CN-122004754-A - Wireless monitoring method, system and monitoring equipment for calcium imaging of laboratory mice

CN122004754ACN 122004754 ACN122004754 ACN 122004754ACN-122004754-A

Abstract

The invention discloses a wireless monitoring method, a wireless monitoring system and a wireless monitoring device for calcium imaging of an experimental mouse, wherein the method comprises the steps of arranging an optical detection structure beside a labeling area of an intracranial calcium indicator of the experimental mouse, and capturing fluorescent signals; the method comprises the steps of constructing a calcium imaging original data mapping model, converting fluorescent signals into initial calcium imaging data, obtaining pure calcium imaging data through self-adaptive filtering and noise reduction, carrying out lightweight compression based on time sequence periodic characteristics, packaging transmission data through a low-power consumption wireless transmission model with a plurality of groups of preset independent channels, decompressing an external receiving terminal, generating visual information through a calcium imaging reduction model, integrating optical detection structures, signal processors, wireless transmitters and other core components according to the method, and thoroughly getting rid of long optical fiber constraint, has microminiaturization, low power consumption, interference resistance and good biocompatibility, ensures the accuracy and experimental continuity of monitoring data, and is suitable for the brain activity related research of experimental mice.

Inventors

LUO YANAN
HUANG YAGUANG
LU CHAO
XU JIANGFENG
OU XIA

Assignees

三峡大学

Dates

Publication Date: 20260512
Application Date: 20260114

Claims (10)

