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CN-122004776-A - Three-dimensional fluorescence tomography device

CN122004776ACN 122004776 ACN122004776 ACN 122004776ACN-122004776-A

Abstract

A three-dimensional fluorescence tomography device comprises a shooting part, an image processing part, a plurality of excitation light emitting parts, a tomography light emitting part, a first reflecting mirror, a beam splitter, a second reflecting mirror, a multi-wavelength light source part and a wavelength adjustable filter, wherein the image processing part respectively extracts a first separation image corresponding to a first fluorescent developer and a second separation image corresponding to a second fluorescent developer from each fluorescent image, calculates the depth corresponding to each first separation image and each second separation image according to depth information in an optical coherence tomography image, and combines the first separation image and the second separation image according to the depth of each first separation image and the second separation image to respectively form a three-dimensional fluorescence tomography image corresponding to the first fluorescent developer and a three-dimensional fluorescence tomography image corresponding to the second fluorescent developer of a shooting object.

Inventors

  • HAN YINGGEN
  • WANG DONGMEI
  • He Huishu
  • LI XIJIN

Assignees

  • 钰臻元(天津)医疗器械有限公司

Dates

Publication Date
20260512
Application Date
20251224

Claims (13)

  1. 1. A stereoscopic fluorescent tomography apparatus (10), comprising: an imaging unit (1) for imaging an imaging subject (S) to obtain a fluorescence image and an optical coherence tomography image; An image processing part for processing the image shot by the shooting part (1) to obtain a three-dimensional fluorescence tomography image; A plurality of excitation light emitting sections (21) for emitting excitation light beams (211) having a field angle to the subject (S) to excite fluorescence in the subject (S); a tomographic light emission section (22) for emitting a tomographic light beam (221) to perform optical coherence tomography on the photographic subject (S); a first mirror (71) for reflecting the tomographic light beam (221) onto a main optical axis (11) of the photographing section (1); A beam splitter (72) disposed on the main optical axis (11) and configured to split a tomographic light beam (221) reflected by the first mirror (71) into a first light beam (222) and a transmitted second light beam (223) that are directed toward the subject (S) along the main optical axis (11); -a second mirror (73) reflecting said second light beam (223) back to said beam splitter (72); A multi-wavelength light source unit (3) connected to the plurality of excitation light emitting units (21) and the tomographic light emitting unit (22) for providing the plurality of excitation light emitting units (21) with excitation light sources of different wavelengths, respectively, so that the plurality of excitation light emitting units (21) sequentially emit excitation light beams (211) of different wavelengths, respectively, within a predetermined period of time, to sequentially excite fluorescence at a plurality of different depths of the subject (S), respectively, and simultaneously providing the tomographic light emitting unit (22) with tomographic light sources for forming the tomographic light beams (221), and A wavelength-tunable filter (4) disposed in front of the field of view of the imaging unit (1) and configured to filter light emitted to the imaging unit (1) based on the excitation light sources and the tomographic light sources of different wavelengths so that the imaging unit (1) simultaneously obtains fluorescent images of different depths corresponding to the first fluorescent developer and the second fluorescent developer for the imaging subject (S) and an optical coherence tomographic image of the entire imaging subject (S), The image processing part extracts a first separation image corresponding to a first fluorescent developer and a second separation image corresponding to a second fluorescent developer from each fluorescent image, calculates the depth corresponding to each first separation image and each second separation image according to the depth information in the optical coherence tomography, and combines each first separation image and each second separation image according to the depth so as to form a three-dimensional fluorescent tomography corresponding to the first fluorescent developer and a three-dimensional fluorescent tomography corresponding to the second fluorescent developer of the shooting object (S).
  2. 2. The stereoscopic fluorescent tomography apparatus (10) of claim 1, wherein, The image processing unit stacks the first and second separate images according to the depths thereof, and forms a first filling image corresponding to a depth between and/or outside the depths corresponding to the first separate images and a second filling image corresponding to a depth between and/or outside the depths corresponding to the second separate images by interpolation according to the first and second separate images, thereby forming a stereoscopic fluorescent tomographic image corresponding to the first fluorescent developer and a stereoscopic fluorescent tomographic image corresponding to the second fluorescent developer of the whole subject (S).
  3. 3. The stereoscopic fluorescent tomography apparatus (10) of claim 2, wherein, The image processing unit associates the first separated images and the first padded images with the first separated images and/or the first padded images, which have the same depth.
  4. 4. The stereoscopic fluorescent tomography apparatus (10) of claim 1, wherein, Continuously forming the stereoscopic fluorescent layer image.
  5. 5. The stereoscopic fluorescent tomography apparatus (10) of claim 1, wherein, The plurality of excitation light emitting units (21) are annularly arranged on an arc having a first diameter (D1) centered on a main optical axis (11) of the imaging unit (1), and emit the excitation light beam (211) toward the imaging subject (S) so as to be inclined toward the main optical axis (11).
  6. 6. The stereoscopic fluorescent tomography apparatus (10) of claim 1, further comprising: a distance measuring section (5) arranged on the plane of the circular arc for measuring the distance (D2) between the plurality of excitation light emitting sections (21) and the imaging object (S), and A diameter adjustment part (6) which is combined with the plurality of excitation light emitting parts (21) to adjust the first diameter (D1) according to the separation distance (D2) so that the fields of view of the plurality of excitation light beams (211) form an overlapping region (212) which entirely covers the shooting object (S) at the separation distance (D2).
  7. 7. The stereoscopic fluorescent tomography apparatus (10) of claim 6, wherein, The diameter adjustment section (6) adjusts the first diameter (D1) according to the separation distance (D2) so that the fields of view of the plurality of excitation light beams (211) form a maximum overlap region (214) at the separation distance (D2).
  8. 8. The stereoscopic fluorescent tomography apparatus (10) of claim 6, wherein, Further comprises a distance adjusting part, wherein the distance adjusting part is provided with a plurality of distance adjusting parts, The distance adjustment unit is configured to adjust the distance (D2) such that the diameter adjustment unit (6) adjusts the first diameter (D1) according to the adjusted distance (D2) so that the fields of view of the plurality of excitation light beams (211) form an overlapping region (212) that entirely covers the subject (S) at the distance (D2).
  9. 9. The stereoscopic fluorescent tomography apparatus (10) of claim 8, wherein, The distance adjustment unit adjusts the distance D2 according to the size of the subject S, and the diameter adjustment unit 6 adjusts the first diameter D1 according to the adjusted distance D2 so that the fields of view of the plurality of excitation light beams 211 form a maximum overlapping region 214 at the distance D2, the maximum overlapping region being identical to the size of the subject S.
  10. 10. The stereoscopic fluorescent tomography apparatus (10) of claim 6, wherein, Further comprising a plurality of first angle adjusting parts (81), The plurality of first angle adjusting parts (81) are provided on the diameter adjusting part (6) and are combined with the plurality of excitation light emitting parts (21) for adjusting angles by which the plurality of excitation light emitting parts (21) are inclined to the main optical axis (11) side, respectively, to adjust the overlapping region (212).
  11. 11. The stereoscopic fluorescent tomography apparatus (10) of claim 1, wherein, The display unit receives and displays the stereoscopic fluorescent tomogram in real time.
  12. 12. The stereoscopic fluorescent tomography apparatus (10) of claim 6, further comprising: a second angle adjusting part (82) provided on the diameter adjusting part (6) and combined with the tomographic light emitting part (22) for adjusting the angle of the tomographic light emitting part (22), and/or And a third angle adjustment unit (83) that is coupled to the first mirror (71) and adjusts the angle of the first mirror (71).
  13. 13. The stereoscopic fluorescent tomography apparatus (10) of claim 12, wherein, The chromatographic light emitting part (22) is arranged on the circular arc.

