Search

CN-122005403-A - Centella asiatica leaf extract with relieving effect and application thereof

CN122005403ACN 122005403 ACN122005403 ACN 122005403ACN-122005403-A

Abstract

The invention discloses a asiatic centella leaf extract with relieving effect and application thereof, wherein the asiatic centella leaf extract is prepared by adding crushed and sieved asiatic centella leaf powder, an auxiliary carbon source and an auxiliary nitrogen source into water, regulating pH to 5.0-7.0, and sterilizing to prepare a fermentation culture medium; inoculating activated compound microorganism strains into a fermentation culture medium for fermentation culture, sterilizing, centrifuging and filtering to obtain the compound microorganism strains, wherein the compound microorganism strains comprise lactobacillus rhamnosus, lactobacillus reuteri and bacillus subtilis. According to the invention, the centella asiatica leaves are fermented and cultured by adopting the composite microorganism strains of the rhamnose cheese bacillus, the lactobacillus reuteri and the bacillus subtilis, and the obtained centella asiatica leaf extract has remarkable anti-inflammatory activity and relieving efficacy. Proved by verification, the content of TNF-alpha and IL-6 can be obviously reduced, and the inhibition rate of the hyaluronidase can be obviously improved.

Inventors

  • CHEN LI
  • LIN MENGGAN
  • SUN XIAOXIAO

Assignees

  • 仁和全域(上海)大健康研究院有限公司

Dates

Publication Date
20260512
Application Date
20260409

Claims (10)

  1. 1. A preparation method of asiatic centella leaf extract with relieving effect is characterized by comprising the following steps: S1, crushing centella leaves and sieving; s2, adding the sieved asiatic centella leaf powder, an auxiliary carbon source and an auxiliary nitrogen source into water, regulating the pH value to 5.0-7.0, and then sterilizing to prepare a sterilized fermentation culture medium; S3, inoculating the activated composite microorganism strain into a fermentation culture medium for fermentation culture; s4, sterilizing after fermentation culture, and centrifuging and filtering to obtain the asiatic centella leaf extract with relieving effect; wherein in step S3, the compound microorganism strain comprises Lactobacillus rhamnosus, lactobacillus reuteri and Bacillus subtilis.
  2. 2. The preparation method of the asiatic centella leaf extract with the soothing effect according to claim 1, wherein the ratio of the number of viable bacteria of lactobacillus rhamnosus, lactobacillus reuteri and bacillus subtilis is 1:1:1 to 4:1:4.
  3. 3. The preparation method of the asiatic pennywort herb extract with the soothing effect according to claim 1, wherein the lactobacillus rhamnosus is lactobacillus rhamnosus LR663, lactobacillus reuteri is lactobacillus reuteri CCFM1154 and bacillus subtilis is bacillus subtilis BNCC188080.
  4. 4. A process for the preparation of a centella asiatica leaf extract with soothing action according to any one of claims 1 to 3, characterized in that the lactobacillus rhamnosus, lactobacillus reuteri and bacillus subtilis are subjected to an activation treatment prior to inoculation, the step of said activation treatment being: Inoculating lactobacillus rhamnosus and lactobacillus reuteri respectively into an MRS liquid culture medium, and culturing for 24-36 hours at 37 ℃ and 100 rpm to obtain activated lactobacillus rhamnosus and activated lactobacillus reuteri; inoculating bacillus subtilis into an LB liquid culture medium, and culturing for 24-36 hours at 37 ℃ and 100 rpm to obtain the activated bacillus subtilis.
  5. 5. The method for preparing a asiatic centella leaf extract with soothing function according to claim 1, wherein in the step S1, the asiatic centella leaf is crushed and then sieved by a 60-80 mesh sieve.
  6. 6. The method for preparing a asiatic centella leaf extract with soothing effect according to claim 1, wherein in the step S2, the auxiliary carbon source is glucose and the auxiliary nitrogen source is peptone; In the step S2, the addition amount of the asiatic centella leaf powder is 50-70 g/L; the addition amount of the auxiliary carbon source and the auxiliary nitrogen source is 1-5% of the dry weight of the asiatic centella leaf powder.
  7. 7. The method for preparing centella asiatica leaf extract with soothing effect according to claim 1, wherein in step S2, pH is adjusted by adding phosphate.
  8. 8. The method for preparing a asiatic centella leaf extract with soothing function according to claim 1, wherein the inoculation amount of the compound microorganism strain in the step S3 is 5-10%.
  9. 9. The preparation method of the asiatic centella leaf extract with relieving effect according to claim 1, wherein in the step S3, the fermentation culture condition is that fermentation is carried out for 48-72 h under the aerobic condition at 25-37 ℃.
  10. 10. Use of an extract of centella asiatica leaves prepared according to the method of any one of claims 1-9 for the preparation of a cosmetic having soothing effect.

