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CN-122005404-A - Preparation method of purslane extract with relieving effect

CN122005404ACN 122005404 ACN122005404 ACN 122005404ACN-122005404-A

Abstract

The invention relates to a preparation method of purslane extract with a relieving effect, belonging to the field of cosmetics and biological extraction. Pulverizing fresh herba Portulacae, adding butanediol-water mixed solvent, performing ultrasonic wall breaking at 55-58 deg.C, adding plant fiber hydrolase, plant gum hydrolase, plant starch hydrolase and plant protein hydrolase, performing synergistic enzymolysis, inactivating by heating, filtering, sequentially performing chitosan flocculation, active carbon decolorizing, ceramic membrane filtering, ultrafiltering and nanofiltration, collecting the retentate with molecular weight of more than 150-300 Da, and adding humectant and antiseptic. The active ingredients such as polysaccharide, flavone and the like in the purslane are efficiently reserved under mild conditions by combining ultrasonic-enzymolysis synergistic extraction and multistage membrane separation and purification, the inhibition rate of the obtained extract on the hyaluronidase activity reaches more than 20%, the extract has a relieving effect, and the product has good stability and can be applied to relieving cosmetics or skin care products.

Inventors

  • Liang Shanwen
  • YANG HAOFENG

Assignees

  • 佛山市捷冠生物科技有限公司

Dates

Publication Date
20260512
Application Date
20260411

Claims (10)

  1. 1. A preparation method of purslane extract with relieving effect is characterized by comprising the following steps: S1, crushing fresh wild purslane to obtain purslane pulp; S2, adding 50-90% of an extraction solvent into the purslane pulp, wherein the extraction solvent is a mixed solution of butanediol and water, the volume fraction of the butanediol is 20-50%, the volume fraction of the water is 80-50%, heating to 55-58 ℃, performing ultrasonic wall breaking treatment, and then adding plant fiber hydrolase, plant gum hydrolase, plant starch hydrolase and plant protein hydrolase, and stirring and extracting to obtain an extract; S3, heating and inactivating the extracting solution, and filtering to obtain filtrate I; s4, adding a chitosan flocculant into the filtrate I, and filtering after flocculation to obtain filtrate II; s5, decolorizing the filtrate II by using active carbon, and filtering by using a ceramic membrane after solid-liquid separation to obtain filtrate; S6, ultrafiltering the filtrate obtained in the step S5 by using an ultrafiltration membrane with the molecular weight cutoff of 10000 Da, collecting the permeate, then carrying out nanofiltration by using a nanofiltration membrane with the molecular weight cutoff of 150-300 Da, and collecting the retentate to obtain filtrate III; And S7, adding a humectant and a preservative into the filtrate III to obtain the purslane extracting solution.
  2. 2. The method according to claim 1, wherein the ultrasonic wall breaking treatment in step S2 is performed for 20 to 40 minutes.
  3. 3. The method according to claim 1, wherein the plant fiber hydrolase, the plant gum hydrolase, the plant starch hydrolase and the plant protein hydrolase are added in the amount of 1 to 3 per mill, respectively, in the step S2.
  4. 4. The method according to claim 1, wherein the time of the stirring extraction in step S2 is 2 to 4 hours.
  5. 5. The method according to claim 1, wherein the temperature of the heating inactivation in step S3 is 85 ℃ to 95 ℃ for 5 to 15 minutes.
  6. 6. The preparation method according to claim 1, wherein the chitosan flocculant in the step S4 is added in a mass fraction of 0.1% -0.5% and the flocculation time is 20-40 minutes.
  7. 7. The preparation method according to claim 1, wherein the activated carbon is added in the step S5 in a mass fraction of 0.3% -0.8%, the decoloring temperature is 20-60 ℃, the decoloring time is 30-60 minutes, and the pore diameter of the ceramic membrane is 0.05-0.2 μm.
  8. 8. The method according to claim 1, wherein the ultrafiltration membrane in step S6 has a molecular weight cut-off of 10000 Da and the nanofiltration membrane has a molecular weight cut-off of 150 to 300 Da.
  9. 9. A purslane extract prepared according to the preparation method of any one of claims 1 to 8.
  10. 10. Use of the purslane extract according to claim 9 for preparing cosmetics or skin care products with soothing efficacy.

