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CN-122005322-A - PDRN-silk fibroin composite plant outer vesicle liposome and preparation method and application thereof

CN122005322ACN 122005322 ACN122005322 ACN 122005322ACN-122005322-A

Abstract

The invention belongs to the technical field of cosmetic liposome, and relates to a PDRN-silk fibroin composite plant outer vesicle liposome, and a preparation method and application thereof. The preparation method of the liposome comprises the steps of preparing plant outer vesicles, mixing an active ingredient with alcohol, adding a water-soluble silk fibroin solution, stirring and shearing to obtain an active ingredient-silk fibroin complex, dissolving hydrogenated phospholipid and cholesterol in an organic solvent, removing the organic solvent by rotary evaporation to obtain a lipid membrane, adding a PDRN solution and water into the lipid membrane, stirring and mixing, adding the plant outer vesicles and the active ingredient-silk fibroin complex, stirring, shearing and emulsifying to obtain a primary emulsion, homogenizing the primary emulsion under high pressure to obtain a liposome suspension, and filtering to obtain the liposome. The liposome prepared by the invention has small particle size, low PDI, high zeta potential absolute value, excellent stability, good heat resistance, excellent transdermal performance, high active ingredient delivery efficiency and excellent skin repairing and anti-aging effects.

Inventors

  • LI JUNXIANG
  • FU ZIWEN
  • LU YI

Assignees

  • 广州优特佳生物科技发展有限公司
  • 禾美生物科技(浙江)有限公司
  • 广东艾万生物科技有限公司

Dates

Publication Date
20260512
Application Date
20260410

Claims (10)

  1. 1. The preparation method of the PDRN-silk fibroin composite plant outer vesicle liposome is characterized by comprising the following steps: (1) Preparing plant outer vesicles; (2) Mixing the active ingredient with alcohol to obtain a mixture, adding a water-soluble silk fibroin solution, stirring and shearing to obtain an active ingredient-silk fibroin compound, wherein the alcohol comprises at least one of ethylene glycol, propylene glycol, glycerol and butanediol; (3) Dissolving hydrogenated phospholipid and cholesterol in an organic solvent, and removing the organic solvent by rotary evaporation to obtain a lipid membrane; (4) Adding a PDRN solution and water into a lipid membrane, stirring and mixing, adding a plant outer vesicle and an active ingredient-silk fibroin complex, stirring, and then shearing and emulsifying to obtain a primary emulsion; (5) Homogenizing the primary emulsion under high pressure to obtain liposome suspension, and filtering to obtain the PDRN-silk fibroin composite plant outer vesicle liposome.
  2. 2. The method according to claim 1, wherein the active ingredient comprises at least one of curcumin, retinol, tocopherol and its derivatives, resveratrol, ascorbyl tetraisopalmitate, hesperetin, quercetin, bisabolol, lutein, medium/high molecular polysaccharides, polypeptides, recombinant proteins, natural vegetable oils and fats, panthenol, phenethyl resorcinol.
  3. 3. The method of claim 1, wherein the method comprises providing plant tissue, washing, cutting, adding buffer solution, homogenizing under shear and high pressure, centrifuging to remove precipitate, and concentrating by ultrafiltration to obtain plant outer vesicle, wherein the plant comprises at least one of microalgae, black algae, and brown algae.
  4. 4. The method of claim 1, wherein the active ingredient comprises curcumin and/or retinol.
  5. 5. The method according to claim 1, wherein the hydrogenated phospholipid comprises at least one of hydrogenated lecithin, hydrogenated soybean phosphatidylcholine, hydrogenated egg yolk phospholipid, vegetable-based hydrogenated lecithin, hydrogenated phosphatidylcholine, and hydrogenated phosphatidylethanolamine.
  6. 6. The method according to claim 1, wherein the organic solvent comprises at least one of ethanol, chloroform, and ethyl acetate.
  7. 7. The method of claim 2, wherein the active ingredient further comprises spirulina extract, carnosine, peptide derivatives, and euglena parvula extract.
  8. 8. The method according to claim 7, wherein the weight ratio of the spirulina extract to the euglena parvula extract is 1:0-1.5.
  9. 9. A PDRN-silk fibroin complex plant exovesicle liposome prepared according to any one of claims 1-8.
  10. 10. Use of a PDRN-silk fibroin complex plant exovesicle liposome according to claim 9 in the preparation of a cosmetic.

