CN-122004879-A - Flexible PEDOT (polyether-ether-ketone) -PSS (polyphenylene sulfide) hydrogel fiber nerve electrode array and preparation method and application thereof

CN122004879ACN 122004879 ACN122004879 ACN 122004879ACN-122004879-A

Abstract

The invention discloses a flexible PEDOT (polyether-ether-ketone) PSS (polyphenylene sulfide) hydrogel fiber nerve electrode array and a preparation method and application thereof. The preparation method comprises the steps of preparing PEDOT (polyether-ether-ketone) PSS (hydrogel) hydrogel conductive fibers serving as electrodes by adopting a wet spinning technology, and performing insulation treatment and assembly on the PEDOT (polyether-ether-ketone) PSS hydrogel conductive fibers by adopting uracil grafted polyvinyl alcohol to prepare the flexible PEDOT (polyether-ether-ketone) PSS hydrogel fiber nerve electrode array. The hydrogel fiber nerve electrode array has tissue-level modulus, submicron adjustable geometric dimension, ion-rich water characteristic and open three-dimensional topological structure matched with neurons, can effectively form a blending symbiotic interface with brain tissues, realizes long-term stable tracking of spike signals resolved by single neurons, and provides a feasible information interaction tool for a new generation of biocompatible brain-computer interface equipment.

Inventors

ZHANG TING
WANG MINGXU
LI LIANHUI
Zhou Cunkai
MA JUN
WANG YONGFENG

Assignees

中国科学院苏州纳米技术与纳米仿生研究所

Dates

Publication Date: 20260512
Application Date: 20260131

Claims (10)

1. A preparation method of a flexible PEDOT (polyether-ether-ketone) -PSS (polyphenylene sulfide) hydrogel fiber nerve electrode array is characterized by comprising the following steps of: Preparing PEDOT which is taken as an electrode by adopting a wet spinning technology, namely PSS hydrogel conductive fibers; And performing insulation treatment and assembly on the PEDOT-PSS hydrogel conductive fibers by uracil grafted polyvinyl alcohol to obtain the flexible PEDOT-PSS hydrogel fiber nerve electrode array.
2. The preparation method of claim 1, wherein the diameter of the PEDOT/PSS hydrogel conductive fiber is 1-20 μm; and/or the PEDOT is 10-50 kPa in Young's modulus and 1-10 -11 nN·m 2 in bending stiffness of the PSS hydrogel conductive fiber; And/or the equilibrium swelling ratio of the PEDOT to the PSS hydrogel conductive fiber is 40-60%.
3. The preparation method of the polymer composite material, which is characterized in that the preparation method specifically comprises the steps of adopting a wet spinning technology, taking PEDOT: PSS solution as spinning solution, spinning by using a double coagulation bath wet spinning system, and carrying out annealing treatment again to obtain the PEDOT: PSS hydrogel conductive fiber.
4. The method of claim 3, wherein the content of PEDOT and PSS in the spinning solution is 0.5-2.0 wt%, and/or the solvent in the spinning solution comprises dimethyl sulfoxide; and/or the double coagulation bath wet spinning system comprises a primary coagulation bath device and a secondary coagulation bath device; And/or the primary coagulation bath adopted by the double coagulation bath wet spinning system comprises isopropanol solution of phosphoric acid, wherein the temperature of the primary coagulation bath is preferably 15-35 ℃, the concentration of the primary coagulation bath is preferably 0.05-0.1 mol/L, the temperature of the secondary coagulation bath is preferably 85-100 ℃, and the volume concentration of the secondary coagulation bath is preferably 85vol%; And/or the secondary coagulation bath adopted by the double coagulation bath wet spinning system comprises an N' N dimethylformamide aqueous solution; And/or when spinning is carried out by adopting a wet spinning technology, the spinning speed of the fiber in the primary coagulation bath is 1.0-10 mu L/min -1 , and the drafting ratio of the fiber in the secondary coagulation bath is 0.5-1.5; And/or the temperature of the primary annealing treatment is 140-160 ℃ and the time is 15-45 min.
5. The preparation method according to claim 1, characterized in that it comprises in particular: 2-amino-4-hydroxy-6-methylpyrimidine and 1, 6-diisocyanate hexane are mixed and reacted at 100 ℃ for 16h to obtain an intermediate product; and mixing the intermediate product with polyvinyl alcohol and N-methyl-2-pyrrolidone, and stirring and reacting for 1-3 hours at the temperature of 75 ℃ in a protective atmosphere to obtain uracil grafted polyvinyl alcohol.
6. The process according to claim 5, wherein the molar ratio of 2-amino-4-hydroxy-6-methylpyrimidine to 1, 6-diisocyanate hexane is 0.7:4.75; and/or the molar ratio of the intermediate product to the polyvinyl alcohol is 0.75-1.25:5.
7. The preparation method according to claim 1, characterized in that it comprises in particular: Dissolving uracil grafted polyvinyl alcohol in water to form a packaging solution; The encapsulation solution is adopted to dip-coat and insulate the PEDOT-PSS hydrogel conductive fiber, and then the insulation fiber electrode is obtained through drying treatment, Arranging the insulating fiber electrodes in an array manner by adopting a custom-made die, melting an insulating layer at the joint of the electrodes and the PCB gold electrode by using trace ion water, and connecting the electrodes by adopting silver paste; carrying out secondary annealing treatment on the PCB connected with the fiber electrode and the die at 150 ℃ for 30min, and adopting silicone rubber coating to carry out complete encapsulation treatment on the connection part of the gold-silver paste and the fiber electrode of the PCB; and the liquid nitrogen brittle fiber electrode is adopted, the tail electrode point is exposed, the mould is removed, and the flexible PEDOT-PSS hydrogel fiber nerve electrode array is prepared.
8. The method of claim 7, wherein the concentration of the encapsulating solution is 10-20wt%; And/or dip-coating speed adopted by the dip-coating insulation treatment is 1-5 mm.s -1 ; and/or the temperature of the drying treatment is 75 ℃.
9. A flexible PEDOT: PSS hydrogel fiber nerve electrode array produced by the production process of any one of claims 1 to 8.
10. The use of the flexible PEDOT: PSS hydrogel fiber neural electrode array according to claim 9 in neuron resolution, electroencephalogram signal monitoring or flexible brain-computer interface.

