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CN-122004474-A - Probiotic microcapsule and preparation method and application thereof

CN122004474ACN 122004474 ACN122004474 ACN 122004474ACN-122004474-A

Abstract

The invention discloses a probiotic microcapsule and a preparation method and application thereof, wherein the probiotic microcapsule has a double protection structure and comprises an inner core and a wall material layer coated outside the inner core; the inner core is composed of porous starch, probiotics are embedded in the inner pore structure of the porous starch, and the wall material layer is composed of cellulose derivatives. Dissolving starch in water to prepare starch solution, dissolving cellulose derivative in water to prepare cellulose solution, mixing the starch solution with the probiotic bacterial suspension, stirring at a speed of 150-200 rpm at 37 ℃ for 0.5-1 hour, then adding the cellulose solution, continuously stirring for 0.5-1 hour, centrifuging the mixed solution at 8000-10000 rpm for 10-30 minutes, collecting precipitate, and freeze-drying to obtain the probiotic microcapsule. The invention constructs a double protection structure through porous starch and cellulose derivatives, realizes high survival rate of probiotics in multiple environments such as processing, digestion, storage and the like, and has the advantage of simple process.

Inventors

  • LI JIADONG
  • YANG QINGYU
  • XU XIANRUI
  • LIU XINNAN

Assignees

  • 中创益科(沈阳)生物技术研究有限公司
  • 中创益科(上海)生物科技有限公司

Dates

Publication Date
20260512
Application Date
20260211

Claims (10)

  1. 1. The probiotic microcapsule is characterized by having a double protection structure and comprising an inner core and a wall material layer coated outside the inner core, wherein the inner core is composed of porous starch, probiotics are embedded in an inner pore structure of the porous starch, and the wall material layer is composed of cellulose derivatives.
  2. 2. The probiotic microcapsule according to claim 1, characterized in that the cellulose derivative is selected from at least one of microcrystalline cellulose, hydroxypropyl methylcellulose.
  3. 3. The probiotic microcapsule according to claim 1, characterized in that the probiotic comprises a combination of one or more of lactobacillus plantarum, lactobacillus acidophilus, lactobacillus rhamnosus, lactobacillus casei, bifidobacterium infantis, bifidobacterium longum, bifidobacterium breve, streptococcus thermophilus and saccharomyces boulardii.
  4. 4. The method for preparing the probiotic microcapsule according to any one of claims 1 to 3, comprising the following steps: S1, dissolving starch in water to prepare a starch solution with the mass concentration of 0.5-2.5%, and dissolving cellulose derivatives in water to prepare a cellulose solution with the mass concentration of 0.1-0.5%; S2, mixing the starch solution obtained in the step S1 with a probiotic bacterial suspension with the concentration of 10 7 -10 9 CFU/ml according to the volume ratio of 2:1 to 1:5, stirring for 0.5 to 1 hour at the temperature of 37 ℃ at the rotating speed of 150 to 200 rpm, then adding the cellulose solution obtained in the step S1, and continuously stirring for 0.5 to 1 hour; S3, centrifuging the mixed solution obtained in the step S2 for 10-30 minutes at 8000-10000 rpm, collecting precipitate, and freeze-drying to obtain the probiotic microcapsule.
  5. 5. The method of claim 4, wherein in step S1, the mass concentration of the starch solution is 1.5% and the mass concentration of the cellulose solution is 0.3%.
  6. 6. The method of claim 4, wherein in step S2, the concentration of the probiotic bacterial suspension is 10 8 CFU/ml, and the mixing volume ratio of the probiotic bacterial suspension to the starch solution is 1:1.
  7. 7. The use of a probiotic microcapsule is characterized in that the probiotic microcapsule is used for preparing a probiotic microcapsule tablet, and the probiotic microcapsule tablet comprises the probiotic microcapsule of claims 1-3 and pharmaceutically or edible auxiliary materials.
  8. 8. The method according to claim 7, wherein the auxiliary materials comprise, by weight, 20-30 parts of maltodextrin, 2-5 parts of microcrystalline cellulose, 15-35 parts of sorbitol, 0.1-0.2 part of citric acid and 0.5-2 parts of magnesium stearate.
  9. 9. The method of claim 8, wherein the probiotic microcapsule tablet is prepared by mixing maltodextrin, microcrystalline cellulose, sorbitol, citric acid and magnesium stearate in proportion for 10-30 minutes, granulating by a wet method, drying, sieving, grinding to obtain mixed auxiliary materials, uniformly mixing the probiotic microcapsule and the mixed auxiliary materials in a proportion of 1:4-2:3 to obtain a tabletting mixture, and pressing 0.5-1.0g of the tabletting mixture under a pressure of 5-15 kPa for 1-10 minutes to obtain the probiotic microcapsule tablet.
  10. 10. Use of a probiotic microcapsule tablet according to any of claims 7-9 for the preparation of a food or pharmaceutical product having a function of regulating intestinal flora.

