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CN-122005405-A - Oil-soluble plant compound and preparation method thereof

CN122005405ACN 122005405 ACN122005405 ACN 122005405ACN-122005405-A

Abstract

The invention provides an oil-soluble plant compound and a preparation method thereof, belonging to the field of medical, dental or cosmetic preparations. The composition comprises flos Matricariae Chamomillae, rhizoma Ligustici Chuanxiong, olibanum, exocarpium Citri Grandis essential oil and soybean-radix Puerariae co-fermented product. The technology core is that Aspergillus oryzae is utilized to carry out synergistic liquid fermentation on soybean and radix puerariae, lysophospholipid, soybean polypeptide and puerarin aglycone are generated in situ through biotransformation, and an endogenous self-emulsifying carrier is constructed. The process utilizes the regulating effect of the ligusticum chuanxiong oil on lipid arrangement of the horny layer, and combines the micro micelle structure formed by the co-fermentation system, thereby remarkably enhancing the transdermal penetration capability of the active components. The invention not only realizes the nanoscale self-solubilization of four essential oil matrixes in the fermentation matrix, but also enhances the skin barrier repair and inflammation inhibition effects through multi-target-point synergy, thereby greatly improving the bioavailability, penetration depth and safety of the active ingredients.

Inventors

  • CEN SHUIBIN
  • YI GUANG
  • ZHANG ZHENXIAN
  • HUANG MINYU

Assignees

  • 广东轻工职业技术大学
  • 同康生命科学(广州)有限公司

Dates

Publication Date
20260512
Application Date
20260413

Claims (10)

  1. 1. The plant composition is characterized by comprising the following components in parts by mass: Golden chamomile extract 5-12 Ligusticum wallichii oil 2-5 3-8 Parts of rosewood oil Pummelo peel essential oil 1-2 70-90% Of soybean-kudzuvine root co-fermented product; The soybean-kudzuvine root co-ferment is obtained by mixing soybean and kudzuvine root according to a mass ratio of 3-5:1, inoculating aspergillus oryzae for fermentation, and extracting by a medium-low polarity solvent.
  2. 2. The plant composition according to claim 1, wherein the soybean-kudzuvine root co-fermented product is refined by ultrafiltration membrane with a molecular weight cut-off of 10000Da after extraction with medium-low polarity solvent, and the permeate is taken and concentrated under reduced pressure until the solid content is not less than 30%.
  3. 3. The plant composition according to claim 1, wherein the golden chamomile extract, the chuanxiong rhizome oil, the cream oil, the pummelo peel essential oil are alcohol-soluble oily components obtained by physical separation or solvent extraction, respectively, from the respective plants.
  4. 4. The plant composition according to claim 1, wherein the preparation method of the soybean-kudzuvine root co-fermented product comprises the following steps, S1, taking defatted soybeans and kudzuvine root, drying, pulverizing into powder, mixing, adding water according to a feed-liquid ratio of 1:8-12, adding at least 0.2% (w/w) of monopotassium phosphate and at least 0.1% (w/w) of magnesium sulfate heptahydrate according to the total mass of the liquid, regulating the initial pH value to be between 5.5 and 6.0, and sterilizing to obtain a fermentation substrate; s2, inoculating aspergillus oryzae into an induction culture medium containing at least 1% (w/w) of soybean lecithin for induction culture, and preparing seed liquid; s3, inoculating Aspergillus oryzae seed liquid into a fermentation substrate, and fermenting according to the following parameters; The temperature is 30+/-1 ℃, the ventilation is 1.0-1.5VVM, and the fermentation time is at least 60 hours; s4, inactivating after fermentation is finished, and centrifuging to obtain supernatant, wherein the supernatant is added with ethanol aqueous solution for extraction, and an ethanol extract is obtained; S5, the alcohol extract passes through an ultrafiltration membrane with the molecular weight cut-off of 10000Da, the permeate is collected, and the permeate is decompressed and concentrated until the solid content is more than or equal to 30 percent.
  5. 5. The plant composition according to claim 4, wherein in step S3, the inoculum size of the aspergillus oryzae seed liquid is at least 5% of the substrate volume.
  6. 6. The plant composition according to claim 4, wherein in the step S4, fermentation is completed under the condition that the fermentation broth has a pH value at the lowest point and has a rise, wherein the rise is at least 0.2 units, and the final pH value is not less than 4.3.
  7. 7. The plant composition according to claim 4, wherein in the step S4, the ethanol water is added in an amount of 2 to 3 times the volume of the supernatant and the concentration of the ethanol water is 75 to 95% when the supernatant is extracted with the ethanol aqueous solution.
  8. 8. A process for the preparation of a plant composition as claimed in any one of claims 1 to 7, characterized in that it comprises the following steps, S1, preheating soybean-kudzuvine root co-fermented products to 40-55 ℃ and keeping stirring; S2, mixing the rest components, and adding the mixture into the soybean-kudzuvine root co-fermentation product in a stirring state to obtain a crude mixed solution; and S3, homogenizing the crude mixed solution to obtain the plant composition.
  9. 9. Use of a phytocomposition according to any one of claims 1 to 8 for the preparation of cosmetics.
  10. 10. The use according to claim 9, wherein the plant composition is present in an amount of 1% or more of the cosmetic product.

