CN-122004485-A - Preparation method of ultrahigh-pressure micro-jet homogenizing-snailase modified bean dreg insoluble dietary fiber

CN122004485ACN 122004485 ACN122004485 ACN 122004485ACN-122004485-A

Abstract

The invention provides bean dreg insoluble dietary fiber and a preparation method thereof, and belongs to the technical field of bean dreg recycling deep processing. The preparation method of the bean dreg insoluble dietary fiber comprises the following steps of degreasing bean dreg to obtain defatted bean dreg powder, fully mixing the defatted bean dreg powder with deionized water, carrying out ultrahigh-pressure micro-jet homogenization treatment to obtain bean dreg powder-water suspension, carrying out high-temperature resistant alpha-amylase enzymolysis, neutral protease enzymolysis and amyloglucosidase enzymolysis on the bean dreg powder-water suspension in sequence, carrying out enzyme deactivation, carrying out snailase enzymolysis, carrying out enzyme deactivation, and drying to obtain the bean dreg insoluble dietary fiber. According to the invention, the bean dreg insoluble dietary fiber is prepared by adopting the ultrahigh-pressure micro-jet homogenization and the enzymatic modification, so that the insoluble dietary fiber with high yield and high content can be obtained, the dual promotion of the added value and the industrialization potential of bean dreg byproducts is realized, and an important technical guarantee is provided for the large-scale application of the bean dreg insoluble dietary fiber in the field of functional foods.

Inventors

Zheng huanyu
WEI XUYAO
YANG JIAYAO
LI DAN

Assignees

东北农业大学

Dates

Publication Date: 20260512
Application Date: 20260323

Claims (7)

1. A preparation method of bean dreg insoluble dietary fiber is characterized by comprising the steps of degreasing bean dreg to obtain defatted bean dreg powder, fully mixing the defatted bean dreg powder with deionized water, carrying out ultrahigh pressure micro-jet homogenization treatment to obtain bean dreg powder-water suspension, carrying out high temperature resistant alpha-amylase enzymolysis, neutral protease enzymolysis and amyloglucosidase enzymolysis on the bean dreg powder-water suspension in sequence, carrying out enzyme deactivation, carrying out helicase enzymolysis, then carrying out enzyme deactivation and drying to obtain the bean dreg insoluble dietary fiber, wherein the working pressure of the ultrahigh pressure micro-jet homogenization treatment is 200-250 MPa, and the treatment time is 20-25 min.
2. The preparation method of the high-temperature resistant alpha-amylase, which is characterized in that the enzyme adding amount of the high-temperature resistant alpha-amylase is 1% -1.5% of the high-temperature resistant alpha-amylase added per 50g of the bean dreg mixture, the enzyme activity of the high-temperature resistant alpha-amylase is 2X 104U/g, the temperature of the high-temperature resistant alpha-amylase is 75-95 ℃, the time is 1-1.5 h, and the pH is 5.0-8.0.
3. The preparation method of the soybean milk powder is characterized in that the enzyme adding amount of the neutral protease is 2% -2.5% of the neutral protease added to each 50g of soybean milk powder mixture, the enzyme activity of the neutral protease is 5X 104U/g, the enzymolysis temperature of the neutral protease is 35-55 ℃, the enzymolysis time is 2-2.5 h, and the pH is 6.5-8.0.
4. The preparation method of the amyloglucosidase, which is characterized in that the enzyme adding amount of the amyloglucosidase is 1% -1.5% of the amyloglucosidase added to 50g of the bean dreg mixture, the enzyme activity of the amyloglucosidase is 2X 104U/g, the temperature of the amyloglucosidase is 50-65 ℃, the time is 1-1.5 h, and the pH is 4.0-5.5.
5. The preparation method of the snail enzyme according to claim 1, wherein the enzyme adding amount of snail enzyme enzymolysis is 1% -3% of snail enzyme added to each 50g of bean dreg mixture, the enzyme activity of the snail enzyme is 3U/mg, the temperature of snail enzyme enzymolysis is 40-60 ℃, the time is 1-4 h, and the pH is 4.0-7.5.
6. The preparation method of claim 1, wherein the degreasing raw material is petroleum ether, the degreasing feed liquid ratio is 1g of bean dregs and 10-15 mL of petroleum ether, and the degreasing time is 5-10 h.
7. The okara insoluble dietary fiber produced by the production method of any one of claims 1 to 6.

