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CN-122004484-A - Method for synergistically increasing resistant starch by autoclave treatment and polyphenol

CN122004484ACN 122004484 ACN122004484 ACN 122004484ACN-122004484-A

Abstract

The invention belongs to the technical field of food processing, and particularly relates to a method for synergistically increasing resistant starch by autoclave treatment and polyphenol. The invention optimizes the autoclave process of rice starch by a response surface method, and obtains the optimal conditions of 43% of moisture content, 92 ℃ of autoclave temperature and 17 min of autoclave time, and the RS content can reach (22.22+/-0.23)%. On the basis, the digestion characteristic of starch can be further regulated by adding tea polyphenol, wherein the effects of TFs and EGCG on reducing RDS and improving RS are obviously superior to DTE.

Inventors

  • SHI MENG
  • XIANG YUXIN
  • WU YUANJIE

Assignees

  • 湖南农业大学

Dates

Publication Date
20260512
Application Date
20260116

Claims (9)

  1. 1. A method for synergistically increasing resistant starch by autoclave treatment and polyphenol is characterized in that the method comprises the steps of mixing rice starch and polyphenol, and then performing autoclave treatment, wherein the polyphenol is EGCG or TFs.
  2. 2. The method of claim 1 wherein said TFs comprise four monomers of theaflavin, theaflavin-3-gallate, theaflavin-3' -gallate and theaflavin digallate.
  3. 3. The method according to claim 2, wherein the mass fraction of polyphenols is 3-5% of rice starch.
  4. 4. A method according to claim 3, wherein the autoclave time is 5-25min.
  5. 5. The method of claim 4, wherein the autoclave moisture content is 40-60%.
  6. 6. The method of claim 5, wherein the autoclave temperature is 80-100 ℃.
  7. 7. The method of claim 6, wherein the autoclave time is 17 minutes, the autoclave moisture content is 43%, and the autoclave temperature is 92 ℃.
  8. 8. The method of claim 2, wherein the total mass fraction of theaflavin, theaflavin-3-gallate, theaflavin-3' -gallate and theaflavin digallate in TFs is 44.12%.
  9. 9. The method of claim 1, wherein the mass fraction of EGCG is 95%.

Description

Method for synergistically increasing resistant starch by autoclave treatment and polyphenol Technical Field The invention belongs to the technical field of food processing, and particularly relates to a method for synergistically increasing resistant starch by autoclave treatment and polyphenol. Background Rice is a major food crop in the global area, the digestion characteristics of starch can directly act on postprandial blood glucose reaction and metabolic health of a human body, and the digestion rate of starch is mainly determined by the multi-scale structural characteristics of the rice. Different processing methods can adjust the structure of starch and influence the digestibility of starch, and reducing the digestibility of starch by using different processing methods has become an important research direction for developing low Glycemic Index (GI) foods. The pressure heat treatment mode is used as a physical modification means with obvious effect, and the process can induce the structural transformation of starch such as gelatinization, recrystallization and the like by controlling parameters such as moisture, temperature, time and the like and promote the formation of resistant starch (RS 3 type), thereby obviously delaying the digestion of starch. LIU et al used ultrasonic combined with autoclave to prepare potato resistant starch increased the yield of resistant starch (RESISTANT STARCH, RS) from 7.5% to 15.9%. Related researches also show that the heat treatment of the millet by pressing can raise the RS production to about 30.64%. The digestion characteristics of the starch are regulated by means of natural polyphenols, and the polyphenols can form a compound with a starch molecular chain by means of non-covalent bonds such as hydrogen bonds and hydrophobic interactions and obviously change the physicochemical properties, the multi-scale structure and the digestion behavior of the starch. Researches show that polyphenols such as epigallocatechin gallate ((-) -epigallocatechin-3-gallate, EGCG), quercetin and the like can effectively reduce the content of fast digestion starch (rapidly digestible starch, RDS) of rice starch and remarkably improve the contents of Resistant Starch (RS) and slow digestion starch (slowly digestible starch, SDS). However, the current research focuses on the separate effects of autoclave treatment or polyphenol addition, and the effect of the synergy of the two on starch digestibility is not clear. Therefore, the invention aims to systematically explore the synergistic effect of the autoclave process and different polyphenols (EGCG, theaflavins (theaflavins, TFs) and black tea extracts) and clarify the influence mechanism of the synergistic effect on starch digestibility, and aims to provide theoretical basis and technical support for the development of low GI functional rice products. Disclosure of Invention In order to obtain a method for increasing the content of resistant starch after rice starch treatment, the invention discloses the following technical scheme: a method for synergistically increasing resistant starch by autoclave treatment and polyphenol comprises the steps of mixing rice starch with polyphenol, and then performing autoclave treatment, wherein the polyphenol is EGCG or TFs. Preferably, the TFs comprise four monomers of theaflavin, theaflavin-3-gallate, theaflavin-3' -gallate and theaflavin digallate. Preferably, the mass fraction of the polyphenol is 3-5% of the rice starch. Preferably, the autoclave time is 5-25min. Preferably, the autoclave moisture content is 40-60%. Preferably, the autoclave temperature is 80-100 ℃. Preferably, the autoclave time is 17min, the content of the autoclave water is 43%, and the autoclave temperature is 92 ℃. Preferably, the total mass fraction of theaflavin, theaflavin-3-gallate, theaflavin-3' -gallate and theaflavin digallate in the TFs is 44.12%. Preferably, the mass fraction of the EGCG is 95%. The invention has the beneficial effects that: The invention optimizes the autoclave process of rice starch by a response surface method, and obtains the optimal conditions of 43% of moisture content, 92 ℃ of autoclave temperature and 17 min of autoclave time, and the RS content can reach (22.22+/-0.23)%. On the basis, the digestion characteristic of starch can be further regulated by adding tea polyphenol, wherein the effects of TFs and EGCG on reducing RDS and improving RS are obviously superior to DTE. Drawings FIG. 1 is a graph showing the effect of moisture content on resistant starch content, note that the different lower case letters indicate significant differences between groups (P < 0.05), the same applies below; FIG. 2 is the effect of autoclave temperature on resistant starch content; FIG. 3 is an effect of autoclave time on resistant starch content; FIG. 4 is a graph showing the effect of TFs addition on rice starch digestibility; FIG. 5 is a graph showing the effect of EGCG addition on rice starch digestib