Description

Flexible PEDOT (polyether-ether-ketone) -PSS (polyphenylene sulfide) hydrogel fiber nerve electrode array and preparation method and application thereof Technical Field The invention belongs to the technical fields of flexible electronics, nerve engineering and biosensing, and particularly relates to a flexible PEDOT (polyether-ether-ketone) PSS (hydrogel fiber) nerve electrode array and a preparation method and application thereof. Background Implantable neural interface technology is a revolutionary tool in brain science research that provides us with a window for sub-millisecond analysis of individual neuronal cell neural activity. Compared to conventional Michigan electrodes and Utah arrays based on silicon and metal, implantable neural electrodes of one-dimensional (1D) fiber structure have good flexibility and micron-scale diameter, they can be implanted into deeper brain regions (compared to 2D electrode devices) and withstand repeated mechanical strain while maintaining stable performance. In addition, the fiber-based flexible nerve electrode allows the combination of optical and electrical stimulation and electrophysiological and biochemical information recording due to the relatively simple manufacturing process, so that the electrode device has great flexibility and application prospect. Furthermore, the efficacy of the neural interface is critical to the degree of matching of the electrode device to the local brain environment, including feature size, mechanical properties, topology, and biochemical characteristics. Mismatch of these properties exacerbates heterogeneity between the nerve electrode and the biological matrix, leading to inflammation, gliosis and even neuronal death. At present, a fiber electrode based on a polymer or carbon material loaded with metal is generally difficult to have mechanical modulus matched with brain tissue and biological safety (toxic ions are released by metal corrosion), so that the long-time, stable and reliable in-vivo use requirement is difficult to meet. In recent years, hydrogel neuroelectronics has received increasing attention for its high similarity and affinity to biological tissues, with beneficial biological properties including high water content, adjustable mechanical modulus, ion-rich environment, and all-organic characteristics. However, current hydrogel nerve electrodes are always limited by limited micromachining techniques and poor charge transfer efficiency, which limits the miniaturization of electrode devices and the rapid detection of individual neuron high frequency action potentials. In addition, the porous hydrogel molecular skeleton lacks strong molecular bonds and crystal structures, so that the porous hydrogel molecular skeleton is easy to be enzymatically degraded and the structure collapses in a physiological environment to cause the functional failure of an electrode device. How to transform the hydrogel concept into a device that can match the neuron geometry and maintain stability and durability remains a pending problem in engineering. In summary, a hydrogel nerve electrode array with micro-nano size, excellent electrical activity and stable physiological environment is developed for single neuron resolution and long-term stable electroencephalogram signal monitoring, and has important research value in the fields of sensors and brain science. Disclosure of Invention The invention mainly aims to provide a flexible PEDOT (polyether-ether-ketone) PSS (hydrogel fiber) neural electrode array as well as a preparation method and application thereof, so as to overcome the defects of the prior art. In order to achieve the purpose of the invention, the technical scheme adopted by the invention comprises the following steps: the embodiment of the invention provides a preparation method of a flexible PEDOT (polyether-ether-ketone) PSS (hydrogel fiber) nerve electrode array, which comprises the following steps: Preparing PEDOT which is taken as an electrode by adopting a wet spinning technology, namely PSS hydrogel conductive fibers; And performing insulation treatment and assembly on the PEDOT-PSS hydrogel conductive fibers by uracil grafted polyvinyl alcohol to obtain the flexible PEDOT-PSS hydrogel fiber nerve electrode array. The embodiment of the invention also provides the flexible PEDOT-PSS hydrogel fiber nerve electrode array prepared by the preparation method. The embodiment of the invention also provides application of the flexible PEDOT-PSS hydrogel fiber nerve electrode array in neuron resolution, electroencephalogram signal monitoring or flexible brain-computer interfaces. Compared with the prior art, the invention has the beneficial effects that: (1) Compared with the current commercial metal microfilaments, PI film nerve electrodes and carbon fiber electrodes, the hydrogel fiber nerve electrode array provided by the invention has tissue-level bending rigidity matched with neurons, submicron-level adjustable geometric dimens