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CN-122005385-A - Plant exosome composition for beautifying and resisting aging and preparation method thereof

CN122005385ACN 122005385 ACN122005385 ACN 122005385ACN-122005385-A

Abstract

The invention belongs to the technical field of beauty and skin care, and discloses a plant exosome composition for beauty and anti-aging and a preparation method thereof, wherein the composition is prepared by compounding ginger exosome, ginseng exosome and brown algae exosome according to a mass ratio of 1:1.5-2:4, and the preparation process comprises raw material pretreatment, two-stage ultracentrifugation, ultrafiltration concentration, ion chromatographic membrane purification, mixed sterilization and low-temperature preservation. The invention relates to a plant exosome composition for beautifying and resisting aging and a preparation method thereof, wherein natural plants are used as exosome sources, and triple effects of resisting aging and wrinkle, repairing skin barrier and relieving and resisting inflammation of sensitive muscles are realized through synergistic effect among the plant exosome compositions. Meanwhile, the preparation process is optimized, so that the exosome has high purity and stable activity, and the efficacy shows clear dose dependence, and a safe and efficient brand new technical scheme is provided for the research and development of functional cosmetic skin care products.

Inventors

  • SUN YUQING
  • LIU YU
  • MA YUZE
  • XU HONGMEI
  • SHAO YUWEI
  • ZHAO YANG

Assignees

  • 锦悦祉承(辽宁)生物科技有限公司

Dates

Publication Date
20260512
Application Date
20260210

Claims (8)

  1. 1. A plant exosome composition is characterized in that, the plant exosome composition consists of ginger exosome, ginseng exosome and brown algae exosome, the mass ratio of the ginger exosomes, the ginseng exosomes to the brown algae exosomes is 1:1.5-2:4.
  2. 2. A method of preparing a plant exosome composition according to claim 1 comprising the steps of: 1) Preprocessing, namely cleaning ginger, ginseng and brown algae, squeezing juice, filtering, respectively centrifuging, removing sediment and cell fragments, and filtering the supernatant with a filter membrane; 2) Ultracentrifugation, namely, performing first centrifugation on the supernatant of ginger, ginseng and brown algae pretreated in the step 1), taking the precipitate, re-suspending with PBS buffer to obtain a primary sample, and performing second centrifugation respectively, taking the precipitate, re-suspending with PBS buffer to obtain a final sample; 3) Ultrafiltration concentration, namely respectively adopting hollow fiber columns to carry out ultrafiltration concentration on the final sample, and collecting concentrated solution; 4) Purifying the concentrated solution obtained in the step 3) through an ion chromatographic membrane, and collecting eluent to obtain ginger exosomes, ginseng exosomes and brown algae exosomes respectively; 5) Mixing and preserving, namely mixing ginger exosomes, ginseng exosomes and brown algae exosomes according to the mass ratio of 1:1.5-2:4, sterilizing and filtering, and preserving.
  3. 3. The method according to claim 2, wherein the screen for filtration in step 1) has a size of 80 mesh, wherein the centrifugation is performed at 2-5 ℃ for 8-12min at 1000-1200 Xg, 10-30min at 2500-3000 Xg, and 20-40min at 10000-12000 Xg, and the filter has a size of 0.22 μm.
  4. 4. The method according to claim 2, wherein the first centrifugation is 140000-160000 Xg centrifugation at 3-5 ℃ for 80-100min, and the second centrifugation is 170000-190000 Xg centrifugation at 3-5 ℃ for 40-50min.
  5. 5. The method according to claim 2, wherein the hollow fiber column in step 3) has a molecular weight cut-off of 300kDa, a liquid feed rate of 3 to 5LMM and a concentration factor of 5 to 10.
  6. 6. The method according to claim 2, wherein the ion chromatography membrane in step 4) is made of polypropylene and has a volume of 4mL, the purification comprises combining the concentrated solution with a sample membrane, the loading flow rate is 10-30mL/min, the pre-column pressure is 0.1-0.5MPa, an equilibrium Buffer containing 1.0-1.3M KAC-HAC and 0.02-0.05M NaCl is used, the volume of 20-40 membranes is balanced at a flow rate of 10-30mL/min, then an elution Buffer containing 0.1-0.2M Tris-HCl and 0.5-0.8M NaCl is used, and the eluate is collected.
  7. 7. The method of claim 2, wherein the sterile filtration in step 5) is performed using a 0.22 μm PVDF sterile filter at a storage temperature of-80 ℃.
  8. 8. The use of the plant exosome composition according to claim 1 for the preparation of a cosmetic skin care product, wherein the cosmetic skin care product has anti-aging and anti-wrinkle, skin barrier repair and sensitive muscle soothing and anti-inflammatory effects.

