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CN-122005400-A - Preparation method of sakura extract with flora regulation and antioxidation effects

CN122005400ACN 122005400 ACN122005400 ACN 122005400ACN-122005400-A

Abstract

The invention discloses a preparation method of a sakura extract with flora regulation and antioxidation effects. The preparation method can efficiently extract the active ingredients in the sakura petals through the design of composite enzymolysis, gradient temperature dynamic leaching, ultrasonic assistance, ascorbic acid and citric acid, retains the flora regulation and antioxidant activity of the sakura petals, has mild process and is suitable for industrial mass production.

Inventors

  • WU WENBIN
  • Pan Meifen

Assignees

  • 广州植诗粹科技有限公司

Dates

Publication Date
20260512
Application Date
20260320

Claims (10)

  1. 1. S1, raw material pretreatment, namely selecting fresh, mildew-free and pest-free cherry petals, cleaning, draining, and then performing vacuum freeze drying, wherein the moisture content of the dried cherry petals is controlled to be 0.5-10wt%; pulverizing dried sakura petals, and sieving with 60-80 mesh sieve to obtain sakura petal powder; adding compound enzyme into the sakura petal powder for enzymolysis, wherein the compound enzyme consists of cellulase, pectase and protease according to the weight ratio of 2:0.8-1.1:0.8-1.1, and performing enzyme deactivation treatment after the enzymolysis is finished to obtain the sakura petal powder; S2, gradient dynamic leaching, namely mixing the enzymatic hydrolysis sakura petal powder with an extraction solvent according to the weight ratio of 1:50-100, wherein the extraction solvent is ethanol water solution with the weight percent of 40-60, ascorbic acid accounting for 0.1-0.3 weight percent and citric acid accounting for 0.05-0.15 weight percent of the ethanol water solution are added into the ethanol water solution, adopting a gradient temperature dynamic leaching mode, firstly leaching for 1-2 hours at 20-30 ℃ and then leaching for 1-2 hours at 40-50 ℃, finally leaching for 0.5-1 hour at 55-60 ℃, adopting ultrasonic assistance in the leaching process, the ultrasonic power is 150-250W, the ultrasonic frequency is 20-40kHz, the stirring speed is 150-200r/min for each leaching time is 30-35min, filtering and collecting filtrate after leaching is finished, S3, purifying the crude extract, namely carrying out centrifugal treatment on the crude extract at the rotating speed of 3000-5000r/min and the centrifugal time of 15-25min, carrying out purification on supernatant fluid through a macroporous resin column, eluting impurities by deionized water, eluting by 50-70wt% ethanol water solution, collecting eluent, ultrafiltering the eluent by an ultrafiltration membrane with a molecular weight cut-off of 1000-5000Da, collecting permeate, concentrating and desolventizing by concentrating under reduced pressure at a concentration temperature of 40-50 ℃ and a vacuum degree of-0.08-0.06 MPa until the solid content of the concentrate is 20-30wt%, and spray-drying the concentrate at an inlet air temperature of 160-180 ℃ and an outlet air temperature of 80-90 ℃ at a feed speed of 5-15mL/min to obtain the sakura petal active extract.
  2. 2. The preparation method of claim 1, wherein in S1, the addition amount of the compound enzyme is 0.5-1.5wt% of the sakura petal powder, the enzymolysis condition is that the enzymolysis temperature is 35-45 ℃, the enzymolysis time is 1-2h, the pH value of an enzymolysis system is 4.5-5.5, and the enzyme deactivation treatment condition is that the temperature is 90-100 ℃ and the time is 10-15min.
  3. 3. The method according to claim 1, wherein in S1, the conditions of vacuum freeze-drying are that the prefreezing temperature is-40 to-30 ℃, the prefreezing time is 2-4 hours, the sublimation drying temperature is-20-0 ℃, the sublimation drying time is 8-12 hours, the resolution drying temperature is 20-30 ℃, and the resolution drying time is 4-6 hours.
  4. 4. The preparation method of claim 1, wherein in S3, the macroporous resin is AB-8 type macroporous adsorption resin, the diameter-to-height ratio of the macroporous resin column is 1:8-12, the loading speed is 1-2BV/h, the eluting speed of deionized water is 2-3BV/h, the eluting amount is 3-5BV, the eluting speed of 50-70wt% ethanol water solution is 1-2BV/h, and the eluting amount is 2-4BV.
  5. 5. The process according to claim 1, wherein the ultrafiltration is carried out at a pressure of 0.1 to 0.3MPa, a temperature of 25 to 35℃and an ultrafiltration time of 30 to 60 minutes in S3.
  6. 6. The process according to any one of claims 1 to 5, wherein in S5, 5 to 10% by weight of dextrin, having a DE value of 10 to 20, is added to the concentrate before spray drying.
  7. 7. A sakura extract having flora-modulating and antioxidant effects, characterized by being prepared by the preparation method of any one of claims 1-6.
  8. 8. The active extract of cherry petals according to claim 7, wherein the content of sakura glycoside in the active extract of cherry petals is not less than 1.2wt%, and/or the content of sakura flavone in the active extract of cherry petals is not less than 0.8wt%, and/or the content of polyphenols in the active extract of cherry petals is not less than 2.5wt%.
  9. 9. The sakura petal active extract according to any one of claims 7-8, wherein the sakura petal active extract has an inhibition rate of propionibacterium acnes of greater than or equal to 85% and/or an acceleration rate of the sakura petal active extract of greater than or equal to 30% of staphylococcus epidermidis.
  10. 10. The active extract of sakura petals according to any one of claims 7-8, wherein the active extract of sakura petals has an absorbance of blue light of at least 80% and/or a free radical clearance of blue light induced of at least 75% in the wavelength range of 400-500 nm.