1. The low-power-consumption anti-interference experimental mouse calcium imaging wireless monitoring method is characterized by comprising the following steps of: An optical detection structure is arranged beside an intracranial calcium indicator marking area of the experimental mouse, and exciting light is emitted to the intracranial calcium indicator marking area so as to trigger the calcium indicator to release a fluorescent signal and capture the fluorescent signal; Based on the corresponding relation between the fluorescence signal and the calcium ion concentration, constructing a calcium imaging original data mapping model, and converting the fluorescence signal into initial calcium imaging data; Noise reduction pretreatment is carried out on the initial calcium imaging data, and an adaptive filtering algorithm is adopted to eliminate the ambient light interference and the electronic noise, so that pure calcium imaging data are obtained; Based on time sequence periodic characteristics of pure calcium imaging data, constructing a lightweight data compression model, and compressing the pure calcium imaging data; Constructing a low-power-consumption wireless transmission model, and transmitting compressed calcium imaging data to an external receiving terminal after being packaged according to a preset frame format through preset groups of independent transmission channels; And after the external receiving terminal receives the compressed calcium imaging data, a data decompression model is called to decompress the compressed calcium imaging data, and the decompressed data is converted into visual calcium imaging information through a calcium imaging reduction model to complete calcium imaging monitoring.
2. The low-power-consumption anti-interference experimental mouse calcium imaging wireless monitoring method of claim 1, wherein the constructing a calcium imaging raw data mapping model based on the corresponding relation between fluorescent signals and calcium ion concentration specifically comprises: the fluorescent signal intensity generated by the calcium indicator under different calcium ion concentrations is measured through experiments, and the calcium ion concentration is established And fluorescence signal intensity The formula is: , wherein, Is the basic fluorescence signal intensity of the calcium indicator in the environment without calcium ions, Is the coefficient of the change of fluorescence signal intensity along with the concentration of calcium ions; Based on constructing a calcium imaging original data mapping model, the fluorescence signal intensity acquired in real time is obtained Substituting and calculating to obtain the corresponding calcium ion concentration And will As initial calcium imaging data.
3. The low-power-consumption anti-interference experimental mouse calcium imaging wireless monitoring method according to claim 1, wherein the noise reduction pretreatment of initial calcium imaging data by adopting an adaptive filtering algorithm comprises the following steps: setting the size of the adaptive filter window to be In a time series of initial calcium imaging data For processing object, calculating average value of data in filtering window And standard deviation The formula is: , the mean value of the deviation in the window exceeds the deviation threshold value The data of (2) is determined to be noise data, and the noise data is replaced by the mean value of non-noise data in a window to obtain noise-reduced pure calcium imaging data 。
4. The low-power-consumption anti-interference experimental mouse calcium imaging wireless monitoring method of claim 1, wherein the constructing a lightweight data compression model based on the time sequence periodic characteristics of pure calcium imaging data specifically comprises: Imaging data on pure calcium Performing time sequence analysis, extracting period of data by Fourier transformation Determining a periodic repetition rule of the data; constructing a lightweight data compression model for one period Pure calcium imaging data within The complete reservation is carried out, and for the data in the subsequent period, only the difference value of the time data corresponding to the previous period is stored The formula is: , wherein, Is the first Time of day in each period Is used for the pure calcium imaging data of the (a), Is the first Time of day in each period The compression processing of the pure calcium imaging data is completed in the mode.
5. The method for wireless monitoring of low-power-consumption anti-interference experimental mouse calcium imaging according to claim 1, wherein the constructing of the low-power-consumption wireless transmission model presets a plurality of groups of independent transmission channels, and specifically comprises: Constructing a low-power-consumption wireless transmission model and presetting Groups of independent transmission channels, each group of channels corresponding to a different center frequency Model real-time detection of signal interference intensity of each channel Setting an interference intensity threshold Will be When there are multiple idle channels, the model calculates the transmission rate of each idle channel And selecting an idle channel with the maximum transmission rate as a data transmission channel.
6. The method for wireless monitoring of calcium imaging of experimental mouse with low power consumption and anti-interference as set forth in claim 1, wherein the method comprises packaging the compressed calcium imaging data in a predetermined frame format and adding check codes during the packaging process of the data frame, and specifically comprises setting a data frame format comprising a frame header, a data segment, a check code segment and a frame tail, wherein the frame header is used for identifying the beginning of the data frame, the data segment is used for storing the compressed calcium imaging data, the check code segment is used for storing the check codes, the frame tail is used for identifying the end of the data frame, and calculating the check codes of the compressed calcium imaging data by adopting a cyclic redundancy check algorithm The formula is: , wherein, For the compressed calcium imaging data, Filling the compressed calcium imaging data into a data segment, filling the calculated check code into a check code segment, and completing the encapsulation of the data frame.
7. The low-power-consumption anti-interference experimental mouse calcium imaging wireless monitoring method of claim 1, wherein the external receiving terminal invokes a data decompression model to decompress compressed calcium imaging data, and the method specifically comprises the following steps: the external receiving terminal receives the encapsulated data frame and extracts the compressed calcium imaging data in the data segment And check code in the check code segment Calling a data decompression model based on the check code Verification If the verification is passed, the model extracts a period Internal integrity pure calcium imaging data Based on the stored difference By recovering pure calcium imaging data of a subsequent period, the formula is: and (3) completing decompression processing of the compressed calcium imaging data to obtain a complete pure calcium imaging data sequence.
8. The low-power-consumption anti-interference experimental mouse calcium imaging wireless monitoring method according to claim 1, wherein the method is characterized in that decompressed data are converted into visual calcium imaging information through a calcium imaging reduction model, and specifically comprises the following steps: Constructing a calcium imaging reduction model, and setting a mapping relation between the concentration of calcium ions and the gray value of an image pixel, wherein the formula is as follows: , wherein, For the gray value of a pixel of an image, Is a proportionality coefficient of gray value changing along with the concentration of calcium ions, The calcium ion concentration obtained after decompression is used as a basic gray level value Substituting and calculating to obtain corresponding pixel gray value According to the spatial distribution of the marked area of the intracranial calcium indicator of the experimental mouse, the gray value of the pixel corresponding to each position is obtained And performing space mapping to generate a two-dimensional or three-dimensional visual calcium imaging image, and finishing the restoration of calcium imaging information.
9. The low-power-consumption anti-interference experimental mouse calcium imaging wireless monitoring system is characterized by comprising a fluorescent signal acquisition unit, an original data mapping unit, a data processing unit, a wireless transmission unit, a data decompression unit and a calcium imaging reduction unit; The output end of the fluorescence signal acquisition unit is connected with the input end of the original data mapping unit, acquires a fluorescence signal generated by exciting the intracranial calcium indicator of the laboratory mouse, and transmits the fluorescence signal to the original data mapping unit; The output end of the original data mapping unit is connected with the input end of the data noise reduction unit, a calcium imaging original data mapping model is constructed based on the corresponding relation between the fluorescence signal and the calcium ion concentration, the fluorescence signal is converted into initial calcium imaging data, and the initial calcium imaging data is transmitted to the data noise reduction unit; The data processing unit is used for carrying out noise reduction pretreatment on initial calcium imaging data by adopting a self-adaptive filtering algorithm to obtain pure calcium imaging data, constructing a lightweight data compression model based on time sequence periodic characteristics of the pure calcium imaging data, carrying out compression treatment on the pure calcium imaging data, and transmitting the compressed calcium imaging data to the wireless transmission unit; The wireless transmission unit is in wireless connection with an external receiving end and is used for constructing a low-power consumption wireless transmission model, packaging the compressed calcium imaging data according to a preset frame format, adding a check code, and transmitting the data to the receiving end through a selected idle channel; The input end of the data decompression unit is connected with the receiving end, the output end of the data decompression unit is connected with the input end of the calcium imaging reduction unit, and the data decompression unit is used for calling a data decompression model to decompress received compressed calcium imaging data to obtain complete pure calcium imaging data and transmitting the pure calcium imaging data to the calcium imaging reduction unit; the output end of the calcium imaging reduction unit is connected with the input end of the display storage unit, and the output end of the calcium imaging reduction unit is used for converting decompressed data into visual calcium imaging information through a calcium imaging reduction model, so that wireless monitoring of experimental mouse calcium imaging is realized.
10. A low-power-consumption anti-interference experimental mouse calcium imaging wireless monitoring device for realizing the method of claims 1-8, which is characterized by comprising a head integrated component, a receiving terminal and terminal equipment; The head integrated component is attached to the head of the experimental mouse and is internally integrated with an optical detection structure, a signal processor and a wireless transmitter, wherein the optical detection structure comprises a micro excitation light source and a micro fluorescence detector, the micro excitation light source emits excitation light rays to excite an intracranial calcium indicator to generate a fluorescence signal, and the micro fluorescence detector collects the fluorescence signal and transmits the fluorescence signal to the signal processor; after receiving the compressed data, the receiving terminal decompresses the compressed data and transmits the decompressed data to the terminal equipment; The terminal equipment comprises a data processor and a display memory, wherein the data processor converts decompressed calcium imaging data into visual calcium imaging information, and the display memory displays the information in real time and stores the information to realize wireless monitoring of experimental mouse calcium imaging.