Description

Three-dimensional fluorescence tomography device Technical Field The present invention relates to a three-dimensional fluorescence tomography apparatus, and more particularly, to a three-dimensional fluorescence tomography apparatus capable of simultaneously obtaining a fluorescence image and an optical coherence tomography image for the same photographic subject to form a three-dimensional fluorescence tomography image. Background With the development of medical technology, the medical imaging technologies such as fluorescence imaging, optical coherence tomography, CT, nuclear magnetic resonance, B-ultrasonic and the like are endlessly layered. Different medical imaging technologies have respective advantages and pertinence, so in order to fully understand the condition of a patient, doctors generally perform comprehensive examination on affected parts of the patient by adopting various medical imaging technologies so as to comprehensively judge the condition of the patient, thereby obtaining an accurate diagnosis result. Wherein the fluorescent imaging clearly shows tissues, organs, lesions, etc. which are not visible by other medical imaging techniques by means of autofluorescent substances contained in the human body or a previously injected fluorescent developer. Generally, an image obtained through fluorescence imaging is a planar image, and if a stereoscopic fluorescence image is to be obtained, a sample needs to be scanned layer by layer, so that the operation is complicated, the time consumption is long, and the imaging cannot be completed rapidly or in real time. Therefore, there is a strong need for a device that can obtain stereoscopic fluorescent tomograms in real time. Disclosure of Invention Technical problem An object of the present invention is to provide a stereoscopic fluorescent tomography apparatus capable of automatically adjusting the fields of view of a plurality of excitation light beams according to a distance from an object to be photographed so that the fields of view of the plurality of excitation light beams overlap on the object to be photographed and entirely cover the object to be photographed. An object of the present invention is to provide a stereoscopic fluorescent tomography apparatus capable of simultaneously obtaining a fluorescent image and an optical coherence tomography image and combining the fluorescent image into a stereoscopic fluorescent tomography image based on depth information in the optical coherence tomography image. An object of the present invention is to provide a three-dimensional fluorescence tomography apparatus capable of simultaneously obtaining a fluorescence image and an optical coherence tomography image, and combining the fluorescence images corresponding to different kinds of autofluorescent substances and/or fluorescent developers into a three-dimensional fluorescence tomography image based on depth information in the optical coherence tomography image. The technical problems to be solved by the present invention are not limited to the above-described technical problems, and other technical problems to be solved by the present invention can be understood by those skilled in the art from the description of the present invention. Technical proposal A three-dimensional fluorescence tomography apparatus includes an imaging unit configured to capture an object to be imaged to obtain a fluorescence image and an optical coherence tomography image, an image processing unit configured to process the image captured by the imaging unit to obtain a three-dimensional fluorescence tomography image, a plurality of excitation light emitting units annularly arranged on an arc having a first diameter centered on a main optical axis of the imaging unit and configured to emit an excitation light beam having a view angle toward the object to excite fluorescence at the object to be imaged so as to incline toward one side of the main optical axis, a multi-wavelength light source unit connected to the plurality of excitation light emitting units to provide the excitation light source for forming the excitation light beam to the plurality of excitation light emitting units, a distance measuring unit configured to measure a distance between the plurality of excitation light emitting units and the object to be imaged, and a diameter adjusting unit coupled to the plurality of excitation light emitting units to adjust the first diameter according to the distance so that the plurality of excitation light beams overlap the entire view distance at the region of the object to be imaged. As one embodiment, the diameter adjustment portion may adjust the first diameter according to the separation distance so that the fields of view of the plurality of excitation light beams form a maximum overlapping region at the separation distance. As one embodiment, the imaging apparatus may further include a distance adjusting section for adjusting the separation distance such that the d