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CN-122005145-A - Operation table for perfusion operation of mice

CN122005145ACN 122005145 ACN122005145 ACN 122005145ACN-122005145-A

Abstract

The invention discloses an operation table for perfusion operation of mice, which comprises a table body, wherein an operation groove is formed in the upper end of the table body, a drain hole is formed in the bottom of the operation groove, a waste liquid collecting assembly is arranged on the table body, the lower end of the drain hole is communicated with the waste liquid collecting assembly, two pairs of limb fixing assemblies are symmetrically arranged on the left side and the right side of the upper end of the table body, a support frame is arranged at the rear end of the upper end of the table body, an anesthetic mask is detachably arranged on the support frame, a hose is communicated with the anesthetic mask, an anesthetic gas bottle is communicated with the other end of the hose, an exhaust assembly is arranged above the table body, liquid pumping systems are arranged on the left side and the right side of the table body, a liquid outlet pipe is communicated with the liquid outlet system, and an injection needle body is arranged at one end of the liquid outlet pipe, which is far away from the liquid pumping system. According to the perfusion device, the platform body, the waste liquid collecting assembly, the limb fixing assembly, the supporting frame, the anesthesia mask, the air draft assembly, the liquid suction system and the injection needle body are matched for use, so that the overall quality and efficiency of a perfusion experiment can be improved.

Inventors

  • OUYANG JIE
  • Si Yongyu
  • NIU QIN
  • CHEN YING
  • ZHOU CHEN

Assignees

  • 昆明医科大学第二附属医院(云南省泌尿专科医院、云南省肝胆胰外科医院)

Dates

Publication Date
20260512
Application Date
20260409

Claims (9)

