CN-122005349-A - Composition with basement membrane repairing effect and preparation method and application thereof

Abstract

The invention discloses a composition for repairing a basement membrane and preparation and application thereof, wherein the composition comprises, by mass, 0.01% -0.2% of recombinant IV type collagen, 0.4% -4.0% of yeast fermentation product extract, 0.01% -0.2% of acetyl tetrapeptide-9, 0.01% -0.2% of acetyl tetrapeptide-11, 4% -10% of 1, 2-hexanediol, 0.01% -0.5% of ethylhexyl glycerol and the balance of water. The preparation method has the advantages of greatly improving the activity of skin cells and promoting cell migration, and effectively protecting and promoting the repair of the basement membrane by obviously promoting the components of the basement membrane, enhancing the skin barrier, resisting inflammation, relieving the skin, and the like.

Inventors

• TENG FEI
• LIANG WANLING
• HUANG YIFAN
• YAN MEIHUA

Assignees

• 广州市雅资达化妆品有限公司

Dates

Publication Date

20260512

Application Date

20260204

Claims (6)

1. 1. A composition for repairing a basement membrane is characterized by comprising, by mass, 0.01% -0.2% of recombinant type IV collagen, 0.4% -4.0% of yeast fermentation product extract, 0.01% -0.2% of acetyl tetrapeptide-9, 0.01% -0.2% of acetyl tetrapeptide-11, 4% -10% of 1, 2-hexanediol, 0.01% -0.5% of ethylhexyl glycerol and the balance of water.
2. 2. The composition for repairing basement membrane according to claim 1, wherein the recombinant type IV collagen is prepared from pichia pastoris engineering bacteria GS115-pPIC9K-COL4 A1.
3. 3. The composition for repairing a basement membrane according to claim 1, wherein the yeast fermentation product extract is prepared by fermentation of pichia pastoris.
4. 4. A process for preparing a composition for repairing a basement membrane according to any one of claims 1 to 3, The preparation process is as follows: 1) Weighing yeast fermentation product extract, recombinant type IV collagen, acetyl tetrapeptide-9 and acetyl tetrapeptide-11 according to the requirement, adding into water, stirring to dissolve thoroughly to obtain water phase; 2) Weighing 1, 2-hexanediol and ethylhexyl glycerol, uniformly mixing the 1, 2-hexanediol and the ethylhexyl glycerol to obtain an alcohol phase, then mixing the alcohol phase with the water phase, and fixing the volume to a specified weight by using purified water, and uniformly stirring and mixing; 3) And finally, filtering and sterilizing the solution obtained in the last step to obtain the composition.
5. 5. Use of a composition for repairing a basement membrane according to any one of claims 1 to 3 in the preparation of a cosmetic.
6. 6. The use according to claim 5, wherein the cosmetic is in the form of any of aqueous, emulsion, cream, essence.

Description

Composition with basement membrane repairing effect and preparation method and application thereof Technical Field The invention relates to the technical field of cosmetics, in particular to a cosmetic with a basement membrane repairing effect. Background The skin basement membrane exists between animal epithelial cells and stromal cells, and is an extracellular matrix with a soft and tough omentum structure, wherein the thickness of the extracellular matrix is 50-100 nm. The basal membrane of the skin separates the basal cells of the epidermis from the tissues underlying it, provides structural support to the cells and maintains the function between the epidermis and the dermis through the regulation of the cytokines between the epidermis and the dermis, while also having a certain permeability barrier. The skin basement membrane comprises type IV Collagen (COLIV) and type VII Collagen (COLVII), laminin (LN), nidogen (nidogen)/endotoxel (entactin), and monisin (perlecan). COLIV and laminin are present in the dense layer, both forming a network structure that is critical to the stability of the basement membrane. Laminin, proteoglycan, heparin sulfate, etc. are present in the transparent layer. COLXVII is connected with the semi-bridge particles through the transparent layer and is combined with the compact layer, and COLVII exists in the compact lower layer. While nestin/endodysfunctional and monisin bridge on laminin and COLIV networks, improving the stability of the basement membrane and affecting the structural integrity of the basement membrane. The skin basement membrane takes IV type collagen as a core and is matched with components such as laminin and the like to play a multi-dimensional skin care role. The method comprises the steps of physically blocking external harmful substances through accurate aperture, reducing the loss of dermal moisture and nutrition to form a deep barrier, screening small molecule nutrition to supply epidermis, combining cell receptors to regulate and control the epidermal renewal steady state, enhancing epidermis-dermis connection by means of an anchoring system, preventing skin from being loosened in a layering manner, regulating and controlling the synthesis of dermis collagen to resist aging, releasing signal peptide when damaged, guiding cells to reconstruct basal membranes, regulating and controlling inflammation, accelerating healing and reducing pigment scars. Basal membrane damage is co-initiated by exogenous stimuli with endogenous factors. In exogenous, ultraviolet is the main cause, which activates enzyme to degrade IV type collagen, and mechanical friction, improper minimally invasive, extreme temperature and irritant skin care products can also directly or indirectly destroy the structure. Endogenous is associated with natural aging (decreased collagen synthesis, increased degradation), inflammatory immune abnormalities (accelerated injury of inflammatory factors), metabolic disorders (lack of key nutrients or metabolic disease affecting supply). After injury, sensitive redness, red blood wires, loose fine lines and uneven pigments appear in appearance, and the functional manifestation is dry skin, reduced repair capability and susceptibility to microbial infection caused by barrier deterioration. The integrity of the basal lamina can be protected, and the skin problems such as sensitivity, dryness, aging and the like can be radically improved. Although various cosmetics for skin care are available in the market, the skin is stimulated by the component problem, and the repairing effect on the basement membrane is not obvious. Therefore, if a product which is mild and comfortable and truly has an effective repairing effect on the basement membrane, and can radically improve the associated skin problems, the product has a huge application scene. Disclosure of Invention The invention aims to provide a composition with a basement membrane repairing effect, a preparation method and application thereof, and aims to solve the problems that related products for repairing basement membranes and improving skin states are not mild and effectively in the prior art. In order to achieve the above purpose, the invention adopts the following technical scheme: The composition for repairing the basement membrane comprises, by mass, 0.01% -0.2% of recombinant type IV collagen, 0.4% -4.0% of yeast fermentation product extract, 0.01% -0.2% of acetyl tetrapeptide-9, 0.01% -0.2% of acetyl tetrapeptide-11, 4% -10% of 1, 2-hexanediol, 0.01% -0.5% of ethylhexyl glycerol and the balance of water. Further, the recombinant IV type collagen is prepared from Pichia pastoris engineering bacteria GS115-pPIC9K-COL4A1, wherein the Pichia pastoris engineering bacteria GS115-pPIC9K-COL4A1 is derived from the China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No.30004 and is disclosed in a patent application document of CN 118852407A. Further, t