CN-122005351-A - Preparation method of supermolecule controlled olease composition and application of supermolecule controlled olease composition in cosmetics

Abstract

The invention relates to the technical field of cosmetics, in particular to a preparation method of a supermolecule controlled oleic acid enzyme composition and application thereof in cosmetics, the preparation method of the supermolecule controlled oleic acid enzyme composition comprises the steps of preparing a supermolecule NaDES solvent, reacting mandelic acid, betaine and octanoylglycine to obtain a supermolecule NaDES solvent, preparing the supermolecule controlled oleic acid enzyme composition by reacting papain and bromelain compounds with a supermolecule NaDES solvent, preparing mandelic acid, betaine and octanoylglycine into a supermolecule deep eutectic solvent, immobilizing the protease compound in the supermolecule deep eutectic solvent, and the supermolecule controlled oleic acid enzyme composition finally obtained has the characteristics of simple operation, good solubility, good stability and the like, and can realize the combination of acid, amino acid derivatives and enzymes, achieve the effects of synergistically removing horniness and blockage of skin, controlling oil, rapidly improving the texture and appearance of skin and promoting metabolism of skin.

Inventors

• Xiao Rongshen
• TANG LIFENG
• LI XING
• LI YAN

Assignees

• 广州稀咖科技有限公司

Dates

Publication Date

20260512

Application Date

20260316

Claims (10)

1. 1. A method for preparing a supermolecule controlled oleate enzyme composition, which is characterized by comprising the following steps: Step A, preparing a supermolecule NaDES solvent, namely reacting mandelic acid, betaine and octanoylglycine to obtain a supermolecule NaDES solvent; And B, preparing a supermolecule acidolase composition, namely reacting a papain and bromelain compound with a supermolecule NaDES solvent to obtain the supermolecule controlled-oleic enzyme composition.
2. 2. The method of preparing a supramolecular controlled-oleic enzyme composition as claimed in claim 1, wherein the molar ratio of mandelic acid, betaine and octanoylglycine in step A is (1-2): 0.1-0.2.
3. 3. The method of preparing a supramolecular controlled-oleic enzyme composition as claimed in claim 1, wherein the reaction conditions in step A are inert atmosphere, heating and stirring at 50-80 ℃ for 2-6 hours, and ultrasonic treatment is carried out at a frequency of 25-45KHz while stirring.
4. 4. The method of preparing a supramolecular controlled olease composition as claimed in claim 1, wherein the supramolecular NaDES solvent obtained in the step A is a uniform yellowish transparent liquid.
5. 5. The method of claim 1, wherein the papain/bromelain complex in the step B is prepared by mixing papain and bromelain in a mass ratio of 1:1.
6. 6. The method of preparing a supramolecular controlled-oleic enzyme composition as claimed in claim 1, wherein the mass ratio of papain and bromelain complex to supramolecular NaDES solvent in step B is (1-3) (97-99).
7. 7. The method of preparing a supramolecular controlled-oleic enzyme composition as claimed in claim 1, wherein the reaction conditions in step B are inert atmosphere, heating at 30-40 ℃ for 1-5 hours.
8. 8. The method of claim 1, wherein the supramolecular controlled-oleic enzyme composition obtained in the step B is a uniform pale yellow transparent liquid.
9. 9. A supramolecular controlled olease composition obtainable by the process of preparing a supramolecular controlled olease composition according to anyone of claims 1-8.
10. 10. A cosmetic use of the supermolecule controlled oleic acid enzyme composition as claimed in claim 9, wherein the supermolecule controlled oleic acid enzyme composition is used for preparing cosmetics for removing waste cutin, removing pore obstruction, controlling oil, improving skin quality or promoting skin metabolism.