Description

Preparation method of purslane extract with relieving effect Technical Field The invention relates to the technical field of cosmetics and biological extraction, in particular to a preparation method of purslane extract with a relieving effect. Background Herba Portulacae (Portulaca oleracea L.) as traditional medicinal plant is rich in polysaccharide, flavonoid, alkaloid and organic acid, and has biological activities such as antioxidant, antiinflammatory, antibacterial and immunoregulatory effects, and has wide application value in medicine, functional food and cosmetic fields. Among them, purslane extract has been a research hotspot of functional cosmetic raw materials due to its good soothing, antiallergic and repairing effects. At present, the preparation method of the purslane extract mainly comprises a water extraction method, an alcohol extraction method, microwave-assisted extraction, ultrasonic-assisted extraction and the like. However, the prior art has the common problems that the traditional hot water extraction method has higher temperature (usually 80-100 ℃) and is easy to cause degradation or oxidation of thermosensitive active ingredients (such as polysaccharide and flavonoid glycoside) and reduce the biological activity of the extract, the alcohol extraction method can improve the extraction rate of partial fat-soluble ingredients, but has high residual risk of organic solvents, has lower extraction efficiency on water-soluble active ingredients (such as purslane polysaccharide), and the single ultrasonic or microwave-assisted extraction can improve the extraction efficiency to a certain extent, but the cell wall breaking is incomplete, the active ingredients are not released sufficiently, and the extraction rate still has larger lifting space. In addition, the existing extraction process mostly adopts simple filtration or single-stage purification, the content of impurities in the obtained extract is higher, the purity of active ingredients is low, and the relieving efficacy and stability of the product are directly affected. Aiming at the technical problems, although researches and reports on the adoption of enzymolysis auxiliary extraction are presented in the prior art, the method is mostly limited to single enzyme or simple enzymolysis, and lacks a multi-enzyme synergistic system design aiming at a purslane cell wall complex structure (a multi-layer composite structure containing cellulose, hemicellulose, pectin, starch, protein and the like), and in a purification link, the conventional activated carbon decolorization or single membrane filtration is difficult to meet the double requirements of impurity removal and active ingredient enrichment, so that the content fluctuation of active ingredients in a product is large, and the relieving effect is unstable. Therefore, how to realize efficient extraction, accurate purification and activity retention of the active ingredients of purslane under mild conditions, and obtain a purslane extract which is stable and controllable and has obvious relieving effect is still a technical problem to be solved in the art. Disclosure of Invention The invention provides a preparation method of purslane extract with a relieving effect, aiming at solving the problems in the prior art. In order to solve the technical problems, the invention is realized by the following technical scheme that the preparation method of the purslane extract with the relieving effect comprises the following steps: S1, crushing fresh wild purslane to obtain purslane pulp; S2, adding 50-90% of an extraction solvent into the purslane pulp, wherein the extraction solvent is a mixed solution of butanediol and water, the volume fraction of the butanediol is 20-50%, the volume fraction of the water is 80-50%, heating to 55-58 ℃, performing ultrasonic wall breaking treatment, and then adding plant fiber hydrolase, plant gum hydrolase, plant starch hydrolase and plant protein hydrolase, and stirring and extracting to obtain an extract; S3, heating and inactivating the extracting solution, and filtering to obtain filtrate I; s4, adding a chitosan flocculant into the filtrate I, and filtering after flocculation to obtain filtrate II; s5, decolorizing the filtrate II by using active carbon, and filtering by using a ceramic membrane after solid-liquid separation to obtain filtrate; S6, ultrafiltering the filtrate obtained in the step S5 by using an ultrafiltration membrane with the molecular weight cutoff of 10000 Da, collecting the permeate, then carrying out nanofiltration by using a nanofiltration membrane with the molecular weight cutoff of 150-300 Da, and collecting the retentate to obtain filtrate III; And S7, adding a humectant and a preservative into the filtrate III to obtain the purslane extracting solution. In a specific embodiment of the first aspect, the time of the ultrasonic wall breaking treatment in step S2 is 20 to 40 minutes. In a specific embodiment of the first aspect,