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CN-122005397-A - Preparation method of high-activity gentian anti-inflammatory active component capable of relieving

CN122005397ACN 122005397 ACN122005397 ACN 122005397ACN-122005397-A

Abstract

The invention discloses a preparation method of a high-activity gentian anti-inflammatory active component, which belongs to the technical field of preparation of natural plant extracts and comprises the steps of gentian pretreatment, composite probiotic fermentation, composite enzymolysis, fractional purification and low-temperature refining, wherein the composite probiotic is prepared by compounding lactobacillus plantarum and lactobacillus reuteri, the composite enzyme is prepared by compounding cellulase, pectase and papain, the molecular weight of the prepared active component is below 3kDa, the gentiopicroside retention rate is 95%, and the active component also contains micromolecular peptide and organic acid fermented by the probiotics. The invention adopts a fermentation and enzymolysis combined process to realize deep degradation of gentian root, has good skin permeability of active ingredients, and all substances cooperatively promote the anti-inflammatory effect, and the whole preparation process is free of organic solvents, mild and free of stimulation. The component can be added into cosmetics such as facial masks, essence and the like, the addition amount is 0.2% -5.0%, and the cosmetic is suitable for various skin types and has a broad industrial prospect.

Inventors

  • Guang Siyu
  • WANG SHENGLEI

Assignees

  • 广州精粹堂化妆品有限公司

Dates

Publication Date
20260512
Application Date
20260316

Claims (10)

  1. 1. The preparation method of the high-activity gentian anti-inflammatory active ingredient is characterized by comprising the steps of gentian pretreatment, composite probiotics fermentation, composite enzymolysis, grading purification and low-temperature refining, wherein the molecular weight of the prepared active ingredient is below 3kDa, the composite probiotics are compound bactericides of lactobacillus plantarum and lactobacillus reuteri, the composite enzymes are compound bactericides of cellulase, pectinase and papain, the retention rate of gentiopicroside in the active ingredient is 95%, and the active ingredient contains micromolecular peptides and organic acid active substances which are fermented and metabolized by the probiotics.
  2. 2. The preparation method of the high-activity gentian anti-inflammatory active component for soothing is characterized by comprising the steps of taking fresh gentian, removing impurities, cleaning, cutting into slices with the thickness of 2-4 mm, vacuum drying at 45-55 ℃ until the water content is 8%, crushing, and sieving with a 80-120-mesh sieve to obtain gentian powder for later use.
  3. 3. The preparation method of the high-activity gentian soothing anti-inflammatory active ingredient according to claim 1, wherein the ratio of the bacterial activities of lactobacillus plantarum to lactobacillus reuteri in the composite probiotics is (1.5-2.5): 1, and the bacterial activities of lactobacillus plantarum and lactobacillus reuteri are 100-200 hundred million CFU/g.
  4. 4. The preparation method of the high-activity gentian anti-inflammatory active component capable of soothing according to claim 1, wherein the step of fermenting the compound probiotics is that 8-15 times of purified water is added into gentian powder, the pH is adjusted to 6.0-6.8, 0.8% -2.0% of compound probiotics are added into gentian powder, the gentian powder is fermented for 24-30 hours under anaerobic conditions at 36-40 ℃, then the temperature is reduced to room temperature after sterilization is carried out for 15-25 min at 85-95 ℃, and the gentian fermented product is obtained.
  5. 5. The preparation method of the high-activity gentian soothing anti-inflammatory active ingredient according to claim 1, wherein the mass ratio of cellulase, pectase and papain in the compound enzyme is (1-2): 50 ten thousand U/g cellulase enzyme activity, 30 ten thousand U/g pectase activity and 20 ten thousand U/g papain enzyme activity.
  6. 6. The preparation method of the high-activity gentian anti-inflammatory active component for soothing is characterized in that the step of compound enzymolysis is that 0.5% -1.5% by mass of compound enzyme of gentian powder is added into gentian fermentation product, pH is adjusted to 5.0-6.0, enzymolysis is carried out for 3-5 h at 45-55 ℃, enzyme deactivation is carried out for 15-25 min at 85-95 ℃, and cooling is carried out to room temperature, so that gentian enzymolysis product is obtained.
  7. 7. The preparation method of the high-activity gentian anti-inflammatory active component for soothing is characterized by comprising the steps of carrying out rough filtration on gentian enzymatic hydrolysate through 200-300 mesh filter cloth, removing filter residues to obtain rough filtrate, carrying out cross-flow filtration on the rough filtrate through a 3kDa ultrafiltration membrane, wherein the operation pressure is 0.1-0.3 MPa, the temperature is 25-35 ℃, and collecting permeate to obtain gentian purified solution.
  8. 8. The preparation method of the high-activity gentian anti-inflammatory active component for soothing is characterized in that the low-temperature refining step comprises the steps of concentrating gentian purified solution through a nanofiltration membrane until the solid content is 15% -25% to obtain gentian concentrated solution, vacuum freeze-drying the gentian concentrated solution at-40 to-30 ℃, crushing the dried gentian concentrated solution, and sieving the dried gentian concentrated solution with a 100-200-mesh sieve to obtain high-activity gentian anti-inflammatory active component powder for soothing.
  9. 9. The method for preparing the high-activity gentian anti-inflammatory active component with soothing effect according to claim 8, wherein the nanofiltration membrane has a molecular weight cut-off of 200-400 da, a nanofiltration operation pressure of 0.5-1.0 mpa and a temperature of 25-35 ℃.
  10. 10. The application of the high-activity gentian anti-inflammatory active component for relieving prepared by the method of any one of claims 1-9 in the preparation of cosmetics and skin care products is characterized in that the cosmetics and skin care products are one or more of facial masks, essences, emulsions and creams, and the addition mass fraction of the active component in the cosmetics and skin care products is 0.2% -5.0%.