Description

Centella asiatica leaf extract with relieving effect and application thereof Technical Field The invention belongs to the technical field of cosmetics, and particularly relates to a asiatic centella leaf extract with a relieving effect and application thereof. Background Centella asiatica is a dried whole herb of centella asiatica CENTELLA ASIATICA (L.) Urban belonging to the genus centella of the family Umbelliferae, belonging to perennial herb, and is a traditional medicinal plant. Centella asiatica is widely distributed in Jiangsu, zhejiang, anhui, guangdong, guangxi, hunan and other places. Centella asiatica is originally carried in Shen nong Ben Cao Jing, and has the effects of clearing heat and promoting diuresis, and removing toxic substances and relieving swelling, and is cold in nature, bitter in taste and pungent in flavor, and enters liver, spleen and kidney essence. It is often used to treat jaundice due to damp-heat, carbuncle, swelling, sore, and traumatic injury. The main chemical components of the centella asiatica leaves are triterpenes, volatile oil, polyacetylenes and the like, and the centella asiatica leaves contain various medicinal active components such as asiaticoside, madecassoside, asiatic acid and the like, and have the effects of resisting inflammation, promoting wound healing, resisting oxidation, relieving skin and the like. In recent years, with the development of more and more medicinal plants, centella asiatica is also widely used in cosmetics and medicines, and most of added raw materials are centella asiatica extract liquid, centella asiatica extract powder or high-content madecassoside, and the extracts are generally subjected to complex extraction processes to obtain finished products, however, the traditional extraction methods (such as water extraction and alcohol extraction) may have the following defects that (1) part of active ingredients are not high in extraction rate or large in molecular weight and are not easy to be absorbed transdermally, (2) potential active substances in plant cells may not be completely released, and (3) the stability of the extracted substances is required to be improved. The microbial fermentation technology provides a new way for the deep development of plant raw materials. Microorganisms generally contain several active enzymes, including amylase, beta-glucosidase, cellulase, protease, lipase, etc., and under the action of the enzymes, the microorganisms decompose or convert underutilized substrates into compatible moieties, and can also exert efficacy by promoting the production or conversion of active ingredients into their metabolites, increasing the antioxidant content, or decomposing macromolecular substances into small molecular substances, so that the active substances are better absorbed by the human body. The natural product extract prepared by the microbial fermentation process can effectively reduce the inherent toxicity of some natural products. Research shows that macromolecular substances can be decomposed into small molecules which are easier to absorb through fermentation, and new active metabolites are generated, so that various efficacy activities are obviously improved. The microbial fermentation broth also has the characteristics of low cost, difficult sensitization and the like, and is a hotspot for research and development of current development. However, at present, few reports are made on the symbiotic fermentation of centella leaves, especially by adopting specific strains, so as to obtain a fermentation liquor product with synergistic enhancement and relief and repair effects. In recent years, people with sensitive skin problems are rapidly increasing and are more and more younger, and the problems of sensitive skin and easy inflammation of skin are widely focused by people due to the influence of factors such as environmental pollution, increased living pressure, unhealthy living modes and the like. The international pruritus study forum (IFSI) and the national institute of sensitive skin diagnosis and treatment (2017 edition) are integrated, and sensitive skin is defined as a syndrome of paroxysmal or periodic burning, flushing, stinging, itching and tightening after the skin is subjected to external micro-stimulation, with or without persistent erythema. Sensitive skin is most likely to occur on the face and is a highly intolerant skin state, and usually the skin is subjected to some minor stimuli such as temperature change, seasonal change and the like, and then is subjected to symptoms such as burning, paroxysmal redness, stinging, itching and the like with or without erythema, so that the skin is often intolerant to common skin care products, and great trouble is caused to the problem population. The problems of light aging and inflammation after sun exposure are also concerns due to the influence of seasonal ultraviolet radiation. The therapeutic principle is to control symptoms, promote skin barrier re