CN-122005386-A - Antibacterial composition, preparation method and application thereof

Abstract

The invention belongs to the technical field of biology, and discloses a bacteriostatic composition, a preparation method and application thereof, wherein the bacteriostatic composition comprises 1-5 parts of hop extract, 1-8 parts of gulfweed polyphenol, 1-5 parts of fucoidin and 90-110 parts of water. The preparation method of the antibacterial composition comprises the following steps of S1, washing and sun-drying hops, crushing the hops, extracting hop dry powder in an alcohol aqueous solution system, centrifuging the hops to obtain hop extracts, S2, washing and sun-drying gulfweed, crushing the gulfweed dry powder, extracting the gulfweed dry powder in a natural eutectic solvent system under ultrasonic conditions, centrifuging the gulfweed dry powder to obtain crude extracts, and post-treating the crude extracts to obtain gulfweed polyphenol, S3, carrying out enzymolysis treatment on fucoidin by fucoidin, extracting the fucoidin to obtain fucoidin solution, and S4, finally mixing all the components to obtain the antibacterial composition. The antibacterial composition has a simple formula, can effectively inhibit the proliferation of acne-related pathogenic bacteria, and is simple in preparation method and process, and convenient for expanding production and quality control.

Inventors

• WANG YING
• CHENG FENGZHI
• ZHANG YANGYANG
• LIU JIARU

Assignees

• 青岛农业大学

Dates

Publication Date

20260512

Application Date

20260211

Claims (10)

1. 1. The antibacterial composition is characterized by comprising, by mass, 1-5 parts of hop extract, 1-8 parts of gulfweed polyphenol, 1-5 parts of fucoidin and 90-110 parts of water.
2. 2. The antibacterial composition according to claim 1, which comprises, by mass, 1-4 parts of a hop extract, 1-5 parts of gulfweed polyphenol, 1-3 parts of fucoidin and 90-110 parts of water.
3. 3. The bacteriostatic composition according to claim 1, wherein the hop extract is prepared by washing and sun-drying fresh hops, pulverizing, placing the dried hops powder in an aqueous alcohol system, extracting under shaking, and centrifuging.
4. 4. The antibacterial composition according to claim 1, wherein the gulfweed polyphenol is prepared by washing, sun-drying, pulverizing, extracting with natural eutectic solvent system, centrifuging to obtain crude extract, and post-treating.
5. 5. The bacteriostatic composition according to claim 1, wherein the fucoidin is prepared by adding fucoidin enzyme into fucoidin solution, placing in water bath for enzymolysis, inactivating enzyme, centrifuging, collecting supernatant, classifying the supernatant into fucoidin solutions with different molecular weight segments by ultrafiltration system, and freeze-drying.
6. 6. A method of preparing a bacteriostatic composition according to claim 1, comprising the steps of: s1, preparing a hop extract, namely cleaning and sun-drying fresh hops, crushing, placing hop dry powder into an alcohol aqueous solution system, extracting under a vibration condition, and centrifuging to obtain the hop extract; S2, preparing gulfweed polyphenol, namely cleaning and sun-drying fresh gulfweed, crushing, placing gulfweed dry powder into a natural eutectic solvent system, extracting under ultrasonic conditions, centrifuging to obtain a crude extract, and then performing post-treatment to obtain gulfweed polyphenol; S3, preparing fucoidin, namely dissolving freeze-dried fucoidin by distilled water, mixing the freeze-dried fucoidin with a fucoidin solution with the mass ratio of 0.2% -2% in an equal volume, placing the mixture in a water bath for enzymolysis for 2h, placing the mixture in a 100 ℃ water bath for enzyme deactivation for 10 min, centrifuging the mixture at the temperature of 4 ℃ and 8000r/min for 10 min, and taking supernatant; S4, mixing the hop extract, gulfweed polyphenol and fucoidin to obtain the antibacterial composition.
7. 7. The method of claim 6, wherein in step S1, the alcohol aqueous solution is 45% -60% ethanol or butanediol, the shaking extraction time is 0.5-1.5h, the extraction temperature is 40-60 ℃, the rotation speed is 120-200 r/min, the centrifugation is 10-15 min under the conditions of 4 ℃ and 8000-10000 r/min, and the hops extract is obtained after freeze drying.
8. 8. The preparation method according to claim 6, wherein in the step S2, the natural eutectic solvent system is a mixture of betaine and glycerol in a molar ratio of 1:1, the water content is 40-60%, the ultrasonic treatment condition is that the ultrasonic time is 15-40 min, the ultrasonic temperature is 40-70 ℃, and the centrifugation condition is that the centrifugation is 10-15 min under the conditions of 4 ℃ and 8000-10000 r/min.
9. 9. Use of a bacteriostatic composition according to claim 1, wherein said pathogenic bacteria comprise propionibacterium acnes, staphylococcus epidermidis and staphylococcus aureus.
10. 10. Use of a bacteriostatic composition according to claim 1, in the preparation of a cosmetic, wherein said cosmetic is a gel, a serum, a mask, a cream, an emulsion or a cleansing gel.