Description

Preparation method of supermolecule controlled olease composition and application of supermolecule controlled olease composition in cosmetics Technical Field The invention relates to the technical field of cosmetics, in particular to a preparation method of a supermolecule controlled olease composition and application thereof in cosmetics. Background The human skin cuticle consists of chemical components such as keratin, intercellular lipid, natural moisturizing factors and the like, wherein the keratin accounts for more than 60% of the total weight of the cuticle, is a water-insoluble protein and is mainly divided into alpha-keratin and beta-keratin, and a large number of disulfide bonds, hydrogen bonds and the like exist between the structures to form a stable three-dimensional space structure. The existence of a large amount of disulfide bonds makes the stereochemical structure of the keratin very firm, and has the characteristics of strong chemical reagent resistance and hydrolase resistance, so that the disulfide bonds are broken first, and the protein has no property of resisting decomposition and being insoluble in water. Normal metabolism of the stratum corneum is critical to maintaining skin health. The accumulation of waste cutin can cause pore blockage, dark complexion and even acne. The acid or enzyme can remove old and waste cutin and blocked pores of the skin, quickly improve the texture and appearance of the skin and reduce the occurrence of acne, but the action mechanisms are quite different. The acid exfoliating agent plays a role in low pH (pH < 4.5), so that keratin, intercellular lipid and natural moisturizing factors are rapidly hydrolyzed under acidic conditions, dead skin cells are rapidly shed, and part of small molecular fruit acid or fat-soluble acid can penetrate through the stratum corneum to continuously play a physiological role in deep skin. Whereas proteases, due to their catalytic specificity and relatively high molecular weight, only exert a gentle hydrolytic action on keratin on the skin surface. Proteases have many excellent properties as one of the cosmetic active ingredients, but the vulnerability of enzymes has been a problem in research applications. The free enzyme is unstable, easy to deactivate under the conditions of heat, acid, alkali and organic solvent, and lose catalytic action, and the enzyme is also deactivated after being stored for a period of time in the optimal reaction environment of the enzyme. Due to the harsh storage conditions of the natural product enzymes, most enzymes lose activity when stored at room temperature for a month or even less. In addition, the prior art has the limitation that the component cooperativity is insufficient, and the improvement of the third component on the system performance is not fully considered. For example, the C8 carbon chain of the octanoyl glycine and the polar head of the glycine form a unique amphiphilic structure, so that the percutaneous absorption rate of a water-soluble active substance (such as nicotinamide) can be improved by 2.3 times, and the permeation quantity of an oil-soluble component (such as retinol) can be increased by 1.8 times, thereby realizing high-efficiency transportation across barriers. It is expected to play multiple roles in the system by acting as an additional hydrogen bond donor/acceptor to strengthen the supramolecular network, providing independent skin care efficacy, and possibly improving the interfacial properties of the system to facilitate enzyme contact with the substrate. Therefore, there is a need to develop a novel, multicomponent synergistic supramolecular composition that not only provides stable "shelter" for proteases, but also provides a triple synergy of "acidolysis of cutin + enzymolysis of proteins + oil control of amino acid derivatives" and co-existence with acid components, exerting mild and efficient synergistic exfoliating and skin care effects. Disclosure of Invention In view of the shortcomings of the prior art, one of the purposes of the present invention is to provide a preparation method of a supermolecule controlled oleate enzyme composition, another purpose of the present invention is to provide a supermolecule controlled oleate enzyme composition, and a third purpose of the present invention is to provide an application of the supermolecule controlled oleate enzyme composition in cosmetics. One of the purposes of the invention is achieved by the following technical scheme: A method for preparing a supermolecule controlled oleate enzyme composition, comprising the following steps: Step A, preparing a supermolecule NaDES solvent, namely reacting mandelic acid, betaine and octanoylglycine to obtain a supermolecule NaDES solvent; And B, preparing a supermolecule acidolase composition, namely reacting a papain and bromelain compound with a supermolecule NaDES solvent to obtain the supermolecule controlled-oleic enzyme composition. Preferably, the molar rat