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CN-122005350-A - Sebum-imitating recombinant collagen antioxidation anti-aging brightening composition and preparation method thereof

CN122005350ACN 122005350 ACN122005350 ACN 122005350ACN-122005350-A

Abstract

The invention discloses an anti-oxidation anti-aging brightening composition imitating sebum recombinant collagen and a preparation method thereof, which are in a core-shell biphasic structure, and comprise an aqueous phase core, an oil phase layer and an oil phase layer, wherein the active component of the aqueous phase core is a covalent bond of carboxymethylated recombinant human type III collagen and eclipta oligopeptides, the oil phase layer wraps the aqueous phase core and comprises an anti-oxidation active substance imitating sebum three-phase membrane and a sustained release carrier dispersed in the three-phase membrane, the volume ratio of the aqueous phase core to the oil phase layer is 1:2 to 1:4. The structure not only ensures the stability of the water phase core through a chemical covalent bond, but also remarkably improves the percutaneous permeability and biocompatibility of the composition by utilizing the high similarity of the imitated sebum membrane and skin lipid.

Inventors

  • Cai Qiaxuan
  • ZHENG BOXIANG
  • LI JUNYU

Assignees

  • 广州玖瑟生物科技有限公司

Dates

Publication Date
20260512
Application Date
20260410

Claims (10)

  1. 1. An antioxidant and anti-aging brightening composition imitating sebum recombinant collagen is characterized by being in a core-shell dual-phase structure, and comprises the following components: the active ingredient of the water phase core is a covalent bond compound of carboxymethylation recombinant human type III collagen and eclipta oligopeptides; an oil phase layer, which wraps the water phase core and comprises a sebum-imitating three-phase film, an antioxidant active substance dispersed therein and a sustained release carrier; Wherein the volume ratio of the water phase core to the oil phase layer is 1:2 to 1:4.
  2. 2. The sebum-like recombinant collagen antioxidant and antiaging lightening composition according to claim 1, wherein the degree of modification of the carboxymethylated recombinant human type III collagen is 0.8 to 1.2mmol/g, and the mass ratio of the carboxymethylated recombinant human type III collagen to the eclipta oligopeptide in the covalent bond is 5:1 to 10:1.
  3. 3. The sebum-imitating recombinant collagen antioxidation and anti-aging brightening composition according to claim 1, wherein the sebum-imitating three-phase membrane is composed of soyasterol, linoleic acid and ceramide 3B according to a weight ratio of 3-4:2-3:1.
  4. 4. The sebum-imitating recombinant collagen antioxidant and antiaging brightening composition according to claim 1, wherein the antioxidant active matter includes samara oil tree seed polyphenol and sea buckthorn seed husk flavone, the slow released carrier includes poly beta-amino ester, and the samara oil tree seed polyphenol content is 0.5-2.0%, sea buckthorn seed husk flavone content is 0.05-0.1% and poly beta-amino ester content is 0.1-0.3% based on the total weight of the oil phase layer.
  5. 5. The sebum-imitating recombinant collagen antioxidation and anti-aging brightening composition according to claim 4, wherein the oil phase layer further comprises aluminum magnesium silicate, the content of which is 0.2-0.4% of the total weight of the oil phase layer, and the poly beta-amino ester is degraded under the environment of pH4.5-5.5, so that the composition has the slow release characteristic of the active ingredient for 8-12 hours.
  6. 6. The sebum-imitating recombinant collagen antioxidation and anti-aging brightening composition according to claim 1, further comprising a moisturizing aid, wherein the moisturizing aid consists of sodium hyaluronate and panthenol, and the mass ratio of the sodium hyaluronate to panthenol is 1-2:1.
  7. 7. A method of preparing a sebum-mimicking recombinant collagen antioxidant and anti-aging lightening composition according to any one of claims 1 to 6, comprising the steps of: (1) Preparing an aqueous phase core, namely performing carboxymethylation modification on the recombinant humanized type III collagen, and performing covalent connection reaction on the recombinant humanized type III collagen and the eclipta oligopeptide to obtain the aqueous phase core; (2) Preparing an oil phase layer, namely melting and mixing sebum-imitating three-phase film components, adding an antioxidant active substance and a sustained-release carrier, and uniformly dispersing to obtain the oil phase layer; (3) And forming a core-shell structure, namely adding the water phase core into the oil phase layer under the stirring condition to form the core-shell dual-phase structure composition.
  8. 8. The method of claim 7, wherein in step (1), the carboxymethylation modification is performed by reacting sodium chloroacetate in a phosphate buffer system having a pH of 7.2 to 7.5 and a temperature of 35 to 40 ℃ for 4 to 6 hours under the catalysis of papain, wherein the amount of papain is 0.5 to 1.0% of the weight of the recombinant human type III collagen.
  9. 9. A method of preparing a sebum-mimicking recombinant collagen antioxidant anti-aging lightening composition according to claim 7, wherein in step (1), the covalent attachment reaction is carried out at a ph of 6.8 to 7.0 and a temperature of 25 to 30 ℃ for 2-3 hours.
  10. 10. The method of claim 7, wherein in step (3), the stirring conditions are at a temperature of 35 to 40 ℃ and a rotation speed of 500 to 800r/min, and the stirring time is 30 to 40 minutes.

