CN-122005340-A - Preparation method of antibacterial compound, composition containing taxus mairei and application of composition

Abstract

The invention belongs to the technical field of daily necessities and biological medicines, and particularly relates to a preparation method of an antibacterial compound, a taxus chinensis composition containing south China and application thereof. The invention improves the yield of the antibacterial compound by more than 5.6 times through the co-culture technology of Amycolatopsis sp.26-4 and T. pulmonis TP-B0596, and the compound has broad-spectrum antibacterial activity and can be applied to chemical daily necessities as a preservative.

Inventors

• CHEN BINJIANG
• HU GUIJUN
• ZHAO XUYANG
• PAN CHENGQIAN

Assignees

• 浙江章光101有限公司

Dates

Publication Date

20260512

Application Date

20260410

Claims (6)

1. 1. Use of a compound as a preservative in a commodity, wherein the compound is 。
2. 2. The use according to claim 1, wherein the commodity is a cosmetic.
3. 3. Application of compound in preparation of antibacterial drugs, wherein the compound is 。
4. 4. The preparation method of the antibacterial compound is characterized by comprising the following steps: S1, culturing Amycolatopsis sp.26-4 in a seed culture medium to obtain seed liquid 1; s2, culturing the T. pulmonis TP-B0596 in a seed culture medium to obtain seed liquid 2; S3, simultaneously inoculating the seed liquid 1 and the seed liquid 2 into a fermentation medium for fermentation to obtain fermentation liquid; s4, filtering the fermentation liquor, extracting with an organic solvent, concentrating and purifying to obtain an antibacterial compound, wherein the antibacterial compound is 。
5. 5. A composition containing Taxus chinensis var mairei comprises plant extract, surfactant, aromatic, thickener, chelating agent, pH regulator, colorant and antiseptic, wherein the plant extract contains Taxus chinensis branch and leaf extract, and the antiseptic is 。
6. 6. The composition of claim 5, wherein the plant extract is obtained by decocting branches and leaves of Taxus chinensis, cortex Dictamni Radicis, radix astragali, radix Saposhnikoviae, ginseng radix, radix Angelicae sinensis and radix Polygoni Multiflori Preparata.

Description

Preparation method of antibacterial compound, composition containing taxus mairei and application of composition Technical Field The invention belongs to the technical field of daily necessities and biological medicines, and particularly relates to a preparation method of an antibacterial compound, a taxus chinensis composition containing south China and application thereof. Background Cosmetics are a class of consumer products widely used in people's daily lives. With the improvement of society and the improvement of public health consciousness, consumers put higher demands on the safety of cosmetics, and related regulatory standards are becoming stricter. According to the specification of cosmetic safety technical Specification (2015 edition), the cosmetics need to be subjected to microbial limit detection, and mainly comprise five indexes of total colony count, heat-resistant coliform bacteria, staphylococcus aureus, pseudomonas aeruginosa, mould and saccharomycete. Wherein the limit value of the total colony count of the cosmetics for eyes, lips and children is less than or equal to 500 CFU/g (ml), other types of products are less than or equal to 1000 CFU/g (ml), and all pathogenic bacteria cannot be detected. In recent years, taxus chinensis var mairei has been paid attention to the field of daily chemicals due to various bioactive components thereof, and has been used in skin care and washing products. For example, patent CN109200057a discloses the application potential of polysaccharide extracted from taxus fruit in whitening and anti-aging, and patent CN103417430a reports the application value of hair nourishing shampoo containing taxus extract for promoting microcirculation of scalp dermis and hair follicle. However, since cosmetics are rich in moisture and nutrients, are susceptible to microbial contamination, preservatives are often added in the art to ensure product safety over the shelf life. Although traditional preservatives have good results in bacteriostasis, some ingredients raise concerns due to potential irritation or safety issues, forcing the industry to seek safer, more efficient solutions. The development of a novel preservative system with broad-spectrum antimicrobial activity and good safety is of great importance in the technical fields of cosmetics and medicine. Disclosure of Invention In order to overcome the drawbacks of the prior art. The invention provides a composition containing taxus mairei extract, a preparation method and application thereof. The invention improves the yield of the antibacterial compound by more than 5.6 times through the co-culture technology of Amycolatopsis sp.26-4 and T. pulmonis TP-B0596, and the compound has broad-spectrum antibacterial activity and can be applied to cosmetics as a preservative. The invention solves the technical problems through the following technical proposal. The invention provides a preparation method of an antibacterial compound, which comprises the following steps: S1, culturing Amycolatopsis sp.26-4 in a seed culture medium to obtain seed liquid 1; s2, culturing the T. pulmonis TP-B0596 in a seed culture medium to obtain seed liquid 2; S3, simultaneously inoculating the seed liquid 1 and the seed liquid 2 into a fermentation medium for fermentation to obtain fermentation liquid; S4, filtering, extracting with an organic solvent, concentrating and purifying the fermentation broth to obtain the antibacterial compound, wherein the antibacterial compound is 。 In the present invention, in S1 and S2, the culture temperature of the culture is 25 to 35 ℃ (e.g., 28 to 30 ℃ C., further e.g., 28 ℃ C. Or 30 ℃ C.), and the culture time of the culture is 1 to 10 days (e.g., 4 days). In the present invention, in S1 and S2, the stirring speed during the cultivation of the culture is 50-300rpm (for example, 180 rpm). In the present invention, in S1 and S2, the seed medium comprises glucose, malt extract and yeast extract. In the seed culture medium of the present invention, the concentration of glucose is 2.0-8.0 g/L (e.g., 4.0 g/L), the concentration of malt extract is 5.0-15.0 g/L (e.g., 10.0 g/L), and the concentration of yeast extract is 2.0-8.0 g/L (e.g., 4.0 g/L). In the present invention, in S3, the fermentation is carried out at 20 to 40℃C (e.g., 28 ℃) for 1 to 10 days (e.g., 5 days). In the present invention, in S3, the stirring speed of the fermentation medium during fermentation is 50-300rpm (e.g., 180 rpm). In the present invention, in S3, the fermentation medium comprises oatmeal, feSO 4·7H2O、MnCl2·4H2 O and ZnSO 4·7H2 O. In the fermentation medium, the concentration of oatmeal is 10.0-30.0 g/L (e.g. 20.0 g/L), the concentration of FeSO 4·7H2 O is 0.5-3.0mg/L (e.g. 1.0 mg/L), the concentration of MnCl 2·4H2 O is 0.5-3.0mg/L (e.g. 1.0 mg/L), and the concentration of ZnSO 4·7H2 O is 0.5-3.0mg/L (e.g. 1.0 mg/L). The invention provides application of a compound in cosmetics, wherein the compound is。 In the present invention, the d