Description

Plant exosome composition for beautifying and resisting aging and preparation method thereof Technical Field The invention belongs to the technical field of beauty and skin care, and particularly relates to a plant exosome composition for beauty and anti-aging and a preparation method thereof. Background Skin aging is determined by both internal and external factors. The intrinsic aging is determined by genes, the activity of fibroblasts in skin is reduced with the age, collagen is reduced by about 1% -3% each year, the elastic fiber structure is damaged, the skin is finally dull and rough, the facial contour is loose, dents, wrinkles are grown, and the like, the extrinsic aging refers to skin aging caused by photoaging, environmental pollution, bad living habits and the like, such as UV penetrates through dermis layers, color spots appear, particulate matters such as PM2.5 adhere to the skin surface to cause inflammatory reaction and oxidative damage, and the collagen synthesis is inhibited by staying up, high-sugar diet and the like to accelerate skin saccharification. The exosome is a nanometer vesicle with the diameter of about 30-150nm which is actively secreted by cells, is a core carrier for information transmission and substance exchange among cells, carries core substances such as nucleic acid, protein, lipid, bioactive factors and the like, can precisely act on target cells, and regulates and controls physiological activities such as growth, repair, metabolism and the like of the cells. In recent years, biotechnology and cosmetic science are developed gradually, and exosomes as emerging components show wide application prospects in the fields of medicine, skin care and regenerative medicine. At present, the number of exosome related products applied to the field of beauty and skin care is small, most of the existing exosome products are prepared by depending on animal stem cell sources, so that the preparation cost is high, the corresponding ethical disputes and safety problems exist, meanwhile, the components of the product are single, the multiple skin care requirements of skin aging resistance, barrier repair, allergy relief and inflammation resistance and the like are difficult to be met, and the problems of complicated preparation flow, time consumption, poor purity and the like exist. Therefore, developing an exosome skin care composition with safe sources, reasonable component compounding, comprehensive efficacy and optimized preparation process becomes a technical problem to be solved in the field. Disclosure of Invention In order to solve the technical problems, the invention provides a plant exosome composition for beautifying and resisting aging and a preparation method thereof, breaks through the limitation of traditional cell therapy, plays a role in a cell-free mode, avoids the high cost and safety problems of relying on animal stem cell preparation, and prepares a beautifying and skin care product with multiple effects of resisting aging, repairing barrier, relieving inflammation and the like and high safety by utilizing the natural activity and skin care value of various plant exosomes. In order to achieve the above purpose, the invention provides a plant exosome composition, which consists of ginger exosome, ginseng exosome and brown algae exosome in a mass ratio of 1:1.5-2:4. Also provided is a method for preparing a plant exosome composition, comprising the steps of: 1) Preprocessing, namely cleaning ginger, ginseng and brown algae, squeezing juice, filtering, respectively centrifuging, removing sediment and cell fragments, and filtering the supernatant with a filter membrane; 2) Ultracentrifugation, namely, performing first centrifugation on the supernatant of ginger, ginseng and brown algae pretreated in the step 1), taking the precipitate, re-suspending with PBS buffer to obtain a primary sample, and performing second centrifugation respectively, taking the precipitate, re-suspending with PBS buffer to obtain a final sample; 3) Ultrafiltration concentration, namely respectively adopting hollow fiber columns to carry out ultrafiltration concentration on the final sample, and collecting concentrated solution; 4) Purifying the concentrated solution obtained in the step 3) through an ion chromatographic membrane, and collecting eluent to obtain ginger exosomes, ginseng exosomes and brown algae exosomes respectively; 5) Mixing and preserving, namely mixing ginger exosomes, ginseng exosomes and brown algae exosomes according to the mass ratio of 1:1.5-2:4, sterilizing and filtering, and preserving. Preferably, the screen mesh used in the step 1) is 80 meshes, the centrifugation is carried out at 2-5 ℃ for 8-12min at 1000-1200 Xg, at 2500-3000 Xg for 10-30min, at 10000-12000 Xg for 20-40min, and the filter membrane is 0.22 μm in size. Preferably, step 2) is performed for a first centrifugation of 140000-160000 Xg for 80-100min at 3-5deg.C and a second centrifugation of 170000-190000