Description

Oil-soluble plant compound and preparation method thereof Technical Field The invention belongs to the field of medical, dental or cosmetic preparations, and particularly relates to an oil-soluble plant compound and a preparation method thereof. Background In the fields of modern cosmetics and biotechnology, the research and development of products for relief and repair always takes a central position. With the increasing density of sensitive skin populations and the generalization of the problem of impaired skin barriers, the development of repair compositions with high-efficiency bioactivity has become an important direction of industrial technological innovation. Traditional repair products mostly rely on simple compounding of plant extracts or addition of synthetic chemical components. However, under the prior art conditions, how to achieve efficient loading, stable release and improvement of bioavailability of the natural active ingredient still faces multiple technical bottlenecks. The plant essential oil is taken as a natural component with remarkable biological activity, has a small molecular weight and strong penetrability, has a multidimensional biological function, and has great potential in the field of skin repair. However, in practical applications, essential oil substances have extremely strong hydrophobicity and instability. The prior art for introducing these non-polar oily components into aqueous or emulsion systems generally requires the addition of large amounts of synthetic surfactants, chemical cosolvents, or high concentrations of polyols. Although these exogenous additives solve the solubility problem at the physical level, they bring about a remarkable technical negative effect, on the one hand, the high dose of synthetic emulsifier may destroy the arrangement of the skin self lipid barrier, creating a secondary irritation risk, contrary to the original purpose of developing the maintenance product, and on the other hand, the complex chemical co-solvent system tends to interfere with the structural stability of the plant active molecules, reducing their expression of biological activity on the skin surface. Meanwhile, biological fermentation technology has been widely used for raw material processing as an important means for improving plant efficiency. Traditional fermentation processes focus on the conversion of a single plant substrate, the functional dimensions of the product are relatively single, and the metabolites in the fermentation process often lack sufficient physicochemical characteristics to support the carrying requirements for other active components. In the field of synergistic effect research of plant materials, the prior art focuses on physical superposition of functional components, and lacks systematic construction from the aspects of microbial metabolism induction and intermolecular interaction. For example, whether or not different classes of plant substrates are metabolically coupled during co-fermentation, and whether or not the endogenous metabolites produced by fermentation can form spontaneous physical entrapment systems with essential oil components of a specific polarity, have remained lacking in the current literature in profound theoretical support and maturing practices. Particularly, under the large trend of the industry pursuing green chemistry and simplified formulation, how to utilize a bioconversion means to generate biomolecules with interfacial activity in situ, so as to replace synthetic chemicals to realize self-solubilization of essential oil is a key subject to be overcome in the current cosmetic raw material field. In view of the above, it is still difficult to achieve a perfect balance between naturalness and stability, high activity and low irritation, and physical compatibility between complex components in the existing soothing composition. Disclosure of Invention The first object of the invention is to provide a plant composition, which comprises the following components in parts by mass: Golden chamomile extract 5-12 Ligusticum wallichii oil 2-5 3-8 Parts of rosewood oil Pummelo peel essential oil 1-2 70-90% Of soybean-kudzuvine root co-fermented product; The soybean-kudzuvine root co-ferment is obtained by mixing soybean and kudzuvine root according to a mass ratio of 3-5:1, inoculating aspergillus oryzae for fermentation, and extracting by a medium-low polarity solvent. Preferably, the soybean-kudzuvine root co-fermented product is refined by an ultrafiltration membrane with a cut-off molecular weight of 10000Da after being extracted by a medium-low polarity solvent, and the permeate is taken and concentrated under reduced pressure until the solid content is more than or equal to 30 percent. Preferably, the golden chamomile extract, the ligusticum wallichii oil, the cream oil and the pummelo peel essential oil are respectively alcohol-soluble oily components obtained by physical separation or solvent extraction from respective pla