Description

Sebum-imitating recombinant collagen antioxidation anti-aging brightening composition and preparation method thereof Technical Field The invention belongs to the technical field of cosmetics and skin care products, and particularly relates to an anti-oxidation and anti-aging brightening composition imitating sebum recombinant collagen and a preparation method thereof. Background With the increasing demands of skin anti-aging and whitening and brightening, the application of bioactive components in the fields of cosmetics and skin care products has become a research and development focus. Recombinant human collagen is regarded as an ideal substitute of traditional animal-derived collagen due to good biocompatibility, low immunogenicity and definite repair function, and is often compounded with various plant extracts (such as polyphenol and flavonoid) so as to realize multiple effects of resisting oxidation, promoting collagen regeneration and the like. In order to improve the stability and transdermal efficiency of active ingredients, delivery system technologies have evolved, for example, embedding with carriers such as liposomes, polymeric microspheres, etc., wherein core-shell structures are of interest for achieving physical isolation and controlled release of the contents. Meanwhile, biomimetic liposomes (such as ceramide-containing, sterol-containing systems) that mimic the natural lipid composition of skin have also been developed to improve the skin affinity and barrier repair function of the formulation. The prior art aims at cooperating different active substances through various physical mixing, encapsulation or simple chemical modification means so as to solve the complex problems of skin aging, pigmentation, oxidative stress and the like. However, the prior art solutions still have a systematic deficiency. Most compositions simply physically mix or simply encapsulate the recombinant collagen with the plant active ingredient, lack a stable chemical linkage, result in the active ingredient being susceptible to dissociative inactivation during storage or delivery, and are difficult to achieve synergistic effects. Common delivery vehicles (such as common liposomes or emulsions) have poor responsiveness to the skin microenvironment, and cannot achieve intelligent slow release of the active ingredient at the site of action, often resulting in rapid release with short duration of action. In addition, the traditional bionic liposome system focuses on moisturizing and barrier repair, has insufficient functional fusion with the core anti-aging active component, and is difficult to synchronously achieve the comprehensive effects of long-acting antioxidant, deep collagen promotion and instant brightening. At the technical level, the modification of collagen by the existing methods is often random and uncontrollable, and it is difficult to precisely introduce specific functional groups to optimize its interactions with other ingredients and the final formulation stability. Therefore, developing an integrated core-shell structure system which can realize stable covalent bonding of active ingredients, has environment-responsive slow release capability and is highly cooperated with the efficacy components becomes a key challenge for improving the comprehensive performance of the product. Disclosure of Invention In order to overcome the defects in the prior art, the invention provides an antioxidant and anti-aging brightening composition imitating sebum recombinant collagen and a preparation method thereof, and solves the problems that stable covalent bonding, intelligent slow release and functional synergy with a bionic carrier of active ingredients are difficult to realize in the prior art. In order to achieve the above purpose, the present invention provides the following technical solutions: An antioxidant anti-aging brightening composition imitating sebum recombinant collagen and a preparation method thereof are provided, which are in a core-shell dual-phase structure, and comprise: the active ingredient of the water phase core is a covalent bond compound of carboxymethylation recombinant human type III collagen and eclipta oligopeptides; an oil phase layer, which wraps the water phase core and comprises a sebum-imitating three-phase film, an antioxidant active substance dispersed therein and a sustained release carrier; Wherein the volume ratio of the water phase core to the oil phase layer is 1:2 to 1:4. Preferably, the modification degree of the carboxymethylated recombinant human type III collagen is 0.8 to 1.2mmol/g, and the mass ratio of the carboxymethylated recombinant human type III collagen to the eclipta oligopeptide in the covalent bond is 5:1 to 10:1. Preferably, the sebum-imitating three-phase film consists of soyasterol, linoleic acid and ceramide 3B in the weight ratio of 3-4:2-3:1. Preferably, the antioxidant active substances comprise samara oil tree seed polyphenol and sea buckthorn seed