Search

CN-122005320-A - Centella asiatica extract-containing liposome composition and preparation method, preparation and application thereof

CN122005320ACN 122005320 ACN122005320 ACN 122005320ACN-122005320-A

Abstract

The invention relates to a liposome composition containing centella asiatica extract, and a preparation method, a preparation and application thereof, and belongs to the fields of medical appliances, medical or cosmetic preparations, biological medicine manufacturing technology and preparation manufacturing technology. The liposome composition comprises a supermolecule compound, a liposome bilayer, a nonionic surfactant and a solvent, wherein the liposome bilayer encapsulates the supermolecule compound, the supermolecule compound comprises an active ingredient, a stabilizer and an electrostatic crosslinking agent, the active ingredient comprises centella asiatica extract, the stabilizer comprises escin, the electrostatic crosslinking agent comprises L-arginine, the liposome bilayer is composed of lecithin, the nonionic surfactant comprises oleyl alcohol polyether-20, the solvent comprises polyalcohol and water, and the polyalcohol comprises dipropylene glycol. The liposome composition provided by the invention has excellent stability and excellent skin repair and anti-wrinkle effects.

Inventors

  • LIU HAIYAN
  • CHEN SHIFANG
  • Xing Yujia
  • LI ANZHANG

Assignees

  • 广州青囊生物科技有限公司
  • 谷雨生物科技集团股份有限公司

Dates

Publication Date
20260512
Application Date
20260214

Claims (10)

  1. 1. A liposome composition is characterized by comprising a supermolecular complex, a liposome bilayer, a nonionic surfactant and a solvent; wherein the liposome bilayer encapsulates the supramolecular complex; The supermolecule compound comprises an active ingredient, a stabilizer and an electrostatic crosslinking agent; The active ingredient comprises centella asiatica extract; the centella asiatica extract contains madecassic acid, asiatic acid and asiaticoside; The content of the madecassic acid, the asiatic acid and the asiaticoside in the centella asiatica extract is 85-100 wt% calculated by the total weight of the centella asiatica extract; the stabilizer comprises exendin; the electrostatic crosslinking agent comprises L-arginine; The liposome bilayer is composed of lecithin; the nonionic surfactant comprises oleyl alcohol polyether-20; the solvent includes a polyol and water; The polyol comprises dipropylene glycol; the weight ratio of the supermolecule complex to the liposome bilayer is 1.5:1-3.1:1; The weight ratio of the liposome bilayer to the nonionic surfactant is 2.5:1-5:1; the weight ratio of the active component to the stabilizer is 2:1-2.5:1; the weight ratio of the stabilizer to the electrostatic crosslinking agent is 50:1-84:1; the weight ratio of the polyalcohol to centella asiatica extract in the liposome is 5:1-30:1; The weight ratio of the water to the polyol is 0.5:1-2.3:1.
  2. 2. The liposome composition of claim 1, wherein the weight ratio of madecassic acid, asiatic acid and asiaticoside in the centella asiatica extract is (2.5-3.0): 1.0-1.5): 1.
  3. 3. The liposomal composition of claim 1, wherein the centella extract consists of asiatic acid, madecassic acid and asiaticoside.
  4. 4. A liposome composition according to any of claims 1-3, characterized in that the weight ratio of madecassic acid, asiatic acid and asiaticoside is 2.7:1.2:1.
  5. 5. A method for preparing the liposome composition of any one of claims 1 to 4, comprising the steps of: (1) Dissolving an active ingredient in a polyol, and heating and dissolving to obtain an alcohol phase, wherein the weight ratio of the active ingredient to the polyol in the alcohol phase is 1:2.5-1:3.5; (2) Mixing a stabilizer with water to obtain a water phase, wherein the weight ratio of the stabilizer to the water in the water phase is 1:0.8-1:1.5; (3) Mixing the alcohol phase obtained in the step (1) with the water phase obtained in the step (2), and performing ultrasonic treatment to obtain a supermolecule composition; (4) Mixing an electrostatic crosslinking agent with the supermolecule composition obtained in the step (3) to obtain a supermolecule compound; (5) Mixing nonionic surfactant, lecithin and residual polyalcohol, heating and dissolving to obtain solution A, adding the supermolecular compound obtained in the step (4) into the solution A, mixing to obtain mixed solution 1, adding residual water into the mixed solution 1, stirring, homogenizing, filtering, and collecting filtrate to obtain the liposome composition.
  6. 6. The preparation method according to claim 5, wherein the temperature of the heating dissolution in the step (1) is 60 ℃ to 70 ℃; the heating and dissolving temperature in the step (5) is 70-80 ℃.
  7. 7. A formulation comprising a liposome composition according to any one of claims 1 to 4 or a liposome composition prepared by a method according to any one of claims 5 to 6.
  8. 8. The formulation of claim 7, comprising a medical device or cosmetic.
  9. 9. Use of a liposome composition according to any one of claims 1 to 4 or a liposome composition prepared by a preparation method according to any one of claims 5 to 6 in the preparation of a preparation for skin soothing or anti-aging.
  10. 10. The use of claim 9, wherein the formulation comprises a medical device or a cosmetic.