Description

Wireless monitoring method, system and monitoring equipment for calcium imaging of laboratory mice Technical Field The invention relates to the technical field of calcium imaging monitoring, in particular to a low-power-consumption anti-interference wireless monitoring method, system and monitoring equipment for calcium imaging of an experimental mouse. Background Calcium imaging is one of the core technologies for researching brain activities of laboratory mice in the fields of neuroscience, physiology and the like, and converts the electrical activities of nerve cells into detectable fluorescent signals through the specific binding reaction of a calcium indicator and calcium ions, so that the dynamic monitoring of the functions of specific areas of the brain is realized. The traditional calcium imaging monitoring relies on an optical fiber recorder, the equipment needs to penetrate through an intracranial and external detection system of an experimental mouse through a long optical fiber, and the optical fiber simultaneously bears the dual functions of excitation light transmission and fluorescence signal derivation, so that the equipment is a core carrier for signal transmission in early calcium imaging technology. In basic research, the technology provides key data support for revealing experimental mouse brain nerve loop connection, behavioural association mechanism and the like, and is widely applied to a plurality of leading directions of neurodegenerative disease model research, drug action target screening, cognitive function analysis and the like, and becomes an indispensable tool in life science research. The long optical fiber design of the traditional optical fiber recorder has a plurality of technical defects which are difficult to overcome, and the accuracy and the practicability of the experiment are seriously restricted. In addition, the optical fiber is hard in material and long in length, and when the mice move in the raising cage, the optical fiber is easy to wind and bend, so that normal feeding, hair combing, movement and other behaviors of the mice are limited, and the intracranial tissue injury is caused by mechanical pulling, thereby disturbing the physiological state of the mice. More critical, the mouse has the potential of biting foreign matters, the exposed part of the long optical fiber is extremely easy to be bitten and damaged by the mouse, so that signal transmission is interrupted, experimental data is directly lost, and the optical fiber is implanted in a re-operation mode after being damaged, so that the experimental cost is increased, secondary damage is caused to the experimental mouse, and the consistency of a sample is affected. Besides the problems of fixation and biting, the long optical fiber transmission mode also has the problems of poor signal stability and limited experimental scene. On one hand, the long optical fiber can lead to signal attenuation due to optical fiber loss, scattering and the like in the process of transmitting excitation light and fluorescent signals, especially the intensity of the fluorescent signals is weaker, the signal to noise ratio is obviously reduced after long-distance transmission, and the accuracy of calcium ion concentration detection is affected, on the other hand, the physical constraint of the optical fiber enables an experimental mouse to be incapable of deviating from the limited range of an external detection system, so that a monitoring experiment in a long-term free activity state is difficult to develop, and brain activity data in a natural behavior state is vital to the authenticity of a research result. In addition, the external detection system of the traditional optical fiber recorder is huge in size and needs to be fixedly placed in an experimental environment, so that not only is space occupied, but also electromagnetic interference generated during operation of the external detection system further influences signal detection quality, and a plurality of inconveniences are brought to experimental design and operation. Along with the development of life science research to the direction of refinement, long-term and dry pretreatment, the limitation of the traditional optical fiber recorder is more and more prominent, and the demands of scientific researchers on novel calcium imaging monitoring technology are more and more urgent. The existing wireless monitoring technology is applied to some fields, but in the experimental mouse calcium imaging scene, the multiple technical challenges of miniature integration, low-power consumption endurance, biocompatibility and signal transmission stability are faced. The experimental mouse has small size, light weight and small volume, and needs the head carrying device to avoid influencing normal physiological activities, and meanwhile, the long-term monitoring requirement provides requirements for the endurance capacity of the device, and the balance between the low-power consu