Description

Preparation method of high-activity gentian anti-inflammatory active component capable of relieving Technical Field The invention relates to the technical field of natural plant extract preparation, in particular to a preparation method of a high-activity gentian soothing anti-inflammatory active ingredient. Background The gentian root is the dry root and rhizome of gentian of gentiaceae, the main active ingredient is gentiopicroside, and the gentiopicroside, gentian polysaccharide, flavonoid and other substances are contained, so that the gentiopicroside has remarkable anti-inflammatory, soothing and antioxidant effects, can inhibit the release of skin inflammatory factors, can alleviate discomfort symptoms such as skin redness, itching, stinging and the like, can repair skin barriers, and is a natural plant raw material with great value in the field of cosmetics. At present, the active ingredients of gentian are mainly extracted by the traditional water extraction method, the alcohol extraction method, the ultrasonic auxiliary extraction method and the like, but the methods have the defects that the extracted active ingredients are large in molecular weight, mostly coarse extracts and poor in skin permeability, the active ingredients are difficult to absorb and utilize by skin, so that the anti-inflammatory effect is poor, the traditional extraction method can only extract the inherent active ingredients in gentian and can not generate new active substances, the function is single, the organic solvent is used in the partial extraction method, the risk of solvent residue exists, the heat-sensitive active ingredients such as gentiopicroside and the like are easy to damage, the retention rate is low, the refining degree of the extracted product is low, the impurity content is high, the skin is easy to cause slight irritation when the extracted product is applied to cosmetics, and the application of the extracted product in the skin care product of sensitive muscles is limited. Along with the increasing demands of consumers on natural, efficient and mild cosmetic raw materials, development of a preparation method capable of improving skin permeability and retention rate of active ingredients of gentian and generating new synergistic active substances becomes an urgent need for industry development. The extraction method combining the probiotics fermentation and enzymolysis technology can degrade macromolecular substances in plant raw materials into small molecules, and meanwhile, the probiotics fermentation can generate metabolites such as small molecular peptides, organic acids, vitamins and the like, and the metabolites and the plant inherent active ingredients cooperate to improve the overall efficacy. However, no report on the application of the combination of the composite probiotic fermentation and the composite enzymolysis to the preparation of the gentian high-activity anti-inflammatory component is available at present, so that a preparation method of the gentian active component based on the composite probiotic fermentation-composite enzymolysis is developed, and the preparation method has important application value. Disclosure of Invention Aiming at the problems of poor skin permeability, low active ingredient retention rate, single function, easiness in existence of solvent residues and the like of the existing gentian active ingredient extraction method, the invention provides the preparation method of the high-activity gentian anti-inflammatory active ingredient, which combines the processes of gentian pretreatment, composite probiotic fermentation, composite enzymolysis, fractional purification and low-temperature refining to prepare the high-activity ingredient with the molecular weight of less than 3kDa, not only retains the inherent active ingredients such as gentiopicroside in gentian, but also enriches the metabolites such as micromolecular peptides, organic acids and the like fermented by the probiotics, and the like, and the synergistic effect of the active substances can greatly improve the anti-inflammatory effect, and the high-activity gentian active ingredient is good in skin permeability, free of solvent residues, mild and free of irritation, and is suitable for various cosmetics and skin care products. In order to achieve the above object, the present invention is realized by the following technical scheme: The preparation method of the high-activity gentian anti-inflammatory active ingredient comprises the steps of gentian pretreatment, composite probiotic fermentation, composite enzymolysis, grading purification and low-temperature refining, wherein the molecular weight of the prepared active ingredient is below 3kDa, the composite probiotic is a compound microbial inoculum of lactobacillus plantarum and lactobacillus reuteri, the composite enzyme is a compound enzymatic inoculum of cellulase, pectase and papain, the retention rate of gentiopicroside in the active ingredient is