  1. 1. The utility model provides a mouse perfusion operation is with operation platform, includes stage body (1), its characterized in that operation groove (11) has been seted up to the upper end of stage body (1), wash port (12) have been seted up to the bottom of operation groove (11), be equipped with waste liquid collection subassembly (2) on stage body (1), the lower extreme of wash port (12) communicate in waste liquid collection subassembly (2), the left and right sides symmetry of stage body (1) upper end is provided with two pairs of limbs fixed subassembly (3), the rear end of stage body (1) upper end is equipped with support frame (4), demountable installation has anesthesia face guard (5) on support frame (4), the intercommunication has hose (51) on anesthesia face guard (5), the other end intercommunication of hose (51) has anesthesia gas cylinder (52), the top of stage body (1) is equipped with exhaust component (6), the left and right sides of stage body (1) all is equipped with pumping liquid system (7), pumping liquid system (7) communicate and has drain pipe (8), pumping liquid pipe (8) are equipped with the rear end face plate (81) of stage body (9), injection side (9) are kept away from to be equipped with one end of injection needle assembly (81) The air draft assembly (6) and the liquid suction system (7) are electrically connected with the control panel (9).
  2. 2. The operation table for the mouse perfusion operation according to claim 1, wherein the bottom of the operation groove (11) is in a shape with high left and right sides and low middle, the water drain hole (12) is formed in the middle of the operation groove (11), the front end of the table body (1) is provided with the mounting groove (13), the waste liquid collecting assembly (2) comprises a waste liquid collecting box (21), a waste liquid pipe (22), an electromagnetic valve (23), a telescopic motor (24) and a supporting plate (25), the upper end of the waste liquid pipe (22) is communicated with the electromagnetic valve (23), the upper end of the electromagnetic valve (23) is communicated with the water drain hole (12), the waste liquid collecting box (21) is arranged in the mounting groove (13), the upper end of the waste liquid collecting box (21) is provided with a through hole (26) corresponding to the waste liquid pipe (22), the telescopic motor (24) is vertically embedded in the bottom of the mounting groove (13), the supporting plate (25) is arranged at the upper end of the telescopic motor (24), the upper end of the telescopic motor (21) is communicated with the electromagnetic valve (23), and the waste liquid collecting box (26) is pushed by the supporting plate (25) to move upwards, and the waste liquid collecting box (26) is pushed by the supporting plate (24).
  3. 3. The operation table for a perfusion operation of a mouse according to claim 2, wherein the front side of the waste liquid collection box (21) is provided with a hidden handle (27).
  4. 4. The surgical table for mouse perfusion operation according to claim 3, wherein two first mounting holes (14) are respectively formed in the left side and the right side of the upper end of the table body (1), the limb fixing component (3) comprises a first vertical rod (31), a first limiting rod (32), a first moving rod (33), a first gasket (34), a first fixing knob (35), a first locking knob (36), a first connecting ring (37), a rotating block (38), a fixing needle (39) and a limiting block (310), the first vertical rod (31) is inserted into the first mounting hole (14), the first vertical rod (31) is in sliding fit with the first mounting hole (14), the first fixing knob (35) is arranged on the table body (1), the first fixing knob (35) is in matched use with the first vertical rod (31), the first limiting rod (32) is arranged at the upper end of the first vertical rod (31), the first moving rod (33) is vertically provided with a first sliding chute (311) which is vertically arranged along the length direction, the first sliding rod (311) is inserted into the first sliding chute (311), the utility model discloses a fixed needle, including stopper (310) and fixed needle, first gasket (34) and first locking knob (36) are located in proper order on first gag lever post (32), first locking knob (36) threaded connection in first gag lever post (32), first go-between (37) are located first removal strip (33) are close to one end downside in operating groove (11), the lower extreme of rotating block (38) pass first spout (311) and threaded connection in first go-between (37), the upper end of fixed needle (39) connect in the lower extreme of rotating block (38), the lower extreme of fixed needle (39) is pointed and is used for the limbs of puncture animal and inserts to be located stopper (310).
  5. 5. The mouse perfusion operation table according to claim 4, wherein the rear end of the upper end of the table body (1) is provided with a second mounting hole (15), the support frame (4) comprises a second vertical rod (41), a second limiting rod (42), a second moving bar (43), a second gasket (44), a second fixing knob (45), a second locking knob (46) and a second connecting ring (47), the second vertical rod (41) is inserted into the second mounting hole (15), the second vertical rod (41) is in sliding fit with the second mounting hole (15), the second fixing knob (45) is arranged on the table body (1), the second fixing knob (45) is in cooperation with the second vertical rod (41), the second limiting rod (42) is arranged at the upper end of the second vertical rod (41), a second sliding groove (48) vertically penetrating through the second moving bar (43) is formed in the length direction, the second limiting rod (42) is inserted into the second sliding groove (48) and the second limiting rod (42) is in turn in sliding fit with the second gasket (42), the second locking knob (46) is in threaded connection with the second limiting rod (42), the second connecting ring (47) is arranged on the lower side of one end, close to the operating groove (11), of the second moving bar (43), the hose (51) is detachably communicated with the upper end of the second connecting ring (47), and the anesthetic mask (5) is detachably communicated with the lower end of the second connecting ring (47).
  6. 6. The operation table for mouse perfusion operation according to claim 5, wherein two third mounting holes (16) which are bilaterally symmetrical are formed in the rear end of the upper end of the table body (1), the air draft assembly (6) comprises a support tube (61), a third fixing knob (62), a transparent cover body (63), a fan (64) and an exhaust pipe (65), one end of the support tube (61) is vertically inserted into the third mounting hole (16), the support tube (61) is in sliding fit with the third mounting hole (16), the third fixing knob (62) is arranged on the table body (1), the third fixing knob is fixedly screwed on the support tube (61) to be matched with use, the transparent cover body (63) is covered above the operation table, the upper end of the transparent cover body (63) is communicated with the support tube (61), the lower end of the third mounting hole (64) is communicated with the fan (64), one end of the exhaust pipe (65) is communicated with the other end of the fan (65), and the other end of the exhaust pipe (65) is communicated with the outside.
  7. 7. The surgical table for perfusion operation of mice according to claim 6, wherein the liquid suction system (7) comprises a peristaltic pump (71), a liquid storage bottle (72), a water suction pipe (73) and a one-way valve (74), the peristaltic pump (71) is arranged in the table body (1), the water suction pipe (73) is arranged on the peristaltic pump (71), one end of the water suction pipe (73) is communicated with the liquid storage bottle (72), the other end of the water suction pipe (73) is communicated with the one-way valve (74), and the one-way valve (74) is detachably communicated with the liquid outlet pipe (8).
  8. 8. The operation table for a perfusion operation of a mouse according to claim 7, wherein a holding part (82) is arranged between the injection needle body (81) and the liquid outlet pipe (8), and the holding part (82) is a plate body facing left and right.
  9. 9. The surgical table for a mouse perfusion operation according to claim 8, wherein a fixing groove (17) is formed in the upper end of the table body (1), one end of the hand-held portion (82) is inserted into the fixing groove (17), and when the hand-held portion (82) is inserted into and fixed to the fixing groove (17), the tip of the injection needle extends into the operation groove (11).

