ginkgo fruit peptide-containing aqueous extract has the function characteristic of improving the health of bone joints. The preparation process provided by the invention has the advantages of stability and mild conditions, can effectively improve the extraction efficiency and the bioavailability of the active ingredients, and provides a high-quality raw material basis for developing natural foods, health-care products or medicines with the functions of protecting articular cartilage, relieving inflammation and relieving osteoarticular related discomfort.

Inventors

  • YANG SONGLIN
  • LI JUNHU
  • YIN RU

Assignees

  • 犀望生物(苏州)有限公司

Dates

Publication Date
20260512
Application Date
20260120

Claims (8)

  1. 1. Application of semen Ginkgo peptide-containing water extract in preparing food, medicine or health product for improving bone joint health is provided.
  2. 2. The use according to claim 1, wherein the preparation method of the ginkgo nut peptide-containing aqueous extract comprises the following steps: S1, aging ginkgo nuts, grinding the ginkgo nuts into powder with fineness grinding value of 60-80 meshes, adding drinking water into the aged ginkgo nut powder according to a feed liquid ratio of 1:8-12, and mixing uniformly to obtain ginkgo nut pulp; s2, adding enzymes into the ginkgo pulp for step-by-step enzymolysis, wherein the enzymolysis temperature is 55-65 ℃, and after the enzymolysis is finished, heating the enzymolysis liquid to 90-100 ℃, and keeping for 0.5-1.0 h to thoroughly inactivate the enzymes; S3, performing solid-liquid separation on the enzymolysis liquid to obtain ginkgo fruit filtrate, concentrating the filtrate to a concentration of 15-30%, and performing spray drying to obtain the ginkgo fruit peptide-containing water extract.
  3. 3. The method according to claim 2, wherein the enzyme used in the step S2 is any two or more of amylase, alkaline protease, neutral protease and cellulase.
  4. 4. The application of the method of claim 3, wherein the addition amount of the amylase is 0.1-2.0 wt% of the mass of the cured ginkgo nut powder, the pH of enzymolysis is 5.0-6.5, and the enzymolysis time is 0.1-1.5 h.
  5. 5. The application of the method of claim 3, wherein the alkaline protease is added in an amount of 0.5-3.0 wt% of the mass of the cured ginkgo nut powder, the pH of the enzymolysis is 7.5-8.5, and the enzymolysis time is 0.5-2.0 h.
  6. 6. The application of the method of claim 3, wherein the addition amount of the neutral protease is 0.5-3.0 wt% of the mass of the cured ginkgo nut powder, the pH of enzymolysis is 7.0-8.0, and the enzymolysis time is 0.2-1.0 h.
  7. 7. The application of the method of claim 3, wherein the addition amount of the cellulase is 0.1-1.0 wt% of the mass of the cured ginkgo nut powder, the pH of enzymolysis is 5.5-6.5, and the enzymolysis time is 0.5-1.0 h.
  8. 8. The method according to claim 1, wherein the food, drug or health product for improving bone joint health comprises a tablet, oral liquid, powder or paste.

Description

Ginkgo fruit peptide-containing aqueous extract for improving bone joint health and application thereof Technical Field The invention belongs to the technical field of food processing, and particularly relates to a ginkgo fruit peptide-containing water extract for improving bone joint health and application thereof. Background Osteoarthritis, bone joint injury and other problems are major health problems that lead to pain, dysfunction and reduced quality of life worldwide, with the pathological core involving progressive wear of articular cartilage, synovial inflammation and degradation of extracellular matrix. At present, clinical treatment mainly comprises nonsteroidal anti-inflammatory drugs and analgesics, and aims at relieving symptoms, but adverse reactions of gastrointestinal tracts, cardiovascular diseases and kidneys are easy to cause after long-term use. Therefore, the development of natural product source functional foods or medicines capable of effectively promoting articular cartilage repair and high in safety has become an important direction in the current life health research and development field. Ginkgo (Ginkgo biloba L.), also known as semen Ginkgo, is a traditional Chinese medicine and medicinal and edible plant, and has effects of astringing lung, relieving asthma, and stopping leukorrhagia and urination. Modern pharmacological research shows that the medicine is rich in active ingredients such as bilobalide, flavonoid, polysaccharide and the like, and has certain potential in the aspects of antioxidation, anti-inflammatory and nervous system protection. However, aiming at the specific field of joint injury repair, the composition and bioavailability of the active ingredients of ginkgo fruit extracts obtained by conventional preparation methods such as traditional water extraction and the like are obviously limited. The researches and applications of ginkgo extracts disclosed in the prior art are concentrated on the respiratory system or the nervous system, and experimental evidence of sufficient and definite articular cartilage protection and regeneration repair functions is generally lacking. This is mainly because the active ingredient may exist in a macromolecular form, it is difficult to penetrate the synovial barrier to reach the cartilage action target, or it does not possess a key active structure (such as a specific small molecule peptide or polysaccharide fragment) that directly regulates chondrocyte metabolism (such as promotion of articular chondrocyte development, inhibition of articular chondrocyte apoptosis and inflammatory response) itself. Therefore, ginkgo nuts are not a typical raw material with a clear joint injury repair function in the prior art. In view of the above, the need for safe and effective restoratives in the field of joint disease treatment is urgent, and the application potential of ginkgo fruit, which is a traditional resource, in the aspect of joint repair is not fully explored due to the limitation of the conventional extraction technology. The prior art fails to provide a preparation method capable of remarkably enhancing and endowing ginkgo nut extract with novel joint injury repair function. This technical gap is the core problem that the present invention aims to solve. The invention provides the novel enzymolysis technology, which aims to break through the bottleneck of the traditional extraction method and activate or release the hidden brand new functional factors in ginkgo fruits, so that the ginkgo fruits are used as efficient functional food or medicine raw materials for joint repair, and the novel ginkgo fruits have obvious technical progress and market application value. Disclosure of Invention Aiming at the defects and the blank existing in the prior art, the invention aims to provide the ginkgo fruit peptide-containing aqueous extract for improving the health of the bone joint and the application thereof, and the novel directional enzymolysis technical means is utilized to accurately control the types, the proportion, the pH value, the enzymolysis temperature and the time of enzymes, selectively hydrolyze the cell walls and macromolecular substances of the ginkgo fruit into small molecule fragments with joint targeting activity, so as to prepare the ginkgo fruit peptide-containing aqueous extract with the function of improving the health of the bone joint, thereby showing the function of improving the health of the bone joint and filling the technical blank of the ginkgo fruit in the field. The technical proposal is that the ginkgo fruit peptide-containing water extract is applied to the preparation of foods, medicines or health care products for improving the health of bone joints. The preparation method of the ginkgo fruit peptide-containing aqueous extract comprises the following steps: S1, aging ginkgo nuts, grinding the ginkgo nuts into powder with fineness grinding value of 60-80 meshes, adding drinking water into the aged ginkgo