Description

Preparation method of sakura extract with flora regulation and antioxidation effects Technical Field The invention relates to the technical field of skin care product raw materials, in particular to a preparation method of a sakura extract with flora regulation and antioxidation effects. Background Along with the change of life style, people are exposed to the blue light environment of electronic equipment for a long time (the blue light environment can bring about the problem of skin oxidization), and meanwhile, the problems of sensitivity, acne, dryness and the like caused by unbalanced skin flora are increasingly common, so that the development of skin care product raw materials with flora regulation and antioxidation effects is particularly important. The sakura petals contain rich active ingredients such as sakura glycoside, sakura flavone, polyphenols and the like, and the ingredients have certain antioxidation and anti-inflammatory effects and have good application prospects in the field of skin care products. The existing preparation technology of the sakura extract has a plurality of defects that, for example, the traditional extraction method (such as static leaching and Soxhlet extraction) has low extraction efficiency, the loss of active ingredients is serious, and the specific ingredients related to flora regulation and antioxidation in the sakura petals are difficult to fully extract. Disclosure of Invention The invention aims to provide a preparation method of a sakura extract with flora regulation and antioxidation effects. The method can efficiently extract the active ingredients in the sakura petals, retain the flora regulation and antioxidant activity of the sakura petals, has mild process and is suitable for industrial mass production. In order to achieve the above purpose, the invention adopts the following technical scheme: A preparation method of a sakura extract with flora regulation and antioxidation effects comprises the following steps: S1, raw material pretreatment, namely selecting fresh and mildew-free and pest-free cherry petals, cleaning, draining, vacuum freeze-drying, controlling the moisture content of the dried cherry petals to be 0.5-10wt%, crushing the dried cherry petals, sieving with a 60-80 mesh sieve to obtain cherry petal powder, adding compound enzyme into the cherry petal powder for enzymolysis, wherein the compound enzyme consists of cellulase, pectase and protease according to the weight ratio of 2:0.8-1.1:0.8-1.1, and performing enzyme deactivation treatment after the enzymolysis is finished to obtain the enzymolysis cherry petal powder; s2, carrying out gradient dynamic leaching, namely mixing the enzymatic hydrolysis sakura petal powder with an extraction solvent according to the weight ratio of 1:50-100, wherein the extraction solvent is ethanol water solution with the weight percent of 40-60, ascorbic acid accounting for 0.1-0.3 weight percent and citric acid accounting for 0.05-0.15 weight percent of the ethanol water solution are added into the ethanol water solution, carrying out leaching for 1-2h at 20-30 ℃ by adopting a gradient temperature dynamic leaching mode, carrying out leaching for 1-2h at 40-50 ℃, carrying out leaching for 0.5-1h at 55-60 ℃, carrying out ultrasonic assistance in the leaching process, wherein the ultrasonic power is 150-250W, the ultrasonic frequency is 20-40kHz, each leaching time is 30-35min, stirring for 10-12min, the stirring speed is 150-200r/min, filtering after leaching is finished, and collecting filtrate to obtain a crude extract; S3, purifying, namely centrifuging the crude extract at a speed of 3000-5000r/min for 15-25min, taking supernatant, purifying the supernatant by a macroporous resin column, eluting impurities by deionized water, eluting by 50-70wt% of ethanol water solution, collecting eluent, ultrafiltering the eluent by an ultrafiltration membrane with a molecular weight cut-off of 1000-5000Da, and collecting permeate; S4, concentrating and desolventizing, namely concentrating the permeate liquid in a reduced pressure concentration mode, controlling the concentration temperature to be 40-50 ℃, and the vacuum degree to be-0.08 to-0.06 MPa, and concentrating until the solid content of the concentrate liquid is 20-30wt%; S5, spray drying, namely spray drying the concentrated solution, wherein the air inlet temperature of the spray drying is 160-180 ℃, the air outlet temperature is 80-90 ℃, the feeding speed is 5-15mL/min, and the sakura petal active extract is obtained after drying. Advantageous effects The invention can fully extract the active components (such as sakura glycoside and small molecular peptide) related to flora regulation and the active components (such as sakura leaf flavone and polyphenol) related to antioxidation in sakura petals through the cooperative optimization of the process, wherein the sakura glycoside and the small molecular peptide can regulate the balance of skin flora, inhibit th