Description

Preparation method of ultrahigh-pressure micro-jet homogenizing-snailase modified bean dreg insoluble dietary fiber Technical Field The invention belongs to the technical field of bean dreg recycling deep processing, and particularly relates to bean dreg insoluble dietary fiber and a preparation method thereof. Background Dietary Fiber (DF) is a carbohydrate polymer with a Degree of Polymerization (DP) of 3 or more, which cannot be digested and absorbed by the human small intestine but has a positive effect on health. The nutrient can be derived from natural plants or obtained by extraction or artificial synthesis, and the main components of the nutrient comprise cellulose, hemicellulose, pectin and other polysaccharide substances, so that the nutrient is known as a seventh nutrient. Dietary fibers can be divided into two categories, soluble dietary fibers (Soluble Dietary Fiber, SDF) and insoluble dietary fibers (Insoluble Dietary Fiber, IDF) depending on solubility. Insoluble dietary fibers regulate intestinal function in the gastrointestinal system mainly by physical action, as compared to soluble dietary fibers. The multifunctional intestinal tract peristalsis medicine can increase the volume of excrement, promote intestinal tract peristalsis, effectively prevent and relieve constipation, adsorb and discharge harmful substances, and is beneficial to maintaining colon health and reducing colorectal cancer risk. In addition, it can enhance satiety, assist weight management and blood glucose stabilization, and is a key dietary component for maintaining digestive tract health. The bean dregs are typical byproducts in the processing process of the soybean products, are solid substances remained after the preparation of soybean milk or bean curd, and mainly comprise soybean cell walls, proteins and carbohydrates which are not completely extracted. It contains rich dietary fiber, high-quality plant protein, various minerals and physiological active components essential to human body. The bean dregs are good bioactive substance sources, and have remarkable effects in promoting intestinal peristalsis, assisting in regulating blood sugar and blood fat, enhancing satiety to control weight, reducing cholesterol, improving organism immunity, preventing chronic diseases and the like. Therefore, the directional modification treatment is carried out on the bean dregs, so that the content and the functional characteristics of insoluble dietary fibers are obviously improved, the method breaks through the bottleneck of resource utilization and realizes a key path of high-value fine and further processing, and has important significance for promoting the industrial upgrading and sustainable utilization of bean-based byproducts. The ultrahigh pressure micro-jet homogenizing treatment drives the material to pass through a micro channel to generate physical effects such as shearing, impact, cavitation and the like, and can destroy the compact structure and intermolecular acting force of the material, thereby inducing various physical and chemical changes. The ultrahigh pressure micro-jet homogenization treatment is a green, efficient and mild technology, can well retain active ingredients while realizing material modification, does not introduce chemical additives, and has been successfully applied to extraction and structural optimization of high added value ingredients in various natural products in recent years. Meanwhile, snailase is taken as a compound enzyme preparation, and can effectively hydrolyze cell wall polysaccharide and impurities, thus showing unique advantages in the aspects of improving the fiber purity and improving the functional characteristics. At present, the research on directional modification of insoluble dietary fibers of bean dregs by the synergistic effect of ultrahigh-pressure micro-jet homogenization treatment and an enzymatic method is reported, so that the innovation of the synergistic strategy is expected to provide a new path for the high-value development of bean dregs. Disclosure of Invention In view of the above, the invention aims to provide a preparation method of bean dreg insoluble dietary fiber, which adopts a preparation process of an ultrahigh pressure micro-jet homogenization combined enzyme method, has the advantages of efficient modification, green safety and high yield, and is suitable for preparing raw materials in the fields of functional foods, special medical purpose formula foods and the like. In order to achieve the above object, the present invention provides the following technical solutions: The invention provides a preparation method of bean dreg insoluble dietary fiber, which comprises the following steps of degreasing bean dreg to obtain defatted bean dreg powder, fully mixing the defatted bean dreg powder with deionized water, carrying out ultrahigh pressure micro-jet homogenization treatment to obtain bean dreg powder-water suspension, carrying out high temperatur