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CN-122004479-A - Nutritional food containing small molecule active peptide and preparation method thereof

CN122004479ACN 122004479 ACN122004479 ACN 122004479ACN-122004479-A

Abstract

The invention discloses a nutritional food containing small molecular active peptide, which relates to the technical field of health care food, and comprises, by weight, 15-35 parts of deer blood small molecular active peptide, 10-30 parts of a grass concentrated extract, 15-40 parts of whey protein, 5-20 parts of phycocyanin, 3-15 parts of collagen peptide and 2-10 parts of glucan. Through a special preparation process, macromolecular nutrient substances are converted into micromolecular forms, such as deer blood micromolecular active peptide and low-molecular-weight collagen peptide, the bioavailability of the micromolecular nutrient substances is greatly improved, the micromolecular nutrient substances are easier to digest and absorb by a human body, and compared with the traditional macromolecular nutrient food, the nutrient food disclosed by the invention can be absorbed and utilized by the human body more quickly.

Inventors

  • CHEN GUANZHU
  • ZHAO MEI
  • MA LEI
  • MAO YUANZHE

Assignees

  • 河南城建学院

Dates

Publication Date
20260512
Application Date
20260407

Claims (9)

  1. 1. A nutritional food containing small molecular active peptide is characterized by comprising, by weight, 15-35 parts of deer blood small molecular active peptide, 10-30 parts of a phyllanthus niruri concentrated extract, 15-40 parts of whey protein, 5-20 parts of phycocyanin, 3-15 parts of collagen peptide and 2-10 parts of glucan.
  2. 2. A nutritional food product comprising small molecule active peptides according to claim 1 wherein the average molecular weight of said deer blood small molecule active peptides is between 100 and 800 daltons.
  3. 3. The nutritional food containing the small molecule active peptide of claim 1, wherein the concentrated extract of phyllanthus niruri has a concentration ratio of 15-40:1.
  4. 4. A nutritional food product comprising a small molecule active peptide according to claim 1 wherein the collagen peptide has a molecular weight of between 200 and 1000 daltons.
  5. 5. A method for preparing a nutritional food containing a small molecular active peptide according to any one of claims 1 to 4, comprising the steps of: Step one, drying and grinding fresh deer blood into deer blood powder, mixing the deer blood powder and distilled water according to a weight ratio of feed liquid of 1:8-1:20, performing ultrasonic treatment with ultrasonic power of 120-180W for 25-50min, and performing constant-temperature vibration for 70-110min to obtain deer blood solution; Adding acid protease into deer blood solution, regulating the pH to 2.2-2.8 by using 1.0mol/L HCl, and carrying out enzymolysis for 1.5-7.0h in a constant-temperature oscillating water bath kettle at 37-62 ℃ to maintain the pH stable in the enzymolysis process; Regulating the pH to 7.2-7.8 by using 1.0mol/L NaOH, adding neutral protease, carrying out enzymolysis for 1.5-7.0h in a constant-temperature oscillating water bath kettle at 37-62 ℃, maintaining the pH stable in the enzymolysis process, inactivating enzyme at high temperature in a magnetic stirrer at 92-98 ℃ for 8-25min after enzymolysis, centrifuging for 12-25min in a centrifuge at 8200-9300rpm after cooling, and extracting supernatant; Sequentially adding beta-cyclodextrin and yeast into the supernatant to perform mixed deodorization, wherein the mass ratio of the beta-cyclodextrin to the deer blood small molecular active peptide is 2.5% -3.5%, the deodorization is performed for 40-100min at 32-55 ℃, the mass ratio of the yeast to the deer blood small molecular active peptide is 0.8% -1.5%, the deodorization is performed for 40-100min at 32-45 ℃, centrifuging to collect the supernatant, pre-freezing for 15-40h, and freeze-drying to obtain deer blood small molecular active peptide freeze-dried powder; Step five, cleaning the phyllanthus niruri, drying at low temperature until the moisture content is lower than 8%, crushing, sieving with a 60-100 mesh sieve, extracting with water or ethanol as a solvent for 2-4 times at 50-80 ℃ for 1-3h each time, combining the extracting solutions, finely filtering, concentrating under reduced pressure, concentrating again to obtain a fluid extract, spray-drying, crushing, and sieving with a 80-120 mesh sieve to obtain a phyllanthus niruri concentrated extract; Step six, mixing the deer blood micromolecular active peptide freeze-dried powder, the phyllanthus urinaria concentrated extract, the whey protein, the phycocyanin, the collagen peptide and the dextran according to a proportion, adding a flavoring agent and a stabilizing agent, adding a proper amount of purified water, and uniformly stirring to prepare corresponding formulations.
  6. 6. The method for preparing a nutritional food containing small molecule active peptide according to claim 5, wherein the mass of the acidic protease is 0.4% -0.8% of that of deer blood powder, and the mass ratio of neutral protease to acidic protease is 1-4:1.
  7. 7. The method for preparing a nutritional food containing small molecule active peptides according to claim 5, wherein the low temperature drying temperature of the phyllanthus niruri is 40-60 ℃.
  8. 8. The method for preparing a nutritional food containing small molecule active peptide according to claim 5, wherein the preparation is oral liquid, and is prepared by filtering with 0.22 μm filter membrane, packaging, sterilizing, and drying, granulating, and packaging.
  9. 9. The method of claim 5, wherein the flavoring agent is one or more of a sweetener and a sour agent, and the stabilizer is one or more of pectin and sodium carboxymethyl cellulose.

