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CN-122005415-A - An antiaging composition containing silkworm extract, and its preparation method and application

CN122005415ACN 122005415 ACN122005415 ACN 122005415ACN-122005415-A

Abstract

The invention relates to an anti-aging composition containing silkworm extract, and a preparation method and application thereof, belonging to the technical field of cosmetics. The anti-aging composition comprises hydroxypropyl cyclodextrin, liposome and an anti-aging active ingredient loaded in the liposome, wherein the anti-aging active ingredient comprises ergothioneine, resveratrol and silkworm extract, and the three ingredients have synergistic effect, so that the anti-aging composition can enhance the resistance to oxidative stress, inhibit collagen degradation and reduce cell damage. Furthermore, the liposome prepared from phosphatidylcholine, polyquaternium-61 and brassinosteroids is used for loading the anti-aging active ingredients, so that the stability and transdermal performance of the anti-aging active ingredients are improved, and the liposome effectively conveys the active ingredients to the deep skin layer through breaking through the stratum corneum barrier, so that the bioavailability is improved, and further the effects of long-acting antioxidation, aging delay and the like are realized.

Inventors

  • CHU RUIXUE
  • HUANG XIAOSHAN

Assignees

  • 广珠(深圳)生物科技有限公司
  • 广州科丸生物科技有限公司

Dates

Publication Date
20260512
Application Date
20260331

Claims (10)

  1. 1. The anti-aging composition is characterized by comprising hydroxypropyl cyclodextrin, liposome and an anti-aging active ingredient loaded in the liposome, wherein the liposome ingredient comprises phosphatidylcholine, polyquaternium-61 and campesterol, the anti-aging active ingredient comprises ergothioneine, resveratrol and silkworm extract, the weight ratio of the hydroxypropyl cyclodextrin, the phosphatidylcholine, the polyquaternium-61, the campesterol, the ergothioneine, the resveratrol and the silkworm extract is 5-10:3-5:0.5-1:1:0.5-1:0.5-1.5:1-3, and the resveratrol is obtained by taking giant knotweed as a raw material and performing liquid fermentation and purification on the mixture of green mold and kluyveromyces marxianus.
  2. 2. The anti-aging composition of claim 1, wherein the method of preparing the anti-aging composition comprises the steps of: S1, dissolving phosphatidylcholine, polyquaternium-61, brassinosteroids and resveratrol in absolute ethyl alcohol to obtain an oil phase; s2, dissolving ergothioneine and silkworm extract in ultrapure water, regulating the pH value to 7.0, and hydrating to obtain a water phase; s3, injecting the oil phase into the water phase, uniformly mixing, homogenizing under high pressure to obtain a liposome solution, and removing ethanol by rotary evaporation to obtain a liposome concentrated solution; s4, dissolving hydroxypropyl cyclodextrin in ultrapure water, adding the liposome concentrated solution, uniformly mixing, and spray drying to obtain the anti-aging composition.
  3. 3. The anti-aging composition according to claim 2, wherein the concentration of the sterols in the oil phase in step S1 is 1wt% to 2wt%, and the dissolution is a stirring dissolution at room temperature at a rotational speed of 300 to 400 r/min.
  4. 4. The anti-aging composition according to claim 2, wherein the dissolution in step S2 is stirring dissolution at room temperature at a rotational speed of 300-400r/min, the hydration is hydration at 4 ℃ for 8-12h, and the content of ergothioneine in the aqueous phase is 0.5-1 wt%.
  5. 5. The anti-aging composition according to claim 2, wherein the mixing in step S3 is carried out at a high speed of 5-10min at a rotation speed of 8000-12000r/min, the high-pressure homogenization is carried out at 50-80MPa for 5-8 times each for 1.5min, and the rotary evaporation is carried out at a vacuum of 0.10-0.15MPa and a temperature of 40-48 ℃.
  6. 6. The anti-aging composition of claim 1, wherein the resveratrol is prepared by a process comprising the steps of: (1) Pulverizing rhizoma Polygoni Cuspidati, sieving to obtain rhizoma Polygoni Cuspidati powder, mixing rhizoma Polygoni Cuspidati powder with deionized water at a weight ratio of 1:10-20, performing hydrothermal extraction, adding glucose, peptone and sodium chloride into the obtained extractive solution, and sterilizing to obtain fermentation substrate; (2) Inoculating 0.5-2% v/v of mixed bacteria agent consisting of penicillium liquid and Kluyveromyces marxianus liquid with the volume ratio of 1:0.1-0.5 into a fermentation substrate for fermentation to obtain fermentation liquor; (3) Centrifuging the fermentation liquor, taking supernatant, adsorbing, eluting and filtering by macroporous resin, collecting eluting filtrate, and freeze-drying to obtain resveratrol.
  7. 7. The anti-aging composition of claim 6, wherein the hydrothermal extraction is performed at 50-60 ℃ for 2-4 hours, and the fermentation substrate comprises 1wt% to 2wt% glucose, 1wt% to 2wt% peptone, and 0.5wt% to 0.8wt% sodium chloride.
  8. 8. The composition according to claim 6, wherein the penicillium liquid in the step (2) contains 1X 10 5 -2×10 5 spores per mL, the viable count of the Kluyveromyces marxianus liquid is 1X 10 6 -2×10 6 CFU per mL, and the fermentation is carried out at 28-32 ℃ for 48-72h under shaking at 150-180 r/min.
  9. 9. The anti-aging composition of claim 6, wherein the macroporous resin in step (3) is a NKA-2 macroporous resin, the eluting is performed using 65% v/v to 80% v/v aqueous ethanol, and the filtering is performed using 0.22 μm filter paper.
  10. 10. Use of an anti-ageing composition as claimed in any of claims 1 to 9 in the preparation of a cosmetic product.