Description

Antibacterial composition, preparation method and application thereof Technical Field The invention relates to the technical field of biology, in particular to a bacteriostatic composition, a preparation method and application thereof. Background Acne (vaccinia) is a common skin disorder mainly caused by excessive proliferation of microorganisms such as propionibacterium acnes, staphylococcus epidermidis and staphylococcus aureus. These bacteria multiply in the pilosebaceous glands, leading to inflammatory reactions, and the formation of acne symptoms such as redness, pustules, etc. At present, most common acne removing products in the market contain antibiotics, salicylic acid, benzoyl peroxide and other components, but the components may have side effects, such as skin dryness, irritation, drug resistance and other problems. The existing acne treatment medicines, such as antibiotics, are easy to cause drug resistance of pathogenic bacteria, have poor antibacterial effect after long-term use, and the use of salicylic acid or benzoyl peroxide and other medicines is irritating to skin, is unfavorable for the health state of skin after long-term use, and is easy to cause sensitive muscles. Therefore, the acne-removing product needs to be improved continuously, and the safe, healthy and effective acne-removing product is still to be developed. Disclosure of Invention Aiming at the problems, the invention provides a bacteriostatic composition, a preparation method and application thereof, wherein the bacteriostatic composition mainly comprises a hop extract, gulfweed polyphenol and fucoidin, can effectively inhibit acne pathogenic bacteria such as propionibacterium acnes, staphylococcus epidermidis and staphylococcus aureus, can be used as active ingredients for preparing bacteriostatic cosmetics or skin care products, and is used for preventing and relieving acne problems. In order to solve the problems, the application provides the following technical scheme: In a first aspect, the application provides a bacteriostatic composition which comprises, by mass, 1-5 parts of a hop extract, 1-8 parts of gulfweed polyphenol, 1-5 parts of fucoidin and 90-110 parts of water. In the antibacterial composition, the hop extract, the gulfweed polyphenol and the fucoidin are three natural active ingredients, and the antibacterial composition is safe to use and has antibacterial, anti-inflammatory and antioxidant effects. Wherein flavonoid compounds and polyphenols in the hop extract can effectively inhibit bacterial growth, gulfweed polyphenol has remarkable antioxidant and anti-inflammatory effects, and fucoidin is an oligosaccharide extracted from brown algae, and has effects of regulating skin microecology and enhancing skin barrier function. The three active ingredients are compounded for use, and the synergistic combination of the three can enhance the antibacterial effect of the composition on acne pathogenic bacteria, is safe for use, has no stimulation to skin, and is suitable for long-term use. Optionally, the antibacterial composition comprises, by mass, 1-4 parts of hop extract, 1-5 parts of gulfweed polyphenol, 1-3 parts of fucoidin and 90-110 parts of water. Preferably, in the antibacterial composition, the mass ratio of the hop extract to the gulfweed polyphenol to the fucoidin is 1:1:2. Preferably, the antibacterial composition comprises the following components in percentage by mass of 1% of hop extract, 1% of gulfweed polyphenol, 2% of fucose and the balance of water. In the process of carrying out a large number of experiments, the applicant has unexpectedly found that the synergistic effect of the hop extract, the gulfweed polyphenol and the fucoidin can improve the inhibition effect on acne-related pathogenic bacteria, can effectively inhibit the growth and proliferation of propionibacterium acnes, staphylococcus epidermidis and staphylococcus aureus, inhibit the deterioration of acne and play a role in preventing acne. Optionally, the preparation method of the hop extract in the antibacterial composition comprises cleaning fresh hop, sun-drying, pulverizing, placing the hop dry powder in an alcohol aqueous solution system, extracting under shaking condition, and centrifuging to obtain the hop extract. Optionally, the preparation method of the gulfweed polyphenol in the antibacterial composition comprises the steps of washing, sun-drying and crushing fresh gulfweed, placing dry gulfweed powder in a natural eutectic solvent system, extracting under ultrasonic conditions, centrifuging to obtain a crude extract, and then performing aftertreatment to obtain the gulfweed polyphenol. Optionally, the preparation method of the fucoidin oligosaccharide in the antibacterial composition comprises the steps of adding fucoidin enzyme into fucoidin solution, placing in a water bath for enzymolysis, carrying out enzyme deactivation, centrifuging, collecting supernatant, grading the supernatant into fucoidin solutions wi