Description

Centella asiatica extract-containing liposome composition and preparation method, preparation and application thereof Technical Field The invention relates to the field of medical appliances, the field of medical or cosmetic preparations, the technical field of biological medicine manufacturing and the technical field of preparation manufacturing, in particular to a centella asiatica extract-containing liposome composition, and a preparation method, a preparation and application thereof. Background Skin is the most important barrier of the human body and its health state is directly dependent on the integrity and functionality of the stratum corneum. In recent years, with the increase of environmental pressure and the change of skin care habits, the damage of skin barrier has become a common problem, and the skin appeal of dryness, sensitivity, aging and the like caused thereby continues to increase. Therefore, the development of functional cosmetic materials capable of effectively repairing skin barriers has become a research hotspot in the industry. Centella asiatica (academic name: CENTELLA ASIATICA (L.) Urban) is also known as herba Erigerontis, herba Saururi, herba Lespedezae Cuneatae, etc., and is a perennial herb of Centella (Centella) of Umbelliferae (Apiaceae). Centella asiatica is taken as a medicinal plant with long application history, and the core functional components of centella asiatica, madecassic acid, asiaticoside and other pentacyclic triterpene compounds are proved to have excellent anti-inflammatory and fibroblast proliferation and collagen synthesis promoting capabilities by modern researches, and have great potential in repairing skin injury and enhancing skin toughness. However, the inherent strong hydrophobicity and relatively high molecular weight of these ingredients makes it difficult to effectively penetrate the stratum corneum of the skin, limiting its bioavailability and full play of the final skin care effect. In order to improve the transdermal delivery efficiency of active ingredients, nanocarrier technologies such as liposomes, nanoemulsions, etc. have been widely explored. Among them, liposomes are considered as a very promising delivery platform because of their phospholipid structure similar to the lipid bilayer of the skin stratum corneum, with good biocompatibility and potential for promoting penetration. However, how to further improve the loading efficiency, formulation stability, encapsulation stability and appearance stability of the platform for specific active ingredients (such as asiatic acid, madecassic acid and/or asiaticoside) and their efficacy performance in complex formulation systems is still a continuing effort of the skilled person. Particularly, under the trend of pursuing modern skin care products with high efficacy and high tolerance, a composition containing centella asiatica active ingredients, which can efficiently and stably deliver centella asiatica active ingredients and is excellent in stability, is developed, and an innovative technology for synergistic barrier repair is realized, so that the composition has important practical significance. Disclosure of Invention In order to solve the technical problems, the invention provides the following technical scheme. In a first aspect, the present invention provides a liposome composition. A liposome composition comprises a supramolecular complex, a liposome bilayer, a nonionic surfactant and a solvent; wherein the liposome bilayer encapsulates the supramolecular complex; The supermolecule compound comprises an active ingredient, a stabilizer and an electrostatic crosslinking agent; The active ingredient comprises centella asiatica extract; the centella asiatica extract contains madecassic acid, asiatic acid and asiaticoside; The content of the madecassic acid, the asiatic acid and the asiaticoside in the centella asiatica extract is 85-100 wt% calculated by the total weight of the centella asiatica extract; the stabilizer comprises exendin; the electrostatic crosslinking agent comprises L-arginine; The liposome bilayer is composed of lecithin; the nonionic surfactant comprises oleyl alcohol polyether-20; the solvent includes a polyol and water; The polyol comprises dipropylene glycol; the weight ratio of the supermolecule complex to the liposome bilayer is 1.5:1-3.1:1; The weight ratio of the liposome bilayer to the nonionic surfactant is 2.5:1-5:1; the weight ratio of the active component to the stabilizer is 2:1-2.5:1; the weight ratio of the stabilizer to the electrostatic crosslinking agent is 50:1-84:1; the weight ratio of the polyalcohol to centella asiatica extract in the liposome is 5:1-30:1; The weight ratio of the water to the polyol is 0.5:1-2.3:1. In some embodiments, the centella asiatica extract has a weight ratio of madecassic acid, asiatic acid and asiaticoside of (2.5-3.0): 1.0-1.5): 1. In some embodiments, (2.5-3.0) of the weight ratio of madecassic acid, asiatic acid and asiati