Description

Probiotic microcapsule and preparation method and application thereof Technical Field The invention relates to the technical field of food and pharmaceutical preparation, in particular to a probiotic microcapsule and a preparation method and application thereof. Background With the rise of health consumption consciousness, the global probiotic product market presents explosive growth. The probiotics have obvious potential in the fields of improving digestion function, enhancing immunity, intervening metabolic diseases and the like due to the regulation effect of the probiotics on intestinal flora. Among them, lactobacillus plantarum (Lactobacillus plantarum) is widely used as a strain with strong acid resistance and excellent colonization ability in fermented foods, dietary supplements and medicines. However, the existing probiotic oral preparation still faces serious challenges that on one hand, the digestive tract environments such as gastric acid, bile salts and the like can greatly reduce the survival rate of living bacteria, and on the other hand, the conventional freeze-dried powder, liquid dosage forms and tablet products are easily affected by temperature and humidity in the storage and transportation processes, so that the bacteria are inactivated. According to research statistics, the loss rate of viable bacteria of commercial probiotics product is generally over 50% in the shelf life, and the function of the probiotic product is seriously restricted. Therefore, development of a probiotic protection technology with both high stability and high survival rate is an urgent need in the industry. In order to improve the tolerance of probiotics in the processing and digestion processes, microcapsule embedding technology is becoming a research hotspot. The technology can effectively block external adverse factors by wrapping thalli in a protective material by a physical or chemical method. The existing microcapsule mostly adopts traditional wall materials such as sodium alginate, chitosan and the like, but has the problems of low embedding rate, insufficient mechanical strength, poor biocompatibility and the like. In contrast, cellulose and its derivatives (e.g., hydroxypropyl methylcellulose) are considered to be ideal novel embedding materials due to their excellent film forming properties, pH responsiveness and degradability. Research shows that the microcapsule taking cellulose as a wall material can maintain structural integrity in gastric juice and release living bacteria in intestinal targeted mode, so that bioavailability is improved. However, the single microcapsule technology still has difficulty in meeting the industrial requirements of the probiotic preparation, and especially in the subsequent tabletting and forming process, the microcapsules are easy to damage the structure due to mechanical pressure or friction heat, so that secondary deactivation is caused. How to realize the cooperative optimization of the microcapsule and tablet process becomes the key of technological breakthrough. In the traditional tabletting process, probiotics are often directly mixed with auxiliary materials such as filler, disintegrating agent and the like and then are compressed, but the mode has obvious damage to the activity of the thalli. The existing research focuses on low-temperature tabletting or adding protective agents, but the problems of high equipment cost, slow tablet disintegration, interference of auxiliary materials on the activity of thalli and the like still exist. Therefore, an integration scheme that combines the process feasibility and the cell protection effect is needed. In the prior published literature and patent, for example, publication number (China patent of CN117378764A discloses a probiotic microcapsule, a preparation method and application thereof, which adopts a traditional core material-wall material single-layer gel embedding structure, forms a gel network under the action of a cross-linking agent through materials such as sodium alginate, chitosan and the like, wraps probiotics, the protection effect of the probiotic is mainly dependent on a single physical barrier, the wall material is dependent on the compounding of natural polysaccharide such as sodium alginate, chitosan and the like, and calcium carbonate is needed to be added as the cross-linking agent, span emulsifying agent and vegetable oil containing acetic acid as the inducer, so that the raw material system is complex. Disclosure of Invention The invention aims to provide a probiotic microcapsule, and a preparation method and application thereof. The invention constructs a double protection structure through porous starch and cellulose derivatives, and utilizes a microencapsulation technology combining physical adsorption and embedding to form a protection barrier with high encapsulation efficiency, heat resistance, pressure resistance and gastrointestinal digestion resistance, thereby realizing high survival rate o