CN-122005377-A - Composition for inhibiting TRPV1 activation as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of cosmetics, and discloses a composition for inhibiting TRPV1 activation, and a preparation method and application thereof. The composition comprises a licorice root extract and licochalcone B, wherein the licorice root extract is prepared by enzymolysis and alcohol extraction of licorice root. The invention combines the licorice root extract and licochalcone B, has synergistic effect and low effective concentration, can effectively inhibit the activation of TRPV1, and is particularly characterized by inhibiting the expression of TRPV1 receptor and inhibiting the calcium ion inflow after the activation of TRPV1, and has remarkable inhibition effect on the TRPV1 activation induced by the stimulating components of capsaicin and phenol compounds, which is superior to the traditional TRPV1 antagonist (AMG 9810).

Inventors

• HUANG JIE
• HU GENHUA
• YUAN YUXI
• HE JINGYU
• CHEN QINGSHENG

Assignees

• 广州环亚化妆品科技股份有限公司

Dates

Publication Date

20260512

Application Date

20260115

Claims (10)

1. 1. The application of a composition in preparing a product for inhibiting TRPV1 activation is characterized in that the composition comprises a licorice root extract and licochalcone B, wherein the licorice root extract is prepared by enzymolysis and alcohol extraction of licorice root.
2. 2. The use according to claim 1, wherein the active concentration of licorice root extract is not less than 0.001% by weight.
3. 3. The use according to claim 1, wherein the licochalcone B has an action concentration of 5 μm or more.
4. 4. The use according to claim 1, wherein the enzymatic hydrolysis is carried out using a complex enzyme comprising cellulase and hemicellulase in a mass ratio of (1-2): 1.
5. 5. The use according to claim 1, wherein the enzymatic hydrolysis has a pH of 5-6.5 and/or the enzymatic hydrolysis has a temperature of 45-55 ℃ and/or the enzymatic hydrolysis has a time of 1-2 hours.
6. 6. The use according to claim 1, wherein the alcohol extraction is carried out with an ethanol solution having a concentration of 70-90% by volume, and/or wherein the temperature of the alcohol extraction is 45-55 ℃, and/or wherein the time of the alcohol extraction is 30-50min.
7. 7. The use according to claim 1, wherein the alcohol extraction process assists in ultrasonic extraction at a frequency of 5-6kHz.
8. 8. The use according to claim 1, wherein said inhibiting TRPV1 activation comprises inhibiting the expression level of TRPV1 and/or inhibiting calcium ion influx after TRPV1 activation.
9. 9. The use according to claim 1, wherein the licorice root extract contains total flavonoids and licochalcone B, wherein the total flavonoids are more than 200mg/g, and the licochalcone B is more than 2mg/g.
10. 10. A product for inhibiting TRPV1 activation, which is characterized by comprising a licorice root extract and licochalcone B, wherein the licorice root extract is prepared by enzymolysis and alcohol extraction of licorice root.

Description

Composition for inhibiting TRPV1 activation as well as preparation method and application thereof Technical Field The invention belongs to the technical field of cosmetics, and particularly relates to a composition for inhibiting TRPV1 activation, and a preparation method and application thereof. Background Sensitive muscles are generally considered to be an intolerant condition to changes in the external environment and/or to the performance of the care product. However, such people often cannot tolerate common skin care products, so that milder and safer ingredients are preferably selected when selecting skin care products, so as to avoid the generation of skin discomfort. The pathogenesis of the sensitive muscle is complicated and is caused by the combined action of multiple factors, which is not completely elucidated at present. However, it is generally considered that the formation of the skin-sensory nerve system is closely related to factors such as abnormal skin-sensory nerve system, impaired barrier function, inflammatory reaction, external stimulus, mental stress, skin microecological disturbance and the like, and the factors are mutually promoted and mutually influenced. Among them, sensitive muscles are particularly closely related to sensory nerve hyperreactivity, and related studies indicate that sensitive muscles often accompany excessive activation of transient potential capsaicin channel-1 (TRPV 1) (reference: kueper T, et al Exp Dermatol 2010). TRPV1 is a calcium ion permeable, non-selective cation channel receptor, one of the primary sensors of pain and fever, while also participating in the conduction of itch. TRPV1 can be activated by various stimuli such as pH, capsaicin, uv light, and thermal injury (> 42 ℃) and causes pain through keratinocyte conduction. After TRPV1 is activated, on one hand, the partial skin can be induced to release neuropeptides (such as substance P) to cause the increase of calcium ion inflow and further to cause neurogenic inflammation, on the other hand, the activation of the TRPV1 can cause mast cells to secrete endothelin 1, the endothelin 1 further activates immune cells to cause inflammatory mediators (such as IL1, IL6 and TNF alpha) to be released, and VEGF is up-regulated to cause vasodilation, so that skin inflammatory reaction is aggravated. Thus, TRPV1 channels can be therapeutic targets for inflammatory response symptoms and pain relief. While the conventional TRPV1 antagonist, AMG9810 (CAS: 545395-94-6), has some TRPV1 inhibitory activity, the effect has not yet been expected. Therefore, the development of more efficient TRPV1 inhibition products has become a core requirement and important direction for innovative upgrades of sensitive muscle skin care products. Disclosure of Invention The present invention aims to solve at least one of the technical problems in the prior art described above. Therefore, the invention aims to provide a composition for inhibiting TRPV1 activation, a preparation method and application thereof, and the composition can effectively inhibit TRPV1 activation, including effective reduction of TRPV1 expression and inhibition of intracellular calcium ion signals, has comprehensive effect and low effective concentration, and has an inhibition effect superior to that of the traditional TRPV1 antagonist (AMG 9810). In a first aspect, the present invention provides an application of a composition in preparing a product for inhibiting TRPV1 activation, the composition comprising licorice root extract and licochalcone B, wherein the licorice root extract is prepared by enzymolysis and alcohol extraction of licorice root. In some embodiments of the invention, the licorice root extract has an action concentration of greater than or equal to 0.001wt%. In some embodiments of the invention, the licorice root extract has an effective concentration of 0.001wt% to 2wt%. The concentration of action may be any point value or any two point range value between 0.001wt% and 2wt%, for example 0.001wt%, 0.01wt%, 0.05wt%, 0.1wt%, 0.2wt%, 1wt%, 2wt%. In some embodiments of the invention, the licochalcone B has an action concentration of 5. Mu.M or more. In some embodiments of the invention, the licochalcone B has an action concentration of 5 μm to 40 μm. The concentration of the action may be any point value or any two point range value between 5. Mu.M and 40. Mu.M, for example, 5. Mu.M, 10. Mu.M, 20. Mu.M and 40. Mu.M. In some embodiments of the invention, the enzymatic hydrolysis employs a complex enzyme of cellulase and hemicellulase mixed in a mass ratio of (1-2): 1. In some embodiments of the invention, the enzymatic hydrolysis has a pH of 5 to 6.5. In some embodiments of the invention, the temperature of the enzymatic hydrolysis is 45-55 ℃. In some embodiments of the invention, the time for the enzymatic hydrolysis is 1-2 hours. In some embodiments of the invention, the alcohol extraction is performed with an ethanol solution having a concentr