Description

PDRN-silk fibroin composite plant outer vesicle liposome and preparation method and application thereof Technical Field The invention belongs to the technical field of cosmetic liposome, and relates to a PDRN-silk fibroin composite plant outer vesicle liposome, and a preparation method and application thereof. Background Polydeoxyribonucleotide (PDRN) has remarkable cell repair, anti-inflammatory and tissue regeneration promoting effects, but is extremely unstable in vitro, is easily degraded by nucleases, and is difficult to penetrate the stratum corneum of the skin due to its large molecular weight and strong hydrophilicity. Although the traditional liposome can encapsulate the PDRN, the traditional liposome has the problems of poor stability, easy leakage, low transdermal efficiency and the like. The plant outer vesicle (PEVs) is a novel nano-carrier discovered in recent years, has natural biocompatibility and low immunogenicity, but has loose self structure, low entrapment rate of hydrophobic active substances and limited protection capability for PDRN when used alone. Silk Fibroin (SF) has good film forming and bioadhesion, but direct mixing tends to cause phase separation. In the prior art, a stable delivery system which can organically combine the efficient repair capability of PDRN, the stable supporting effect of silk fibroin and the natural targeting of the plant outer vesicle and can simultaneously and efficiently encapsulate the active ingredients is lacking. Disclosure of Invention Aiming at the technical problems, the invention provides a PDRN-silk fibroin composite plant outer vesicle liposome, and a preparation method and application thereof. In one aspect, the invention provides a method for preparing a PDRN-silk fibroin composite plant outer vesicle liposome, comprising the following steps: (1) Preparing plant outer vesicles; (2) Mixing the active ingredient with alcohol to obtain a mixture, adding a water-soluble silk fibroin solution, stirring and shearing to obtain an active ingredient-silk fibroin compound, wherein the alcohol comprises at least one of ethylene glycol, propylene glycol, glycerol and butanediol; (3) Dissolving hydrogenated phospholipid and cholesterol in an organic solvent, and removing the organic solvent by rotary evaporation to obtain a lipid membrane; (4) Adding a PDRN solution (a solution containing the PDRN) and water into a lipid membrane, stirring and mixing, then adding a plant outer vesicle and an active ingredient-silk fibroin complex, stirring, and then shearing and emulsifying to obtain a primary emulsion; (5) Homogenizing the primary emulsion under high pressure to obtain liposome suspension, and filtering to obtain the PDRN-silk fibroin composite plant outer vesicle liposome. Preferably, the active ingredient comprises a water-soluble or hydrophobic active ingredient, more preferably a hydrophobic active ingredient. The active ingredients include, but are not limited to, at least one of curcumin, retinol, tocopherol and its derivatives, resveratrol, ascorbyl tetraisopalmitate, hesperetin, quercetin, bisabolol, lutein, medium/high molecular polysaccharides, polypeptides, recombinant proteins, natural vegetable oils and fats, panthenol, phenethyl resorcinol. The active ingredient further optionally comprises curcumin and retinol. Preferably, the method for preparing the plant outer vesicle comprises the steps of taking plant tissues, cleaning, cutting, adding buffer solution for shearing and homogenizing, adding the buffer solution for high-pressure homogenizing, centrifuging to remove sediment, and concentrating by ultrafiltration to obtain the plant outer vesicle, wherein the plant comprises at least one of microalgae, black algae and brown algae. Preferably, the hydrogenated phospholipid comprises at least one of hydrogenated lecithin, hydrogenated soybean phosphatidylcholine, hydrogenated egg yolk phospholipid, vegetable-based hydrogenated lecithin, hydrogenated phosphatidylcholine, and hydrogenated phosphatidylethanolamine. Preferably, the organic solvent includes at least one of ethanol, chloroform, and ethyl acetate. Preferably, the active ingredient further comprises spirulina extract, carnosine, peptide derivatives and euglena parvula extract. Preferably, the weight ratio of spirulina extract to euglena parvula extract is 1:0-1.5. Preferably, the buffer pH is between 6 and 8 when preparing the plant outer vesicles. The weight ratio of the plant tissue to the buffer solution is 1:5-20. Ultrafiltration concentration is a process of obtaining a retentate by ultrafiltration with an ultrafiltration membrane, which is a concentration process, wherein the ultrafiltration membrane used has a molecular weight cut-off of 80kDa to 350kDa. Preferably, in step (2), the weight ratio of active ingredient to alcohol is 1:10-100. The concentration of the water-soluble silk fibroin is 1-3wt%, and the weight ratio of the water-soluble silk fibroin to the active ingre