Description

An antiaging composition containing silkworm extract, and its preparation method and application Technical Field The invention relates to the technical field of cosmetics, in particular to an anti-aging composition containing silkworm extract, a preparation method and application thereof. Background Skin aging is jointly initiated by multiple factors such as free radical oxidative damage, insufficient collagen synthesis, skin barrier function decline and the like, and the compound active component with the effects of powerful antioxidation, targeted anti-wrinkle and barrier repair is a core research and development direction in the current cosmetic anti-aging field. The active ingredients for resisting aging at present mainly comprise natural polyphenol active substances which can effectively remove oxygen free radicals, resist photoaging and inhibit oxidative stress injury of skin, and polypeptide active ingredients which can target and regulate skin metabolic signals, promote synthesis of collagen and elastin, lighten dynamic fine wrinkles and repair damaged skin barriers. However, the active ingredients have some defects in practical research and development and industrialized application, such as poor stability and low bioavailability of the active ingredients, and most of the anti-aging active ingredients are sensitive to light, oxygen and temperature, are easily oxidized, degraded and inactivated by direct addition, are difficult to penetrate the stratum corneum of the skin, cannot reach effective concentration at an action target point, and have great discount on the anti-aging effect. Therefore, development of an anti-aging product which can stably and efficiently deliver active ingredients and realize multi-target synergistic anti-aging is needed. Disclosure of Invention The invention aims to overcome the defects of the prior art and provide an anti-aging composition containing silkworm extract, and a preparation method and application thereof. In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: The invention provides an anti-aging composition, which comprises hydroxypropyl cyclodextrin, liposome and an anti-aging active ingredient loaded in the liposome, wherein the liposome comprises phosphatidylcholine, polyquaternium-61 and campesterol, the anti-aging active ingredient comprises ergothioneine, resveratrol and silkworm extract, the weight ratio of the hydroxypropyl cyclodextrin, the phosphatidylcholine, the polyquaternium-61, the campesterol, the ergothioneine, the resveratrol and the silkworm extract is 5-10:3-5:0.5-1:1:0.5-1.5:1, and the resveratrol is obtained by taking giant knotweed as a raw material and performing liquid fermentation and purification on the mixture of green mold and kluyveromyces marxianus. The anti-aging composition provided by the invention comprises active ingredients of ergothioneine, resveratrol and silkworm extract. Ergothioneine can be used as a natural antioxidant in cells, can directly remove Reactive Oxygen Species (ROS), protect mitochondrial DNA from oxidative damage, and maintain functions of a cell energy factory. The resveratrol is prepared by mixing the penicillium and the Kluyveromyces marxianus of a specific strain according to a specific dosage proportion to obtain the fermentation microbial agent liquid fermentation polygonum cuspidatum extract, the content of the resveratrol in the polygonum cuspidatum root extract is remarkably improved, the resveratrol is obtained efficiently, the resveratrol has the functions of resisting oxidation, resisting photo-aging, whitening and resisting aging, an SIRT1 longevity gene can be activated, the expression of endogenous antioxidant enzymes (SOD and CAT) is up-regulated, and the skin self antioxidant defense network is enhanced. The silkworm extract contains NAMPT (nicotinamide phosphoribosyl transferase), which can increase NAD + synthesis, provide energy support for antioxidation reaction, and delay cell aging. Therefore, ergothioneine, resveratrol and silkworm extract form a closed loop of scavenging free radicals, strengthening self-protection and supplementing antioxidant energy, and can resist oxidative stress in all directions, inhibit collagen degradation and reduce cell injury. Furthermore, the liposome prepared from phosphatidylcholine, polyquaternium-61 and brassinosteroids is used for loading the anti-aging active ingredients, so that the stability and transdermal performance of the anti-aging active ingredients are improved, and the liposome effectively conveys the active ingredients to the deep skin layer through breaking through the stratum corneum barrier, so that the bioavailability is improved, and the effects of long-acting antioxidation and aging delay are realized. Further, the preparation method of the anti-aging composition comprises the following steps: S1, dissolving phosphatidylcholine, polyquaternium-61, brassinosteroids and resveratrol in a