Description

Operation table for perfusion operation of mice Technical Field The invention relates to the technical field of experimental equipment, in particular to an operation table for perfusion operation of mice. Background In the fields of neuroscience, histopathology, pharmacology, etc., fixation via cardiac perfusion is one of the key steps in obtaining high quality tissue samples. The technology sequentially fills physiological saline and fixing solution into a circulatory system of an experimental animal (such as a mouse) to remove blood and realize rapid and uniform fixation of tissues, thereby providing a good foundation for subsequent experiments such as immunohistochemistry, in-situ hybridization, electron microscope observation and the like. Currently, most laboratories commonly use homemade simple perfusion devices for performing heart perfusion in mice. Such devices are typically temporarily assembled from foam sheets, common surgical instruments, scalp needles, syringes, infusion lines, and other common consumables. Although the cost is low and the materials are available, the method has a plurality of technical defects in actual operation, and seriously affects the experimental efficiency and the consistency of results. Firstly, the existing self-made device lacks a special animal fixing structure, the body position of a mouse is difficult to stabilize and is easy to shift in the perfusion process, so that puncture failure or perfusion path deviation is caused, secondly, the switching of perfusion liquid (such as normal saline and fixing liquid) depends on manual operation, the operation is complicated, the switching time and the flow rate are difficult to accurately control, uneven tissue fixation or damage is easy to occur, furthermore, the perfusion speed is unstable due to the lack of a constant pressure or constant flow control mechanism, the perfusion effect is influenced, and the repeatability is poor especially when a large number of samples are processed. In addition, the existing device is not generally provided with a waste liquid collecting system, waste liquid generated in the perfusion process is directly dripped on an experiment table, so that the environment is polluted, the biosafety risk exists, and meanwhile, the pungent smell released by the volatile fixing liquid cannot be effectively discharged, so that potential threat is formed to the health of experiment staff. More seriously, in the process of manually replacing liquid or adjusting the flow rate, bubbles are easily introduced into a circulatory system to cause vascular embolism, so that the tissue fixation quality is further reduced. In summary, the current mouse perfusion operation mode based on self-made tools has difficulty in meeting the requirements of modern biomedical research on standardization, safety, repeatability and operation convenience. Therefore, it is needed to design a special operating table for perfusion of mice with reasonable structure, integrated functions and simple operation, so as to solve the above technical problems and improve the overall quality and efficiency of the perfusion experiment. Disclosure of Invention The invention aims to provide an operation table for mouse perfusion operation, which improves the overall quality and efficiency of a perfusion experiment through the mutual matching of all components. The technical aim of the invention is realized by the following technical scheme: The utility model provides a mouse perfusion operation is with operation platform, includes the stage body, the operation groove has been seted up to the upper end of stage body, the wash port has been seted up to the bottom of operation groove, be equipped with waste liquid collection subassembly on the stage body, the lower extreme of wash port communicate in waste liquid collection subassembly, the left and right sides symmetry of stage body upper end is provided with two pairs of limbs fixed subassembly, the rear end of stage body upper end is equipped with the support frame, demountable installation has the anaesthetic mask on the support frame, the intercommunication has the hose on the anaesthetic mask, the other end intercommunication of hose has the anaesthetic bottle, the top of stage body is equipped with the convulsions subassembly, the left and right sides of stage body all is equipped with the drainage system, the drainage system intercommunication has the drain pipe, the drain pipe is kept away from the one end of drainage system is equipped with the injection needle body, the rear side of stage body is equipped with control panel, waste liquid collection subassembly, convulsions subassembly and drainage system all electrically link to control panel. Through adopting above-mentioned technical scheme, each subassembly mutually support, promote the whole quality and the efficiency of perfusion experiment. The invention is further arranged in such a way that the bottom of the operation groove is i