Description

Nutritional food containing small molecule active peptide and preparation method thereof Technical Field The invention relates to the technical field of health-care food, in particular to a nutritional food containing small molecular active peptide and a preparation method thereof. Background With the increase of health consciousness, the demands for nutritional foods are increasingly diversified and refined. Conventional nutritional foods suffer from a number of deficiencies in the integrity, absorption and utilization of nutritional ingredients, and in their functional properties. On the one hand, common nutritional foods are often focused on supplementing only a single type of nutrients, such as protein or vitamins, and are difficult to meet the requirement of human body on the synergistic effect of multiple nutritional ingredients. On the other hand, the technical limitation in the food processing process leads the nutrition components to be easy to lose or lose in the processing process, and reduces the nutritive value of the product. For example, in some protein nutritional products, macromolecular proteins are not easily digested and absorbed by the human body, and conditions such as high temperatures during processing can cause protein denaturation, affecting its biological activity. In addition, the existing nutritional food has unsatisfactory effects in coping with specific health requirements of human body, such as enhancing immunity, resisting oxidation, repairing damaged cells, etc. The invention discloses a preparation method of deer blood polypeptide oral liquid with immunoregulation function, which improves the hydrolysis degree and the hydrolysis effect of deer blood polypeptide by using neutral protease and acid protease for enzymolysis, solves the problem of fishy smell of deer blood polypeptide products by adding beta-cyclodextrin and yeast, and solves the problem of instability of the products by adding complex colloid. However, the product mainly uses deer blood polypeptide as a core component, and is matched with a small amount of auxiliary materials, so that the nutritional ingredients are relatively single. Disclosure of Invention Aiming at the defects of the prior art, the invention provides a nutritional food containing small molecular active peptide and a preparation method thereof, and solves the problems of the prior art. The invention aims at realizing the purposes by adopting the following technical scheme that the nutritional food containing the micromolecular active peptide consists of, by weight, 15-35 parts of deer blood micromolecular active peptide, 10-30 parts of a phyllanthus niruri concentrated extract, 15-40 parts of whey protein, 5-20 parts of phycocyanin, 3-15 parts of collagen peptide and 2-10 parts of glucan. Preferably, the average molecular weight of the deer blood small molecule active peptide is between 100 and 800 daltons. Preferably, the concentration and extraction ratio of the phyllanthus niruri concentrated extract is 15-40:1. Preferably, the collagen peptide has a molecular weight between 200 and 1000 daltons. The invention also discloses a preparation method of the nutritional food containing the small molecular active peptide, which comprises the following steps: Step one, drying and grinding fresh deer blood into deer blood powder, mixing the deer blood powder and distilled water according to a weight ratio of feed liquid of 1:8-1:20, performing ultrasonic treatment with ultrasonic power of 120-180W for 25-50min, and performing constant-temperature vibration for 70-110min to obtain deer blood solution; Adding acid protease into deer blood solution, regulating the pH to 2.2-2.8 by using 1.0mol/L HCl, and carrying out enzymolysis for 1.5-7.0h in a constant-temperature oscillating water bath kettle at 37-62 ℃ to maintain the pH stable in the enzymolysis process; Regulating the pH to 7.2-7.8 by using 1.0mol/L NaOH, adding neutral protease, carrying out enzymolysis for 1.5-7.0h in a constant-temperature oscillating water bath kettle at 37-62 ℃, maintaining the pH stable in the enzymolysis process, inactivating enzyme at high temperature in a magnetic stirrer at 92-98 ℃ for 8-25min after enzymolysis, centrifuging for 12-25min in a centrifuge at 8200-9300rpm after cooling, and extracting supernatant; Sequentially adding beta-cyclodextrin and yeast into the supernatant to perform mixed deodorization, wherein the mass ratio of the beta-cyclodextrin to the deer blood small molecular active peptide is 2.5% -3.5%, the deodorization is performed for 40-100min at 32-55 ℃, the mass ratio of the yeast to the deer blood small molecular active peptide is 0.8% -1.5%, the deodorization is performed for 40-100min at 32-45 ℃, centrifuging to collect the supernatant, pre-freezing for 15-40h, and freeze-drying to obtain deer blood small molecular active peptide freeze-dried powder; Step five, cleaning the phyllanthus niruri, drying at low temperature until the moisture con